Autore	Elkins Kelly M
Pubbl/distr/stampa	Oxford, : Elsevier, 2012, c2013
Descrizione fisica	1 online resource (225 p.)
Disciplina	572.86 614.1
Soggetto topico	Forensic genetics - Technique DNA fingerprinting Polymerase chain reaction DNA Fingerprinting Forensic Genetics - methods Polymerase Chain Reaction Sequence Analysis, DNA - methods
ISBN	1-283-57266-4 9786613885111 0-12-394833-9
Formato	Materiale a stampa
Livello bibliografico	Monografia
Lingua di pubblicazione	eng
Nota di contenuto	Front Cover; Forensic DNA Biology: A Laboratory Manual; Copyright; Contents; Acknowledgements; About the Author; Welcome; Forensic DNA Biology: An Introduction; BIOLOGY OVERVIEW; RESTRICTION FRAGMENT LENGTH POLYMORPHISMS; POLYMERASE CHAIN REACTION; SHORT TANDEM REPEATS; SINGLE NUCLEOTIDE POLYMORPHISMS; MITOCHONDRIAL DNA; KNOWN VERSUS QUESTIONED SAMPLES; WHY STUDY FORENSIC DNA BIOLOGY?; Laboratory Safety; RULES FOR A SAFE LAB ENVIRONMENT; Reference; Avoiding Contamination Issues: Standard Laboratory Practices; Reference; Chapter 1 - Pipetting; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND PROCEDUREQUESTIONS; GRAPHING THE DATA USING MICROSOFT EXCEL (2003); EQUATIONS; References; Chapter 2 - Serology; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References; Chapter 3 - Sampling Biological Evidence for DNA Extraction; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE: DNA COLLECTION AND PACKAGING; QUESTIONS; References; Chapter 4 - DNA Extraction; OBJECTIVE; SAFETY; MATERIALS; RECIPES FOR BUFFER AND SOLUTION PREPARATION; BACKGROUND; PROCEDURE; QUESTION; References; Chapter 5 - Determination of Quality and Quantity of DNA Using Agarose Gel Electrophoresis OBJECTIVESAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; Reference; Chapter 6 - Determination of DNA Quality and Quantity Using UV-Vis Spectroscopy; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References; Chapter 7 - Determination of DNA Quantity by Fluorescence Spectroscopy; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References; Chapter 8 - Real-Time Polymerase Chain Reaction (PCR) Quantitation of DNA; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References Chapter 9 - Multiplex Polymerase Chain Reaction (PCR) Primer Design (in Silico)OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References; Chapter 10 - Testing Designed Polymerase Chain Reaction (PCR) Primers in Multiplex Reactions; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References; Chapter 11 - Multiplex Polymerase Chain Reaction (PCR) Amplification of Short Tandem Repeat (STR) Loci Using a Commercial Kit; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References Chapter 12 - Capillary Electrophoresis of Short Tandem Repeat (STR) Polymerase Chain Reaction (PCR) Products from a Commercial Multiplex KitOBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTION; References; Chapter 13 - Computing Random Match Probability from DNA Profile Data Using Population Databases; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References; Chapter 14 - Mitochondrial Deoxyribonucleic Acid (mtDNA) Single Nucleotide Polymorphism (SNP) Detection; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References Chapter 15 - Analysis of Deoxyribonucleic Acid (DNA) Sequence Data Using BioEdit
Record Nr.	UNINA-9910790326703321

Autore	Mushegian Arcady R
Pubbl/distr/stampa	Amsterdam ; ; Boston, : Academic Press, c2007
Descrizione fisica	1 online resource (276 p.)
Disciplina	572.8/6 22 572.86
Soggetto topico	Genomics Gene mapping Physiology, Comparative
Soggetto genere / forma	Electronic books.
ISBN	1-281-03696-X 9786611036966 0-08-054609-9
Formato	Materiale a stampa
Livello bibliografico	Monografia
Lingua di pubblicazione	eng
Nota di contenuto	The beginning of computational genomics -- Finding sequence similarities -- Homology: can we get it right? -- Getting ready for the era of comparative genomics: the importance of viruses -- The first fact of comparative genomics: protein sequences are remarkably resilient in evolution -- The second fact of comparative genomics: functional convergence at the molecular level -- Prediction of function and reconstruction of metabolism from genomic data: homology-based approaches -- Prediction of function and reconstruction of metabolism: post-homology approaches -- Structural genomics: what does it tell us about life? -- How many protein families are there? -- Phylogenetic inference and the era of complete genomes -- Two stories about evolution -- Minimal and ancestral genomes -- Comparative genomics and systems biology.
Record Nr.	UNINA-9910458671503321

Pubbl/distr/stampa	Luxembourg : Office for official publications of the European Communities, 2008
Descrizione fisica	508 p. : ill. ; 30 cm + 1 CD-Rom
Disciplina	572.86
Soggetto topico	Genetica molecolare - Progetti - Finanziamenti comunitari
ISBN	978-92-79-08004-3
Formato	Materiale a stampa
Livello bibliografico	Monografia
Lingua di pubblicazione	eng
Record Nr.	UNISA-990003327900203316

Autore	Dale Jeremy (Jeremy W.)
Edizione	[2nd ed.]
Pubbl/distr/stampa	Hoboken, : Wiley, 2008
Descrizione fisica	1 online resource (396 p.)
Disciplina	572.8 572.86 660.65
Altri autori (Persone)	von SchantzMalcolm
Soggetto topico	Cloning, Molecular DNA, Recombinant DNA Genes Genetic Engineering Genomes Genetic engineering Genome Genetic Structures Genetic Techniques Investigative Techniques Genetic Phenomena Nucleic Acids Analytical, Diagnostic and Therapeutic Techniques and Equipment Phenomena and Processes Nucleic Acids, Nucleotides, and Nucleosides Chemicals and Drugs Genetics Biology Health & Biological Sciences
Soggetto genere / forma	Electronic books.
ISBN	0-470-85690-4 1-282-34827-2 0-470-51870-7 0-470-01734-1 9786612348273 1-280-62202-4 9786610622023 0-470-85691-2
Formato	Materiale a stampa
Livello bibliografico	Monografia
Lingua di pubblicazione	eng
Nota di contenuto	""From Genes to Genomes""; ""Contents""; ""Preface""; ""1: Introduction""; ""2: Basic Molecular Biology""; ""2.1 Nucleic acid structure""; ""2.2 What is a gene?""; ""2.3 Information flow: gene expression""; ""2.4 Gene structure and organization""; ""3: How to Clone a Gene""; ""3.1 What is cloning?""; ""3.2 Overview of the procedures""; ""3.3 Gene libraries""; ""3.4 Hybridization""; ""3.5 Polymerase chain reaction""; ""3.6 Extraction and purification of nucleic acids""; ""3.7 Detection and quantitation of nucleic acids""; ""3.8 Gel electrophoresis""; ""4: Cutting and Joining DNA"" ""4.1 Restriction endonucleases""""4.2 Ligation""; ""4.3 Modification of restriction fragment ends""; ""4.4 Other ways of joining DNA molecules""; ""5: Vectors""; ""5.1 Plasmid vectors""; ""5.2 Vectors based on the lambda bacteriophage""; ""5.3 Cosmids""; ""5.4 M13 vectors""; ""5.5 Expression vectors""; ""5.6 Vectors for cloning and expression in eukaryotic cells""; ""5.7 Supervectors: YACs and BACs""; ""5.8 Summary""; ""6: Genomic and cDNA Libraries""; ""6.1 Genomic libraries""; ""6.2 Growing and storing libraries""; ""6.3 cDNA libraries""; ""6.4 Random, arrayed and ordered libraries"" ""7: Finding the Right Clone""""7.1 Screening libraries with gene probes""; ""7.2 Screening expression libraries with antibodies""; ""7.3 Subcloning""; ""7.4 Characterization of plasmid clones""; ""8: Polymerase Chain Reaction""; ""8.1 The PCR reaction""; ""8.2 PCR in practice""; ""8.3 Cloning PCR products""; ""8.4 Long-range PCR""; ""8.5 Reverse-transcription PCR""; ""8.6 Rapid amplification of cDNA ends""; ""8.7 Quantitative PCR""; ""8.8 Applications of PCR""; ""9: Characterization of a Cloned Gene""; ""9.1 DNA sequencing""; ""9.2 Databank entries and annotation""; ""9.3 Sequence analysis"" ""9.4 Sequence comparisons""""9.5 Protein structure""; ""9.6 Confirming gene function""; ""10: Analysis of Gene Expression""; ""10.1 Analysing transcription""; ""10.2 Methods for studying the promoter""; ""10.3 Regulatory elements and DNA-binding proteins""; ""10.4 Translational analysis""; ""11: Products from Native and Manipulated Cloned Genes""; ""11.1 Factors affecting expression of cloned genes""; ""11.2 Expression of cloned genes in bacteria""; ""11.3 Expression in eukaryotic host cells""; ""11.4 Adding tags and signals""; ""11.5 In vitro mutagenesis""; ""11.6 Vaccines"" ""12: Genomic Analysis""""12.1 Genome sequencing""; ""12.2 Analysis and annotation""; ""12.3 Comparing genomes""; ""12.4 Genome browsers""; ""12.5 Relating genes and functions: genetic and physical maps""; ""12.6 Transposon mutagenesis and other screening techniques""; ""12.7 Conclusion""; ""13: Analysis of Genetic Variation""; ""13.1 Single nucleotide polymorphisms""; ""13.2 Larger-scale variations""; ""13.3 Other methods for studying variation""; ""13.4 Human genetic diseases""; ""13.5 Molecular phylogeny""; ""14: Post-genomic Analysis""; ""14.1 Analysing transcription; transcriptomes"" ""14.2 Array-based methods""
Record Nr.	UNINA-9910143510103321

Pubbl/distr/stampa	[New York], : Springer-Verlag, 2000-
Descrizione fisica	1 online resource
Disciplina	572.86
Soggetto topico	Genomes Gene mapping Nucleotide sequence Base Sequence Chromosome Mapping Genome Génomes Cartes chromosomiques Séquence nucléotidique
Soggetto genere / forma	Periodicals.
Soggetto non controllato	Genetics
ISSN	1438-7948
Formato	Materiale a stampa
Livello bibliografico	Periodico
Lingua di pubblicazione	eng
Altri titoli varianti	Functional and integrative genomics
Record Nr.	UNINA-9910139951603321