Methods in animal proteomics / / editors, P. David Eckersall, Phillip D. Whitfield

• Pubblicazione: Chichester, West Sussex ; ; Ames, Iowa, : Wiley-Blackwell, 2011
• Descrizione fisica: 1 online resource (910 p.)
• Disciplina: 636.089; 636.0892398
• Soggetto topico: Proteomics - Methodology; Veterinary medicine - Methodology
• Altri autori: EckersallP. D WhitfieldPhillip D
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Cover; Title page; Copyright page; Contributors; Acknowledgments; Section 1: Exploring Animal Proteomes; 1 An Introduction to Animal Proteomics; 1.1 Proteomics and Animal Systems; 1.2 Exploring Animal Proteomes; 1.3 Applications of Proteomics in Animal Systems; 2 Types of Sample and Experimental Planning; 2.1 Introduction; 2.2 Types of Samples and Their Collection; 2.3 General Preparation of Body Fluids, Cultured Cells in Suspension, and Blood; 2.4 Homogenization, Clarification, and Simplification; 2.5 Determination of Protein Concentration in Proteomic Samples; 2.6 Enzymatic Digestion ; 2.7 Experimental Design: Strategies for Simplification and Enrichment2.8 Specialized Techniques for Sample Preparation; 2.9 Experimental Planning and Design: General Guidelines; 2.10 Biomarker Discovery: Special Considerations for Experimental Planning; 2.11 Concluding Remarks; 3 Protein Separation Strategies; 3.1 Introduction; 3.2 "Classical" 2DE; 3.3 Other Varieties of 2DE; 3.4 Sample Complexity; 3.5 The Influence of Staining; 3.6 "Post-detection"; 3.7 Future Trends; 4 Methods and Approaches to Mass Spectroscopy-Based Protein Identification; 4.1 Introduction; 4.2 MS ; 4.3 Sample Preparation4.4 Protein Identification; 4.5 Linking Mass Spectra with Proteins; 4.6 Validation of MS Data; 4.7 Conclusions; 5 Bioinformatics in Animal Proteomics; 5.1 Introduction; 5.2 Methods; 5.3 Future Trends; 5.4 Sources of Further Information; 6 Comparative Proteomic Approaches; 6.1 Global Study of Proteins; 6.2 Approaches to Characterize the Proteome; 6.3 Quantitative Proteomics; 6.4 Bioinformatics and Biostatistics; 6.5 Future Prospects; 7 Advancing Technologies for Spatial and Temporal Proteomics; 7.1 Introduction; 7.2 Investigating the Spatial Proteome ; 7.3 Temporal Proteomics7.4 Case Study: Spatial and Temporal Profiling in the Chicken; 7.5 Combining Spatial and Temporal Proteomics; 7.6 Recent Developments in Animal Proteomics; 7.7 Future Trends; 7.8 Conclusions; 7.9 Sources of Further Information; Section 2: Applications of Proteomics in Animal Biology; 8 Proteomic Strategies to Investigate Adaptive Processes; 8.1 Introduction; 8.2 Hibernation Physiology and Behavior; 8.3 Sampling Strategy; 8.4 Sample Collection and Storage; 8.5 Design and Execution of a Quantitative Proteomic Experiment; 8.6 Conclusion ; 9 Investigation of Animal Venoms and Toxins9.1 Introduction; 9.2 Natural Bioresources; 9.3 Pharmaceutical Success Stories; 9.4 The Methods in Toxin Sequencing; 9.5 Reducing Complexity by Chromatography; 9.6 Bioassay Considerations; 9.7 Classical Edman; 9.8 Overview of MS Methods for Chemical Prospecting; 9.9 Soft Ionization Methods; 9.10 Common Mass Analyzers for Peptide Analysis; 9.11 Information Obtained from Different MS Systems; 9.12 Cloning Studies; 9.13 New Developments and Future Trends; 9.14 Conclusions; 9.15 Other Sources of Information; 10 Proteomics in Animal Health and Disease ; 10.1 Introduction
• Sommario/riassunto: The study of proteomics provides researchers with a better understanding of disease and physiological processes in animals. Methods in Animal Proteomics will provide animal scientists and veterinarians currently researching these topics in domestic animals a firm foundation in the basics of proteomics methodology, while also reviewing important advances that will be of interest to established researchers in the field. Chapters will provide practical information on a range of topics including protein identification and separation, bioinformatics, and applications to disease and reproduct
• Titolo autorizzato: Methods in animal proteomics
• ISBN: 0-470-96064-7; 0-470-96063-9; 0-470-96066-3
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Lingua di pubblicazione: Inglese
• Record Nr.: 9910830213203321
• Lo trovi qui: Univ. Federico II