Handbook of fluorescence spectroscopy and imaging : from single molecules to ensembles / / Markus Sauer, Johan Hofkens, and Jorg Enderlein

• Autore: Sauer Markus
• Pubblicazione: Weinheim, : Wiley-VCH, 2011
• Descrizione fisica: 1 online resource (293 p.)
• Disciplina: 543.56
• Soggetto topico: Fluorescence spectroscopy; Fluorescence microscopy
• Altri autori: HofkensJohan EnderleinJ (Jorg)
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Handbook of Fluorescence Spectroscopy and Imaging: From Single Molecules to Ensembles; Contents; Preface; 1 Basic Principles of Fluorescence Spectroscopy; 1.1 Absorption and Emission of Light; 1.2 Spectroscopic Transition Strengths; 1.3 Lambert-Beer Law and Absorption Spectroscopy; 1.4 Fluorophore Dimerization and Isosbestic Points; 1.5 Franck-Condon Principle; 1.6 Temperature Effects on Absorption and Emission Spectra; 1.7 Fluorescence and Competing Processes; 1.8 Stokes Shift, Solvent Relaxation, and Solvatochroism; 1.9 Fluorescence Quantum Yield and Lifetime; 1.10 Fluorescence Anisotropy ; References2 Fluorophores and Fluorescent Labels; 2.1 Natural Fluorophores; 2.2 Organic Fluorophores; 2.3 Different Fluorophore Classes; 2.4 Multichromophoric Labels; 2.5 Nanocrystals; References; 3 Fluorophore Labeling for Single-Molecule Fluorescence Spectroscopy (SMFS); 3.1 In Vitro Fluorescence Labeling; 3.2 Fluorescence Labeling in Living Cells; References; 4 Fluorophore Selection for Single-Molecule Fluorescence Spectroscopy (SMFS) and Photobleaching Pathways; References; 5 Fluorescence Correlation Spectroscopy; 5.1 Introduction; 5.2 Optical Set-Up; 5.3 Data Acquisition and Evaluation ; 5.4 Milliseconds to Seconds: Diffusion and Concentration5.4.1 Single-Focus FCS; 5.4.2 Dual-Focus FCS; 5.5 Nanoseconds to Microseconds: Photophysics, Conformational Fluctuations, Binding Dynamics; 5.6 Picoseconds to Nanoseconds: Rotational Diffusion and Fluorescence Antibunching; 5.6.1 Antibunching; 5.6.2 Rotational Diffusion; 5.7 Fluorescence Lifetime Correlation Spectroscopy; 5.8 Conclusion; References; 6 Excited State Energy Transfer; 6.1 Introduction; 6.2 Theory of (F€orster) Energy Transfer; 6.2.1 Mechanism and Mathematical Formalism of FRET ; 6.2.2 Measuring FRET Efficiencies Through Excited-State Lifetimes6.2.3 Spin Rules for FRET; 6.2.4 Homo-FRET and FRET-Induced Depolarization; 6.3 Experimental Approach for Single-Pair FRET-Experiments; 6.3.1 Single-Laser Excitation; 6.3.2 Alternating-Laser Excitation (ALEX); 6.4 Examples and Applications of FRET; 6.4.1 FRET Processes in Bulk Experiments; 6.4.1.1 FRET-Based Molecular Biosensors; 6.4.1.2 Energy Hopping and Trapping in Chromophore-Substituted Polyphenylene Dendrimers; 6.4.2 Single-Molecule Observation of FRET; 6.4.2.1 Light-Harvesting Systems: Phycobilisomes and Allophycocyanins ; 6.4.2.2 Hairpin Ribozyme Dynamics and Activity6.4.2.3 Protein (Un)folding and Dynamics; References; 7 Photoinduced Electron Transfer (PET) Reactions; 7.1 Fluorescence Quenching by PET; 7.2 Single-Molecule Fluorescence Spectroscopy to Study PET; 7.3 Single-Molecule Sensitive Fluorescence Sensors Based on PET; 7.4 PET Reporter System; 7.5 Monitoring Conformational Dynamics and Protein Folding by PET; 7.6 Biological and Diagnostic Applications; References; 8 Super-Resolution Fluorescence Imaging; 8.1 Diffraction Barrier of Optical Microscopy ; 8.2 Multi-Photon and Structured Illumination Microscopy
• Sommario/riassunto: Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging.Examples show how each technique can help in obtaining detailed and refine
• Titolo autorizzato: Handbook of fluorescence spectroscopy and imaging
• ISBN: 3-527-63352-9; 1-283-14063-2; 9786613140630; 3-527-63351-0; 3-527-63350-2
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Lingua di pubblicazione: Inglese
• Record Nr.: 9910133642003321
• Lo trovi qui: Univ. Federico II