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Automatische genetische Analytik [[electronic resource] /] / Günter Mertes ... <et al.>
| Automatische genetische Analytik [[electronic resource] /] / Günter Mertes ... <et al.> |
| Pubbl/distr/stampa | Weinheim ; ; New York, : Wiley-VCH, c1997 |
| Descrizione fisica | 1 online resource (252 p.) |
| Disciplina |
572.86
576.5 |
| Altri autori (Persone) | MertesG. nter |
| Soggetto topico |
Genetics
DNA |
| ISBN |
1-282-02183-4
9786612021831 3-527-62436-8 3-527-62437-6 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | ger |
| Nota di contenuto |
Automatische genetische Analytik; Copyright Page; Vorwort; Geleitwort; Inhalt; 1 Bedarf und Konzept einer integrierten automatischen DNA-Analyse; 1.1 Was hat die DNA-Analyse so populär gemacht?; 1.2 Methodische Quantensprunge machten die DNA,, reif"" für die Routineanalyse; 1.3 Was bedeutet integrierte automatische genetische Analyse?; 1.3.1 Teilbereiche der molekularen DNA-Analyse; 1.3.2 Das Konzept der Integration; 2 Die DNA-Präparation für die automatische DNA- Analyse; 2.1 Einführung; 2.2 Vektoren für die DNA-Präparation; 2.3 DNA-Präparation durch PCR; 2.4 Methoden zur Plasmidpraparation
2.4.1 Alkalische Lyse2.4.2 Boiling-Methode; 2.4.3 Aufreinigung der Plasmid-DNA über Säulen; 2.5 ,,Solid-Phase""-DNA-Präparation; 2.6 Molekularbiologische Workstation; 2.7 Auswirkungen der DNA-Template-Qualität auf die automatische DNA-Sequenzierung; 2.8 Literatur; 3 Die PCR als Grundlage in der molekularen DNA- Analyse; 3.1 PCR - das Grundprinzip; 3.2 Automatisierung der PCR; 3.3 Optimierung von PCR-Reaktionen; 3.3.1 Reaktionsparameter; 3.3.2 Optimierungsstrategien; 3.4 Optimierung der Amplifikationspräzision; 3.5 Spezielle PCR-Verfahren; 3.5.1 Touchdown-PCR; 3.5.2 Nested-PCR 3.5.3 Hot-Start-Technik3.6 Thermostabile Enzyme; 3.6.1 Taq-DNA-Polymerase; 3.6.2 rTth -DNA-Polymerase; 3.6.3 VentTM DNA-Polymerase; 3.6.4 Pfu-DNA-Polymerase; 3.6.5 UITmaTM DNA-Polymerase; 3.7 PCR und Kontaminationen; 3.8 Analyse der Amplifikationsprodukte; 3.8.1 Gelelektrophorese; 3.8.2 High Performance Liquid Chromatographie (HPLC); 3.8.3 Kapillar-Elektrophorese (CE); 3.8.4 TaqManTM-Assay zur Analyse von PCR-Produkten; 3.9 Literatur; 4 Die DNA-Synthese als grundlegendes Werkzeug in der molekularen DNA-Analyse; 4.1 Entwicklung der DNA-Synthese; 4.2 Chemische Grundlagen der DNA-Synthese 4.3 Chemischer Ablauf der DNA-Synthese4.3.1 Detritylierung; 4.3.2 Monomeraddition; 4.3.3 Capping; 4.3.4 Oxidation; 4.4 Automatisierung der DNA-Synthese; 4.5 Optimierung der DNA-Synthese; 4.6 Aufarbeitung von Oligonukleotiden; 4.6.1 Gelelektrophorese; 4.6.2 High Performance Liquid Chromatographie (HPLC); 4.6.3 Kapillar-Elektrophorese; 4.7 Mögliche Konsequenzen der Verwendung nichtgereinigter Oligonukleotide auf spätere Anwendungen; 4.8 Markierung von Oligonukleotiden; 4.8.1 Biotin-Markierung; 4.8.2 Phosphorylierung; 4.8.3 Fluoreszenzmarkierung; 4.9 Anwendungen von Oligonukleotiden 4.10 Literatur5 DNA-Sequenzanalyse; 5.1 Einleitung; 5.2 Sequenzier-Techniken; 5.2.1 Maxam-Gilbert-Sequenzierung; 5.2.2 Sequenzierung nach Sanger; 5.2.3 ,,Cycle-Sequenzierung""; 5.2.4 Multiplex-Sequenzierung; 5.3 Templates; 5.3.1 Phagen und Phagemide; 5.3.2 Plasmide und Cosmide; 5.3.3 PCR-Produkte; 5.3.4 Magnetic Beads; 5.4 Markierungs-Methoden; 5.4.1 Sequenzierung mit markierten Primern; 5.4.2 Sequenzierung mit markierten Desoxynukleotiden; 5.4.3 Sequenzierung mit markierten Didesoxynukleotiden (DyeTerminatoren); 5.4.4 Nachträgliche Sequenz-Markierung; 5.5 Der Weg zur vollständigen Sequenz 5.5.1 Geringer Aufwand für die Probenvorbereitung |
| Record Nr. | UNINA-9910841521403321 |
| Weinheim ; ; New York, : Wiley-VCH, c1997 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Autosomal Dominant Polycystic Kidney Disease : : Seminar, Vimercate, June 1994 / / editors, A. Sessa, P. Serbelloni, S. Milani, F. Conte
| Autosomal Dominant Polycystic Kidney Disease : : Seminar, Vimercate, June 1994 / / editors, A. Sessa, P. Serbelloni, S. Milani, F. Conte |
| Autore | Sessa A |
| Pubbl/distr/stampa | Basel : , : S. Karger, , 1995 |
| Descrizione fisica | 1 online resource (X + 192 pages) : : 47 figures, 39 tables |
| Disciplina | 616.6/1 |
| Altri autori (Persone) | SessaA |
| Collana | Contributions to Nephrology |
| Soggetto topico |
Nephrology
Genetics Dialysis Molecular Biology Pathology Urology |
| ISBN | 3-318-03379-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910156329103321 |
Sessa A
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| Basel : , : S. Karger, , 1995 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Babies by Design : The Ethics of Genetic Choice / / Ronald M. Green
| Babies by Design : The Ethics of Genetic Choice / / Ronald M. Green |
| Autore | Green Ronald M. |
| Pubbl/distr/stampa | New Haven, CT : , : Yale University Press, , [2008] |
| Descrizione fisica | 1 online resource (288 p.) |
| Disciplina | 174.2 |
| Soggetto topico |
Medical genetics - Moral and ethical aspects
Genetic engineering - Moral and ethical aspects Human reproductive technology - Moral and ethical aspects Prenatal diagnosis - Moral and ethical aspects Genetic disorders in children - Moral and ethical aspects - Prevention Genetic counseling - Moral and ethical aspects Genetic Techniques Morals Religion Genetics Medicine Psychology, Social Humanities Health Occupations Investigative Techniques Biology Biological Science Disciplines Analytical, Diagnostic and Therapeutic Techniques and Equipment Disciplines and Occupations Behavior and Behavior Mechanisms Natural Science Disciplines Psychiatry and Psychology Genetics, Medical Ethics Genetic Engineering Religion and Medicine Health & Biological Sciences Pathology |
| Soggetto genere / forma | Electronic books. |
| ISBN | 0-300-13857-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Frontmatter -- Contents -- Introduction -- 1. Creating the Superathlete -- 2. How Will We Do It? -- 3. Drawing Lines -- 4. Challenges and Risks -- 5. Parents: Guardians or Gardeners? -- 6. Will We Create a "Genobility"? -- 7. Playing God -- 8. The Choices Ahead -- Notes -- Glossary -- Acknowledgments -- Index |
| Record Nr. | UNINA-9910452849403321 |
|
Green Ronald M.
|
||
| New Haven, CT : , : Yale University Press, , [2008] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Babies by Design : The Ethics of Genetic Choice / / Ronald M. Green
| Babies by Design : The Ethics of Genetic Choice / / Ronald M. Green |
| Autore | Green Ronald Michael |
| Pubbl/distr/stampa | New Haven, CT : , : Yale University Press, , [2008] |
| Descrizione fisica | 1 online resource (288 p.) |
| Disciplina | 174.2 |
| Soggetto topico |
Medical genetics - Moral and ethical aspects
Genetic engineering - Moral and ethical aspects Human reproductive technology - Moral and ethical aspects Prenatal diagnosis - Moral and ethical aspects Genetic disorders in children - Moral and ethical aspects - Prevention Genetic counseling - Moral and ethical aspects Genetic Techniques Morals Religion Genetics Medicine Psychology, Social Humanities Health Occupations Investigative Techniques Biology Biological Science Disciplines Behavior and Behavior Mechanisms Natural Science Disciplines Genetics, Medical Ethics Genetic Engineering Religion and Medicine Health & Biological Sciences Pathology |
| Soggetto genere / forma | Electronic books. |
| ISBN | 0-300-13857-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Frontmatter -- Contents -- Introduction -- 1. Creating the Superathlete -- 2. How Will We Do It? -- 3. Drawing Lines -- 4. Challenges and Risks -- 5. Parents: Guardians or Gardeners? -- 6. Will We Create a "Genobility"? -- 7. Playing God -- 8. The Choices Ahead -- Notes -- Glossary -- Acknowledgments -- Index |
| Record Nr. | UNINA-9910779244203321 |
|
Green Ronald Michael
|
||
| New Haven, CT : , : Yale University Press, , [2008] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Babies by Design : The Ethics of Genetic Choice / / Ronald M. Green
| Babies by Design : The Ethics of Genetic Choice / / Ronald M. Green |
| Autore | Green Ronald Michael |
| Pubbl/distr/stampa | New Haven, CT : , : Yale University Press, , [2008] |
| Descrizione fisica | 1 online resource (288 p.) |
| Disciplina | 174.2 |
| Soggetto topico |
Medical genetics - Moral and ethical aspects
Genetic engineering - Moral and ethical aspects Human reproductive technology - Moral and ethical aspects Prenatal diagnosis - Moral and ethical aspects Genetic disorders in children - Moral and ethical aspects - Prevention Genetic counseling - Moral and ethical aspects Genetic Techniques Morals Religion Genetics Medicine Psychology, Social Humanities Health Occupations Investigative Techniques Biology Biological Science Disciplines Behavior and Behavior Mechanisms Natural Science Disciplines Genetics, Medical Ethics Genetic Engineering Religion and Medicine Health & Biological Sciences Pathology |
| Soggetto genere / forma | Electronic books. |
| ISBN | 0-300-13857-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Frontmatter -- Contents -- Introduction -- 1. Creating the Superathlete -- 2. How Will We Do It? -- 3. Drawing Lines -- 4. Challenges and Risks -- 5. Parents: Guardians or Gardeners? -- 6. Will We Create a "Genobility"? -- 7. Playing God -- 8. The Choices Ahead -- Notes -- Glossary -- Acknowledgments -- Index |
| Record Nr. | UNINA-9910808253503321 |
|
Green Ronald Michael
|
||
| New Haven, CT : , : Yale University Press, , [2008] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
BAG, journal of basic and applied genetics
| BAG, journal of basic and applied genetics |
| Pubbl/distr/stampa | Buenos Aires, Argentina : , : Sociedad Argentina de Genética |
| Descrizione fisica | 1 online resource |
| Soggetto topico | Genetics |
| Soggetto genere / forma | Periodicals. |
| Soggetto non controllato | Genetics |
| Formato | Materiale a stampa |
| Livello bibliografico | Periodico |
| Lingua di pubblicazione | eng |
| Altri titoli varianti | Journal of basic and applied genetics |
| Record Nr. | UNISA-996343849503316 |
| Buenos Aires, Argentina : , : Sociedad Argentina de Genética | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. di Salerno | ||
| ||
BAG, journal of basic and applied genetics
| BAG, journal of basic and applied genetics |
| Pubbl/distr/stampa | Buenos Aires, Argentina : , : Sociedad Argentina de Genética |
| Descrizione fisica | 1 online resource |
| Soggetto topico | Genetics |
| Soggetto genere / forma | Periodicals. |
| Soggetto non controllato | Genetics |
| Formato | Materiale a stampa |
| Livello bibliografico | Periodico |
| Lingua di pubblicazione | eng |
| Altri titoli varianti | Journal of basic and applied genetics |
| Record Nr. | UNINA-9910130883803321 |
| Buenos Aires, Argentina : , : Sociedad Argentina de Genética | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Basic Research and Clinical Aspects of Pseudomonas Aeruginosa : : International Symposium on Pseudomonas Aeruginosa, Tübingen, June 1986 / / editors, G. Döring, I.A. Holder, K. Botzenhart
| Basic Research and Clinical Aspects of Pseudomonas Aeruginosa : : International Symposium on Pseudomonas Aeruginosa, Tübingen, June 1986 / / editors, G. Döring, I.A. Holder, K. Botzenhart |
| Autore | Döring G |
| Pubbl/distr/stampa | Basel : , : S. Karger, , 1987 |
| Descrizione fisica | 1 online resource (X + 314 pages) : : 56 figures, 66 tables |
| Disciplina |
615.5/8 s
617/.22 |
| Altri autori (Persone) |
DöringG
HolderIan Alan BotzenhartK |
| Soggetto topico |
Pharmacology
Infectious Diseases Pharmaceutics Bacteriology Chemotherapy Epidemiology Genetics Microbiology |
| ISBN | 3-318-03214-X |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910156328903321 |
|
Döring G
|
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| Basel : , : S. Karger, , 1987 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Behavior genetics
| Behavior genetics |
| Pubbl/distr/stampa | New York, N.Y., : Kluwer Academic/Plenum Publishers |
| Descrizione fisica | 1 online resource |
| Disciplina | 155.7 |
| Soggetto topico |
Behavior genetics
Behavior evolution Genetic psychology Genetics, Behavioral Genetics Génétique du comportement Évolution du comportement Psychologie génétique Genetics & Genomics Human Behavior |
| Soggetto genere / forma | Periodicals. |
| ISSN | 1573-3297 |
| Formato | Materiale a stampa |
| Livello bibliografico | Periodico |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910131799903321 |
| New York, N.Y., : Kluwer Academic/Plenum Publishers | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Behavior genetics
| Behavior genetics |
| Pubbl/distr/stampa | New York, N.Y., : Kluwer Academic/Plenum Publishers |
| Descrizione fisica | 1 online resource |
| Disciplina | 155.7 |
| Soggetto topico |
Behavior genetics
Behavior evolution Genetic psychology Genetics, Behavioral Genetics Génétique du comportement Évolution du comportement Psychologie génétique Genetics & Genomics Human Behavior |
| Soggetto genere / forma | Periodicals. |
| ISSN | 1573-3297 |
| Formato | Materiale a stampa |
| Livello bibliografico | Periodico |
| Lingua di pubblicazione | eng |
| Record Nr. | UNISA-996203650403316 |
| New York, N.Y., : Kluwer Academic/Plenum Publishers | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. di Salerno | ||
| ||
Soggetto topico
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Genetics
(654)
- Biology (84)
- Health & Biological Sciences (62)
- Genomics (54)
- Molecular Biology (48)