Info
- Utilizzare la checkbox di selezione a fianco di ciascun documento per attivare le funzionalità di stampa, invio email, download nei formati disponibili del (i) record.
Protein engineering : tools and applications / edited by Huimin Zhao
| Protein engineering : tools and applications / edited by Huimin Zhao |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, c2021 |
| Descrizione fisica | xii, 416 p. : ill. ; 24 cm |
| Disciplina | 660.63 |
| Collana | Advanced Biotechnology |
| Soggetto non controllato | Ingegneria biochimica |
| ISBN |
9783527815111 (ePub ebook) : 135.00
9783527344703 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910566195603321 |
| Weinheim, : Wiley-VCH, c2021 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Protein engineering : tools and applications / / edited by Huimin Zhao
| Protein engineering : tools and applications / / edited by Huimin Zhao |
| Pubbl/distr/stampa | Weinheim, Germany : , : Wiley-VCH, , [2021] |
| Descrizione fisica | 1 online resource (431 pages) |
| Disciplina | 660.63 |
| Collana | Advanced biotechnology |
| Soggetto topico | Protein engineering |
| ISBN |
3-527-81509-0
3-527-81512-0 3-527-81511-2 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910830303903321 |
| Weinheim, Germany : , : Wiley-VCH, , [2021] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Protein engineering : technology and application / / edited by Tomohisa Ogawa
| Protein engineering : technology and application / / edited by Tomohisa Ogawa |
| Pubbl/distr/stampa | Rijeka, Croatia : , : IntechOpen, , [2013] |
| Descrizione fisica | 1 online resource (196 pages) : illustrations |
| Disciplina | 660.63 |
| Soggetto topico | Protein engineering |
| ISBN | 953-51-5376-5 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Altri titoli varianti | Protein Engineering |
| Record Nr. | UNINA-9910317738803321 |
| Rijeka, Croatia : , : IntechOpen, , [2013] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Protein engineering / / edited by Pravin Kaumaya
| Protein engineering / / edited by Pravin Kaumaya |
| Pubbl/distr/stampa | Rijeka, Croatia : , : IntechOpen, , [2012] |
| Descrizione fisica | 1 online resource (356 pages) : illustrations |
| Disciplina | 660.63 |
| Soggetto topico | Protein engineering |
| ISBN | 953-51-5209-2 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910317644803321 |
| Rijeka, Croatia : , : IntechOpen, , [2012] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Protein engineering : principles and practice / edited by Jeffrey L. Cleland, Charles S. Craik
| Protein engineering : principles and practice / edited by Jeffrey L. Cleland, Charles S. Craik |
| Pubbl/distr/stampa | New York : Wiley-Liss, c1996 |
| Descrizione fisica | X, 518 p. : ill., ; 25 cm |
| Disciplina | 660.63 |
| Soggetto topico |
Ingegneria biochimica
Proteine Struttura |
| ISBN | 0-471-10354-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNISA-990000315400203316 |
| New York : Wiley-Liss, c1996 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. di Salerno | ||
| ||
Protein engineering
| Protein engineering |
| Pubbl/distr/stampa | Oxford, : IRL, 1986-2003 |
| Descrizione fisica | 16 volumi : ill. ; 28 cm |
| Disciplina | 660.63(Biochimica industriale. Ingegneria biochimica) |
| ISSN | 0269-2139 |
| Formato | Materiale a stampa |
| Livello bibliografico | Periodico |
| Lingua di pubblicazione | eng |
| Record Nr. | UNICAMPANIA-VAN0035895 |
| Oxford, : IRL, 1986-2003 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Vanvitelli | ||
| ||
Protein engineering
| Protein engineering |
| Edizione | [Vol. 1] |
| Descrizione fisica | Mensile. - L'editore varia in: Oxford university. |
| Disciplina | 660.63(Biochimica industriale. Ingegneria biochimica) |
| ISSN | 0269-2139 |
| Formato | Materiale a stampa |
| Livello bibliografico | Periodico |
| Lingua di pubblicazione | eng |
| Record Nr. | UNICAMPANIA-SUN0035895 |
| Materiale a stampa | ||
| Lo trovi qui: Univ. Vanvitelli | ||
|
Protein engineering
| Protein engineering |
| Pubbl/distr/stampa | Oxford, : IRL Press |
| Disciplina |
574.19295
660.63 |
| ISSN | 0269-2139 |
| Formato | Materiale a stampa |
| Livello bibliografico | Periodico |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-990009030190403321 |
| Oxford, : IRL Press | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Protein engineering for therapeutics, Part B / edited by K. Dane Wittrup, Gregory L. Verdine
| Protein engineering for therapeutics, Part B / edited by K. Dane Wittrup, Gregory L. Verdine |
| Pubbl/distr/stampa | Amsterdam [etc.], : Elsevier, 2012 |
| Descrizione fisica | XIV, 348 p. : ill. ; 24 cm |
| Disciplina | 660.63 |
| Collana | Methods in enzymology |
| Soggetto topico | Proteine - Ingegneria biochimica |
| ISBN | 978-0-12-396962-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNISA-990003651110203316 |
| Amsterdam [etc.], : Elsevier, 2012 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. di Salerno | ||
| ||
Protein engineering handbook [[electronic resource]] / edited by Stefan Lutz and Uwe Theo Bornscheuer
| Protein engineering handbook [[electronic resource]] / edited by Stefan Lutz and Uwe Theo Bornscheuer |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Weinheim, [Germany] : , : Wiley-VCH Verlag Gmbh & Co. KGaA, , 2009 |
| Descrizione fisica | 1 online resource (2 volumes, 1021 p.) |
| Disciplina |
615.19
660.63 |
| Soggetto topico | Protein engineering |
| ISBN |
978-3-527-63402-6
3-527-66701-6 1-283-64407-X 3-527-66702-4 3-527-63402-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Protein Engineering Handbook; Contents; Preface; List of Contributors; Volume 1; 1 Guidelines for the Functional Analysis of Engineered and Mutant Enzymes; 1.1 Introduction; 1.2 Steady-State Kinetics; 1.3 Enzyme Assays and the Acquisition of Initial Velocity Data; 1.3.1 Biological Sample Appropriate for Assay; 1.3.2 Enzymatic Assays; 1.3.3 Analysis of Initial Rate Data; 1.3.4 Determination of Functional Catalytic Site Concentrations; 1.4 Steady-State Kinetic Parameters and Their Interpretation; 1.4.1 pH-Dependence of Steady-State Kinetic Parameters; 1.4.2 Analysis of Two-Substrate Enzymes
1.5 Concluding RemarksReferences; 2 Engineering Enantioselectivity in Enzyme-Catalyzed Reactions; 2.1 Introduction; 2.2 Molecular Basis for Enantioselectivity; 2.2.1 Enzymes Stabilize Transition States for Fast-Reacting Enantiomers Better than Slow-Reacting Enantiomers; 2.2.2 The Slow-Reacting Enantiomer Fits by Exchanging Two Substituents; 2.2.3 The Slow Enantiomer Fits by an Umbrella-Like Inversion; 2.3 Qualitative Predictions of Enantioselectivity; 2.3.1 Comparing Substrate Structures Leads to Empirical Rules and Box Models; 2.3.2 Computer Modeling Based on X-Ray Structures of Enzymes 2.3.3 What Is Missing from Current Computer Modeling?2.4 Protein Engineering to Increase or Reverse Enantioselectivity; 2.4.1 Mutations Closer to the Active Site Increase Enantioselectivity More Effectively than Mutations Far from the Active Site; 2.4.2 Reversing Enantioselectivity by Exchanging Locations of Binding Sites or a Catalytic Group; 2.5 Concluding Remarks; References; 3 Mechanism and Catalytic Promiscuity: Emerging Mechanistic Principles for Identifi cation and Manipulation of Catalytically Promiscuous Enzymes; 3.1 Introduction; 3.2 Calculation of Rate Accelerations 3.3 Catalytic Features and Their Propensity for Promiscuity3.3.1 Metal Ions; 3.3.2 Recognition of Transition State Charges: Analysis of the Nature of the Transition State; 3.3.3 Catalytic Dyads and Triads; 3.3.4 General Acid/Base Catalysts in Promiscuous Functional Motifs in Catalytic Superfamilies; 3.4 Steric Effects and Structural Constriction in the Active Site: Product Promiscuity; 3.5 Medium Effects in Enzyme Active Sites; 3.6 Conclusions; References; 4 Φ-Value Analysis of Protein Folding Transition States; 4.1 Introduction; 4.2 Theoretical Principles of Protein Engineering 4.2.1 Overview4.2.2 Basic Concepts; 4.2.3 Theory of Φ-Value Analysis; 4.2.4 Relationship between Φ and Leffler α; 4.2.5 Linear Free-Energy Relationships and Denaturant Concentration; 4.3 Guidelines for the Determination of Accurate Φ-Values; 4.3.1 Buffer Preparation and Selection; 4.3.2 Optimization of Experimental Conditions; 4.3.3 Equilibrium Denaturation Experiments; 4.3.3.1 Practical Considerations; 4.3.3.2 Curve-Fitting; 4.3.4 Kinetic Measurements; 4.3.4.1 Practical Considerations; 4.3.4.2 Curve Fitting; 4.3.4.3 Error Analysis for Chevron Plots; 4.4 Conclusions; Acknowledgments References |
| Record Nr. | UNINA-9910130882603321 |
| Weinheim, [Germany] : , : Wiley-VCH Verlag Gmbh & Co. KGaA, , 2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
