03450nam 2200649Ia 450 991045833670332120200520144314.01-280-84529-50-19-151645-7(CKB)1000000000400418(EBL)422829(OCoLC)476259877(SSID)ssj0000220528(PQKBManifestationID)12067011(PQKBTitleCode)TC0000220528(PQKBWorkID)10142898(PQKB)10107589(MiAaPQ)EBC422829(Au-PeEL)EBL422829(CaPaEBR)ebr10266414(CaONFJC)MIL84529(EXLCZ)99100000000040041820040402d2004 uy 0engur|n|---|||||txtccrPhage display[electronic resource] a practical approach /edited by Tim Clackson, Henry B. LowmanOxford ;New York Oxford University Pressc20041 online resource (357 p.)Practical approach series ;no. 266Description based upon print version of record.0-19-963873-X 0-19-963874-8 Includes bibliographical references and index.Contents; Protocol list; List of abbreviations; List of contributors; 1 Introduction to phage biology and phage display; 2 Constructing phage display libraries by oligonucleotide-directed mutagenesis; 3 In vitro DNA recombination; 4 Phage selection strategies for improved affinity and specificity of proteins and peptides; 5 Rapid screening of phage displayed protein binding affinities by phage ELISA; 6 Identification of novel ligands for receptors using recombinant peptide libraries; 7 Substrate phage display8 Protease-based selection of stably folded proteins and protein domains from phage display libraries9 Phage display of zinc fingers and other nucleic acid-binding motifs; 10 In vivo and ex vivo selections using phage-displayed libraries; 11 Screening phage libraries with sera; 12 Interaction cloning using cDNA libraries displayed on phage; 13 Phage antibody libraries; 14 Affinity maturation of phage antibodies; Appendix: Non-standard suppliers; Index; This new book aims to enable researchers to design and undertake all aspects of a phage display project, from designing an experimental strategy and constructing a library to performing selections and analyzing the results. All of the protocols and chapters are extensively cross-referenced, allowing readers to move beyond the specific examples provided in order to customize the procedures for their own protein or selection system of interest. Phage Display is an up-to-date,. comprehensive and integrated experimental guide to the technique, which is essential reading for anyone currently using,Practical approach series ;266.BacteriophagesViral proteinsMicrobial biotechnologyElectronic books.Bacteriophages.Viral proteins.Microbial biotechnology.579.26Clackson Tim957349Lowman Henry B957350MiAaPQMiAaPQMiAaPQBOOK9910458336703321Phage display2168528UNINA