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Record Nr. |
UNINA9910146244503321 |
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Titolo |
Directed molecular evolution of proteins : or how to improve enzymes for biocatalysis |
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Pubbl/distr/stampa |
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[Place of publication not identified], : Wiley VCH, 2002 |
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ISBN |
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1-280-55837-7 |
9786610558377 |
3-527-60064-7 |
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Descrizione fisica |
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1 online resource (359 pages) |
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Disciplina |
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Soggetti |
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Enzymes - Biotechnology |
Biocatalysis - Evolution |
Proteins - Chemical modification |
Proteins |
Molecular evolution |
Combinatorial chemistry |
Directed Molecular Evolution |
Protein Engineering |
Genetic Engineering |
Genetic Techniques |
Investigative Techniques |
Analytical, Diagnostic and Therapeutic Techniques and Equipment |
Biomedical Engineering |
Health & Biological Sciences |
Electronic books. |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Bibliographic Level Mode of Issuance: Monograph |
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Sommario/riassunto |
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Natural selection created optimal catalysts. However, optimal performance of enzyme catalysis does not necessarily refer to maximum reaction rate. Rather, it may be a compromise between specificity, rate, stability, and other chemical constraints that makes |
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enzymes capable of catalyzing reactions under mild conditions and with high substrate specificity, accompanied by high regio- and enantioselectivity. The book presented here focuses on the directed evolution of proteins, which has established itself as a powerful method for designing enzymes showing new substrate specificities. It includes a comprehensive repertoire of techniques for producing combinatorial enzyme libraries, while the functional gene expression in a suitable host helps in selecting the appropriate structure, making fast screening a necessity. |
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