LEADER 05418nam 22006614a 450 001 9910458689403321 005 20200520144314.0 010 $a1-281-02929-7 010 $a9786611029296 010 $a0-08-053617-4 035 $a(CKB)1000000000364272 035 $a(EBL)311477 035 $a(OCoLC)476098812 035 $a(SSID)ssj0000200994 035 $a(PQKBManifestationID)11190118 035 $a(PQKBTitleCode)TC0000200994 035 $a(PQKBWorkID)10232098 035 $a(PQKB)10704446 035 $a(MiAaPQ)EBC311477 035 $a(PPN)182572250 035 $a(Au-PeEL)EBL311477 035 $a(CaPaEBR)ebr10190086 035 $a(CaONFJC)MIL102929 035 $a(EXLCZ)991000000000364272 100 $a20020104d2003 uy 0 101 0 $aeng 135 $aurcn||||||||| 181 $ctxt 182 $cc 183 $acr 200 00$aMembrane protein purification and crystallization$b[electronic resource] $ea practical guide /$fedited by Carola Hunte and Gebhard von Jagow and Hermann Schagger 205 $a2nd ed. 210 $aAmsterdam ;$aBoston $cAcademic Press$dc2003 215 $a1 online resource (339 p.) 300 $aDescription based upon print version of record. 311 $a1-4933-0395-3 311 $a0-12-361776-6 320 $aIncludes bibliographical references and index. 327 $aFront Cover; Membrane Protein Purification and Crystallization: A Practical Guide; Copyright Page; Contents; Contributors; Preface; PART I: STRATEGIES AND TECHNIQUES; Chapter 1. Purification Strategies for Membrane Proteins; I. Introduction; II. General Guide for Retaining Catalytic Activity; III. Choice and Sequence of Purification Techniques; IV. Choice of Protein Source, Disruption of Cells, and Preparation of Organelles and Membranes; V. Protein Assay; VI. Solubilization and Stabilization of Membrane Proteins; References 327 $aChapter 2. Techniques and Basic Operations in Membrane Protein Purification I. Introduction; II. Solubilization-Precipitation; III. Concentration of Samples, Exchange of Buffer, and Exchange of Detergent; IV. Chromatographic Techniques; References; Chapter 3. Production and Purification of Recombinant Membrane Proteins; I. Introduction; II. Prokaryotic Expression Systems for Overproduction of Membrane Proteins for Structural Studies; III. Eukaryotic Expression Systems for Overproduction of Membrane Proteins; IV. Use of Fusion Proteins and Affinity Tags for Purification of Membrane Proteins 327 $aAcknowledgments References; Chapter 4. SDS Electrophoresis Techniques; I. Introduction; II. Choice of Optimal SDS-Page System and Gel Type; III. Tricine-SDS-PAGE; IV. Recovery of Proteins from SDS Gels; V. Processing of Proteins Recovered from Gels for Immunization and Protein Sequencing; VI. Electroblotting; References; Chapter 5. Blue Native Electrophoresis; I. Introduction; II. Techniques; III. Applications; References; Chapter 6. Preparative Isoelectric Focusing; I. Introduction; II. Techniques; III. Application: Purification of Photosystem I; IV. Conclusions; References 327 $aChapter 7. Membrane Protein CrystallizationI. Introduction; II. Bottlenecks in Membrane Protein Crystallization; References; PART II: DISCUSSION OF SELECTED ISOLATION PROTOCOLS; Chapter 8. Lipid-Dependent Inactivation and Reactivation of Bovine Complex III; I. Introduction; II. Lipid-Dependent Inactivation and Reactivation; References; Chapter 9. Purification of an Affinity-Epitope Tagged G-Protein Coupled Receptor; I. Introduction; II. Production of Recombinant ?2-Adrenergic Receptor in Pichia pastoris; III. Preparation of Pichia pastoris Membranes; IV. Solubilization of Membranes 327 $aV. Purification of the ?2-Adrenergic Receptor VI. Ligand Binding Assays; References; Chapter 10. Purification of NhaA Na+ /H+ Antiporter of Escherichia Coli for 3D or 2D Crystallization; I. Introduction; II. Growth of Escherichia coli for Production of His-Tagged NhaA in a 10 1 Fermenter; III. Isolation of Membranes from Escherichia coli TA16/pAXH Strain; IV. Two-Step Purification of His-Tagged NHaA; V. NhaA Reconstitution into Liposomes and Activity Assay; VI. Dynamic Light Scattering Experiments; Acknowledgments; References; Chapter 11. Purification of the Cytochrome bc1 Complex from Yeast 327 $aI. Introduction 330 $aThis second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new 606 $aMembrane proteins$xPurification 608 $aElectronic books. 615 0$aMembrane proteins$xPurification. 676 $a572/.696 701 $aJagow$b Gebhard von$0868727 701 $aScha?gger$b Hermann$0868728 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910458689403321 996 $aMembrane protein purification and crystallization$91939272 997 $aUNINA