LEADER 03148nam 2200565 450 001 9910817860603321 005 20230803034150.0 010 $a1-908230-87-8 035 $a(CKB)3710000000024826 035 $a(EBL)1938102 035 $a(SSID)ssj0001085781 035 $a(PQKBManifestationID)12527572 035 $a(PQKBTitleCode)TC0001085781 035 $a(PQKBWorkID)11049359 035 $a(PQKB)11673584 035 $a(MiAaPQ)EBC1938102 035 $a(MiAaPQ)EBC5897808 035 $a(Au-PeEL)EBL5897808 035 $a(OCoLC)1048368651 035 $a(EXLCZ)993710000000024826 100 $a20191011d2013 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aReal-time PCR $eadvanced technologies and applications /$fEdited by Nick A. Saunders and Martin A. Lee 210 1$aNorfolk, England :$cCaister Academic Press,$d[2013] 210 4$dİ2013 215 $a1 online resource (289 p.) 300 $aDescription based upon print version of record. 311 $a1-908230-22-3 320 $aIncludes bibliographical references and index. 327 $aContents; Contributors; Preface; Ch 01: Homogeneous Fluorescent Chemistries for Real-time PCR; Ch 02: Internal and Other Controls for Real-time PCR Validation; Ch 03: Analysis of mRNA Expression by Real-time PCR; Ch 04: Detection of Biothreats by Real-time PCR; Ch 05: Veterinary Applications of Real-time PCR for Detection and Diagnosis of Infectious Agents; Ch 06: Applications in Clinical Microbiology; Ch 07: The Extraction and Purification of Nucleic Acids for Analysis by PCR 327 $aCh 08: Oligonucleotide Primers and Probes: Use of Chemical Modifications to Increase or Decrease the Specificity of qPCRCh 09: Real-time PCR Arrays; Ch 10: The Validation of Real-time PCR Assays for Infectious Diseases; Ch 11: MIQE: Guidelines for the Design and Publication of a Reliable Real-time PCR Assay; Ch 12: Management Aspects of Real-time PCR-based Assay Development, Validation,Verification and Implementation; Index 330 $aReal-time PCR technology is an established powerful research tool used in many scientific disciplines and is also utilized for mainstream testing in the regulated markets, such as food, veterinary, and human in-vitro diagnostics. This essential manual provides both the novice and experienced user with an invaluable reference to a wide-range of real-time PCR technologies and applications, and it provides an overview of the theory of this increasingly important technique. Renowned international authors present detailed technical insights into the underlying principles, methods, and practice of r 606 $aPolymerase chain reaction 606 $aMolecular biology$xTechnique 615 0$aPolymerase chain reaction. 615 0$aMolecular biology$xTechnique. 676 $a574.873282 702 $aLee$b Martin A. 702 $aSaunders$b Nick$g(Nick A.), 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910817860603321 996 $aReal-time PCR$9762569 997 $aUNINA