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1. |
Record Nr. |
UNIORUON00400624 |
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Autore |
BULEI, Ion |
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Titolo |
Atunci cind veacul se nastea... : Lumea romaneasca 1900-1908 / Ion Bulei |
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Pubbl/distr/stampa |
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Bucuresti, : Editura Eminescu, 1990 |
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Descrizione fisica |
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Disciplina |
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Soggetti |
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Romania - Storia - Sec. 20 |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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2. |
Record Nr. |
UNINA9910647770903321 |
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Autore |
Mani Shalini |
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Titolo |
Animal Cell Culture: Principles and Practice / / by Shalini Mani, Manisha Singh, Anil Kumar |
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Pubbl/distr/stampa |
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Cham : , : Springer International Publishing : , : Imprint : Springer, , 2023 |
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ISBN |
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Edizione |
[1st ed. 2023.] |
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Descrizione fisica |
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1 online resource (332 pages) |
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Collana |
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Techniques in Life Science and Biomedicine for the Non-Expert, , 2367-1122 |
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Disciplina |
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Soggetti |
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Cytology - Technique |
Microbiology - Technique |
Biotechnology |
Cytology |
Biology - Technique |
Experimental immunology |
Cytological Techniques |
Microbiology Techniques |
Cell Biology |
Immunological Techniques |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Nota di contenuto |
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1. Overview to animal cell culture -- 2. Cell culture laboratory -- 3. Good lab practices and bio-safety measures -- 4. Managing sterility in the lab -- 5. Media and buffer preparation for cell culture -- 6. Properties of cultured cells and selection of culture media -- 7. Selection and maintenance of cultured cells -- 8. Inoculation and passaging of adherent cells -- 9. Counting of cells -- 10. Cryopreservation of cell lines -- 11. Resuscitation of frozen cell line -- 12. Isolation and culturing of cells from different tissues -- 13. Differentiation, transformation and immortalization of cells -- 14. Co-culture techniques -- 15. 3d cell culture techniques -- 16. Stem cell culture techniques -- 17. Identification and removal of biological contamination in the media and cell suspensions -- 18. Analysis of cell growth kinetics in suspension and adherent types cell line -- 19. Applications of animal cell culture based assays -- 20. Ethical issues in animal cell culture -- 21. Common troubleshooting methods in cell culture techniques. |
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Sommario/riassunto |
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This introductory guide provides novice researchers and lab students with a thorough step-by-step approach to standard animal cell culture techniques. Coverage includes lab safety and best practices, sterility management, preparation, ethical considerations, and troubleshooting for common pain points. This is an up-to-date, indispensable handbook for early-career researchers and students, as well as established scientists in biotechnology, cell and developmental biology, pharmaceutical toxicology, cytogenetics, and more. . |
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