04810nam 2200685 450 991046587270332120200520144314.01-119-05770-11-119-05763-9(CKB)3710000000603861(EBL)4413726(SSID)ssj0001614080(PQKBManifestationID)16340548(PQKBTitleCode)TC0001614080(PQKBWorkID)14915030(PQKB)11112204(PQKBManifestationID)16346246(PQKB)21053001(MiAaPQ)EBC4413726(DLC) 2015043820(JP-MeL)3000065185(Au-PeEL)EBL4413726(CaPaEBR)ebr11161293(CaONFJC)MIL900903(OCoLC)940934954(EXLCZ)99371000000060386120151105h20162016 uy| 0engur|n|---|||||txtccrBioanalytical chemistry /Susan R. Mikkelsen, Eduardo CortónSecond edition.Hoboken, New Jersey :John Wiley & Sons, Incorporated,[2016]©20161 online resource (641 p.)Includes index.1-118-30254-0 Title Page; Copyright; Preface to Second Edition; Preface to First Edition; Acknowledgments; 1: Quantitative Instrumental Measurements; 1.1 Introduction; 1.2 Optical Measurements; 1.3 Electrochemical Measurements; 1.4 Radiochemical Measurements; 1.5 Surface Plasmon Resonance; 1.6 Calorimetry; 1.7 Automation: Microplates, Multiwell Liquid Dispensers and Microplate Readers; 1.8 Calibration of Instrumental Measurements; 1.9 Quantitative and Semi-Quantitative Measurements; Suggested Reading; Problems; 2: Spectroscopic Methods for the Quantitation of Classes of Biomolecules; 2.1 Introduction2.2 Total Protein2.3 Total DNA; 2.4 Total RNA; 2.5 Total Carbohydrate; 2.6 Free Fatty Acids; References; Problems; 3: Enzymes; 3.1 Introduction; 3.2 Enzyme Nomenclature; 3.3 Enzyme Commission Numbers; 3.4 Enzymes in Bioanalytical Chemistry; 3.5 Enzyme Kinetics; 3.6 Enzyme Activators; 3.7 Enzyme Inhibitors; 3.8 Enzyme Units and Concentrations; Suggested Reading; References; Problems; 4: Quantitation of Enzymes and Their Substrates; 4.1 Introduction; 4.2 Substrate Depletion or Product Accumulation; 4.3 Direct and Coupled Measurements; 4.4 Classification of Methods; 4.5 Instrumental Methods4.6 High-Throughput Assays for Enzymes and Inhibitors4.7. Assays for Enzymatic Reporter Gene Products27-30; 4.8 Practical Considerations for Enzymatic Assays; Suggested Reading; References; Problems; 5: Immobilized Enzymes; 5.1 Introduction; 5.2 Immobilization Methods; 5.3 Properties of Immobilized Enzymes; 5.4 Immobilized Enzyme Reactors; 5.5 Theoretical Treatment of Packed-Bed Enzyme Reactors34-37; Suggested Reading; References; Problems; 6: Antibodies; 6.1 Introduction; 6.2 Structural and Functional Properties of Antibodies; 6.3 Polyclonal and Monoclonal Antibodies6.4 Antibody-Antigen Interactions6.5 Analytical Applications of Secondary Antibody-Antigen Interactions; Suggested Reading; References; Problems; 7: Quantitative Immunoassays with Labels; 7.1 Introduction; 7.2 Labeling Reactions; 7.3 Heterogeneous Immunoassays; 7.4 Homogeneous Immunoassays; 7.5 Evaluation of New Immunoassay Methods33,34; Suggested Reading; References; Problems; 8: Biosensors; 8.1 Introduction; 8.2 Biosensor Diversity and Classification; 8.3 Recognition Agents; 8.4 Response of Enzyme-Based Biosensors5; 8.5 Examples of Biosensor Configurations8.6 Evaluation of Biosensor Perfomance8.7 In VIVO Applications of Biosensors; Suggested Reading; References; Problems; 9: Directed Evolution for the Design of Macromolecular Reagents; 9.1 Introduction; 9.2 Rational Design and Directed Evolution; 9.3 Generation of Genetic Diversity; 9.4 Linking Genotype and Phenotype; 9.5 Identification and Selection of Successful Variants; 9.6 Examples of Directed Evolution Experiments; Suggested Reading; References; Problems; 10: Image-Based Bioanalysis; 10.1 Introduction; 10.2 Magnification and Resolution; 10.3 Optical Microscopy; 10.4 Electron Microscopy10.5 Scanning Tunneling MicroscopyAnalytical biochemistryChemistry, AnalyticElectronic books.Analytical biochemistry.Chemistry, Analytic.612/.01585Mikkelsen Susan R.1960-937604Cortón Eduardo1962-MiAaPQMiAaPQMiAaPQBOOK9910465872703321Bioanalytical chemistry2111891UNINA