03931nam 22007455 450 991064777090332120251008140519.03-031-19485-310.1007/978-3-031-19485-6(MiAaPQ)EBC7192197(Au-PeEL)EBL7192197(CKB)26094867300041(DE-He213)978-3-031-19485-6(PPN)267817525(EXLCZ)992609486730004120230131d2023 u| 0engurcnu||||||||txtrdacontentcrdamediacrrdacarrierAnimal Cell Culture: Principles and Practice /by Shalini Mani, Manisha Singh, Anil Kumar1st ed. 2023.Cham :Springer International Publishing :Imprint: Springer,2023.1 online resource (332 pages)Techniques in Life Science and Biomedicine for the Non-Expert,2367-1122Includes index.Print version: Mani, Shalini Animal Cell Culture: Principles and Practice Cham : Springer International Publishing AG,c2023 9783031194849 1. Overview to animal cell culture -- 2. Cell culture laboratory -- 3. Good lab practices and bio-safety measures -- 4. Managing sterility in the lab -- 5. Media and buffer preparation for cell culture -- 6. Properties of cultured cells and selection of culture media -- 7. Selection and maintenance of cultured cells -- 8. Inoculation and passaging of adherent cells -- 9. Counting of cells -- 10. Cryopreservation of cell lines -- 11. Resuscitation of frozen cell line -- 12. Isolation and culturing of cells from different tissues -- 13. Differentiation, transformation and immortalization of cells -- 14. Co-culture techniques -- 15. 3d cell culture techniques -- 16. Stem cell culture techniques -- 17. Identification and removal of biological contamination in the media and cell suspensions -- 18. Analysis of cell growth kinetics in suspension and adherent types cell line -- 19. Applications of animal cell culture based assays -- 20. Ethical issues in animal cell culture -- 21. Common troubleshooting methods in cell culture techniques.This introductory guide provides novice researchers and lab students with a thorough step-by-step approach to standard animal cell culture techniques. Coverage includes lab safety and best practices, sterility management, preparation, ethical considerations, and troubleshooting for common pain points. This is an up-to-date, indispensable handbook for early-career researchers and students, as well as established scientists in biotechnology, cell and developmental biology, pharmaceutical toxicology, cytogenetics, and more. .Techniques in Life Science and Biomedicine for the Non-Expert,2367-1122CytologyTechniqueMicrobiologyTechniqueBiotechnologyCytologyBiologyTechniqueExperimental immunologyCytological TechniquesMicrobiology TechniquesBiotechnologyCell BiologyImmunological TechniquesCytologyTechnique.MicrobiologyTechnique.Biotechnology.Cytology.BiologyTechnique.Experimental immunology.Cytological Techniques.Microbiology Techniques.Biotechnology.Cell Biology.Immunological Techniques.660.6571.638078Mani Shalini1280183Singh ManishaAnil KumarMiAaPQMiAaPQMiAaPQBOOK9910647770903321Animal cell culture3364360UNINA