top

  Info

  • Utilizzare la checkbox di selezione a fianco di ciascun documento per attivare le funzionalità di stampa, invio email, download nei formati disponibili del (i) record.

  Info

  • Utilizzare questo link per rimuovere la selezione effettuata.
Gene
Gene
Pubbl/distr/stampa [Amsterdam], : Elsevier Science B.V
Soggetto topico Genetic engineering
Molecular cloning
Recombinant DNA
Genetic recombination
Genetics, Biochemical
Molecular Biology
Recombination, Genetic
Génie génétique
Clonage moléculaire
ADN recombinant
Recombinaison génétique
Soggetto genere / forma Periodical
Periodicals.
Soggetto non controllato GENETICS
CLONING
RECOMBINANT DNA
RECOMBINATION
ISSN 1879-0038
Formato Materiale a stampa
Livello bibliografico Periodico
Lingua di pubblicazione eng
Record Nr. UNINA-9910144170003321
[Amsterdam], : Elsevier Science B.V
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Gene cloning : an introduction / T.A. Brown
Gene cloning : an introduction / T.A. Brown
Autore Brown, Terence Austen
Edizione [3rd edition]
Descrizione fisica 334 pages : illustrations ; 24 cm
Soggetto topico DNA, Recombinant
Molecular cloning
ISBN 9780748740703
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNISALENTO-991004319638507536
Brown, Terence Austen  
Materiale a stampa
Lo trovi qui: Univ. del Salento
Opac: Controlla la disponibilità qui
Gene cloning and DNA analysis : an introduction / / T.A. Brown
Gene cloning and DNA analysis : an introduction / / T.A. Brown
Autore Brown T. A (Terence A.)
Edizione [7th edition.]
Pubbl/distr/stampa Chichester : , : Wiley Blackwell, , 2016
Descrizione fisica 1 online resource (526 p.)
Disciplina 572.8/633
Soggetto topico Molecular cloning
Nucleotide sequence
DNA - Analysis
Soggetto genere / forma Electronic books.
ISBN 1-119-07254-9
1-119-07255-7
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910460927603321
Brown T. A (Terence A.)  
Chichester : , : Wiley Blackwell, , 2016
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Gene cloning and DNA analysis : an introduction / / T. A. Brown
Gene cloning and DNA analysis : an introduction / / T. A. Brown
Autore Brown T. A (Terence A.)
Edizione [Seventh edition.]
Pubbl/distr/stampa Chichester, England : , : Wiley Blackwell, , 2016
Descrizione fisica 1 online resource (526 pages) : illustrations (some color)
Disciplina 572.8/633
Soggetto topico DNA - Analysis
Molecular cloning
Nucleotide sequence
ISBN 9781119072546
1119072549
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910795944403321
Brown T. A (Terence A.)  
Chichester, England : , : Wiley Blackwell, , 2016
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Gene cloning and DNA analysis : an introduction / / T. A. Brown
Gene cloning and DNA analysis : an introduction / / T. A. Brown
Autore Brown T. A (Terence A.)
Edizione [Seventh edition.]
Pubbl/distr/stampa Chichester, England : , : Wiley Blackwell, , 2016
Descrizione fisica 1 online resource (526 pages) : illustrations (some color)
Disciplina 572.8/633
Soggetto topico DNA - Analysis
Molecular cloning
Nucleotide sequence
ISBN 9781119072546
1119072549
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910809135003321
Brown T. A (Terence A.)  
Chichester, England : , : Wiley Blackwell, , 2016
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Gene cloning and DNA analysis [[electronic resource]] : an introduction / / T.A. Brown
Gene cloning and DNA analysis [[electronic resource]] : an introduction / / T.A. Brown
Autore Brown T. A (Terence A.)
Edizione [6th ed.]
Pubbl/distr/stampa Hoboken, : Wiley-Blackwell, 2010
Descrizione fisica 1 online resource (338 p.)
Disciplina 572.8/633
Soggetto topico Molecular cloning
Nucleotide sequence
DNA - Analysis
Soggetto genere / forma Electronic books.
ISBN 1-282-68585-6
9786612685859
1-4443-1861-6
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto GENECLONINGAND DNAANALYSIS; Contents; TO THE SIXTH EDITION Preface to the Sixth Edition; PART IThe Basic Principlesof Gene Cloning andDNA Analysis; Chapter 1Why Gene Cloningand DNA Analysis areImportant; 1.1 The early development of genetics; 1.2 The advent of gene cloning and the polymerasechain reaction; 1.3 What is gene cloning?; 1.4 What is PCR?; 1.5 Why gene cloning and PCR are so important; 1.5.1 Obtaining a pure sample of a gene by cloning; 1.5.2 PCR can also be used to purify a gene; 1.6 How to find your way through this book
Chapter 2Vectors for GeneCloning:Plasmids andBacteriophages2.1 Plasmids; 2.1.1 Size and copy number; 2.1.2 Conjugation and compatibility; 2.1.3 Plasmid classification; 2.1.4 Plasmids in organisms other than bacteria; 2.2 Bacteriophages; 2.2.1 The phage infection cycle; 2.2.2 Lysogenic phages; Gene organization in the λ DNA molecule; The linear and circular forms of λ DNA; M13 - a filamentous phage; 2.2.3 Viruses as cloning vectors for other organisms; Chapter 3Purification of DNAfrom Living Cells; 3.1 Preparation of total cell DNA; 3.1.1 Growing and harvesting a bacterial culture
3.1.2 Preparation of a cell extract3.1.3 Purification of DNA from a cell extract; Removing contaminants by organic extraction and enzyme digestion; Using ion-exchange chromatography to purify DNA from a cell extract; 3.1.4 Concentration of DNA samples; 3.1.5 Measurement of DNA concentration; 3.1.6 Other methods for the preparation of total cell DNA; 3.2 Preparation of plasmid DNA; 3.2.1 Separation on the basis of size; 3.2.2 Separation on the basis of conformation; Alkaline denaturation; Ethidium bromide-caesium chloride density gradient centrifugation; 3.2.3 Plasmid amplification
3.3 Preparation of bacteriophage DNA3.3.1 Growth of cultures to obtain a high λ titer; 3.3.2 Preparation of non-lysogenic λ phages; 3.3.3 Collection of phages from an infected culture; 3.3.4 Purification of DNA from λ phage particles; 3.3.5 Purification of M13 DNA causes few problems; Chapter 4Manipulation ofPurified DNA; 4.1 The range of DNA manipulative enzymes; 4.1.1 Nucleases; 4.1.2 Ligases; 4.1.3 Polymerases; 4.1.4 DNA modifying enzymes; 4.2 Enzymes for cutting DNA - restriction endonucleases; 4.2.1 The discovery and function of restriction endonucleases
4.2.2 Type II restriction endonucleases cut DNA at specificnucleotide sequences4.2.3 Blunt ends and sticky ends; 4.2.4 The frequency of recognition sequences in a DNAmolecule; 4.2.5 Performing a restriction digest in the laboratory; 4.2.6 Analyzing the result of restriction endonucleasecleavage; Separation of molecules by gel electrophoresis; Visualizing DNA molecules in an agarose gel; 4.2.7 Estimation of the sizes of DNA molecules; 4.2.8 Mapping the positions of different restriction sites in aDNA molecule; 4.2.9 Special gel electrophoresis methods for separatinglarger molecules
4.3 Ligation - joining DNA molecules together
Record Nr. UNINA-9910458899903321
Brown T. A (Terence A.)  
Hoboken, : Wiley-Blackwell, 2010
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Gene cloning and DNA analysis [[electronic resource]] : an introduction / / T.A. Brown
Gene cloning and DNA analysis [[electronic resource]] : an introduction / / T.A. Brown
Autore Brown T. A (Terence A.)
Edizione [6th ed.]
Pubbl/distr/stampa Hoboken, : Wiley-Blackwell, 2010
Descrizione fisica 1 online resource (338 p.)
Disciplina 572.8/633
Soggetto topico Molecular cloning
Nucleotide sequence
DNA - Analysis
ISBN 1-282-68585-6
9786612685859
1-4443-1861-6
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto GENECLONINGAND DNAANALYSIS; Contents; TO THE SIXTH EDITION Preface to the Sixth Edition; PART IThe Basic Principlesof Gene Cloning andDNA Analysis; Chapter 1Why Gene Cloningand DNA Analysis areImportant; 1.1 The early development of genetics; 1.2 The advent of gene cloning and the polymerasechain reaction; 1.3 What is gene cloning?; 1.4 What is PCR?; 1.5 Why gene cloning and PCR are so important; 1.5.1 Obtaining a pure sample of a gene by cloning; 1.5.2 PCR can also be used to purify a gene; 1.6 How to find your way through this book
Chapter 2Vectors for GeneCloning:Plasmids andBacteriophages2.1 Plasmids; 2.1.1 Size and copy number; 2.1.2 Conjugation and compatibility; 2.1.3 Plasmid classification; 2.1.4 Plasmids in organisms other than bacteria; 2.2 Bacteriophages; 2.2.1 The phage infection cycle; 2.2.2 Lysogenic phages; Gene organization in the λ DNA molecule; The linear and circular forms of λ DNA; M13 - a filamentous phage; 2.2.3 Viruses as cloning vectors for other organisms; Chapter 3Purification of DNAfrom Living Cells; 3.1 Preparation of total cell DNA; 3.1.1 Growing and harvesting a bacterial culture
3.1.2 Preparation of a cell extract3.1.3 Purification of DNA from a cell extract; Removing contaminants by organic extraction and enzyme digestion; Using ion-exchange chromatography to purify DNA from a cell extract; 3.1.4 Concentration of DNA samples; 3.1.5 Measurement of DNA concentration; 3.1.6 Other methods for the preparation of total cell DNA; 3.2 Preparation of plasmid DNA; 3.2.1 Separation on the basis of size; 3.2.2 Separation on the basis of conformation; Alkaline denaturation; Ethidium bromide-caesium chloride density gradient centrifugation; 3.2.3 Plasmid amplification
3.3 Preparation of bacteriophage DNA3.3.1 Growth of cultures to obtain a high λ titer; 3.3.2 Preparation of non-lysogenic λ phages; 3.3.3 Collection of phages from an infected culture; 3.3.4 Purification of DNA from λ phage particles; 3.3.5 Purification of M13 DNA causes few problems; Chapter 4Manipulation ofPurified DNA; 4.1 The range of DNA manipulative enzymes; 4.1.1 Nucleases; 4.1.2 Ligases; 4.1.3 Polymerases; 4.1.4 DNA modifying enzymes; 4.2 Enzymes for cutting DNA - restriction endonucleases; 4.2.1 The discovery and function of restriction endonucleases
4.2.2 Type II restriction endonucleases cut DNA at specificnucleotide sequences4.2.3 Blunt ends and sticky ends; 4.2.4 The frequency of recognition sequences in a DNAmolecule; 4.2.5 Performing a restriction digest in the laboratory; 4.2.6 Analyzing the result of restriction endonucleasecleavage; Separation of molecules by gel electrophoresis; Visualizing DNA molecules in an agarose gel; 4.2.7 Estimation of the sizes of DNA molecules; 4.2.8 Mapping the positions of different restriction sites in aDNA molecule; 4.2.9 Special gel electrophoresis methods for separatinglarger molecules
4.3 Ligation - joining DNA molecules together
Record Nr. UNINA-9910792331003321
Brown T. A (Terence A.)  
Hoboken, : Wiley-Blackwell, 2010
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Gene cloning and DNA analysis : an introduction / / T.A. Brown
Gene cloning and DNA analysis : an introduction / / T.A. Brown
Autore Brown T. A (Terence A.)
Edizione [6th ed.]
Pubbl/distr/stampa Hoboken, : Wiley-Blackwell, 2010
Descrizione fisica 1 online resource (338 p.)
Disciplina 572.8/633
Soggetto topico Molecular cloning
Nucleotide sequence
DNA - Analysis
ISBN 1-282-68585-6
9786612685859
1-4443-1861-6
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto GENECLONINGAND DNAANALYSIS; Contents; TO THE SIXTH EDITION Preface to the Sixth Edition; PART IThe Basic Principlesof Gene Cloning andDNA Analysis; Chapter 1Why Gene Cloningand DNA Analysis areImportant; 1.1 The early development of genetics; 1.2 The advent of gene cloning and the polymerasechain reaction; 1.3 What is gene cloning?; 1.4 What is PCR?; 1.5 Why gene cloning and PCR are so important; 1.5.1 Obtaining a pure sample of a gene by cloning; 1.5.2 PCR can also be used to purify a gene; 1.6 How to find your way through this book
Chapter 2Vectors for GeneCloning:Plasmids andBacteriophages2.1 Plasmids; 2.1.1 Size and copy number; 2.1.2 Conjugation and compatibility; 2.1.3 Plasmid classification; 2.1.4 Plasmids in organisms other than bacteria; 2.2 Bacteriophages; 2.2.1 The phage infection cycle; 2.2.2 Lysogenic phages; Gene organization in the λ DNA molecule; The linear and circular forms of λ DNA; M13 - a filamentous phage; 2.2.3 Viruses as cloning vectors for other organisms; Chapter 3Purification of DNAfrom Living Cells; 3.1 Preparation of total cell DNA; 3.1.1 Growing and harvesting a bacterial culture
3.1.2 Preparation of a cell extract3.1.3 Purification of DNA from a cell extract; Removing contaminants by organic extraction and enzyme digestion; Using ion-exchange chromatography to purify DNA from a cell extract; 3.1.4 Concentration of DNA samples; 3.1.5 Measurement of DNA concentration; 3.1.6 Other methods for the preparation of total cell DNA; 3.2 Preparation of plasmid DNA; 3.2.1 Separation on the basis of size; 3.2.2 Separation on the basis of conformation; Alkaline denaturation; Ethidium bromide-caesium chloride density gradient centrifugation; 3.2.3 Plasmid amplification
3.3 Preparation of bacteriophage DNA3.3.1 Growth of cultures to obtain a high λ titer; 3.3.2 Preparation of non-lysogenic λ phages; 3.3.3 Collection of phages from an infected culture; 3.3.4 Purification of DNA from λ phage particles; 3.3.5 Purification of M13 DNA causes few problems; Chapter 4Manipulation ofPurified DNA; 4.1 The range of DNA manipulative enzymes; 4.1.1 Nucleases; 4.1.2 Ligases; 4.1.3 Polymerases; 4.1.4 DNA modifying enzymes; 4.2 Enzymes for cutting DNA - restriction endonucleases; 4.2.1 The discovery and function of restriction endonucleases
4.2.2 Type II restriction endonucleases cut DNA at specificnucleotide sequences4.2.3 Blunt ends and sticky ends; 4.2.4 The frequency of recognition sequences in a DNAmolecule; 4.2.5 Performing a restriction digest in the laboratory; 4.2.6 Analyzing the result of restriction endonucleasecleavage; Separation of molecules by gel electrophoresis; Visualizing DNA molecules in an agarose gel; 4.2.7 Estimation of the sizes of DNA molecules; 4.2.8 Mapping the positions of different restriction sites in aDNA molecule; 4.2.9 Special gel electrophoresis methods for separatinglarger molecules
4.3 Ligation - joining DNA molecules together
Record Nr. UNINA-9910809717003321
Brown T. A (Terence A.)  
Hoboken, : Wiley-Blackwell, 2010
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Gene cloning and DNA analysis : an introduction / T. A. Brown
Gene cloning and DNA analysis : an introduction / T. A. Brown
Autore Brown, Terence Austen
Edizione [6th ed.]
Pubbl/distr/stampa Oxford ; Hoboken : Wiley-Blackwell, 2010
Descrizione fisica xvi, 320 p. : col. ill., col. maps ; 26 cm
Disciplina 572.8633
Soggetto topico Molecular cloning
Nucleotide sequence
DNA - Analysis
ISBN 9781405181730
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNISALENTO-991001506899707536
Brown, Terence Austen  
Oxford ; Hoboken : Wiley-Blackwell, 2010
Materiale a stampa
Lo trovi qui: Univ. del Salento
Opac: Controlla la disponibilità qui
Gene: X
Gene: X
Pubbl/distr/stampa Amsterdam : , : Elsevier B.V., , 2019-2020
Descrizione fisica 1 online resource
Soggetto topico Genetic engineering
Molecular cloning
Recombinant DNA
Genetic Phenomena
Molecular Biology
Soggetto genere / forma Periodical
Periodicals.
ISSN 2590-1583
Formato Materiale a stampa
Livello bibliografico Periodico
Lingua di pubblicazione eng
Record Nr. UNINA-9910384449503321
Amsterdam : , : Elsevier B.V., , 2019-2020
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui