Animal cell culture : principles and practice / / Shalini Mani, Manisha Singh, and Anil Kumar
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| Autore: |
Mani Shalini
|
| Titolo: |
Animal cell culture : principles and practice / / Shalini Mani, Manisha Singh, and Anil Kumar
|
| Pubblicazione: | Cham, Switzerland : , : Springer, , [2023] |
| ©2023 | |
| Edizione: | 1st ed. 2023. |
| Descrizione fisica: | 1 online resource (332 pages) |
| Disciplina: | 660.6 |
| Soggetto topico: | Cell culture |
| Laboratory manuals | |
| Persona (resp. second.): | SinghManisha |
| Anil Kumar | |
| Note generali: | Includes index. |
| Nota di contenuto: | 1. Overview to animal cell culture -- 2. Cell culture laboratory -- 3. Good lab practices and bio-safety measures -- 4. Managing sterility in the lab -- 5. Media and buffer preparation for cell culture -- 6. Properties of cultured cells and selection of culture media -- 7. Selection and maintenance of cultured cells -- 8. Inoculation and passaging of adherent cells -- 9. Counting of cells -- 10. Cryopreservation of cell lines -- 11. Resuscitation of frozen cell line -- 12. Isolation and culturing of cells from different tissues -- 13. Differentiation, transformation and immortalization of cells -- 14. Co-culture techniques -- 15. 3d cell culture techniques -- 16. Stem cell culture techniques -- 17. Identification and removal of biological contamination in the media and cell suspensions -- 18. Analysis of cell growth kinetics in suspension and adherent types cell line -- 19. Applications of animal cell culture based assays -- 20. Ethical issues in animal cell culture -- 21. Common troubleshooting methods in cell culture techniques. |
| Sommario/riassunto: | This introductory guide provides novice researchers and lab students with a thorough step-by-step approach to standard animal cell culture techniques. Coverage includes lab safety and best practices, sterility management, preparation, ethical considerations, and troubleshooting for common pain points. This is an up-to-date, indispensable handbook for early-career researchers and students, as well as established scientists in biotechnology, cell and developmental biology, pharmaceutical toxicology, cytogenetics, and more. . |
| Titolo autorizzato: | Animal cell culture |
| ISBN: | 3-031-19485-3 |
| Formato: | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione: | Inglese |
| Record Nr.: | 9910647770903321 |
| Lo trovi qui: | Univ. Federico II |
| Opac: | Controlla la disponibilità qui |
Serie:
Techniques in Life Science and Biomedicine for the Non-Expert, . 2367-1122