05392nam 2200637 a 450 991013364200332120200520144314.03-527-63352-91-283-14063-297866131406303-527-63351-03-527-63350-2(CKB)3400000000000399(EBL)645014(OCoLC)705353476(SSID)ssj0000476935(PQKBManifestationID)12202762(PQKBTitleCode)TC0000476935(PQKBWorkID)10495905(PQKB)11490022(MiAaPQ)EBC645014(EXLCZ)99340000000000039920120111d2011 uy 0engur|n|---|||||txtccrHandbook of fluorescence spectroscopy and imaging from single molecules to ensembles /Markus Sauer, Johan Hofkens, and Jorg EnderleinWeinheim Wiley-VCH20111 online resource (293 p.)Description based upon print version of record.3-527-31669-8 Includes bibliographical references and index.Handbook of Fluorescence Spectroscopy and Imaging: From Single Molecules to Ensembles; Contents; Preface; 1 Basic Principles of Fluorescence Spectroscopy; 1.1 Absorption and Emission of Light; 1.2 Spectroscopic Transition Strengths; 1.3 Lambert-Beer Law and Absorption Spectroscopy; 1.4 Fluorophore Dimerization and Isosbestic Points; 1.5 Franck-Condon Principle; 1.6 Temperature Effects on Absorption and Emission Spectra; 1.7 Fluorescence and Competing Processes; 1.8 Stokes Shift, Solvent Relaxation, and Solvatochroism; 1.9 Fluorescence Quantum Yield and Lifetime; 1.10 Fluorescence AnisotropyReferences2 Fluorophores and Fluorescent Labels; 2.1 Natural Fluorophores; 2.2 Organic Fluorophores; 2.3 Different Fluorophore Classes; 2.4 Multichromophoric Labels; 2.5 Nanocrystals; References; 3 Fluorophore Labeling for Single-Molecule Fluorescence Spectroscopy (SMFS); 3.1 In Vitro Fluorescence Labeling; 3.2 Fluorescence Labeling in Living Cells; References; 4 Fluorophore Selection for Single-Molecule Fluorescence Spectroscopy (SMFS) and Photobleaching Pathways; References; 5 Fluorescence Correlation Spectroscopy; 5.1 Introduction; 5.2 Optical Set-Up; 5.3 Data Acquisition and Evaluation5.4 Milliseconds to Seconds: Diffusion and Concentration5.4.1 Single-Focus FCS; 5.4.2 Dual-Focus FCS; 5.5 Nanoseconds to Microseconds: Photophysics, Conformational Fluctuations, Binding Dynamics; 5.6 Picoseconds to Nanoseconds: Rotational Diffusion and Fluorescence Antibunching; 5.6.1 Antibunching; 5.6.2 Rotational Diffusion; 5.7 Fluorescence Lifetime Correlation Spectroscopy; 5.8 Conclusion; References; 6 Excited State Energy Transfer; 6.1 Introduction; 6.2 Theory of (F€orster) Energy Transfer; 6.2.1 Mechanism and Mathematical Formalism of FRET6.2.2 Measuring FRET Efficiencies Through Excited-State Lifetimes6.2.3 Spin Rules for FRET; 6.2.4 Homo-FRET and FRET-Induced Depolarization; 6.3 Experimental Approach for Single-Pair FRET-Experiments; 6.3.1 Single-Laser Excitation; 6.3.2 Alternating-Laser Excitation (ALEX); 6.4 Examples and Applications of FRET; 6.4.1 FRET Processes in Bulk Experiments; 6.4.1.1 FRET-Based Molecular Biosensors; 6.4.1.2 Energy Hopping and Trapping in Chromophore-Substituted Polyphenylene Dendrimers; 6.4.2 Single-Molecule Observation of FRET; 6.4.2.1 Light-Harvesting Systems: Phycobilisomes and Allophycocyanins6.4.2.2 Hairpin Ribozyme Dynamics and Activity6.4.2.3 Protein (Un)folding and Dynamics; References; 7 Photoinduced Electron Transfer (PET) Reactions; 7.1 Fluorescence Quenching by PET; 7.2 Single-Molecule Fluorescence Spectroscopy to Study PET; 7.3 Single-Molecule Sensitive Fluorescence Sensors Based on PET; 7.4 PET Reporter System; 7.5 Monitoring Conformational Dynamics and Protein Folding by PET; 7.6 Biological and Diagnostic Applications; References; 8 Super-Resolution Fluorescence Imaging; 8.1 Diffraction Barrier of Optical Microscopy8.2 Multi-Photon and Structured Illumination MicroscopyProviding much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging.Examples show how each technique can help in obtaining detailed and refineFluorescence spectroscopyHandbooks, manuals, etcFluorescence microscopyHandbooks, manuals, etcFluorescence spectroscopyFluorescence microscopy543.56Sauer Markus975565Hofkens Johan521656Enderlein J(Jorg)521657MiAaPQMiAaPQMiAaPQBOOK9910133642003321Handbook of fluorescence spectroscopy and imaging2221391UNINA