LEADER 04810nam 2200685 450 001 9910465872703321 005 20200520144314.0 010 $a1-119-05770-1 010 $a1-119-05763-9 035 $a(CKB)3710000000603861 035 $a(EBL)4413726 035 $a(SSID)ssj0001614080 035 $a(PQKBManifestationID)16340548 035 $a(PQKBTitleCode)TC0001614080 035 $a(PQKBWorkID)14915030 035 $a(PQKB)11112204 035 $a(PQKBManifestationID)16346246 035 $a(PQKB)21053001 035 $a(MiAaPQ)EBC4413726 035 $a(DLC) 2015043820 035 $a(JP-MeL)3000065185 035 $a(Au-PeEL)EBL4413726 035 $a(CaPaEBR)ebr11161293 035 $a(CaONFJC)MIL900903 035 $a(OCoLC)940934954 035 $a(EXLCZ)993710000000603861 100 $a20151105h20162016 uy| 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aBioanalytical chemistry /$fSusan R. Mikkelsen, Eduardo Corto?n 205 $aSecond edition. 210 1$aHoboken, New Jersey :$cJohn Wiley & Sons, Incorporated,$d[2016] 210 4$dİ2016 215 $a1 online resource (641 p.) 300 $aIncludes index. 311 $a1-118-30254-0 327 $aTitle Page; Copyright; Preface to Second Edition; Preface to First Edition; Acknowledgments; 1: Quantitative Instrumental Measurements; 1.1 Introduction; 1.2 Optical Measurements; 1.3 Electrochemical Measurements; 1.4 Radiochemical Measurements; 1.5 Surface Plasmon Resonance; 1.6 Calorimetry; 1.7 Automation: Microplates, Multiwell Liquid Dispensers and Microplate Readers; 1.8 Calibration of Instrumental Measurements; 1.9 Quantitative and Semi-Quantitative Measurements; Suggested Reading; Problems; 2: Spectroscopic Methods for the Quantitation of Classes of Biomolecules; 2.1 Introduction 327 $a2.2 Total Protein2.3 Total DNA; 2.4 Total RNA; 2.5 Total Carbohydrate; 2.6 Free Fatty Acids; References; Problems; 3: Enzymes; 3.1 Introduction; 3.2 Enzyme Nomenclature; 3.3 Enzyme Commission Numbers; 3.4 Enzymes in Bioanalytical Chemistry; 3.5 Enzyme Kinetics; 3.6 Enzyme Activators; 3.7 Enzyme Inhibitors; 3.8 Enzyme Units and Concentrations; Suggested Reading; References; Problems; 4: Quantitation of Enzymes and Their Substrates; 4.1 Introduction; 4.2 Substrate Depletion or Product Accumulation; 4.3 Direct and Coupled Measurements; 4.4 Classification of Methods; 4.5 Instrumental Methods 327 $a4.6 High-Throughput Assays for Enzymes and Inhibitors4.7. Assays for Enzymatic Reporter Gene Products27-30; 4.8 Practical Considerations for Enzymatic Assays; Suggested Reading; References; Problems; 5: Immobilized Enzymes; 5.1 Introduction; 5.2 Immobilization Methods; 5.3 Properties of Immobilized Enzymes; 5.4 Immobilized Enzyme Reactors; 5.5 Theoretical Treatment of Packed-Bed Enzyme Reactors34-37; Suggested Reading; References; Problems; 6: Antibodies; 6.1 Introduction; 6.2 Structural and Functional Properties of Antibodies; 6.3 Polyclonal and Monoclonal Antibodies 327 $a6.4 Antibody-Antigen Interactions6.5 Analytical Applications of Secondary Antibody-Antigen Interactions; Suggested Reading; References; Problems; 7: Quantitative Immunoassays with Labels; 7.1 Introduction; 7.2 Labeling Reactions; 7.3 Heterogeneous Immunoassays; 7.4 Homogeneous Immunoassays; 7.5 Evaluation of New Immunoassay Methods33,34; Suggested Reading; References; Problems; 8: Biosensors; 8.1 Introduction; 8.2 Biosensor Diversity and Classification; 8.3 Recognition Agents; 8.4 Response of Enzyme-Based Biosensors5; 8.5 Examples of Biosensor Configurations 327 $a8.6 Evaluation of Biosensor Perfomance8.7 In VIVO Applications of Biosensors; Suggested Reading; References; Problems; 9: Directed Evolution for the Design of Macromolecular Reagents; 9.1 Introduction; 9.2 Rational Design and Directed Evolution; 9.3 Generation of Genetic Diversity; 9.4 Linking Genotype and Phenotype; 9.5 Identification and Selection of Successful Variants; 9.6 Examples of Directed Evolution Experiments; Suggested Reading; References; Problems; 10: Image-Based Bioanalysis; 10.1 Introduction; 10.2 Magnification and Resolution; 10.3 Optical Microscopy; 10.4 Electron Microscopy 327 $a10.5 Scanning Tunneling Microscopy 606 $aAnalytical biochemistry 606 $aChemistry, Analytic 608 $aElectronic books. 615 0$aAnalytical biochemistry. 615 0$aChemistry, Analytic. 676 $a612/.01585 700 $aMikkelsen$b Susan R.$f1960-$0937604 702 $aCorto?n$b Eduardo$f1962- 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910465872703321 996 $aBioanalytical chemistry$92111891 997 $aUNINA