Phage display [[electronic resource] ] : a practical approach / / edited by Tim Clackson, Henry B. Lowman
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| Titolo: |
Phage display [[electronic resource] ] : a practical approach / / edited by Tim Clackson, Henry B. Lowman
|
| Pubblicazione: | Oxford ; ; New York, : Oxford University Press, c2004 |
| Descrizione fisica: | 1 online resource (357 p.) |
| Disciplina: | 579.26 |
| Soggetto topico: | Bacteriophages |
| Viral proteins | |
| Microbial biotechnology | |
| Soggetto genere / forma: | Electronic books. |
| Altri autori: |
ClacksonTim
LowmanHenry B
|
| Note generali: | Description based upon print version of record. |
| Nota di bibliografia: | Includes bibliographical references and index. |
| Nota di contenuto: | Contents; Protocol list; List of abbreviations; List of contributors; 1 Introduction to phage biology and phage display; 2 Constructing phage display libraries by oligonucleotide-directed mutagenesis; 3 In vitro DNA recombination; 4 Phage selection strategies for improved affinity and specificity of proteins and peptides; 5 Rapid screening of phage displayed protein binding affinities by phage ELISA; 6 Identification of novel ligands for receptors using recombinant peptide libraries; 7 Substrate phage display |
| 8 Protease-based selection of stably folded proteins and protein domains from phage display libraries9 Phage display of zinc fingers and other nucleic acid-binding motifs; 10 In vivo and ex vivo selections using phage-displayed libraries; 11 Screening phage libraries with sera; 12 Interaction cloning using cDNA libraries displayed on phage; 13 Phage antibody libraries; 14 Affinity maturation of phage antibodies; Appendix: Non-standard suppliers; Index; | |
| Sommario/riassunto: | This new book aims to enable researchers to design and undertake all aspects of a phage display project, from designing an experimental strategy and constructing a library to performing selections and analyzing the results. All of the protocols and chapters are extensively cross-referenced, allowing readers to move beyond the specific examples provided in order to customize the procedures for their own protein or selection system of interest. Phage Display is an up-to-date,. comprehensive and integrated experimental guide to the technique, which is essential reading for anyone currently using, |
| Titolo autorizzato: | Phage display |
| ISBN: | 1-280-84529-5 |
| 0-19-151645-7 | |
| Formato: | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione: | Inglese |
| Record Nr.: | 9910458336703321 |
| Lo trovi qui: | Univ. Federico II |
| Opac: | Controlla la disponibilità qui |
Serie:
Practical approach series ; ; 266.