The Handbook of plant functional genomics : concept and protocols / / edited by Günter Kahl and Khalid Meksem

• Pubblicazione: Weinheim, [Germany] : , : Wiley-VCH Verlag GmbH & Co. KGaA, , 2008; ©2008
• Descrizione fisica: 1 online resource (578 p.)
• Disciplina: 572.862
• Soggetto topico: Plant genomes; Plant genetics
• Persona (resp. second.): KahlGünter; MeksemKhalid
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references at the end of each chapters and index.
• Nota di contenuto: The Handbook of Plant Functional Genomics; Contents; Preface; List of Contributors; I Transcriptome Analysis; A Whole Genome Expression Analysis; 1 Single Cell Expression Profiling: Transcript and Protein Analyses in Isolated Higher Plant Gametes and Zygotes; 1.1 Introduction; 1.2 Microdissection, Cell Isolation; 1.3 In Vitro Fertilization; 1.4 Techniques for Molecular Analyses of Single Cell Types; 1.4.1 Sampling of Single, Living Cells; 1.4.2 Analyses of Gene Expression; 1.4.2.1 Single Cell Gene-by-Gene Analysis; 1.4.2.2 Amplification of Whole cDNA Populations ; 1.4.2.3 Quantification of Transcript Levels1.4.2.4 Library Construction and EST Sequencing; 1.4.2.5 Targeted Approaches Using cDNA Subtraction; 1.4.2.6 Microarray Analyses; 1.5 Analyses of Protein Expression; 1.6 Prospects; References; 2 AFLP-Based RNA Fingerprinting: Novel Variants and Applications; 2.1 Introduction; 2.2 Methods and Protocols; 2.2.1 Theoretical Considerations; 2.2.2 State-of-the-Art cDNA-AFLP Protocol; 2.2.2.1 Isolation of cDNA Fragments; 2.2.2.2 Non-Selective Pre-Amplification; 2.2.2.3 Selective Amplification-Reaction Using (33)P-Labeled Primer and Gel Analysis ; 2.2.2.4 Downstream Analysis2.3 Applications of the Technology; 2.3.1 Fruit Development; 2.3.2 Tuber Development; 2.3.3 Transcript BSA; 2.3.4 Domain Profiling; 2.3.5 VIDISCA; 2.4 Perspectives; References; 3 SuperSAGE: The Most Advanced Transcriptome Technology for Functional Genomics; 3.1 Introduction; 3.2 Methods and Protocols; 3.2.1 Linker Preparation; 3.2.2 RNA Sample; 3.2.3 cDNA Synthesis; 3.2.4 Tag Extraction from cDNA; 3.2.5 Purification of Linker-Tag Fragment; 3.2.6 Ditag Formation and Amplification; 3.2.7 Tag Extraction from Sequence Data; 3.3 Applications of the Technology ; 3.3.1 Interaction Transcriptome3.3.2 Application of SuperSAGE to Non-Model Organisms; 3.3.3 SuperSAGE-Array; 3.3.4 GMAT; 3.4 Perspectives; References; 4 From CAGE to DeepCAGE: High-Throughput Transcription Start Site and Promoter Identification for Gene Network Analysis; 4.1 From Genomes to Transcriptomes; 4.2 Addressing the Complexity of Transcriptomes; 4.3 The Shift From CAGE to DeepCAGE; 4.4 Applications of CAGE and DeepCAGE Libraries; 4.5 Preparation of a DeepCAGE Library; 4.6 CAGE Data Analysis and Genome Mapping Approaches ; 4.7 Expression Profiling: Putting CAGE Tags into a Biological Context4.8 Perspectives; References; 5 Gene Identification Signature-Paired End diTagging (GIS-PET): A Technology for Transcriptome Characterization; 5.1 Introduction; 5.1.1 Microarray Analysis; 5.1.2 cDNA Sequencing, Including EST- and flcDNA-Sequencing; 5.1.3 DNA-Tagging Methods; 5.1.4 Advanced DNA Sequencing Technologies; 5.2 Protocol; 5.2.1 Construction of a GIS-PET flcDNA Library; 5.2.1.1 Reverse-Transcription of mRNA (polyA-RNA) Sample; 5.2.1.2 Oxidation; 5.2.1.3 Biotinylation of RNA Ends ; 5.2.1.4 RNaseONE Selection for Full-Length (-) cDNA/RNA Heteroduplex
• Sommario/riassunto: In this incisive, concise overview of this booming field, the editors -- two of the leading figures in the field with a proven track record -- combine their expertise to provide an invaluable reference on the topic.Following a treatment of transcriptome analysis, the book goes on to discuss replacement and mutation analysis, gene silencing and computational analysis. The whole is rounded off with a look at emerging technologies.Each chapter is accompanied by a concise overview, helping readers to quickly identify topics of interest, while important, carefully selected words and concept
• Titolo autorizzato: The Handbook of plant functional genomics
• ISBN: 1-281-94692-3; 9786611946920; 3-527-62254-3; 3-527-62255-1
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Lingua di pubblicazione: Inglese
• Record Nr.: 9910144124803321
• Lo trovi qui: Univ. Federico II