Label-free biosensors : techniques and applications / / edited by Matthew A. Cooper [[electronic resource]]

• Pubblicazione: Cambridge : , : Cambridge University Press, , 2009
• Descrizione fisica: 1 online resource (x, 288 pages) : digital, PDF file(s)
• Disciplina: 610.28/4
• Soggetto topico: Biosensors
• Persona (resp. second.): CooperM. A (Matthew A.)
• Note generali: Title from publisher's bibliographic system (viewed on 05 Oct 2015).
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Label-free optical biosensors : an introduction / Brian T. Cunningham -- Experimental design / Robert Karlsson -- Extracting affinity constants from biosensor binding responses / Rebecca L. Rich and David G. Myszka -- Extracting kinetic rate constants from binding responses / Rebecca L. Rich and David G. Myszka -- Sensor surfaces and receptor deposition / Matthew A. Cooper -- Macromolecular interactions / Francis Markey -- Interactions with membranes and membrane receptors / Matthew A. Cooper -- Application of SPR technology to pharmaceutical relevant drug-receptor interactions / Walter Huber -- High-throughput analysis of biomolecular interactions and cellular responses with resonant waveguide grating biosensors / Ye Fang ... [et al.] -- ITC-derived binding constants : using microgram quantities of protein / Richard K. Brown ... [et al.] -- Electrical impedance technology applied to cell-based assays / Ryan P. McGuinness and Edward Verdonk.
• Sommario/riassunto: Label-free biosensors are devices that use biological or chemical receptors to detect analytes (molecules) in a sample. They give detailed information on the selectivity, affinity, and, in many cases, also the binding kinetics and thermodynamics of an interaction. Although they can be powerful tools in the hands of a skilled user, there is often a lack of knowledge of the best methods for using label-free assays to screen for biologically active molecules and accurately and precisely characterize molecular recognition events. This book reviews both established and newer label-free techniques giving both the expert user and the general reader interested in the technologies and applications behind label-free an insight into the field from expert opinion leaders and practitioners of the technologies. Most importantly, chapters contain worked examples from leaders in the field that take the reader through the basics of experimental design, setup, assay development, and data analysis.
• Titolo autorizzato: Label-free biosensors
• ISBN: 1-107-20037-7; 1-283-33018-0; 9786613330185; 1-139-13469-8; 1-139-12964-3; 1-139-13357-8; 0-511-50474-8; 0-511-62653-3; 0-511-50688-0
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Lingua di pubblicazione: Inglese
• Record Nr.: 9910826813903321
• Lo trovi qui: Univ. Federico II