Directed molecular evolution of proteins : or how to improve enzymes for biocatalysis

• Pubblicazione: [Place of publication not identified], : Wiley VCH, 2002
• Descrizione fisica: 1 online resource (359 pages)
• Disciplina: 660.6/34
• Soggetto topico: Enzymes - Biotechnology; Biocatalysis - Evolution; Proteins - Chemical modification; Proteins; Molecular evolution; Combinatorial chemistry; Directed Molecular Evolution; Protein Engineering; Genetic Engineering; Genetic Techniques; Investigative Techniques; Analytical, Diagnostic and Therapeutic Techniques and Equipment; Biomedical Engineering; Health & Biological Sciences
• Soggetto genere / forma: Electronic books.
• Persona (resp. second.): BrakmannSusanne; JohnssonKai
• Note generali: Bibliographic Level Mode of Issuance: Monograph
• Sommario/riassunto: Natural selection created optimal catalysts. However, optimal performance of enzyme catalysis does not necessarily refer to maximum reaction rate. Rather, it may be a compromise between specificity, rate, stability, and other chemical constraints that makes enzymes capable of catalyzing reactions under mild conditions and with high substrate specificity, accompanied by high regio- and enantioselectivity. The book presented here focuses on the directed evolution of proteins, which has established itself as a powerful method for designing enzymes showing new substrate specificities. It includes a comprehensive repertoire of techniques for producing combinatorial enzyme libraries, while the functional gene expression in a suitable host helps in selecting the appropriate structure, making fast screening a necessity.
• Titolo autorizzato: Directed molecular evolution of proteins : or how to improve enzymes for biocatalysis
• ISBN: 1-280-55837-7; 9786610558377; 3-527-60064-7
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Lingua di pubblicazione: Inglese
• Record Nr.: 9910146244503321
• Lo trovi qui: Univ. Federico II