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[null,"1.","Record Nr.",null,"UNINA9910458689403321"]
[null,null,"Titolo",null,"Membrane protein purification and crystallization [[electronic resource] ] : a practical guide / / edited by Carola Hunte and Gebhard von Jagow and Hermann Schagger"]
[null,null,"Pubbl/distr/stampa",null,"Amsterdam ; ; Boston, : Academic Press, c2003"]
[null,null,"ISBN",null,"1-281-02929-7"]
[{"$nulls":4},"9786611029296"]
[{"$nulls":4},"0-08-053617-4"]
[null,null,"Edizione",null,"[2nd ed.]"]
[null,null,"Descrizione fisica",null,"1 online resource (339 p.)"]
[null,null,"Disciplina",null,"572/.696"]
[null,null,"Soggetti",null,"Membrane proteins - Purification"]
[{"$nulls":4},"Electronic books."]
[null,null,"Lingua di pubblicazione",null,"Inglese"]
[null,null,"Formato",null,"Materiale a stampa"]
[null,null,null,"Livello bibliografico","Monografia"]
[null,null,null,"Note generali","Description based upon print version of record."]
[null,null,null,"Nota di bibliografia","Includes bibliographical references and index."]
[null,null,"Nota di contenuto",null,"Front Cover; Membrane Protein Purification and Crystallization: A Practical Guide; Copyright Page; Contents; Contributors; Preface; PART I: STRATEGIES AND TECHNIQUES; Chapter 1. Purification Strategies for Membrane Proteins; I. Introduction; II. General Guide for Retaining Catalytic Activity; III. Choice and Sequence of Purification Techniques; IV. Choice of Protein Source, Disruption of Cells, and Preparation of Organelles and Membranes; V. Protein Assay; VI. Solubilization and Stabilization of Membrane Proteins; References"]
[{"$nulls":4},"Chapter 2. Techniques and Basic Operations in Membrane Protein Purification I. Introduction; II. Solubilization-Precipitation; III. Concentration of Samples, Exchange of Buffer, and Exchange of Detergent; IV. Chromatographic Techniques; References; Chapter 3. Production and Purification of Recombinant Membrane Proteins; I. Introduction; II. Prokaryotic Expression Systems for Overproduction of Membrane Proteins for Structural Studies; III. Eukaryotic Expression Systems for Overproduction of Membrane Proteins; IV. Use of Fusion Proteins and Affinity Tags for Purification of Membrane Proteins"]
[{"$nulls":4},"Acknowledgments References; Chapter 4. SDS Electrophoresis Techniques; I. Introduction; II. Choice of Optimal SDS-Page System and Gel Type; III. Tricine-SDS-PAGE; IV. Recovery of Proteins from SDS Gels; V. Processing of Proteins Recovered from Gels for Immunization and Protein Sequencing; VI. Electroblotting; References; Chapter 5. Blue "]
[{"$nulls":4},"Native Electrophoresis; I. Introduction; II. Techniques; III. Applications; References; Chapter 6. Preparative Isoelectric Focusing; I. Introduction; II. Techniques; III. Application: Purification of Photosystem I; IV. Conclusions; References"]
[{"$nulls":4},"Chapter 7. Membrane Protein CrystallizationI. Introduction; II. Bottlenecks in Membrane Protein Crystallization; References; PART II: DISCUSSION OF SELECTED ISOLATION PROTOCOLS; Chapter 8. Lipid-Dependent Inactivation and Reactivation of Bovine Complex III; I. Introduction; II. Lipid-Dependent Inactivation and Reactivation; References; Chapter 9. Purification of an Affinity-Epitope Tagged G-Protein Coupled Receptor; I. Introduction; II. Production of Recombinant β2-Adrenergic Receptor in Pichia pastoris; III. Preparation of Pichia pastoris Membranes; IV. Solubilization of Membranes"]
[{"$nulls":4},"V. Purification of the β2-Adrenergic Receptor VI. Ligand Binding Assays; References; Chapter 10. Purification of NhaA Na+ /H+ Antiporter of Escherichia Coli for 3D or 2D Crystallization; I. Introduction; II. Growth of Escherichia coli for Production of His-Tagged NhaA in a 10 1 Fermenter; III. Isolation of Membranes from Escherichia coli TA16/pAXH Strain; IV. Two-Step Purification of His-Tagged NHaA; V. NhaA Reconstitution into Liposomes and Activity Assay; VI. Dynamic Light Scattering Experiments; Acknowledgments; References; Chapter 11. Purification of the Cytochrome bc1 Complex from Yeast"]
[{"$nulls":4},"I. Introduction"]
[null,null,null,"Sommario/riassunto","This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new"]
[null,"2.","Record Nr.",null,"UNINA9910461094003321"]
[null,null,"Autore",null,"Lambert Leonard Carson"]
[null,null,"Titolo",null,"Up from these hills [[electronic resource] ] : memories of a Cherokee boyhood / / Leonard Carson Lambert Jr. ; as told to Michael Lambert"]
[null,null,"Pubbl/distr/stampa",null,"Lincoln [Neb.], : University of Nebraska Press, c2011"]
[null,null,"ISBN",null,"0-8032-6793-2"]
[{"$nulls":4},"1-280-54710-3"]
[{"$nulls":4},"9786613596208"]
[{"$nulls":4},"0-8032-3770-7"]
[null,null,"Descrizione fisica",null,"1 online resource (236 p.)"]
[null,null,"Collana",null,"Indians of the Southeast"]
[null,null,"Disciplina",null,"975.004/97557"]
[{"$nulls":4},"B"]
[null,null,"Soggetti",null,"Cherokee Indians"]
[{"$nulls":4},"Electronic books."]
[null,null,"Lingua di pubblicazione",null,"Inglese"]
[null,null,"Formato",null,"Materiale a stampa"]
[null,null,null,"Livello bibliografico","Monografia"]
[null,null,null,"Note generali","Description based upon print version of record."]
[null,null,null,"Nota di bibliografia","Includes bibliographical references (p. [193]-197)."]
[null,null,"Nota di contenuto",null,"Cover; Title Page; Copyright Page; Contents; Series Preface; Forethought's; Smith-Lambert Family Genealogy; Roots; The Cove; Tennessee; Mentor School; Mars Hill; Going Home; Notes"]
[null,null,null,"Sommario/riassunto","Born into a storied but impoverished family on the reservation of the Eastern Band of Cherokee Indians, Leonard Carson Lambert Jr.'s candid memoir is a remarkable story and an equally remarkable flouting of the stereotypes that so many tales of American Indian life have engendered. Up from These Hills provides a grounded, yet poignant, description of what it was like to grow up during the 1930's and 1940's in the mountains of western North Carolina and on a sharecropper's farm in eastern Tennessee. Lambert straightforwardly describes his independent, hardworking, and stubborn parents;"]
[null,"3.","Record Nr.",null,"UNINA9910783482503321"]
[null,null,"Titolo",null,"The book of Leviticus [[electronic resource] ] : composition and reception / / edited by Rolf Rendtorff and Robert A. Kugler ; with the assistance of Sarah Smith Bartel"]
[null,null,"Pubbl/distr/stampa",null,"Leiden ; ; Boston, : Brill, 2003"]
[null,null,"ISBN",null,"1-280-46690-1"]
[{"$nulls":4},"9786610466900"]
[{"$nulls":4},"1-4175-1054-4"]
[{"$nulls":4},"90-474-0164-6"]
[null,null,"Descrizione fisica",null,"1 online resource (494 p.)"]
[null,null,"Collana",null,"Supplements to Vetus Testamentum, , 0083-5889 ; ; v. 93. Formation and interpretation of Old Testament literature ; ; 3"]
[null,null,"Disciplina",null,"222/.1306"]
[null,null,"Lingua di pubblicazione",null,"Inglese"]
[null,null,"Formato",null,"Materiale a stampa"]
[null,null,null,"Livello bibliografico","Monografia"]
[null,null,null,"Note generali","Description based upon print version of record."]
[null,null,null,"Nota di bibliografia","Includes bibliographical references and indexes."]
[null,null,"Nota di contenuto",null,"Preliminary material / Editors THE BOOK OF LEVITICUS -- INTRODUCTION / ROLF RENDTORFF -- LEVITICUS: ITS LITERARY HISTORY AND LOCATION IN BIBLICAL LITERATURE / BARUCH A. LEVINE -- HR IN LEVITICUS AND ELSEWHERE IN THE TORAH / JACOB MILGROM -- LEVITICUS: AFTER EXODUS AND BEFORE NUMBERS / GRAEME AULD -- THE STRUCTURE OF THE BOOK OF LEVITICUS IN THE NARRATIVE OUTLINE OF THE PRIESTLY SINAI STORY (EXOD 19:1-NUM 10:10*) / ANDREAS RUWE -- THE RHETORIC OF RITUAL INSTRUCTION IN LEVITICUS 1-7 / JAMES W. WATTS -- THE THEOLOGY OF THE SACRIFICE ACCORDING TO LEVITICUS 1-7* / ALFRED MARX -- THE GO-AWAY GOAT / MARY DOUGLAS -- TOWARDS AN INTEGRATED READING OF THE DIETARY LAWS OF LEVITICUS / WALTER J. HOUSTON -- WHAT CONNECTS THE INCEST PROHIBITIONS WITH THE OTHER PROHIBITIONS LISTED IN LEVITICUS 18 AND 20? / ADRIAN SCHENKER -- LE SACERDOCE / RENÉ PÉTER-CONTESSE -- THE PRIESTS IN LEVITICUS-IS THE MEDIUM THE MESSAGE? / LESTER L. GRABBE -- DEATH AND SEXUALITY AMONG PRIESTS (LEVITICUS 21) / CALUM CARMICHAEL -- THE OLD GREEK TRANSLATION OF LEVITICUS / SARIANNA METSO and EUGENE ULRICH -- RECEPTION OF THE HEBREW TEXT OF LEVITICUS IN THE TARGUMS / MARTIN McNAMARA -- THE RECEPTION OF LEVITICUS: PESHITTA VERSION / D. J. LANE -- THE BOOK OF LEVITICUS IN THE DEAD SEA SCROLLS / PETER W. FLINT -- RETHINKING THE NOTION OF "]
[{"$nulls":4},"\\'SCRIPTURE\\' IN THE DEAD SEA SCROLLS: LEVITICUS AS A TEST CASE / ROBERT A. KUGLER -- JESUS, LEVITICAL PURITY, AND THE DEVELOPMENT OF PRIMITIVE CHRISTIANITY / BRUCE CHILTON -- THE RABBINIC RECEPTION OF LEVITICUS / HANNAH HARRINGTON -- ﬣﬢﬣי ﬡני: GOD'S SELF-INTRODUCTORY FORMUlA IN LEVITICUS IN MID RASH SIFRA / GERHARD BODENDORFER -- DOUBLE TIME ... DOUBLE TROUBLE? GENDER, SIN, AND LEVITICUS 12 / LINDA S. SCHEARING -- \\'COMING BEFORE THE LORD\\': THE EXCLUSION OF WOMEN FROM THE PUBLIC DOMAIN OF THE ISRAELITE PRIESTLY CULT / JUDITH ROMNEY WEGNER -- INDEX OF SELECTED SCRIPTURAL CITATIONS AND ANCIENT SOURCES / Editors THE BOOK OF LEVITICUS -- INDEX OF MODERN AUTHORS / Editors THE BOOK OF LEVITICUS."]
[null,null,null,"Sommario/riassunto","This volume examines the formation, final form, themes, and interpretation of the Book of Leviticus. Contributors include well-known experts on Leviticus: Baruch Levine, Jacob Milgrom, Graeme Auld, Andreas Ruwe, and James Watts address Leviticus in its compositional and literary context; Alfred Marx, Mary Douglas, Walter Houston, and Adrian Schenker treat issues of cult and sacrifice; and Rene Peter-Contesse, Lester Grabbe, and Calum Carmichael discuss Leviticus on the priesthood. A groundbreaking section on Leviticus in translation and interpretation includes essays by Sarianna Metso and Eugene Ulrich, Martin McNamara, David Lane, Peter Flint, Robert Kugler, Bruce Chilton, Hannah Harrington, Gerhard Bodendorfer, Linda Schearing, and Judith Romney Wegner. These essays will serve students of Leviticus well for long time to come."]