05275nam 2200613Ia 450 991083021320332120230725045442.00-470-96064-70-470-96063-90-470-96066-3(CKB)3340000000000199(EBL)1138973(OCoLC)843639680(SSID)ssj0000622296(PQKBManifestationID)11367295(PQKBTitleCode)TC0000622296(PQKBWorkID)10639188(PQKB)11475275(MiAaPQ)EBC1138973(EXLCZ)99334000000000019920110422d2011 uy 0engur|n|---|||||txtrdacontentcrdamediacrrdacarrierMethods in animal proteomics /editors, P. David Eckersall, Phillip D. WhitfieldChichester, West Sussex ;Ames, Iowa Wiley-Blackwell20111 online resource (910 p.)Description based upon print version of record.0-8138-1791-9 Includes bibliographical references and index.Cover; Title page; Copyright page; Contributors; Acknowledgments; Section 1: Exploring Animal Proteomes; 1 An Introduction to Animal Proteomics; 1.1 Proteomics and Animal Systems; 1.2 Exploring Animal Proteomes; 1.3 Applications of Proteomics in Animal Systems; 2 Types of Sample and Experimental Planning; 2.1 Introduction; 2.2 Types of Samples and Their Collection; 2.3 General Preparation of Body Fluids, Cultured Cells in Suspension, and Blood; 2.4 Homogenization, Clarification, and Simplification; 2.5 Determination of Protein Concentration in Proteomic Samples; 2.6 Enzymatic Digestion2.7 Experimental Design: Strategies for Simplification and Enrichment2.8 Specialized Techniques for Sample Preparation; 2.9 Experimental Planning and Design: General Guidelines; 2.10 Biomarker Discovery: Special Considerations for Experimental Planning; 2.11 Concluding Remarks; 3 Protein Separation Strategies; 3.1 Introduction; 3.2 "Classical" 2DE; 3.3 Other Varieties of 2DE; 3.4 Sample Complexity; 3.5 The Influence of Staining; 3.6 "Post-detection"; 3.7 Future Trends; 4 Methods and Approaches to Mass Spectroscopy-Based Protein Identification; 4.1 Introduction; 4.2 MS4.3 Sample Preparation4.4 Protein Identification; 4.5 Linking Mass Spectra with Proteins; 4.6 Validation of MS Data; 4.7 Conclusions; 5 Bioinformatics in Animal Proteomics; 5.1 Introduction; 5.2 Methods; 5.3 Future Trends; 5.4 Sources of Further Information; 6 Comparative Proteomic Approaches; 6.1 Global Study of Proteins; 6.2 Approaches to Characterize the Proteome; 6.3 Quantitative Proteomics; 6.4 Bioinformatics and Biostatistics; 6.5 Future Prospects; 7 Advancing Technologies for Spatial and Temporal Proteomics; 7.1 Introduction; 7.2 Investigating the Spatial Proteome7.3 Temporal Proteomics7.4 Case Study: Spatial and Temporal Profiling in the Chicken; 7.5 Combining Spatial and Temporal Proteomics; 7.6 Recent Developments in Animal Proteomics; 7.7 Future Trends; 7.8 Conclusions; 7.9 Sources of Further Information; Section 2: Applications of Proteomics in Animal Biology; 8 Proteomic Strategies to Investigate Adaptive Processes; 8.1 Introduction; 8.2 Hibernation Physiology and Behavior; 8.3 Sampling Strategy; 8.4 Sample Collection and Storage; 8.5 Design and Execution of a Quantitative Proteomic Experiment; 8.6 Conclusion9 Investigation of Animal Venoms and Toxins9.1 Introduction; 9.2 Natural Bioresources; 9.3 Pharmaceutical Success Stories; 9.4 The Methods in Toxin Sequencing; 9.5 Reducing Complexity by Chromatography; 9.6 Bioassay Considerations; 9.7 Classical Edman; 9.8 Overview of MS Methods for Chemical Prospecting; 9.9 Soft Ionization Methods; 9.10 Common Mass Analyzers for Peptide Analysis; 9.11 Information Obtained from Different MS Systems; 9.12 Cloning Studies; 9.13 New Developments and Future Trends; 9.14 Conclusions; 9.15 Other Sources of Information; 10 Proteomics in Animal Health and Disease10.1 IntroductionThe study of proteomics provides researchers with a better understanding of disease and physiological processes in animals. Methods in Animal Proteomics will provide animal scientists and veterinarians currently researching these topics in domestic animals a firm foundation in the basics of proteomics methodology, while also reviewing important advances that will be of interest to established researchers in the field. Chapters will provide practical information on a range of topics including protein identification and separation, bioinformatics, and applications to disease and reproductProteomicsMethodologyVeterinary medicineMethodologyProteomicsMethodology.Veterinary medicineMethodology.636.089636.0892398Eckersall P. D1670737Whitfield Phillip D1670738MiAaPQMiAaPQMiAaPQBOOK9910830213203321Methods in animal proteomics4032787UNINA