LEADER 03876nam 22006614a 450 001 9910826813903321 005 20200520144314.0 010 $a1-107-20037-7 010 $a1-283-33018-0 010 $a9786613330185 010 $a1-139-13469-8 010 $a1-139-12964-3 010 $a1-139-13357-8 010 $a0-511-50474-8 010 $a0-511-62653-3 010 $a0-511-50688-0 035 $a(CKB)1000000000719093 035 $a(EBL)424579 035 $a(OCoLC)647697857 035 $a(SSID)ssj0000362273 035 $a(PQKBManifestationID)11263913 035 $a(PQKBTitleCode)TC0000362273 035 $a(PQKBWorkID)10380224 035 $a(PQKB)10849285 035 $a(UkCbUP)CR9780511626531 035 $a(MiAaPQ)EBC424579 035 $a(Au-PeEL)EBL424579 035 $a(CaPaEBR)ebr10289233 035 $a(CaONFJC)MIL333018 035 $a(PPN)261338390 035 $a(EXLCZ)991000000000719093 100 $a20080613d2009 uy 0 101 0 $aeng 135 $aur||||||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 00$aLabel-free biosensors $etechniques and applications /$fedited by Matthew A. Cooper 205 $a1st ed. 210 $aCambridge ;$aNew York $cCambridge University Press$d2009 215 $a1 online resource (x, 288 pages) $cdigital, PDF file(s) 300 $aTitle from publisher's bibliographic system (viewed on 05 Oct 2015). 311 $a0-521-71151-7 311 $a0-521-88453-5 320 $aIncludes bibliographical references and index. 327 $aLabel-free optical biosensors : an introduction / Brian T. Cunningham -- Experimental design / Robert Karlsson -- Extracting affinity constants from biosensor binding responses / Rebecca L. Rich and David G. Myszka -- Extracting kinetic rate constants from binding responses / Rebecca L. Rich and David G. Myszka -- Sensor surfaces and receptor deposition / Matthew A. Cooper -- Macromolecular interactions / Francis Markey -- Interactions with membranes and membrane receptors / Matthew A. Cooper -- Application of SPR technology to pharmaceutical relevant drug-receptor interactions / Walter Huber -- High-throughput analysis of biomolecular interactions and cellular responses with resonant waveguide grating biosensors / Ye Fang ... [et al.] -- ITC-derived binding constants : using microgram quantities of protein / Richard K. Brown ... [et al.] -- Electrical impedance technology applied to cell-based assays / Ryan P. McGuinness and Edward Verdonk. 330 $aLabel-free biosensors are devices that use biological or chemical receptors to detect analytes (molecules) in a sample. They give detailed information on the selectivity, affinity, and, in many cases, also the binding kinetics and thermodynamics of an interaction. Although they can be powerful tools in the hands of a skilled user, there is often a lack of knowledge of the best methods for using label-free assays to screen for biologically active molecules and accurately and precisely characterize molecular recognition events. This book reviews both established and newer label-free techniques giving both the expert user and the general reader interested in the technologies and applications behind label-free an insight into the field from expert opinion leaders and practitioners of the technologies. Most importantly, chapters contain worked examples from leaders in the field that take the reader through the basics of experimental design, setup, assay development, and data analysis. 606 $aBiosensors 615 0$aBiosensors. 676 $a610.28/4 701 $aCooper$b M. A$g(Matthew A.)$01754157 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910826813903321 996 $aLabel-free biosensors$94196749 997 $aUNINA