Microcalorimetry of macromolecules : the physical basis of biological structures / / Peter L. Privalov
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| Autore: |
Privalov Peter L
|
| Titolo: |
Microcalorimetry of macromolecules : the physical basis of biological structures / / Peter L. Privalov
|
| Pubblicazione: | Hoboken, N.J., : Wiley, 2012 |
| Edizione: | 1st ed. |
| Descrizione fisica: | 1 online resource (422 p.) |
| Disciplina: | 547/.7 |
| Soggetto topico: | Macromolecules |
| Macromolecules - Analysis | |
| Calorimeters | |
| Classificazione: | SCI007000 |
| Note generali: | Description based upon print version of record. |
| Nota di bibliografia: | Includes bibliographical references and index. |
| Nota di contenuto: | MICROCALORIMETRY OF MACROMOLECULES; CONTENTS; 1: INTRODUCTION; 2: METHODOLOGY; 2.1. THERMODYNAMIC BASICS OF CALORIMETRY; 2.1.1. Energy; 2.1.2. Enthalpy; 2.1.3. Temperature; 2.1.4. Energy Units; 2.1.5. Heat Capacity; 2.1.6. Kirchhoff's Relation; 2.1.7. Entropy; 2.1.8. Gibbs Free Energy; 2.2. EQUILIBRIUM ANALYSIS; 2.2.1. Two-State Transition; 2.2.2. Derivatives of the Equilibrium Constant; 2.3. AQUEOUS SOLUTIONS; 2.3.1. Specificity of Water as a Solvent; 2.3.2. Acid-Base Equilibrium; 2.3.3. Partial Quantities; 2.4. TRANSFER OF SOLUTES INTO THE AQUEOUS PHASE; 2.4.1. Hydration Effects |
| 2.4.2. Hydrophobic Force2.4.3. Hydration of Polar and Nonpolar Groups; REFERENCES; 3: CALORIMETRY; 3.1. ISOTHERMAL REACTION MICROCALORIMETRY; 3.1.1. The Heat of Mixing Reaction; 3.1.2. Mixing of Reagents in Comparable Volumes; 3.1.3. Isothermal Titration Microcalorimeter; 3.1.4. ITC Experiments; 3.1.5. Analysis of the ITC Data; 3.2. HEAT CAPACITY CALORIMETRY; 3.2.1. Technical Problems; 3.2.2. Differential Scanning Microcalorimeter; 3.2.3. Determination of the Partial Heat Capacity of Solute Molecules; 3.2.4. DSC Experiments | |
| 3.2.5. Determination of the Enthalpy of a Temperature-Induced Process3.2.6. Determination of the van't Hoff Enthalpy; 3.2.7. Multimolecular Two-State Transition; 3.2.8. Analysis of the Complex Heat Capacity Profile; 3.2.9. Correction for Components Refolding; 3.3. PRESSURE PERTURBATION CALORIMETRY; 3.3.1. Heat Effect of Changing Pressure; 3.3.2. Pressure Perturbation Experiment; REFERENCES; 4: MACROMOLECULES; 4.1. EVOLUTION OF THE CONCEPT; 4.2. PROTEINS; 4.2.1. Chemical Structure; 4.2.2. Physical Structure; 4.2.3. Restrictions on the Conformation of Polypeptide Chains | |
| 4.2.4. Regular Conformations of Polypeptide Chain Proteins4.3. HIERARCHY IN PROTEIN STRUCTURE; 4.3.1. Tertiary Structure of Proteins; 4.3.2. Quaternary Structure of Proteins; 4.4. NUCLEIC ACIDS; 4.4.1. Chemical Structure; 4.4.2. Physical Structure; REFERENCES; 5: THE α-HELIX AND α-HELICAL COILED-COIL; 5.1. THE α-HELIX; 5.1.1. Calorimetric Studies of α-Helix Unfolding-Refolding; 5.1.2. Analysis of the Heat Capacity Function; 5.2. α-HELICAL COILED-COILS; 5.2.1. Two-Stranded Coiled-Coils; 5.2.2. Three-Stranded Coiled-Coils; 5.3. α-HELICAL COILED-COIL PROTEINS; 5.3.1. Muscle Proteins | |
| 5.3.2. Myosin Rod5.3.3. Paramyosin; 5.3.4. Tropomyosin; 5.3.5. Leucine Zipper; 5.3.6. Discreteness of the Coiled-Coils; REFERENCES; 6: POLYPROLINE-II COILED-COILS; 6.1. COLLAGENS; 6.1.1. Collagen Superhelix; 6.1.2. Hydrogen Bonds in Collagen; 6.1.3. Stability of Collagens; 6.1.4. Role of Pyrrolidine Rings in Collagen Stabilization; 6.2. CALORIMETRIC STUDIES OF COLLAGENS; 6.2.1. Enthalpy and Entropy of Collagen Melting; 6.2.2. Correlation between Thermodynamic and Structural Characteristics of Collagens; 6.2.3. Role of Water in Maintaining the Collagen Structure | |
| 6.3. THERMODYNAMICS OF COLLAGENS | |
| Sommario/riassunto: | "This is the first textbook on the microcalorimetry of biological molecules. The coverage starts from the basics of thermodynamics (which are unknown for many scientists working in biology), describes evolution of the calorimetric technique, explains how to analyze the calorimetric data, and illustrates these methods with a wide selection of examples. The book provides an essential resource to scientists studying biological molecular structures and the reactions between these structures"-- |
| Titolo autorizzato: | Microcalorimetry of macromolecules |
| ISBN: | 9786613662453 |
| 9781280685514 | |
| 1280685514 | |
| 9781118337493 | |
| 1118337492 | |
| 9781118337509 | |
| 1118337506 | |
| 9781118337479 | |
| 1118337476 | |
| Formato: | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione: | Inglese |
| Record Nr.: | 9910819988403321 |
| Lo trovi qui: | Univ. Federico II |
| Opac: | Controlla la disponibilità qui |