LEADER 03931nam 22007455 450 001 9910647770903321 005 20251008140519.0 010 $a3-031-19485-3 024 7 $a10.1007/978-3-031-19485-6 035 $a(MiAaPQ)EBC7192197 035 $a(Au-PeEL)EBL7192197 035 $a(CKB)26094867300041 035 $a(DE-He213)978-3-031-19485-6 035 $a(PPN)267817525 035 $a(EXLCZ)9926094867300041 100 $a20230131d2023 u| 0 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aAnimal Cell Culture: Principles and Practice /$fby Shalini Mani, Manisha Singh, Anil Kumar 205 $a1st ed. 2023. 210 1$aCham :$cSpringer International Publishing :$cImprint: Springer,$d2023. 215 $a1 online resource (332 pages) 225 1 $aTechniques in Life Science and Biomedicine for the Non-Expert,$x2367-1122 300 $aIncludes index. 311 08$aPrint version: Mani, Shalini Animal Cell Culture: Principles and Practice Cham : Springer International Publishing AG,c2023 9783031194849 327 $a1. Overview to animal cell culture -- 2. Cell culture laboratory -- 3. Good lab practices and bio-safety measures -- 4. Managing sterility in the lab -- 5. Media and buffer preparation for cell culture -- 6. Properties of cultured cells and selection of culture media -- 7. Selection and maintenance of cultured cells -- 8. Inoculation and passaging of adherent cells -- 9. Counting of cells -- 10. Cryopreservation of cell lines -- 11. Resuscitation of frozen cell line -- 12. Isolation and culturing of cells from different tissues -- 13. Differentiation, transformation and immortalization of cells -- 14. Co-culture techniques -- 15. 3d cell culture techniques -- 16. Stem cell culture techniques -- 17. Identification and removal of biological contamination in the media and cell suspensions -- 18. Analysis of cell growth kinetics in suspension and adherent types cell line -- 19. Applications of animal cell culture based assays -- 20. Ethical issues in animal cell culture -- 21. Common troubleshooting methods in cell culture techniques. 330 $aThis introductory guide provides novice researchers and lab students with a thorough step-by-step approach to standard animal cell culture techniques. Coverage includes lab safety and best practices, sterility management, preparation, ethical considerations, and troubleshooting for common pain points. This is an up-to-date, indispensable handbook for early-career researchers and students, as well as established scientists in biotechnology, cell and developmental biology, pharmaceutical toxicology, cytogenetics, and more. . 410 0$aTechniques in Life Science and Biomedicine for the Non-Expert,$x2367-1122 606 $aCytology$xTechnique 606 $aMicrobiology$xTechnique 606 $aBiotechnology 606 $aCytology 606 $aBiology$xTechnique 606 $aExperimental immunology 606 $aCytological Techniques 606 $aMicrobiology Techniques 606 $aBiotechnology 606 $aCell Biology 606 $aImmunological Techniques 615 0$aCytology$xTechnique. 615 0$aMicrobiology$xTechnique. 615 0$aBiotechnology. 615 0$aCytology. 615 0$aBiology$xTechnique. 615 0$aExperimental immunology. 615 14$aCytological Techniques. 615 24$aMicrobiology Techniques. 615 24$aBiotechnology. 615 24$aCell Biology. 615 24$aImmunological Techniques. 676 $a660.6 676 $a571.638078 700 $aMani$b Shalini$01280183 702 $aSingh$b Manisha 702 $aAnil Kumar 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910647770903321 996 $aAnimal cell culture$93364360 997 $aUNINA