Bioanalytical chemistry / / Susan R. Mikkelsen, Eduardo Cortón

• Autore: Mikkelsen Susan R. <1960->
• Pubblicazione: Hoboken, New Jersey : , : John Wiley & Sons, Incorporated, , [2016]; ©2016
• Edizione: Second edition.
• Descrizione fisica: 1 online resource (641 p.)
• Disciplina: 612/.01585
• Soggetto topico: Analytical biochemistry; Chemistry, Analytic
• Soggetto genere / forma: Electronic books.
• Persona (resp. second.): CortónEduardo <1962->
• Note generali: Includes index.
• Nota di contenuto: Title Page; Copyright; Preface to Second Edition; Preface to First Edition; Acknowledgments; 1: Quantitative Instrumental Measurements; 1.1 Introduction; 1.2 Optical Measurements; 1.3 Electrochemical Measurements; 1.4 Radiochemical Measurements; 1.5 Surface Plasmon Resonance; 1.6 Calorimetry; 1.7 Automation: Microplates, Multiwell Liquid Dispensers and Microplate Readers; 1.8 Calibration of Instrumental Measurements; 1.9 Quantitative and Semi-Quantitative Measurements; Suggested Reading; Problems; 2: Spectroscopic Methods for the Quantitation of Classes of Biomolecules; 2.1 Introduction ; 2.2 Total Protein2.3 Total DNA; 2.4 Total RNA; 2.5 Total Carbohydrate; 2.6 Free Fatty Acids; References; Problems; 3: Enzymes; 3.1 Introduction; 3.2 Enzyme Nomenclature; 3.3 Enzyme Commission Numbers; 3.4 Enzymes in Bioanalytical Chemistry; 3.5 Enzyme Kinetics; 3.6 Enzyme Activators; 3.7 Enzyme Inhibitors; 3.8 Enzyme Units and Concentrations; Suggested Reading; References; Problems; 4: Quantitation of Enzymes and Their Substrates; 4.1 Introduction; 4.2 Substrate Depletion or Product Accumulation; 4.3 Direct and Coupled Measurements; 4.4 Classification of Methods; 4.5 Instrumental Methods ; 4.6 High-Throughput Assays for Enzymes and Inhibitors4.7. Assays for Enzymatic Reporter Gene Products27-30; 4.8 Practical Considerations for Enzymatic Assays; Suggested Reading; References; Problems; 5: Immobilized Enzymes; 5.1 Introduction; 5.2 Immobilization Methods; 5.3 Properties of Immobilized Enzymes; 5.4 Immobilized Enzyme Reactors; 5.5 Theoretical Treatment of Packed-Bed Enzyme Reactors34-37; Suggested Reading; References; Problems; 6: Antibodies; 6.1 Introduction; 6.2 Structural and Functional Properties of Antibodies; 6.3 Polyclonal and Monoclonal Antibodies ; 6.4 Antibody-Antigen Interactions6.5 Analytical Applications of Secondary Antibody-Antigen Interactions; Suggested Reading; References; Problems; 7: Quantitative Immunoassays with Labels; 7.1 Introduction; 7.2 Labeling Reactions; 7.3 Heterogeneous Immunoassays; 7.4 Homogeneous Immunoassays; 7.5 Evaluation of New Immunoassay Methods33,34; Suggested Reading; References; Problems; 8: Biosensors; 8.1 Introduction; 8.2 Biosensor Diversity and Classification; 8.3 Recognition Agents; 8.4 Response of Enzyme-Based Biosensors5; 8.5 Examples of Biosensor Configurations ; 8.6 Evaluation of Biosensor Perfomance8.7 In VIVO Applications of Biosensors; Suggested Reading; References; Problems; 9: Directed Evolution for the Design of Macromolecular Reagents; 9.1 Introduction; 9.2 Rational Design and Directed Evolution; 9.3 Generation of Genetic Diversity; 9.4 Linking Genotype and Phenotype; 9.5 Identification and Selection of Successful Variants; 9.6 Examples of Directed Evolution Experiments; Suggested Reading; References; Problems; 10: Image-Based Bioanalysis; 10.1 Introduction; 10.2 Magnification and Resolution; 10.3 Optical Microscopy; 10.4 Electron Microscopy ; 10.5 Scanning Tunneling Microscopy
• Titolo autorizzato: Bioanalytical chemistry
• ISBN: 1-119-05770-1; 1-119-05763-9
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Lingua di pubblicazione: Inglese
• Record Nr.: 9910465872703321
• Lo trovi qui: Univ. Federico II