Real-time PCR : advanced technologies and applications / / Edited by Nick A. Saunders and Martin A. Lee

• Pubblicazione: Norfolk, England : , : Caister Academic Press, , [2013]; ©2013
• Descrizione fisica: 1 online resource (289 p.)
• Disciplina: 574.873282
• Soggetto topico: Polymerase chain reaction; Molecular biology - Technique
• Persona (resp. second.): LeeMartin A.; SaundersNick (Nick A.)
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Contents; Contributors; Preface; Ch 01: Homogeneous Fluorescent Chemistries for Real-time PCR; Ch 02: Internal and Other Controls for Real-time PCR Validation; Ch 03: Analysis of mRNA Expression by Real-time PCR; Ch 04: Detection of Biothreats by Real-time PCR; Ch 05: Veterinary Applications of Real-time PCR for Detection and Diagnosis of Infectious Agents; Ch 06: Applications in Clinical Microbiology; Ch 07: The Extraction and Purification of Nucleic Acids for Analysis by PCR ; Ch 08: Oligonucleotide Primers and Probes: Use of Chemical Modifications to Increase or Decrease the Specificity of qPCRCh 09: Real-time PCR Arrays; Ch 10: The Validation of Real-time PCR Assays for Infectious Diseases; Ch 11: MIQE: Guidelines for the Design and Publication of a Reliable Real-time PCR Assay; Ch 12: Management Aspects of Real-time PCR-based Assay Development, Validation,Verification and Implementation; Index
• Sommario/riassunto: Real-time PCR technology is an established powerful research tool used in many scientific disciplines and is also utilized for mainstream testing in the regulated markets, such as food, veterinary, and human in-vitro diagnostics. This essential manual provides both the novice and experienced user with an invaluable reference to a wide-range of real-time PCR technologies and applications, and it provides an overview of the theory of this increasingly important technique. Renowned international authors present detailed technical insights into the underlying principles, methods, and practice of r
• Titolo autorizzato: Real-time PCR
• ISBN: 1-908230-87-8
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Lingua di pubblicazione: Inglese
• Record Nr.: 9910817860603321
• Lo trovi qui: Univ. Federico II