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Gene: X
| Gene: X |
| Pubbl/distr/stampa | Amsterdam : , : Elsevier B.V., , 2019-2020 |
| Descrizione fisica | 1 online resource |
| Soggetto topico |
Genetic engineering
Molecular cloning Recombinant DNA Genetic Phenomena Molecular Biology |
| Soggetto genere / forma |
Periodicals.
Periodical |
| ISSN | 2590-1583 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Periodico |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910384449503321 |
| Amsterdam : , : Elsevier B.V., , 2019-2020 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
An introduction to recombinant DNA / Alan E. H. Emery
| An introduction to recombinant DNA / Alan E. H. Emery |
| Autore | Emery, Alan E. H. |
| Pubbl/distr/stampa | Chichester [Sussex] : John Wiley & Sons, c1984 |
| Descrizione fisica | x, 223 p. : ill. ; 22 cm |
| Disciplina | 616.042 |
| Collana | A Wiley medical publication |
| Soggetto topico |
Genetic engineering
Medical genetics Pathology, Molecular Recombinant DNA |
| ISBN | 0471903639 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNISALENTO-991003496819707536 |
Emery, Alan E. H.
|
||
| Chichester [Sussex] : John Wiley & Sons, c1984 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. del Salento | ||
| ||
Manipulation and expression of recombinant DNA [[electronic resource] ] : a laboratory manual / / Susan Carson, Dominique Robertson
| Manipulation and expression of recombinant DNA [[electronic resource] ] : a laboratory manual / / Susan Carson, Dominique Robertson |
| Autore | Carson Susan |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | Burlington, MA, : Elsevier Academic Press, c2006 |
| Descrizione fisica | 1 online resource (171 p.) |
| Disciplina | 660.65 |
| Soggetto topico |
Recombinant DNA
Genetic engineering |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-280-62845-6
9786610628452 0-08-045654-5 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cover; Contents; Preface; Acknowledgments; Note to Instructors; Instrumentation; Nomenclature; Introduction; Conceptual Outline for Experiments; Experimental Procedures; Laboratory Safety; General Operating Procedures; Emergency Contact Information; Part I; Manipulation of DNA; Lab Session 1; Getting Oriented; Practicing with Micropipettes; Station Checklist; Micropipetting; Laboratory Exercise; Preparing BSA Dilutions; Performing a Nitrocellulose Spot Test; Lab Session 2; Large-Scale Purification of Plasmid DNA; Introduction; Laboratory Exercises
Alkaline Lysis and Anion Exchange ChromatograhyDNA Quantification; Lab Session 3; Preparation of Expression Vector DNA (pET-41a(+), a GST Fusion Protein Vector); Introduction; Laboratory Exercises; Restriction Digestion of Vector (pET41a) Restriction Enzyme Digestions; Agarose Gel Electrophoresis; Cleaning DNA Using a Spin Column; Lab Session 4; Preparation of Insert DNA (egfp); Introduction; Laboratory Exercises; Restriction Digestion of pEGFP-N1; Isolation of egfp DNA from Agarose; Lab Session 5; Preparation of Transformation-Competent Cells and Control Transformation; Introduction Laboratory Exercises Preparation of Chemically Competent Cells by Calcium Chloride Treatment; Transformation Control; Lab Session 6; DNA Ligation and Transformation of Escherichia coli; Introduction; Laboratory Exercises; Ligations and Ligation Controls; Divalent Cation-Mediated Transformation; Electrophoresis of Ligation Reactions; Part II; Screening Transformants; Lab Session 7; Colony Hybridizations; Lab Session 7A; Interim Laboratory Session; Introduction; Laboratory Exercises; Counting Transformants; Replica Plating; Lab Session 7B; Colony Hybridization: DNA Probe; Introduction Laboratory Exercises Colony Hybridization with an egfp DNA Probe: Part 1; Labeling of DNA Probe by PCR Using Digoxigenin-11-dUTP; Lab Session 7C; Colony Hybridization: Monoclonal Antibody Probe; Introduction; Laboratory Exercise; Colony Hybridization with an a-GFP Monoclonal Antibody Probe: Part 1; Lab Session 8; Completion of Colony Hybridization with DNA Probe; Introduction; Laboratory Exercise; Colony Hybridization with an egfp DNA Probe: Part 2; Lab Session 9; Characterization of Recombinant Clones; Lab Session 9A; Completion of Colony Hybridization with mAB Probe; Introduction Laboratory Exercise Colony Hybridization with an a-GFP monoclonal Antibody Probe: Part 2; Lab Session 9B; PCR Screening; Introduction; Polymerase Chain Reaction; Laboratory Exercise; Polymerase Chain Reaction Screen for Recombinant Clones: Part 1; Lab Session 9C; Visualization of Green Fluorescent Protein: Part 1; Introduction; Laboratory Exercise; Green Fluorescence Assay and Preparation of a Fresh Master Plate; Lab Session 10; Further Characterization of Recombinant Clones; Lab Session 10A:; Interim Laboratory Session; Laboratory Exercise; Inoculate Cultures for Minipreps; Lab Session 10B Analysis of PCR Screen Results |
| Record Nr. | UNINA-9910458479503321 |
|
Carson Susan
|
||
| Burlington, MA, : Elsevier Academic Press, c2006 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Manipulation and expression of recombinant DNA [[electronic resource] ] : a laboratory manual / / Susan Carson, Dominique Robertson
| Manipulation and expression of recombinant DNA [[electronic resource] ] : a laboratory manual / / Susan Carson, Dominique Robertson |
| Autore | Carson Susan |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | Burlington, MA, : Elsevier Academic Press, c2006 |
| Descrizione fisica | 1 online resource (171 p.) |
| Disciplina | 660.65 |
| Soggetto topico |
Recombinant DNA
Genetic engineering |
| ISBN |
1-280-62845-6
9786610628452 0-08-045654-5 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cover; Contents; Preface; Acknowledgments; Note to Instructors; Instrumentation; Nomenclature; Introduction; Conceptual Outline for Experiments; Experimental Procedures; Laboratory Safety; General Operating Procedures; Emergency Contact Information; Part I; Manipulation of DNA; Lab Session 1; Getting Oriented; Practicing with Micropipettes; Station Checklist; Micropipetting; Laboratory Exercise; Preparing BSA Dilutions; Performing a Nitrocellulose Spot Test; Lab Session 2; Large-Scale Purification of Plasmid DNA; Introduction; Laboratory Exercises
Alkaline Lysis and Anion Exchange ChromatograhyDNA Quantification; Lab Session 3; Preparation of Expression Vector DNA (pET-41a(+), a GST Fusion Protein Vector); Introduction; Laboratory Exercises; Restriction Digestion of Vector (pET41a) Restriction Enzyme Digestions; Agarose Gel Electrophoresis; Cleaning DNA Using a Spin Column; Lab Session 4; Preparation of Insert DNA (egfp); Introduction; Laboratory Exercises; Restriction Digestion of pEGFP-N1; Isolation of egfp DNA from Agarose; Lab Session 5; Preparation of Transformation-Competent Cells and Control Transformation; Introduction Laboratory Exercises Preparation of Chemically Competent Cells by Calcium Chloride Treatment; Transformation Control; Lab Session 6; DNA Ligation and Transformation of Escherichia coli; Introduction; Laboratory Exercises; Ligations and Ligation Controls; Divalent Cation-Mediated Transformation; Electrophoresis of Ligation Reactions; Part II; Screening Transformants; Lab Session 7; Colony Hybridizations; Lab Session 7A; Interim Laboratory Session; Introduction; Laboratory Exercises; Counting Transformants; Replica Plating; Lab Session 7B; Colony Hybridization: DNA Probe; Introduction Laboratory Exercises Colony Hybridization with an egfp DNA Probe: Part 1; Labeling of DNA Probe by PCR Using Digoxigenin-11-dUTP; Lab Session 7C; Colony Hybridization: Monoclonal Antibody Probe; Introduction; Laboratory Exercise; Colony Hybridization with an a-GFP Monoclonal Antibody Probe: Part 1; Lab Session 8; Completion of Colony Hybridization with DNA Probe; Introduction; Laboratory Exercise; Colony Hybridization with an egfp DNA Probe: Part 2; Lab Session 9; Characterization of Recombinant Clones; Lab Session 9A; Completion of Colony Hybridization with mAB Probe; Introduction Laboratory Exercise Colony Hybridization with an a-GFP monoclonal Antibody Probe: Part 2; Lab Session 9B; PCR Screening; Introduction; Polymerase Chain Reaction; Laboratory Exercise; Polymerase Chain Reaction Screen for Recombinant Clones: Part 1; Lab Session 9C; Visualization of Green Fluorescent Protein: Part 1; Introduction; Laboratory Exercise; Green Fluorescence Assay and Preparation of a Fresh Master Plate; Lab Session 10; Further Characterization of Recombinant Clones; Lab Session 10A:; Interim Laboratory Session; Laboratory Exercise; Inoculate Cultures for Minipreps; Lab Session 10B Analysis of PCR Screen Results |
| Record Nr. | UNINA-9910784549303321 |
|
Carson Susan
|
||
| Burlington, MA, : Elsevier Academic Press, c2006 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Manipulation and expression of recombinant DNA [[electronic resource] ] : a laboratory manual / / Susan Carson, Dominique Robertson
| Manipulation and expression of recombinant DNA [[electronic resource] ] : a laboratory manual / / Susan Carson, Dominique Robertson |
| Autore | Carson Susan |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | Burlington, MA, : Elsevier Academic Press, c2006 |
| Descrizione fisica | 1 online resource (171 p.) |
| Disciplina | 660.65 |
| Soggetto topico |
Recombinant DNA
Genetic engineering |
| ISBN |
1-280-62845-6
9786610628452 0-08-045654-5 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cover; Contents; Preface; Acknowledgments; Note to Instructors; Instrumentation; Nomenclature; Introduction; Conceptual Outline for Experiments; Experimental Procedures; Laboratory Safety; General Operating Procedures; Emergency Contact Information; Part I; Manipulation of DNA; Lab Session 1; Getting Oriented; Practicing with Micropipettes; Station Checklist; Micropipetting; Laboratory Exercise; Preparing BSA Dilutions; Performing a Nitrocellulose Spot Test; Lab Session 2; Large-Scale Purification of Plasmid DNA; Introduction; Laboratory Exercises
Alkaline Lysis and Anion Exchange ChromatograhyDNA Quantification; Lab Session 3; Preparation of Expression Vector DNA (pET-41a(+), a GST Fusion Protein Vector); Introduction; Laboratory Exercises; Restriction Digestion of Vector (pET41a) Restriction Enzyme Digestions; Agarose Gel Electrophoresis; Cleaning DNA Using a Spin Column; Lab Session 4; Preparation of Insert DNA (egfp); Introduction; Laboratory Exercises; Restriction Digestion of pEGFP-N1; Isolation of egfp DNA from Agarose; Lab Session 5; Preparation of Transformation-Competent Cells and Control Transformation; Introduction Laboratory Exercises Preparation of Chemically Competent Cells by Calcium Chloride Treatment; Transformation Control; Lab Session 6; DNA Ligation and Transformation of Escherichia coli; Introduction; Laboratory Exercises; Ligations and Ligation Controls; Divalent Cation-Mediated Transformation; Electrophoresis of Ligation Reactions; Part II; Screening Transformants; Lab Session 7; Colony Hybridizations; Lab Session 7A; Interim Laboratory Session; Introduction; Laboratory Exercises; Counting Transformants; Replica Plating; Lab Session 7B; Colony Hybridization: DNA Probe; Introduction Laboratory Exercises Colony Hybridization with an egfp DNA Probe: Part 1; Labeling of DNA Probe by PCR Using Digoxigenin-11-dUTP; Lab Session 7C; Colony Hybridization: Monoclonal Antibody Probe; Introduction; Laboratory Exercise; Colony Hybridization with an a-GFP Monoclonal Antibody Probe: Part 1; Lab Session 8; Completion of Colony Hybridization with DNA Probe; Introduction; Laboratory Exercise; Colony Hybridization with an egfp DNA Probe: Part 2; Lab Session 9; Characterization of Recombinant Clones; Lab Session 9A; Completion of Colony Hybridization with mAB Probe; Introduction Laboratory Exercise Colony Hybridization with an a-GFP monoclonal Antibody Probe: Part 2; Lab Session 9B; PCR Screening; Introduction; Polymerase Chain Reaction; Laboratory Exercise; Polymerase Chain Reaction Screen for Recombinant Clones: Part 1; Lab Session 9C; Visualization of Green Fluorescent Protein: Part 1; Introduction; Laboratory Exercise; Green Fluorescence Assay and Preparation of a Fresh Master Plate; Lab Session 10; Further Characterization of Recombinant Clones; Lab Session 10A:; Interim Laboratory Session; Laboratory Exercise; Inoculate Cultures for Minipreps; Lab Session 10B Analysis of PCR Screen Results |
| Record Nr. | UNINA-9910811459303321 |
|
Carson Susan
|
||
| Burlington, MA, : Elsevier Academic Press, c2006 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Recombinant DNA : a short course / James D. Watson, John Tooze, David T. Kurtz
| Recombinant DNA : a short course / James D. Watson, John Tooze, David T. Kurtz |
| Autore | Watson, James D. |
| Pubbl/distr/stampa | New York : Scientific American Books ; distributed by W.H. Freeman, c1983 |
| Descrizione fisica | xiii, 260 p. : col. ill. ; 29 cm. |
| Disciplina | 572.86 |
| Altri autori (Persone) |
Tooze, Johnauthor
Kurtz, David T. |
| Soggetto topico | Recombinant DNA |
| ISBN | 0716714841 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNISALENTO-991003551639707536 |
|
Watson, James D.
|
||
| New York : Scientific American Books ; distributed by W.H. Freeman, c1983 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. del Salento | ||
| ||
The Recombinant DNA Controversy : A Memoir: Science, Politics, and the Public Interest 1974‐1981 / / Donald S. Fredrickson
| The Recombinant DNA Controversy : A Memoir: Science, Politics, and the Public Interest 1974‐1981 / / Donald S. Fredrickson |
| Autore | Fredrickson Donald S. |
| Pubbl/distr/stampa | Washington, D.C. : , : ASM Press, , 2001 |
| Descrizione fisica | 1 online resource (xix, 388 pages) : illustrations |
| Disciplina | 630.274873282 |
| Soggetto topico |
Recombinant DNA
Genetic engineering |
| ISBN | 1-68367-240-2 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Foreword -- LeRoy Walters -- Preface -- Acknowledgments -- Asilomar: the End of the Beginning (1975) -- A Federal Case (1975) -- The Gathering Storm (1975) -- Extending the Seed of Power to the Public (1976) -- Recombinant Ramblings (1976-77) -- The Environmental Impact Statement and the Polyoma Experiment (1976-77) -- Acts of Congress (1977) -- Returning to the Witness Table: the Remainder of the 95th Congress (1977-78) -- The Revising of the Guidelines (1976-78) -- A Major Action (1978-79) -- Winding Down (1979-81) -- Moral -- Epilogue -- Notes -- Appendices -- Index. |
| Altri titoli varianti |
Recombinent DNA Controversy
Recombinant DNA Controversy |
| Record Nr. | UNINA-9910830244003321 |
|
Fredrickson Donald S.
|
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| Washington, D.C. : , : ASM Press, , 2001 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Recombinant DNA technology / / Keya Chaudhuri
| Recombinant DNA technology / / Keya Chaudhuri |
| Autore | Chaudhuri Keya <1952-> |
| Pubbl/distr/stampa | New Delhi, India : , : The Energy and Resources Institute, , 2013 |
| Descrizione fisica | 1 online resource (300 pages) |
| Disciplina | 574.873282 |
| Soggetto topico | Recombinant DNA |
| Soggetto genere / forma | Electronic books. |
| ISBN | 81-7993-617-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910465357703321 |
|
Chaudhuri Keya <1952->
|
||
| New Delhi, India : , : The Energy and Resources Institute, , 2013 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Recombinant DNA technology / / Keya Chaudhuri
| Recombinant DNA technology / / Keya Chaudhuri |
| Autore | Chaudhuri Keya <1952-> |
| Pubbl/distr/stampa | New Delhi, India : , : The Energy and Resources Institute, , 2013 |
| Descrizione fisica | 1 online resource (300 pages) |
| Disciplina | 574.873282 |
| Soggetto topico | Recombinant DNA |
| ISBN | 81-7993-617-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910798116703321 |
|
Chaudhuri Keya <1952->
|
||
| New Delhi, India : , : The Energy and Resources Institute, , 2013 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Recombinant DNA technology / / Keya Chaudhuri
| Recombinant DNA technology / / Keya Chaudhuri |
| Autore | Chaudhuri Keya <1952-> |
| Pubbl/distr/stampa | New Delhi, India : , : The Energy and Resources Institute, , 2013 |
| Descrizione fisica | 1 online resource (300 pages) |
| Disciplina | 574.873282 |
| Soggetto topico | Recombinant DNA |
| ISBN | 81-7993-617-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910810028903321 |
|
Chaudhuri Keya <1952->
|
||
| New Delhi, India : , : The Energy and Resources Institute, , 2013 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Soggetto topico
-
Recombinant DNA
(21)
- Genetic engineering (8)
- ADN recombinant (6)
- Molecular cloning (5)
- ADN (4)