1.

Record Nr.

UNINA9910817860603321

Titolo

Real-time PCR : advanced technologies and applications / / Edited by Nick A. Saunders and Martin A. Lee

Pubbl/distr/stampa

Norfolk, England : , : Caister Academic Press, , [2013]

©2013

ISBN

1-908230-87-8

Descrizione fisica

1 online resource (289 p.)

Disciplina

574.873282

Soggetti

Polymerase chain reaction

Molecular biology - Technique

Lingua di pubblicazione

Inglese

Formato

Materiale a stampa

Livello bibliografico

Monografia

Note generali

Description based upon print version of record.

Nota di bibliografia

Includes bibliographical references and index.

Nota di contenuto

Contents; Contributors; Preface; Ch 01: Homogeneous Fluorescent Chemistries for Real-time PCR; Ch 02: Internal and Other Controls for Real-time PCR Validation; Ch 03: Analysis of mRNA Expression by Real-time PCR; Ch 04: Detection of Biothreats by Real-time PCR; Ch 05: Veterinary Applications of Real-time PCR for Detection and Diagnosis of Infectious Agents; Ch 06: Applications in Clinical Microbiology; Ch 07: The Extraction and Purification of Nucleic Acids for Analysis by PCR

Ch 08: Oligonucleotide Primers and Probes: Use of Chemical Modifications to Increase or Decrease the Specificity of qPCRCh 09: Real-time PCR Arrays; Ch 10: The Validation of Real-time PCR Assays for Infectious Diseases; Ch 11: MIQE: Guidelines for the Design and Publication of a Reliable Real-time PCR Assay; Ch 12: Management Aspects of Real-time PCR-based Assay Development, Validation,Verification and Implementation; Index

Sommario/riassunto

Real-time PCR technology is an established powerful research tool used in many scientific disciplines and is also utilized for mainstream testing in the regulated markets, such as food, veterinary, and human in-vitro diagnostics. This essential manual provides both the novice and experienced user with an invaluable reference to a wide-range of real-time PCR technologies and applications, and it provides an overview of the theory of this increasingly important technique. Renowned international authors present detailed technical insights into the



underlying principles, methods, and practice of r

2.

Record Nr.

UNINA9910254635203321

Autore

Forest Etienne

Titolo

From Tracking Code to Analysis : Generalised Courant-Snyder Theory for Any Accelerator Model / / by Etienne Forest

Pubbl/distr/stampa

Tokyo : , : Springer Japan : , : Imprint : Springer, , 2016

ISBN

4-431-55803-9

Edizione

[1st ed. 2016.]

Descrizione fisica

1 online resource (351 p.)

Disciplina

530

Soggetti

Particle acceleration

Physics

Computer programming

Particle Acceleration and Detection, Beam Physics

Numerical and Computational Physics, Simulation

Programming Techniques

Lingua di pubblicazione

Inglese

Formato

Materiale a stampa

Livello bibliografico

Monografia

Note generali

Description based upon print version of record.

Nota di bibliografia

Includes bibliographical references and index.

Nota di contenuto

Introduction -- The linear transverse normal form: one degree of freedom -- The nonlinear transverse normal form: one degree of freedom -- Classification of linear normal forms -- Nonlinear normal forms -- Spin normal form -- The nonlinear spin-orbital phase advance: the mother of all algorithms -- Deprit-Guignard perturbation theory faithful to the code -- Here is the conclusion of this book -- Phasors basis: why do I reject symplectic phasors? -- The logarithm of a map -- Stroboscopic average for the ISF vector n -- Hierarchy of Analytical Methods.

Sommario/riassunto

This book illustrates a theory well suited to tracking codes, which the author has developed over the years. Tracking codes now play a central role in the design and operation of particle accelerators. The theory is fully explained step by step with equations and actual codes that the reader can compile and run with freely available compilers. In this book, the author pursues a detailed approach based on finite “s”-maps,



since this is more natural as long as tracking codes remain at the centre of accelerator design. The hierarchical nature of software imposes a hierarchy that puts map-based perturbation theory above any other methods. The map-based approach, perhaps paradoxically, allows ultimately an implementation of the Deprit-Guignard-Schoch algorithms more faithful than anything found in the standard literature. This hierarchy of methods is not a personal choice: it follows logically from tracking codes overloaded with a truncated power series algebra package. After defining abstractly and briefly what a tracking code is, the author illustrates most of the accelerator perturbation theory using an actual code: PTC. This book may seem like a manual for PTC; however, the reader is encouraged to explore other tools as well. The presence of an actual code ensures that readers will have a tool with which they can test their understanding. Codes and examples will be available from various sites since PTC is in MAD-X (CERN) and BMAD (Cornell).