LEADER 05625nam 2200805 a 450 001 9910819988403321 005 20240516134409.0 010 $a9786613662453 010 $a9781280685514 010 $a1280685514 010 $a9781118337493 010 $a1118337492 010 $a9781118337509 010 $a1118337506 010 $a9781118337479 010 $a1118337476 035 $a(CKB)2560000000082886 035 $a(EBL)875923 035 $a(OCoLC)793814212 035 $a(SSID)ssj0000663495 035 $a(PQKBManifestationID)11434601 035 $a(PQKBTitleCode)TC0000663495 035 $a(PQKBWorkID)10603077 035 $a(PQKB)11644798 035 $a(PQKBManifestationID)16032253 035 $a(PQKB)21968398 035 $a(MiAaPQ)EBC875923 035 $a(Au-PeEL)EBL875923 035 $a(CaPaEBR)ebr10580186 035 $a(CaONFJC)MIL366245 035 $a(Perlego)1013722 035 $a(EXLCZ)992560000000082886 100 $a20120515d2012 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aMicrocalorimetry of macromolecules $ethe physical basis of biological structures /$fPeter L. Privalov 205 $a1st ed. 210 $aHoboken, N.J. $cWiley$d2012 215 $a1 online resource (422 p.) 225 0 $aWiley series in protein and peptide science Microcalorimetry of macromolecules 300 $aDescription based upon print version of record. 311 08$a9781118104514 311 08$a111810451X 320 $aIncludes bibliographical references and index. 327 $aMICROCALORIMETRY OF MACROMOLECULES; CONTENTS; 1: INTRODUCTION; 2: METHODOLOGY; 2.1. THERMODYNAMIC BASICS OF CALORIMETRY; 2.1.1. Energy; 2.1.2. Enthalpy; 2.1.3. Temperature; 2.1.4. Energy Units; 2.1.5. Heat Capacity; 2.1.6. Kirchhoff's Relation; 2.1.7. Entropy; 2.1.8. Gibbs Free Energy; 2.2. EQUILIBRIUM ANALYSIS; 2.2.1. Two-State Transition; 2.2.2. Derivatives of the Equilibrium Constant; 2.3. AQUEOUS SOLUTIONS; 2.3.1. Specificity of Water as a Solvent; 2.3.2. Acid-Base Equilibrium; 2.3.3. Partial Quantities; 2.4. TRANSFER OF SOLUTES INTO THE AQUEOUS PHASE; 2.4.1. Hydration Effects 327 $a2.4.2. Hydrophobic Force2.4.3. Hydration of Polar and Nonpolar Groups; REFERENCES; 3: CALORIMETRY; 3.1. ISOTHERMAL REACTION MICROCALORIMETRY; 3.1.1. The Heat of Mixing Reaction; 3.1.2. Mixing of Reagents in Comparable Volumes; 3.1.3. Isothermal Titration Microcalorimeter; 3.1.4. ITC Experiments; 3.1.5. Analysis of the ITC Data; 3.2. HEAT CAPACITY CALORIMETRY; 3.2.1. Technical Problems; 3.2.2. Differential Scanning Microcalorimeter; 3.2.3. Determination of the Partial Heat Capacity of Solute Molecules; 3.2.4. DSC Experiments 327 $a3.2.5. Determination of the Enthalpy of a Temperature-Induced Process3.2.6. Determination of the van't Hoff Enthalpy; 3.2.7. Multimolecular Two-State Transition; 3.2.8. Analysis of the Complex Heat Capacity Profile; 3.2.9. Correction for Components Refolding; 3.3. PRESSURE PERTURBATION CALORIMETRY; 3.3.1. Heat Effect of Changing Pressure; 3.3.2. Pressure Perturbation Experiment; REFERENCES; 4: MACROMOLECULES; 4.1. EVOLUTION OF THE CONCEPT; 4.2. PROTEINS; 4.2.1. Chemical Structure; 4.2.2. Physical Structure; 4.2.3. Restrictions on the Conformation of Polypeptide Chains 327 $a4.2.4. Regular Conformations of Polypeptide Chain Proteins4.3. HIERARCHY IN PROTEIN STRUCTURE; 4.3.1. Tertiary Structure of Proteins; 4.3.2. Quaternary Structure of Proteins; 4.4. NUCLEIC ACIDS; 4.4.1. Chemical Structure; 4.4.2. Physical Structure; REFERENCES; 5: THE ?-HELIX AND ?-HELICAL COILED-COIL; 5.1. THE ?-HELIX; 5.1.1. Calorimetric Studies of ?-Helix Unfolding-Refolding; 5.1.2. Analysis of the Heat Capacity Function; 5.2. ?-HELICAL COILED-COILS; 5.2.1. Two-Stranded Coiled-Coils; 5.2.2. Three-Stranded Coiled-Coils; 5.3. ?-HELICAL COILED-COIL PROTEINS; 5.3.1. Muscle Proteins 327 $a5.3.2. Myosin Rod5.3.3. Paramyosin; 5.3.4. Tropomyosin; 5.3.5. Leucine Zipper; 5.3.6. Discreteness of the Coiled-Coils; REFERENCES; 6: POLYPROLINE-II COILED-COILS; 6.1. COLLAGENS; 6.1.1. Collagen Superhelix; 6.1.2. Hydrogen Bonds in Collagen; 6.1.3. Stability of Collagens; 6.1.4. Role of Pyrrolidine Rings in Collagen Stabilization; 6.2. CALORIMETRIC STUDIES OF COLLAGENS; 6.2.1. Enthalpy and Entropy of Collagen Melting; 6.2.2. Correlation between Thermodynamic and Structural Characteristics of Collagens; 6.2.3. Role of Water in Maintaining the Collagen Structure 327 $a6.3. THERMODYNAMICS OF COLLAGENS 330 $a"This is the first textbook on the microcalorimetry of biological molecules. The coverage starts from the basics of thermodynamics (which are unknown for many scientists working in biology), describes evolution of the calorimetric technique, explains how to analyze the calorimetric data, and illustrates these methods with a wide selection of examples. The book provides an essential resource to scientists studying biological molecular structures and the reactions between these structures"--$cProvided by publisher. 606 $aMacromolecules 606 $aMacromolecules$xAnalysis 606 $aCalorimeters 615 0$aMacromolecules. 615 0$aMacromolecules$xAnalysis. 615 0$aCalorimeters. 676 $a547/.7 686 $aSCI007000$2bisacsh 700 $aPrivalov$b Peter L$01693443 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910819988403321 996 $aMicrocalorimetry of macromolecules$94071236 997 $aUNINA