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Fluorescence microscopy : from principles to biological applications / / edited by Ulrich Kubitscheck
| Fluorescence microscopy : from principles to biological applications / / edited by Ulrich Kubitscheck |
| Autore | Kubitscheck Ulrich |
| Edizione | [2nd edition.] |
| Pubbl/distr/stampa | [Weinheim, Germany] : , : Wiley-VCH, , 2017 |
| Descrizione fisica | 1 online resource (507 pages) |
| Disciplina | 502.82 |
| Soggetto topico | Fluorescence microscopy |
| ISBN |
3-527-68774-2
1-5231-1530-0 3-527-68772-6 3-527-68773-4 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910270892803321 |
Kubitscheck Ulrich
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| [Weinheim, Germany] : , : Wiley-VCH, , 2017 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Fluorescence microscopy : from principles to biological applications / / edited by Ulrich Kubitscheck
| Fluorescence microscopy : from principles to biological applications / / edited by Ulrich Kubitscheck |
| Autore | Kubitscheck Ulrich |
| Edizione | [2nd edition.] |
| Pubbl/distr/stampa | [Weinheim, Germany] : , : Wiley-VCH, , 2017 |
| Descrizione fisica | 1 online resource (507 pages) |
| Disciplina | 502.82 |
| Soggetto topico | Fluorescence microscopy |
| ISBN |
3-527-68774-2
1-5231-1530-0 3-527-68772-6 3-527-68773-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910814764303321 |
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Kubitscheck Ulrich
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||
| [Weinheim, Germany] : , : Wiley-VCH, , 2017 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Fluorescence microscopy : super-resolution and other novel techniques / edited by Anda Cornea and P. Michael Conn
| Fluorescence microscopy : super-resolution and other novel techniques / edited by Anda Cornea and P. Michael Conn |
| Pubbl/distr/stampa | Burlington, : Elsevier Science, 2014 |
| Descrizione fisica | Testo elettronico (PDF) (249 p.) |
| Disciplina | 502.82 |
| Soggetto topico | Microscopia di fluorescenza |
| Formato | Risorse elettroniche  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNISA-996459752303316 |
| Burlington, : Elsevier Science, 2014 | ||
| Risorse elettroniche | ||
| Lo trovi qui: Univ. di Salerno | ||
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Fluorescence microscopy [[electronic resource] ] : from principles to biological applications / / edited by Ulrich Kubitscheck
| Fluorescence microscopy [[electronic resource] ] : from principles to biological applications / / edited by Ulrich Kubitscheck |
| Pubbl/distr/stampa | Weinheim [Germany], : Wiley-Blackwell, 2013 |
| Descrizione fisica | 1 online resource (432 p.) |
| Disciplina | 502.82 |
| Altri autori (Persone) | KubitscheckUlrich |
| Soggetto topico |
Fluorescence microscopy
Microscopy |
| ISBN |
3-527-67159-5
3-527-67161-7 1-299-46465-3 3-527-67160-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Fluorescence Microscopy; Contents; Preface; List of Contributors; 1 Introduction to Optics and Photophysics; 1.1 Interference: Light as a Wave; 1.2 Two Effects of Interference: Diffraction and Refraction; 1.3 Optical Elements; 1.3.1 Lenses; 1.3.2 Metallic Mirror; 1.3.3 Dielectric Mirror; 1.3.4 Pinholes; 1.3.5 Filters; 1.3.6 Chromatic Reflectors; 1.4 The Far-Field, Near-Field, and Evanescent Waves; 1.5 Optical Aberrations; 1.6 Physical Background of Fluorescence; 1.7 Photons, Poisson Statistics, and AntiBunching; References; 2 Principles of Light Microscopy; 2.1 Introduction
2.2 Construction of Light Microscopes2.2.1 Components of Light Microscopes; 2.2.2 Imaging Path; 2.2.3 Magnification; 2.2.4 Angular and Numerical Aperture; 2.2.5 Field of View; 2.2.6 Illumination Beam Path; 2.3 Wave Optics and Resolution; 2.3.1 Wave Optical Description of the Imaging Process; 2.3.2 The Airy Function; 2.3.3 Point Spread Function and Optical Transfer Function; 2.3.4 Lateral and Axial Resolution; 2.3.5 Magnification and Resolution; 2.3.6 Depth of Field and Depth of Focus; 2.3.7 Over- and Under Sampling; 2.4 Apertures, Pupils, and Telecentricity; 2.5 Microscope Objectives 2.5.1 Objective Lens Design2.5.2 Light Collection Efficiency and Image Brightness; 2.5.3 Objective Lens Classes; 2.5.4 Immersion Media; 2.5.5 Special Applications; 2.6 Contrast; 2.6.1 Dark Field; 2.6.2 Phase Contrast; 2.6.3 Interference Contrast; 2.6.4 Advanced Topic: Differential Interference Contrast; 2.7 Summary; Acknowledgments; References; 3 Fluorescence Microscopy; 3.1 Features of Fluorescence Microscopy; 3.1.1 Image Contrast; 3.1.2 Specificity of Fluorescence Labeling; 3.1.3 Sensitivity of Detection; 3.2 A Fluorescence Microscope; 3.2.1 Principle of Operation 3.2.2 Sources of Exciting Light3.2.3 Optical Filters in a Fluorescence Microscope; 3.2.4 Electronic Filters; 3.2.5 Photodetectors for Fluorescence Microscopy; 3.2.6 CCD-Charge-Coupled Device; 3.2.7 Intensified CCD (ICCD); 3.2.8 Electron-Multiplying Charge-Coupled Device (EMCCD); 3.2.9 CMOS; 3.2.10 Scientific CMOS (sCMOS); 3.2.11 Features of CCD and CMOS Cameras; 3.2.12 Choosing a Digital Camera for Fluorescence Microscopy; 3.2.13 Photomultiplier Tube (PMT); 3.2.14 Avalanche Photodiode (APD); 3.3 Types of Noise in a Digital Microscopy Image; 3.4 Quantitative Fluorescence Microscopy 3.4.1 Measurements of Fluorescence Intensity and Concentration of the Labeled Target3.4.2 Ratiometric Measurements (Ca++, pH); 3.4.3 Measurements of Dimensions in 3D Fluorescence Microscopy; 3.4.4 Measurements of Exciting Light Intensity; 3.4.5 Technical Tips for Quantitative Fluorescence Microscopy; 3.5 Limitations of Fluorescence Microscopy; 3.5.1 Photobleaching; 3.5.2 Reversible Photobleaching under Oxidizing or Reducing Conditions; 3.5.3 Phototoxicity; 3.5.4 Optical Resolution; 3.5.5 Misrepresentation of Small Objects; 3.6 Current Avenues of Development; References; Further Reading Recommended Internet Resources |
| Record Nr. | UNINA-9910139007403321 |
| Weinheim [Germany], : Wiley-Blackwell, 2013 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Fluorescence microscopy of living cells in culture
| Fluorescence microscopy of living cells in culture |
| Pubbl/distr/stampa | San Diego, : Academic Press, 1989 |
| Descrizione fisica | 2 v. ; 23 cm |
| Disciplina | 502.82 |
| Collana | Methods in cell biology |
| Soggetto non controllato | Microscopia |
| ISBN | 0-12-684754-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Part A.: Fluorescent analogs, labeling cells and basic microscopy / edited by Yu-Li Wang, D. Lansing Taylor Part B.: Quantitative fluorescence microscopy-imaging and spectroscopy / edited by D. Lansing Taylor, Yu-Li Wang |
| Record Nr. | UNINA-990001750990403321 |
| San Diego, : Academic Press, 1989 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Fluorescence spectroscopy and microscopy in biology / / Radek Sachl and Mariana Amaro, editors
| Fluorescence spectroscopy and microscopy in biology / / Radek Sachl and Mariana Amaro, editors |
| Edizione | [1st ed. 2023.] |
| Pubbl/distr/stampa | Cham, Switzerland : , : Springer Nature Switzerland AG, , [2023] |
| Descrizione fisica | 1 online resource (529 pages) |
| Disciplina | 502.82 |
| Collana | Springer Series on Fluorescence, Methods and Applications |
| Soggetto topico |
Fluorescence microscopy
Fluorescence spectroscopy |
| ISBN |
9783031303623
9783031303616 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Part I - Fluorescence Spectroscopy: Basics and Advanced Approaches -- Chapter 1 Choosing the right fluorescent probe -- Chapter 2 Fluorescence kinetics and time-resolved measurement -- Chapter 3 Quantitative Approach to Applications of Electronic Energy Transfer (EET) -- Chapter 4 Single-molecule FRET: principles and analysis -- Chapter 5 Principles of fluorescence correlation and dual-color cross-correlation spectroscopy -- Part II - Fluorescence Microscopy: Basics and Advanced Approaches -- Chapter 6 Introduction to Fluorescence Microscopy -- Chapter 7 Wohland STED and RESOLFT fluorescent nanoscopy -- Chapter 8 Fluorescence Correlation Spectroscopy in Space and Time -- Part C - Applications of Fluorescence Spectroscopy and Microscopy to biological membranes -- Chapter 9 Determination of Biomolecular Oligomerization in the Live Cell Plasma Membrane via Single-molecule Brightness and Co-localization Analysis -- Chapter 10 Quantitative photoactivated localization microscopy of membrane receptor oligomers -- Chapter 11 Diffusion measurements at the nanoscale with STED-FCS -- Chapter 12 Single molecule microscopy methods to study mitochondrial processes -- Chapter 13 Transient state (TRAST) spectroscopy and imaging – exploiting the rich information source of fluorophore dark state transitions in biomolecular and cellular studies -- Chapter 14 The analysis of in-membrane nanoscopic aggregation of lipids and proteins by MC-FRET -- Part D - Applications of Fluorescence Spectroscopy to protein studies -- Chapter 15 Single-molecule fluorescence spectroscopy of intrinsically disordered proteins -- Chapter 16 Insights into the conformational dynamics of potassium channels using homo-FRET approaches -- Chapter 17 Intrinsic fluorescence kinetics in proteins -- Chapter 18 Dynamics and hydration of proteins viewed by fluorescence methods: investigations famor protein engineering and synthetic biology. |
| Record Nr. | UNINA-9910720085403321 |
| Cham, Switzerland : , : Springer Nature Switzerland AG, , [2023] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Force microscopy [[electronic resource] ] : applications in biology and medicine / / edited by Bhanu P. Jena, J.K. Heinrich Hörber
| Force microscopy [[electronic resource] ] : applications in biology and medicine / / edited by Bhanu P. Jena, J.K. Heinrich Hörber |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley-Liss, c2006 |
| Descrizione fisica | 1 online resource (312 p.) |
| Disciplina |
502.82
610.28 |
| Altri autori (Persone) |
JenaBhanu P
HörberJ. K. Heinrich |
| Soggetto topico |
Medical electronics
Scanning force microscopy Scanning probe microscopy Nanotechnology |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-280-50788-8
9786610507887 0-470-00770-2 0-470-00769-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
FORCE MICROSCOPY; CONTENTS; Preface; Contributors; Chapter 1. Porosome: The Universal Secretory Machinery in Cells; Chapter 2. Molecular Mechanism of SNARE-Induced Membrane Fusion; Chapter 3. Molecular Mechanism of Secretory Vesicle Content Expulsion During Cell Secretion; Chapter 4. Fusion Pores in Growth-Hormone-Secreting Cells of the Pituitary Gland: An AFM Study; Chapter 5. Properties of Microbial Cell Surfaces Examined by Atomic Force Microscopy; Chapter 6. Scanning Probe Microscopy of Plant Cell Wall and Its Constituents; Chapter 7. Cellular Interactions of Nano Drug Delivery Systems
Chapter 8. Adapting AFM Techniques for Studies on Living CellsChapter 9. Intermolecular Forces of Leukocyte Adhesion Molecules; Chapter 10. Mechanisms of Avidity Modulation in Leukocyte Adhesion Studied by AFM; Chapter 11. Resolving the Thickness and Micromechanical Properties of Lipid Bilayers and Vesicles Using AFM; Chapter 12. Imaging Soft Surfaces by SFM; Chapter 13. High-Speed Atomic Force Microscopy of Biomolecules in Motion; Chapter 14. Atomic Force Microscopy in Cytogenetics Chapter 15. Atomic Force Microscopy in the Study of Macromolecular Interactions in Hemostasis and Thrombosis: Utility for Investigation of the Antiphospholipid SyndromeIndex |
| Record Nr. | UNINA-9910585798703321 |
| Hoboken, N.J., : Wiley-Liss, c2006 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Force microscopy [[electronic resource] ] : applications in biology and medicine / / edited by Bhanu P. Jena, J.K. Heinrich Hörber
| Force microscopy [[electronic resource] ] : applications in biology and medicine / / edited by Bhanu P. Jena, J.K. Heinrich Hörber |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley-Liss, c2006 |
| Descrizione fisica | 1 online resource (312 p.) |
| Disciplina |
502.82
610.28 |
| Altri autori (Persone) |
JenaBhanu P
HörberJ. K. Heinrich |
| Soggetto topico |
Medical electronics
Scanning force microscopy Scanning probe microscopy Nanotechnology |
| ISBN |
1-280-50788-8
9786610507887 0-470-00770-2 0-470-00769-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
FORCE MICROSCOPY; CONTENTS; Preface; Contributors; Chapter 1. Porosome: The Universal Secretory Machinery in Cells; Chapter 2. Molecular Mechanism of SNARE-Induced Membrane Fusion; Chapter 3. Molecular Mechanism of Secretory Vesicle Content Expulsion During Cell Secretion; Chapter 4. Fusion Pores in Growth-Hormone-Secreting Cells of the Pituitary Gland: An AFM Study; Chapter 5. Properties of Microbial Cell Surfaces Examined by Atomic Force Microscopy; Chapter 6. Scanning Probe Microscopy of Plant Cell Wall and Its Constituents; Chapter 7. Cellular Interactions of Nano Drug Delivery Systems
Chapter 8. Adapting AFM Techniques for Studies on Living CellsChapter 9. Intermolecular Forces of Leukocyte Adhesion Molecules; Chapter 10. Mechanisms of Avidity Modulation in Leukocyte Adhesion Studied by AFM; Chapter 11. Resolving the Thickness and Micromechanical Properties of Lipid Bilayers and Vesicles Using AFM; Chapter 12. Imaging Soft Surfaces by SFM; Chapter 13. High-Speed Atomic Force Microscopy of Biomolecules in Motion; Chapter 14. Atomic Force Microscopy in Cytogenetics Chapter 15. Atomic Force Microscopy in the Study of Macromolecular Interactions in Hemostasis and Thrombosis: Utility for Investigation of the Antiphospholipid SyndromeIndex |
| Record Nr. | UNINA-9910830250803321 |
| Hoboken, N.J., : Wiley-Liss, c2006 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Force microscopy [[electronic resource] ] : applications in biology and medicine / / edited by Bhanu P. Jena, J.K. Heinrich Hörber
| Force microscopy [[electronic resource] ] : applications in biology and medicine / / edited by Bhanu P. Jena, J.K. Heinrich Hörber |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley-Liss, c2006 |
| Descrizione fisica | 1 online resource (312 p.) |
| Disciplina |
502.82
610.28 |
| Altri autori (Persone) |
JenaBhanu P
HörberJ. K. Heinrich |
| Soggetto topico |
Medical electronics
Scanning force microscopy Scanning probe microscopy Nanotechnology |
| ISBN |
1-280-50788-8
9786610507887 0-470-00770-2 0-470-00769-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
FORCE MICROSCOPY; CONTENTS; Preface; Contributors; Chapter 1. Porosome: The Universal Secretory Machinery in Cells; Chapter 2. Molecular Mechanism of SNARE-Induced Membrane Fusion; Chapter 3. Molecular Mechanism of Secretory Vesicle Content Expulsion During Cell Secretion; Chapter 4. Fusion Pores in Growth-Hormone-Secreting Cells of the Pituitary Gland: An AFM Study; Chapter 5. Properties of Microbial Cell Surfaces Examined by Atomic Force Microscopy; Chapter 6. Scanning Probe Microscopy of Plant Cell Wall and Its Constituents; Chapter 7. Cellular Interactions of Nano Drug Delivery Systems
Chapter 8. Adapting AFM Techniques for Studies on Living CellsChapter 9. Intermolecular Forces of Leukocyte Adhesion Molecules; Chapter 10. Mechanisms of Avidity Modulation in Leukocyte Adhesion Studied by AFM; Chapter 11. Resolving the Thickness and Micromechanical Properties of Lipid Bilayers and Vesicles Using AFM; Chapter 12. Imaging Soft Surfaces by SFM; Chapter 13. High-Speed Atomic Force Microscopy of Biomolecules in Motion; Chapter 14. Atomic Force Microscopy in Cytogenetics Chapter 15. Atomic Force Microscopy in the Study of Macromolecular Interactions in Hemostasis and Thrombosis: Utility for Investigation of the Antiphospholipid SyndromeIndex |
| Record Nr. | UNINA-9910840986603321 |
| Hoboken, N.J., : Wiley-Liss, c2006 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Fundamental Properties of Semiconductor Nanowires / editors Naoki Fukata, Riccardo Rurali
| Fundamental Properties of Semiconductor Nanowires / editors Naoki Fukata, Riccardo Rurali |
| Pubbl/distr/stampa | Singapore, : Springer, 2021 |
| Descrizione fisica | VIII, 454 p. : ill. ; 24 cm |
| Disciplina |
543.65(Spettrometria di massa. Spettroscopia di massa)
543.54(Spettroscopia molecolare) 502.82(Microscopia) 620.5(Nanotecnologia) 620.1(Scienze dei materiali) 541.377(Semiconduttori) |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNICAMPANIA-VAN0243969 |
| Singapore, : Springer, 2021 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Vanvitelli | ||
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