03417oam 2200805zu 450 991014624450332120210721053955.01-280-55837-797866105583773-527-60064-7(CKB)1000000000019305(SSID)ssj0000294044(PQKBManifestationID)11222947(PQKBTitleCode)TC0000294044(PQKBWorkID)10303276(PQKB)10668017(MiAaPQ)EBC4956603(Au-PeEL)EBL4956603(CaONFJC)MIL55837(OCoLC)1024267738(EXLCZ)99100000000001930520160829d2002 uy engurcnu||||||||txtccrDirected molecular evolution of proteins : or how to improve enzymes for biocatalysis[Place of publication not identified]Wiley VCH20021 online resource (359 pages)Bibliographic Level Mode of Issuance: Monograph3-527-30423-1 Natural selection created optimal catalysts. However, optimal performance of enzyme catalysis does not necessarily refer to maximum reaction rate. Rather, it may be a compromise between specificity, rate, stability, and other chemical constraints that makes enzymes capable of catalyzing reactions under mild conditions and with high substrate specificity, accompanied by high regio- and enantioselectivity. The book presented here focuses on the directed evolution of proteins, which has established itself as a powerful method for designing enzymes showing new substrate specificities. It includes a comprehensive repertoire of techniques for producing combinatorial enzyme libraries, while the functional gene expression in a suitable host helps in selecting the appropriate structure, making fast screening a necessity.EnzymesBiotechnologyBiocatalysisEvolutionProteinsChemical modificationProteinsMolecular evolutionCombinatorial chemistryDirected Molecular EvolutionProtein EngineeringGenetic EngineeringGenetic TechniquesInvestigative TechniquesAnalytical, Diagnostic and Therapeutic Techniques and EquipmentBiomedical EngineeringHILCCHealth & Biological SciencesHILCCElectronic books.EnzymesBiotechnologyBiocatalysisEvolutionProteinsChemical modificationProteinsMolecular evolutionCombinatorial chemistryDirected Molecular EvolutionProtein EngineeringGenetic EngineeringGenetic TechniquesInvestigative TechniquesAnalytical, Diagnostic and Therapeutic Techniques and EquipmentBiomedical EngineeringHealth & Biological Sciences660.6/34Brakmann SusanneJohnsson KaiPQKBBOOK9910146244503321Directed molecular evolution of proteins : or how to improve enzymes for biocatalysis2154822UNINA