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Gene
| Gene |
| Pubbl/distr/stampa | [Amsterdam], : Elsevier Science B.V |
| Soggetto topico |
Genetic engineering
Molecular cloning Recombinant DNA Genetic recombination Genetics, Biochemical Molecular Biology Recombination, Genetic Génie génétique Clonage moléculaire ADN recombinant Recombinaison génétique |
| Soggetto genere / forma |
Periodical
Periodicals. |
| Soggetto non controllato |
GENETICS
CLONING RECOMBINANT DNA RECOMBINATION |
| ISSN | 1879-0038 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Periodico |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910144170003321 |
| [Amsterdam], : Elsevier Science B.V | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Gene cloning : an introduction / T.A. Brown
| Gene cloning : an introduction / T.A. Brown |
| Autore | Brown, Terence Austen |
| Edizione | [3rd edition] |
| Descrizione fisica | 334 pages : illustrations ; 24 cm |
| Soggetto topico |
DNA, Recombinant
Molecular cloning |
| ISBN | 9780748740703 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNISALENTO-991004319638507536 |
Brown, Terence Austen
|
||
| Materiale a stampa | ||
| Lo trovi qui: Univ. del Salento | ||
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Gene cloning and DNA analysis : an introduction / / T.A. Brown
| Gene cloning and DNA analysis : an introduction / / T.A. Brown |
| Autore | Brown T. A (Terence A.) |
| Edizione | [7th edition.] |
| Pubbl/distr/stampa | Chichester : , : Wiley Blackwell, , 2016 |
| Descrizione fisica | 1 online resource (526 p.) |
| Disciplina | 572.8/633 |
| Soggetto topico |
Molecular cloning
Nucleotide sequence DNA - Analysis |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-119-07254-9
1-119-07255-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910460927603321 |
|
Brown T. A (Terence A.)
|
||
| Chichester : , : Wiley Blackwell, , 2016 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Gene cloning and DNA analysis : an introduction / / T. A. Brown
| Gene cloning and DNA analysis : an introduction / / T. A. Brown |
| Autore | Brown T. A (Terence A.) |
| Edizione | [Seventh edition.] |
| Pubbl/distr/stampa | Chichester, England : , : Wiley Blackwell, , 2016 |
| Descrizione fisica | 1 online resource (526 pages) : illustrations (some color) |
| Disciplina | 572.8/633 |
| Soggetto topico |
DNA - Analysis
Molecular cloning Nucleotide sequence |
| ISBN |
9781119072546
1119072549 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910795944403321 |
|
Brown T. A (Terence A.)
|
||
| Chichester, England : , : Wiley Blackwell, , 2016 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Gene cloning and DNA analysis : an introduction / / T. A. Brown
| Gene cloning and DNA analysis : an introduction / / T. A. Brown |
| Autore | Brown T. A (Terence A.) |
| Edizione | [Seventh edition.] |
| Pubbl/distr/stampa | Chichester, England : , : Wiley Blackwell, , 2016 |
| Descrizione fisica | 1 online resource (526 pages) : illustrations (some color) |
| Disciplina | 572.8/633 |
| Soggetto topico |
DNA - Analysis
Molecular cloning Nucleotide sequence |
| ISBN |
9781119072546
1119072549 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910809135003321 |
|
Brown T. A (Terence A.)
|
||
| Chichester, England : , : Wiley Blackwell, , 2016 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Gene cloning and DNA analysis [[electronic resource]] : an introduction / / T.A. Brown
| Gene cloning and DNA analysis [[electronic resource]] : an introduction / / T.A. Brown |
| Autore | Brown T. A (Terence A.) |
| Edizione | [6th ed.] |
| Pubbl/distr/stampa | Hoboken, : Wiley-Blackwell, 2010 |
| Descrizione fisica | 1 online resource (338 p.) |
| Disciplina | 572.8/633 |
| Soggetto topico |
Molecular cloning
Nucleotide sequence DNA - Analysis |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-282-68585-6
9786612685859 1-4443-1861-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
GENECLONINGAND DNAANALYSIS; Contents; TO THE SIXTH EDITION Preface to the Sixth Edition; PART IThe Basic Principlesof Gene Cloning andDNA Analysis; Chapter 1Why Gene Cloningand DNA Analysis areImportant; 1.1 The early development of genetics; 1.2 The advent of gene cloning and the polymerasechain reaction; 1.3 What is gene cloning?; 1.4 What is PCR?; 1.5 Why gene cloning and PCR are so important; 1.5.1 Obtaining a pure sample of a gene by cloning; 1.5.2 PCR can also be used to purify a gene; 1.6 How to find your way through this book
Chapter 2Vectors for GeneCloning:Plasmids andBacteriophages2.1 Plasmids; 2.1.1 Size and copy number; 2.1.2 Conjugation and compatibility; 2.1.3 Plasmid classification; 2.1.4 Plasmids in organisms other than bacteria; 2.2 Bacteriophages; 2.2.1 The phage infection cycle; 2.2.2 Lysogenic phages; Gene organization in the λ DNA molecule; The linear and circular forms of λ DNA; M13 - a filamentous phage; 2.2.3 Viruses as cloning vectors for other organisms; Chapter 3Purification of DNAfrom Living Cells; 3.1 Preparation of total cell DNA; 3.1.1 Growing and harvesting a bacterial culture 3.1.2 Preparation of a cell extract3.1.3 Purification of DNA from a cell extract; Removing contaminants by organic extraction and enzyme digestion; Using ion-exchange chromatography to purify DNA from a cell extract; 3.1.4 Concentration of DNA samples; 3.1.5 Measurement of DNA concentration; 3.1.6 Other methods for the preparation of total cell DNA; 3.2 Preparation of plasmid DNA; 3.2.1 Separation on the basis of size; 3.2.2 Separation on the basis of conformation; Alkaline denaturation; Ethidium bromide-caesium chloride density gradient centrifugation; 3.2.3 Plasmid amplification 3.3 Preparation of bacteriophage DNA3.3.1 Growth of cultures to obtain a high λ titer; 3.3.2 Preparation of non-lysogenic λ phages; 3.3.3 Collection of phages from an infected culture; 3.3.4 Purification of DNA from λ phage particles; 3.3.5 Purification of M13 DNA causes few problems; Chapter 4Manipulation ofPurified DNA; 4.1 The range of DNA manipulative enzymes; 4.1.1 Nucleases; 4.1.2 Ligases; 4.1.3 Polymerases; 4.1.4 DNA modifying enzymes; 4.2 Enzymes for cutting DNA - restriction endonucleases; 4.2.1 The discovery and function of restriction endonucleases 4.2.2 Type II restriction endonucleases cut DNA at specificnucleotide sequences4.2.3 Blunt ends and sticky ends; 4.2.4 The frequency of recognition sequences in a DNAmolecule; 4.2.5 Performing a restriction digest in the laboratory; 4.2.6 Analyzing the result of restriction endonucleasecleavage; Separation of molecules by gel electrophoresis; Visualizing DNA molecules in an agarose gel; 4.2.7 Estimation of the sizes of DNA molecules; 4.2.8 Mapping the positions of different restriction sites in aDNA molecule; 4.2.9 Special gel electrophoresis methods for separatinglarger molecules 4.3 Ligation - joining DNA molecules together |
| Record Nr. | UNINA-9910458899903321 |
|
Brown T. A (Terence A.)
|
||
| Hoboken, : Wiley-Blackwell, 2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Gene cloning and DNA analysis [[electronic resource]] : an introduction / / T.A. Brown
| Gene cloning and DNA analysis [[electronic resource]] : an introduction / / T.A. Brown |
| Autore | Brown T. A (Terence A.) |
| Edizione | [6th ed.] |
| Pubbl/distr/stampa | Hoboken, : Wiley-Blackwell, 2010 |
| Descrizione fisica | 1 online resource (338 p.) |
| Disciplina | 572.8/633 |
| Soggetto topico |
Molecular cloning
Nucleotide sequence DNA - Analysis |
| ISBN |
1-282-68585-6
9786612685859 1-4443-1861-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
GENECLONINGAND DNAANALYSIS; Contents; TO THE SIXTH EDITION Preface to the Sixth Edition; PART IThe Basic Principlesof Gene Cloning andDNA Analysis; Chapter 1Why Gene Cloningand DNA Analysis areImportant; 1.1 The early development of genetics; 1.2 The advent of gene cloning and the polymerasechain reaction; 1.3 What is gene cloning?; 1.4 What is PCR?; 1.5 Why gene cloning and PCR are so important; 1.5.1 Obtaining a pure sample of a gene by cloning; 1.5.2 PCR can also be used to purify a gene; 1.6 How to find your way through this book
Chapter 2Vectors for GeneCloning:Plasmids andBacteriophages2.1 Plasmids; 2.1.1 Size and copy number; 2.1.2 Conjugation and compatibility; 2.1.3 Plasmid classification; 2.1.4 Plasmids in organisms other than bacteria; 2.2 Bacteriophages; 2.2.1 The phage infection cycle; 2.2.2 Lysogenic phages; Gene organization in the λ DNA molecule; The linear and circular forms of λ DNA; M13 - a filamentous phage; 2.2.3 Viruses as cloning vectors for other organisms; Chapter 3Purification of DNAfrom Living Cells; 3.1 Preparation of total cell DNA; 3.1.1 Growing and harvesting a bacterial culture 3.1.2 Preparation of a cell extract3.1.3 Purification of DNA from a cell extract; Removing contaminants by organic extraction and enzyme digestion; Using ion-exchange chromatography to purify DNA from a cell extract; 3.1.4 Concentration of DNA samples; 3.1.5 Measurement of DNA concentration; 3.1.6 Other methods for the preparation of total cell DNA; 3.2 Preparation of plasmid DNA; 3.2.1 Separation on the basis of size; 3.2.2 Separation on the basis of conformation; Alkaline denaturation; Ethidium bromide-caesium chloride density gradient centrifugation; 3.2.3 Plasmid amplification 3.3 Preparation of bacteriophage DNA3.3.1 Growth of cultures to obtain a high λ titer; 3.3.2 Preparation of non-lysogenic λ phages; 3.3.3 Collection of phages from an infected culture; 3.3.4 Purification of DNA from λ phage particles; 3.3.5 Purification of M13 DNA causes few problems; Chapter 4Manipulation ofPurified DNA; 4.1 The range of DNA manipulative enzymes; 4.1.1 Nucleases; 4.1.2 Ligases; 4.1.3 Polymerases; 4.1.4 DNA modifying enzymes; 4.2 Enzymes for cutting DNA - restriction endonucleases; 4.2.1 The discovery and function of restriction endonucleases 4.2.2 Type II restriction endonucleases cut DNA at specificnucleotide sequences4.2.3 Blunt ends and sticky ends; 4.2.4 The frequency of recognition sequences in a DNAmolecule; 4.2.5 Performing a restriction digest in the laboratory; 4.2.6 Analyzing the result of restriction endonucleasecleavage; Separation of molecules by gel electrophoresis; Visualizing DNA molecules in an agarose gel; 4.2.7 Estimation of the sizes of DNA molecules; 4.2.8 Mapping the positions of different restriction sites in aDNA molecule; 4.2.9 Special gel electrophoresis methods for separatinglarger molecules 4.3 Ligation - joining DNA molecules together |
| Record Nr. | UNINA-9910792331003321 |
|
Brown T. A (Terence A.)
|
||
| Hoboken, : Wiley-Blackwell, 2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Gene cloning and DNA analysis : an introduction / / T.A. Brown
| Gene cloning and DNA analysis : an introduction / / T.A. Brown |
| Autore | Brown T. A (Terence A.) |
| Edizione | [6th ed.] |
| Pubbl/distr/stampa | Hoboken, : Wiley-Blackwell, 2010 |
| Descrizione fisica | 1 online resource (338 p.) |
| Disciplina | 572.8/633 |
| Soggetto topico |
Molecular cloning
Nucleotide sequence DNA - Analysis |
| ISBN |
1-282-68585-6
9786612685859 1-4443-1861-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
GENECLONINGAND DNAANALYSIS; Contents; TO THE SIXTH EDITION Preface to the Sixth Edition; PART IThe Basic Principlesof Gene Cloning andDNA Analysis; Chapter 1Why Gene Cloningand DNA Analysis areImportant; 1.1 The early development of genetics; 1.2 The advent of gene cloning and the polymerasechain reaction; 1.3 What is gene cloning?; 1.4 What is PCR?; 1.5 Why gene cloning and PCR are so important; 1.5.1 Obtaining a pure sample of a gene by cloning; 1.5.2 PCR can also be used to purify a gene; 1.6 How to find your way through this book
Chapter 2Vectors for GeneCloning:Plasmids andBacteriophages2.1 Plasmids; 2.1.1 Size and copy number; 2.1.2 Conjugation and compatibility; 2.1.3 Plasmid classification; 2.1.4 Plasmids in organisms other than bacteria; 2.2 Bacteriophages; 2.2.1 The phage infection cycle; 2.2.2 Lysogenic phages; Gene organization in the λ DNA molecule; The linear and circular forms of λ DNA; M13 - a filamentous phage; 2.2.3 Viruses as cloning vectors for other organisms; Chapter 3Purification of DNAfrom Living Cells; 3.1 Preparation of total cell DNA; 3.1.1 Growing and harvesting a bacterial culture 3.1.2 Preparation of a cell extract3.1.3 Purification of DNA from a cell extract; Removing contaminants by organic extraction and enzyme digestion; Using ion-exchange chromatography to purify DNA from a cell extract; 3.1.4 Concentration of DNA samples; 3.1.5 Measurement of DNA concentration; 3.1.6 Other methods for the preparation of total cell DNA; 3.2 Preparation of plasmid DNA; 3.2.1 Separation on the basis of size; 3.2.2 Separation on the basis of conformation; Alkaline denaturation; Ethidium bromide-caesium chloride density gradient centrifugation; 3.2.3 Plasmid amplification 3.3 Preparation of bacteriophage DNA3.3.1 Growth of cultures to obtain a high λ titer; 3.3.2 Preparation of non-lysogenic λ phages; 3.3.3 Collection of phages from an infected culture; 3.3.4 Purification of DNA from λ phage particles; 3.3.5 Purification of M13 DNA causes few problems; Chapter 4Manipulation ofPurified DNA; 4.1 The range of DNA manipulative enzymes; 4.1.1 Nucleases; 4.1.2 Ligases; 4.1.3 Polymerases; 4.1.4 DNA modifying enzymes; 4.2 Enzymes for cutting DNA - restriction endonucleases; 4.2.1 The discovery and function of restriction endonucleases 4.2.2 Type II restriction endonucleases cut DNA at specificnucleotide sequences4.2.3 Blunt ends and sticky ends; 4.2.4 The frequency of recognition sequences in a DNAmolecule; 4.2.5 Performing a restriction digest in the laboratory; 4.2.6 Analyzing the result of restriction endonucleasecleavage; Separation of molecules by gel electrophoresis; Visualizing DNA molecules in an agarose gel; 4.2.7 Estimation of the sizes of DNA molecules; 4.2.8 Mapping the positions of different restriction sites in aDNA molecule; 4.2.9 Special gel electrophoresis methods for separatinglarger molecules 4.3 Ligation - joining DNA molecules together |
| Record Nr. | UNINA-9910809717003321 |
|
Brown T. A (Terence A.)
|
||
| Hoboken, : Wiley-Blackwell, 2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Gene cloning and DNA analysis : an introduction / T. A. Brown
| Gene cloning and DNA analysis : an introduction / T. A. Brown |
| Autore | Brown, Terence Austen |
| Edizione | [6th ed.] |
| Pubbl/distr/stampa | Oxford ; Hoboken : Wiley-Blackwell, 2010 |
| Descrizione fisica | xvi, 320 p. : col. ill., col. maps ; 26 cm |
| Disciplina | 572.8633 |
| Soggetto topico |
Molecular cloning
Nucleotide sequence DNA - Analysis |
| ISBN | 9781405181730 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNISALENTO-991001506899707536 |
|
Brown, Terence Austen
|
||
| Oxford ; Hoboken : Wiley-Blackwell, 2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. del Salento | ||
| ||
Gene: X
| Gene: X |
| Pubbl/distr/stampa | Amsterdam : , : Elsevier B.V., , 2019-2020 |
| Descrizione fisica | 1 online resource |
| Soggetto topico |
Genetic engineering
Molecular cloning Recombinant DNA Genetic Phenomena Molecular Biology |
| Soggetto genere / forma |
Periodical
Periodicals. |
| ISSN | 2590-1583 |
| Formato | Materiale a stampa |
| Livello bibliografico | Periodico |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910384449503321 |
| Amsterdam : , : Elsevier B.V., , 2019-2020 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Soggetto topico
-
Molecular cloning
(30)
- DNA - Analysis (8)
- Nucleotide sequence (8)
- Genetic engineering (5)
- Recombinant DNA (5)