Membrane protein purification and crystallization [[electronic resource] ] : a practical guide / / edited by Carola Hunte and Gebhard von Jagow and Hermann Schagger

• Pubblicazione: Amsterdam ; ; Boston, : Academic Press, c2003
• Edizione: 2nd ed.
• Descrizione fisica: 1 online resource (339 p.)
• Disciplina: 572/.696
• Soggetto topico: Membrane proteins - Purification
• Soggetto genere / forma: Electronic books.
• Altri autori: JagowGebhard von SchäggerHermann
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Front Cover; Membrane Protein Purification and Crystallization: A Practical Guide; Copyright Page; Contents; Contributors; Preface; PART I: STRATEGIES AND TECHNIQUES; Chapter 1. Purification Strategies for Membrane Proteins; I. Introduction; II. General Guide for Retaining Catalytic Activity; III. Choice and Sequence of Purification Techniques; IV. Choice of Protein Source, Disruption of Cells, and Preparation of Organelles and Membranes; V. Protein Assay; VI. Solubilization and Stabilization of Membrane Proteins; References ; Chapter 2. Techniques and Basic Operations in Membrane Protein Purification I. Introduction; II. Solubilization-Precipitation; III. Concentration of Samples, Exchange of Buffer, and Exchange of Detergent; IV. Chromatographic Techniques; References; Chapter 3. Production and Purification of Recombinant Membrane Proteins; I. Introduction; II. Prokaryotic Expression Systems for Overproduction of Membrane Proteins for Structural Studies; III. Eukaryotic Expression Systems for Overproduction of Membrane Proteins; IV. Use of Fusion Proteins and Affinity Tags for Purification of Membrane Proteins ; Acknowledgments References; Chapter 4. SDS Electrophoresis Techniques; I. Introduction; II. Choice of Optimal SDS-Page System and Gel Type; III. Tricine-SDS-PAGE; IV. Recovery of Proteins from SDS Gels; V. Processing of Proteins Recovered from Gels for Immunization and Protein Sequencing; VI. Electroblotting; References; Chapter 5. Blue Native Electrophoresis; I. Introduction; II. Techniques; III. Applications; References; Chapter 6. Preparative Isoelectric Focusing; I. Introduction; II. Techniques; III. Application: Purification of Photosystem I; IV. Conclusions; References ; Chapter 7. Membrane Protein CrystallizationI. Introduction; II. Bottlenecks in Membrane Protein Crystallization; References; PART II: DISCUSSION OF SELECTED ISOLATION PROTOCOLS; Chapter 8. Lipid-Dependent Inactivation and Reactivation of Bovine Complex III; I. Introduction; II. Lipid-Dependent Inactivation and Reactivation; References; Chapter 9. Purification of an Affinity-Epitope Tagged G-Protein Coupled Receptor; I. Introduction; II. Production of Recombinant β2-Adrenergic Receptor in Pichia pastoris; III. Preparation of Pichia pastoris Membranes; IV. Solubilization of Membranes ; V. Purification of the β2-Adrenergic Receptor VI. Ligand Binding Assays; References; Chapter 10. Purification of NhaA Na+ /H+ Antiporter of Escherichia Coli for 3D or 2D Crystallization; I. Introduction; II. Growth of Escherichia coli for Production of His-Tagged NhaA in a 10 1 Fermenter; III. Isolation of Membranes from Escherichia coli TA16/pAXH Strain; IV. Two-Step Purification of His-Tagged NHaA; V. NhaA Reconstitution into Liposomes and Activity Assay; VI. Dynamic Light Scattering Experiments; Acknowledgments; References; Chapter 11. Purification of the Cytochrome bc1 Complex from Yeast ; I. Introduction
• Sommario/riassunto: This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new
• Titolo autorizzato: Membrane protein purification and crystallization
• ISBN: 1-281-02929-7; 9786611029296; 0-08-053617-4
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Lingua di pubblicazione: Inglese
• Record Nr.: 9910458689403321
• Lo trovi qui: Univ. Federico II