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Genetics [[electronic resource] /] / Karvita B. Ahluwalia
Genetics [[electronic resource] /] / Karvita B. Ahluwalia
Autore Ahluwalia Karvita B
Edizione [2nd ed.]
Pubbl/distr/stampa New Delhi, : New Age International (P) Ltd., Publishers, c2009
Descrizione fisica 1 online resource (468 p.)
Disciplina 576.5
Soggetto topico Genetics
Genetic transcription
Genetic regulation
Human genetics
ISBN 1-282-12904-X
9786612129049
81-224-2880-0
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Cover; Dedicated to My Parents; Preface to the Second Edition; Preface to the First Edition; Contents; Chapter 1. The Pre-Mendelian Era and Mendelism; Chapter 2. Interaction of Genes; Chapter 3. Environment and Gene Expression; Chapter 4. Quantitative or Polygenic Inheritance; Chapter 5. Multiple Alleles; Chapter 6. Cell Division and Chromosome Theory of Inheritance; Chapter 7. Chi-Square Test and Probability; Chapter 8. Linkage, Recombination and Gene Mapping; Chapter 9. Extranuclear Transmission of Traits; Chapter 10. The Genetic Control of Sex; Chapter 11. Variations in Chromosome Number
Chapter 12. Variations in Chromosome structureChapter 13. Nature of the Genetic Material; Chapter 14. The Structure and Replication of DNA; Chapter 15. Gene Transcription and Translation into Proteins; Chapter 16. Genetic Regulation in Prokaryotes and Eukaryotes; Chapter 17. Genetics of Bacteria; Chapter 18. Genetics of Viruses; Chapter 19. The Eukaryotic Chromosome; Chapter 20. Mutations; Chapter 21. Human Genetics; Chapter 22. Fine Structure of The Gene; Chapter 23. Recombinant DNA Technology; Chapter 24. Applications of Recombinant DNA Technology; Chapter 25. Genomics
Evolutionary GeneticsChapter 26. Populations and Gene Frequencies; Chapter 27. Origin of Life-Evolution of Man; Index
Record Nr. UNINA-9910827708903321
Ahluwalia Karvita B  
New Delhi, : New Age International (P) Ltd., Publishers, c2009
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IPS Cells for Modelling and Treatment of Human Diseases / / edited by Michael J. Edel
IPS Cells for Modelling and Treatment of Human Diseases / / edited by Michael J. Edel
Pubbl/distr/stampa Basel : , : MDPI - Multidisciplinary Digital Publishing Institute, , 2015
Descrizione fisica 1 online resource (422 pages) : illustrations
Disciplina 572.8845
Soggetto topico Genetic transcription
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto List of Contributors -- About the Guest Editor -- Preface -- Chapter 1: Neuronal -- Opportunities and Limitations of Modelling Alzheimer's Disease with Induced Pluripotent Stem Cells -- Induced Pluripotent Stem Cells Derived from Alzheimer's Disease Patients: The Promise, the Hope and the Path Ahead -- iPSC-based Models to Unravel Key Pathogenetic Processes underlying Motor Neuron Diseases Development -- Chapter 2: Cardiac -- Bioengineering and Stem Cell Technology in the Treatment of Congenital Heart Disease -- Scalable Electrophysiological Investigation of iPS Cell Derived Cardiomyocytes Obtained by a Lentiviral Purification Strategy -- Clinical Potentials of Cardiomyocytes Derived from Patient-Specific Induced Pluripotent Stem Cells -- Chapter 3: Eye -- iPS Cells for Modelling and Treatment of Retinal Diseases -- Patient-Specific iPSC-Derived RPE for Modeling of Retinal Diseases -- Potential role of Induced Pluripotent Stem Cells (IPSCs) for Cell-Based Therapy of the Ocular Surface -- Chapter 4: Spinal Cord Injury -- The Potential for iPS-Derived Stem Cells as a Therapeutic Strategy for Spinal Cord Injury: Opportunities and Challenges -- The State of Play with iPSCs and Spinal Cord Injury Models -- Chapter 5: Liver -- Potential and Challenges of Induced Pluripotent Stem Cells in Liver Diseases Treatment -- Chapter 6: Muscle -- Myogenic Precursors from iPS Cells for Skeletal Muscle Cell Replacement Therapy -- Chapter 7: Bone -- The Use of Patient-Specific Induced Pluripotent Stem Cells (iPSCs) to Identify Osteoclast Defects in Rare Genetic Bone Disorders -- Chapter 8: Germ Cells -- Human iPS Cell-derived Germ Cells: Current Status and Clinical Potential -- Chapter 9: Genetic Disorders -- Comparing ESC and iPSC-Based Models for Human Genetic Disorders -- Design of a Tumorigenicity Test for Induced Pluripotent Stem Cell (iPSC)-Derived Cell Products -- Concise Review: Methods and Cell Types Used to Generate Down Syndrome Induced Pluripotent Stem Cells -- Chapter 10: Immune Response -- The Possible Future Roles for iPSC-Derived Therapy for Autoimmune Diseases.
Record Nr. UNINA-9910598175403321
Basel : , : MDPI - Multidisciplinary Digital Publishing Institute, , 2015
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IPS cells for modelling and treatment of human diseases / / edited by Michael J. Edel
IPS cells for modelling and treatment of human diseases / / edited by Michael J. Edel
Pubbl/distr/stampa Basel, Switzerland : , : MDPI - Multidisciplinary Digital Publishing Institute, , [2015]
Descrizione fisica 1 online resource (422 pages) : illustrations
Disciplina 572.8845
Soggetto topico Genetic transcription
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto List of Contributors -- About the Guest Editor -- Preface -- Chapter 1: Neuronal -- Opportunities and Limitations of Modelling Alzheimer's Disease with Induced Pluripotent Stem Cells -- Induced Pluripotent Stem Cells Derived from Alzheimer's Disease Patients: The Promise, the Hope and the Path Ahead -- iPSC-based Models to Unravel Key Pathogenetic Processes underlying Motor Neuron Diseases Development -- Chapter 2: Cardiac -- Bioengineering and Stem Cell Technology in the Treatment of Congenital Heart Disease -- Scalable Electrophysiological Investigation of iPS Cell Derived Cardiomyocytes Obtained by a Lentiviral Purification Strategy -- Clinical Potentials of Cardiomyocytes Derived from Patient-Specific Induced Pluripotent Stem Cells -- Chapter 3: Eye -- iPS Cells for Modelling and Treatment of Retinal Diseases -- Patient-Specific iPSC-Derived RPE for Modeling of Retinal Diseases -- Potential role of Induced Pluripotent Stem Cells (IPSCs) for Cell-Based Therapy of the Ocular Surface -- Chapter 4: Spinal Cord Injury -- The Potential for iPS-Derived Stem Cells as a Therapeutic Strategy for Spinal Cord Injury: Opportunities and Challenges -- The State of Play with iPSCs and Spinal Cord Injury Models -- Chapter 5: Liver -- Potential and Challenges of Induced Pluripotent Stem Cells in Liver Diseases Treatment -- Chapter 6: Muscle -- Myogenic Precursors from iPS Cells for Skeletal Muscle Cell Replacement Therapy -- Chapter 7: Bone -- The Use of Patient-Specific Induced Pluripotent Stem Cells (iPSCs) to Identify Osteoclast Defects in Rare Genetic Bone Disorders -- Chapter 8: Germ Cells -- Human iPS Cell-derived Germ Cells: Current Status and Clinical Potential -- Chapter 9: Genetic Disorders -- Comparing ESC and iPSC-Based Models for Human Genetic Disorders -- Design of a Tumorigenicity Test for Induced Pluripotent Stem Cell (iPSC)-Derived Cell Products -- Concise Review: Methods and Cell Types Used to Generate Down Syndrome Induced Pluripotent Stem Cells -- Chapter 10: Immune Response -- The Possible Future Roles for iPSC-Derived Therapy for Autoimmune Diseases.
Record Nr. UNINA-9910688474503321
Basel, Switzerland : , : MDPI - Multidisciplinary Digital Publishing Institute, , [2015]
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Key transitions in animal evolution / editors, Rob Desalle, Bernd Schierwater
Key transitions in animal evolution / editors, Rob Desalle, Bernd Schierwater
Pubbl/distr/stampa Enfield, N. H. : Science Publishers
Descrizione fisica xii, 434 p. : ill. (some col.) ; 24 cm
Disciplina 591.38
Altri autori (Persone) DeSalle, Rob
Schierwater, Bernd
Soggetto topico Phylogeny
Evolution (Biology)
Genetic transcription
ISBN 9781578086955
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNISALENTO-991001221619707536
Enfield, N. H. : Science Publishers
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The Palgrave Handbook of Chinese Language Studies [[electronic resource] /] / edited by Zhengdao Ye
The Palgrave Handbook of Chinese Language Studies [[electronic resource] /] / edited by Zhengdao Ye
Edizione [1st ed. 2022.]
Pubbl/distr/stampa Singapore : , : Springer Nature Singapore : , : Imprint : Palgrave Macmillan, , 2022
Descrizione fisica 1 online resource (1032 pages)
Disciplina 495.1
Soggetto topico Applied linguistics
Communication
Linguistics
Genetic transcription
Intercultural communication
Applied Linguistics
Media and Communication
Gene Transcription
Intercultural Communication
Xinès
Manlleus (Lingüística)
Anglès
Lingüística
Soggetto genere / forma Llibres electrònics
ISBN 9789811609244
9789811609237
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto 1. Research on Language Contact -- 2. Typology Studies in China -- 3. Cognitive Linguistics in China -- 4. Pragmatic Studies in China -- 5. Forensic Linguistics on Chinese -- 6. Sinitic Kinship Semantics -- 7. The Study of Classical Chinese in Europe -- 8. Language situations in contemporary China -- 9. Cantonese Diaspora -- 10. Language Contact: Shanghai Wu -- 11. Sinitic Languages along the Belt and Road -- 12. Hokkien in Diaspora -- 13. ‘Migrating concepts’ in Chinese -- 14. Chinese-English Bilingual Dictionaries -- 15. Chinese and the Search for a Universal Language: from Enlightenment to Contemporary times -- 16. Machine Translation -- 17. Translating Chinese Law -- 18. Chinese children literature in English translation -- 19. Translating films on ethnic minorities into English -- 20. Subtitling in English as cross-cultural mediation -- 21. Subtitling in Chinese -- 22. Technology in Chinese Language Teaching -- 23. Cultural Performance Approach in Chinese language teaching -- 24. Cultural Key Words in Mandarin Chinese -- 25. Mandarin-Speaking Children in Britain -- 26. Chinese as a mother tongue in the context of Global Business English Communication -- 27. Chinese and Singaporean English -- 28. From Kowtow to Gaokao: New Chinese Terms in English language media.
Record Nr. UNINA-9910585971203321
Singapore : , : Springer Nature Singapore : , : Imprint : Palgrave Macmillan, , 2022
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Plant transcription factors : evolutionary, structural and functional aspects / / edited by Daniel H. Gonzalez
Plant transcription factors : evolutionary, structural and functional aspects / / edited by Daniel H. Gonzalez
Pubbl/distr/stampa Amsterdam, Netherlands : , : Elsevier, , 2016
Descrizione fisica 1 online resource (435 p.)
Disciplina 572.8652
Soggetto topico Plant genetic regulation
Plant cell differentiation
Genetic transcription
ISBN 0-12-801127-0
0-12-800854-7
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Cover; Title page; Copyright Page; Contents; List of Contributors; Preface; Section A - General Aspects of Plant Transcription Factors; Chapter 1 - Introduction to Transcription Factor Structure and Function; 1.1 - Introduction: Transcription in eukaryotes; 1.2 - Structure of transcription factors; 1.3 - DNA recognition by transcription factors; 1.4 - DNA-binding domains; 1.5 - Protein-protein interactions; 1.6 - Regulation of transcription factor action; 1.7 - Plant transcription factors; Acknowledgments; References; Chapter 2 - Methods to Study Transcription Factor Structure and Function
2.5.1 - Methods for Protein-Protein Complex Identification2.5.1.1 - Yeast Two-Hybrid Assay (Y2H); 2.5.1.2 - Tandem and One-step Tag-Based Affinity Purification; 2.5.2 - Methods for Verification of Protein-Protein Interactions; 2.5.2.1 - Coimmunoprecipitation; 2.5.2.2 - In Vivo Split Methods; 2.5.2.3 - Resonance Energy Transfer Methods; Acknowledgments; References; Chapter 3 - General Aspects of Plant Transcription Factor Families; 3.1 - Introduction; 3.2 - Overview of the transcription cycle in eukaryotes; 3.2.1 - Core Promoter Elements and General Transcription Factors
3.6 - Plant-specific TF families
Record Nr. UNINA-9910797500603321
Amsterdam, Netherlands : , : Elsevier, , 2016
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Plant transcription factors : evolutionary, structural and functional aspects / / edited by Daniel H. Gonzalez
Plant transcription factors : evolutionary, structural and functional aspects / / edited by Daniel H. Gonzalez
Pubbl/distr/stampa Amsterdam, Netherlands : , : Elsevier, , 2016
Descrizione fisica 1 online resource (435 p.)
Disciplina 572.8652
Soggetto topico Plant genetic regulation
Plant cell differentiation
Genetic transcription
ISBN 0-12-801127-0
0-12-800854-7
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Cover; Title page; Copyright Page; Contents; List of Contributors; Preface; Section A - General Aspects of Plant Transcription Factors; Chapter 1 - Introduction to Transcription Factor Structure and Function; 1.1 - Introduction: Transcription in eukaryotes; 1.2 - Structure of transcription factors; 1.3 - DNA recognition by transcription factors; 1.4 - DNA-binding domains; 1.5 - Protein-protein interactions; 1.6 - Regulation of transcription factor action; 1.7 - Plant transcription factors; Acknowledgments; References; Chapter 2 - Methods to Study Transcription Factor Structure and Function
2.5.1 - Methods for Protein-Protein Complex Identification2.5.1.1 - Yeast Two-Hybrid Assay (Y2H); 2.5.1.2 - Tandem and One-step Tag-Based Affinity Purification; 2.5.2 - Methods for Verification of Protein-Protein Interactions; 2.5.2.1 - Coimmunoprecipitation; 2.5.2.2 - In Vivo Split Methods; 2.5.2.3 - Resonance Energy Transfer Methods; Acknowledgments; References; Chapter 3 - General Aspects of Plant Transcription Factor Families; 3.1 - Introduction; 3.2 - Overview of the transcription cycle in eukaryotes; 3.2.1 - Core Promoter Elements and General Transcription Factors
3.6 - Plant-specific TF families
Record Nr. UNINA-9910811445403321
Amsterdam, Netherlands : , : Elsevier, , 2016
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RNA and DNA editing [[electronic resource] ] : molecular mechanisms and their integration into biological systems / / edited by Harold C. Smith
RNA and DNA editing [[electronic resource] ] : molecular mechanisms and their integration into biological systems / / edited by Harold C. Smith
Pubbl/distr/stampa Hoboken, N.J., : Wiley-Interscience, c2008
Descrizione fisica 1 online resource (464 p.)
Disciplina 572.8/8
Altri autori (Persone) SmithHarold C
Soggetto topico RNA editing
DNA
Nucleotide sequence
Genetic transcription
ISBN 1-281-23753-1
9786611237530
0-470-26226-5
0-470-26225-7
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto RNA AND DNA EDITING; CONTENTS; PREFACE; ACKNOWLEDGMENTS; CONTRIBUTORS; PART I DIVERSIFICATION OF THE PROTEOME THROUGH RNA AND DNA EDITING; CHAPTER 1 DIVERSIFYING EXON CODE THROUGH A-TO-I RNA EDITING; 1.1 Introduction and Background; 1.1.1 Initial Discovery and Context of A-to-I RNA Editing and ADARs; 1.1.2 Important Cases of Recoding by A-to-I Modification in Pre-mRNA; 1.1.3 Cis-Acting Features for A-to-I Editing; 1.1.4 Properties of the A-to-I Editing Machinery; 1.2 Main Questions in the Field and Approaches; 1.2.1 Biochemical Versus Computational Approaches; 1.2.2 Editing of miRNA Sequences
1.3 Future Directions: Evolution of Editing Sites and MachineryReferences; CHAPTER 2 ANTIBODY GENE DIVERSIFICATION BY AID-CATALYZED DNA EDITING; 2.1 Introduction; 2.2 Before AID; 2.2.1 Without DNA (Darkness) and with DNA (Light); 2.2.2 Prominent Early Models for Antibody Diversification; 2.2.3 How Protein Sequencing Technology Enabled an Understanding of Antibody Diversity; 2.2.4 Somatic DNA Rearrangements Underpin V(D)J Joining and Create the Primary Antibody Repertoire; 2.2.5 Additional Antibody Diversity by Somatic Hypermutation (and Gene Conversion in Some Animals)
2.2.6 Altering Antibody Function by Class Switch Recombination (Isotype Switching)2.3 After AID; 2.3.1 A Novel Deaminase Is Required for CSR, SHM, and IGC; 2.3.2 AID Is a DNA Cytosine Deaminase that Directly Triggers Antibody Diversification; 2.3.3 The Importance of Uracil Bases in DNA In Vivo; 2.3.4 Processing of AID-induced Lesions: The Molecular Mechanism of Somatic-Hypermutation; 2.3.5 Processing of AID-induced Lesions: The Molecular Mechanism of Immunoglobulin Gene Conversion; 2.3.6 Processing of AID-induced Lesions: The Molecular Mechanism of Class Switch Recombination
2.4 Hot Areas and Speculations2.4.1 Immunodeficiency Syndromes Caused by Defects in AID-Mediated Ig Gene Diversification; 2.4.2 Regulating the DNA Mutator Activity of AID; 2.4.3 Misregulation of AID and Cancer; 2.4.4 AID Is But One Member of a Much Larger Family of Polynucleotide Deaminases; 2.5 Conclusions; Acknowledgments; References; CHAPTER 3 PROTEIN-PROTEIN AND RNA-PROTEIN INTERACTIONS IN U-INSERTION/DELETION RNA EDITING COMPLEXES; 3.1 A Bizarre Phenomenon and its Raison D'être; 3.2 The Catalytic Mechanism and Machinery
3.3 Extent of U-Insertion/Deletion RNA Editing in Trypanosoma and Leishmania Species3.4 Functional Studies of Editing Complex Subunits; 3.4.1 REN1, REN2, and MP67. Endonuclease Homologs; 3.4.2 REX1 and REX2. Exonuclease Homologs; 3.4.3 RET2. TUTase; 3.4.4 REL1 and REL2. Ligase Homologs; 3.4.5 MP81, MP63, MP42, MP46, MP44, MP24, MP18. Structural Components; 3.5 RNA-Protein Interactions: Isolated Subunits and Assembled Editing Complexes; 3.5.1 MP42; 3.5.2 MP24; 3.5.3 RNA-Protein Interactions in Assembled Editing Complexes; 3.6 Concluding Remarks; Acknowledgments; References
CHAPTER 4 MACHINERY OF RNA EDITING IN PLANT ORGANELLES
Record Nr. UNINA-9910145699603321
Hoboken, N.J., : Wiley-Interscience, c2008
Materiale a stampa
Lo trovi qui: Univ. Federico II
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RNA and DNA editing [[electronic resource] ] : molecular mechanisms and their integration into biological systems / / edited by Harold C. Smith
RNA and DNA editing [[electronic resource] ] : molecular mechanisms and their integration into biological systems / / edited by Harold C. Smith
Pubbl/distr/stampa Hoboken, N.J., : Wiley-Interscience, c2008
Descrizione fisica 1 online resource (464 p.)
Disciplina 572.8/8
Altri autori (Persone) SmithHarold C
Soggetto topico RNA editing
DNA
Nucleotide sequence
Genetic transcription
ISBN 1-281-23753-1
9786611237530
0-470-26226-5
0-470-26225-7
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto RNA AND DNA EDITING; CONTENTS; PREFACE; ACKNOWLEDGMENTS; CONTRIBUTORS; PART I DIVERSIFICATION OF THE PROTEOME THROUGH RNA AND DNA EDITING; CHAPTER 1 DIVERSIFYING EXON CODE THROUGH A-TO-I RNA EDITING; 1.1 Introduction and Background; 1.1.1 Initial Discovery and Context of A-to-I RNA Editing and ADARs; 1.1.2 Important Cases of Recoding by A-to-I Modification in Pre-mRNA; 1.1.3 Cis-Acting Features for A-to-I Editing; 1.1.4 Properties of the A-to-I Editing Machinery; 1.2 Main Questions in the Field and Approaches; 1.2.1 Biochemical Versus Computational Approaches; 1.2.2 Editing of miRNA Sequences
1.3 Future Directions: Evolution of Editing Sites and MachineryReferences; CHAPTER 2 ANTIBODY GENE DIVERSIFICATION BY AID-CATALYZED DNA EDITING; 2.1 Introduction; 2.2 Before AID; 2.2.1 Without DNA (Darkness) and with DNA (Light); 2.2.2 Prominent Early Models for Antibody Diversification; 2.2.3 How Protein Sequencing Technology Enabled an Understanding of Antibody Diversity; 2.2.4 Somatic DNA Rearrangements Underpin V(D)J Joining and Create the Primary Antibody Repertoire; 2.2.5 Additional Antibody Diversity by Somatic Hypermutation (and Gene Conversion in Some Animals)
2.2.6 Altering Antibody Function by Class Switch Recombination (Isotype Switching)2.3 After AID; 2.3.1 A Novel Deaminase Is Required for CSR, SHM, and IGC; 2.3.2 AID Is a DNA Cytosine Deaminase that Directly Triggers Antibody Diversification; 2.3.3 The Importance of Uracil Bases in DNA In Vivo; 2.3.4 Processing of AID-induced Lesions: The Molecular Mechanism of Somatic-Hypermutation; 2.3.5 Processing of AID-induced Lesions: The Molecular Mechanism of Immunoglobulin Gene Conversion; 2.3.6 Processing of AID-induced Lesions: The Molecular Mechanism of Class Switch Recombination
2.4 Hot Areas and Speculations2.4.1 Immunodeficiency Syndromes Caused by Defects in AID-Mediated Ig Gene Diversification; 2.4.2 Regulating the DNA Mutator Activity of AID; 2.4.3 Misregulation of AID and Cancer; 2.4.4 AID Is But One Member of a Much Larger Family of Polynucleotide Deaminases; 2.5 Conclusions; Acknowledgments; References; CHAPTER 3 PROTEIN-PROTEIN AND RNA-PROTEIN INTERACTIONS IN U-INSERTION/DELETION RNA EDITING COMPLEXES; 3.1 A Bizarre Phenomenon and its Raison D'être; 3.2 The Catalytic Mechanism and Machinery
3.3 Extent of U-Insertion/Deletion RNA Editing in Trypanosoma and Leishmania Species3.4 Functional Studies of Editing Complex Subunits; 3.4.1 REN1, REN2, and MP67. Endonuclease Homologs; 3.4.2 REX1 and REX2. Exonuclease Homologs; 3.4.3 RET2. TUTase; 3.4.4 REL1 and REL2. Ligase Homologs; 3.4.5 MP81, MP63, MP42, MP46, MP44, MP24, MP18. Structural Components; 3.5 RNA-Protein Interactions: Isolated Subunits and Assembled Editing Complexes; 3.5.1 MP42; 3.5.2 MP24; 3.5.3 RNA-Protein Interactions in Assembled Editing Complexes; 3.6 Concluding Remarks; Acknowledgments; References
CHAPTER 4 MACHINERY OF RNA EDITING IN PLANT ORGANELLES
Record Nr. UNINA-9910807054603321
Hoboken, N.J., : Wiley-Interscience, c2008
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RNA interference in practice [[electronic resource] /] / Ute Schepers
RNA interference in practice [[electronic resource] /] / Ute Schepers
Autore Schepers Ute
Pubbl/distr/stampa Weinheim, : Wiley-VCH, 2005
Descrizione fisica 1 online resource (338 p.)
Disciplina 572.8845
Soggetto topico RNA editing
Genetic transcription
Soggetto genere / forma Electronic books.
ISBN 1-280-51971-1
9786610519712
3-527-60439-1
3-527-60437-5
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto RNA Interference in Practice; Preface; Contents; 1 Introduction: RNA interference, the ""Breakthrough of the Year 2002""; 1.1 RNAi as a Tool for Functional Genomics; 1.2 Mechanism of RNAi; 1.3 Dicer - the Initiator to "Dice" the dsRNA?; 1.4 miRNAs versus siRNAs: Two Classes of Small RNAs Using the RNAi Pathway?; 1.5 RISC - the Effector to "Slice" the mRNA?; 1.6 Are RNA-Dependent RNA Polymerases (RdRps) Responsible for the Catalytic Nature of RNAi?; 1.7 Is RNAi Involved in the Regulation of Gene Expression?; 1.8 RNAi in Mammals; 1.9 Practical Approaches; 1.10 References
2 RNAi in Caenorhabditis elegans2.1 Introduction; 2.2 Application of RNAi in C. elegans; 2.3 Target Sequence Evaluation; 2.4 dsRNA Synthesis; 2.4.2 Generation of the DNA Template; 2.4.2.1 Plasmid Templates; 2.4.2.2 DNA Templates Derived by PCR/RT-PCR; 2.4.2 In-vitro dsRNA Transcription; 2.5 Delivery of dsRNA; 2.5.1 General Information on the C. elegans Anatomy; 2.5.2 C. elegans Strains for Silencing; 2.5.3 Culturing the Worms; 2.5.4 Microinjection Protocol; 2.5.5 Soaking Protocol; 2.5.6 RNAi Feeding Protocol; 2.5.7 DNA Templates for dsRNA Expression in Feeding E. coli
2.5.8 DNA Templates for Hairpin RNA Expression2.5.8.1 C. elegans Promoters; 2.5.8.2 Inverted Repeat Constructs; 2.6 Mounting Animals for Microscopy; 2.7 Genome Wide Screens; 2.7.1 C. elegans RNAi Library; 2.8 Selected Literature on C. elegans Research; 2.9 Useful C. elegans webpages; 2.10 References; 3 RNAi in Drosophila; 3.1 Introduction; 3.2 Application of RNAi in Drosophila; 3.2.1 dsRNA from Linear DNA Templates; 3.2.2 dsRNA from Inverted Repeat DNA; 3.2.3 Inducible Expression in Drosophila Cell Lines; 3.2.4 Limitations; 3.3 dsRNA Synthesis; 3.3.1 In-vitro dsRNA Transcription
3.3.2 Inverted Repeat DNA3.4 Injections; 3.4.1 Injection Services; 3.4.2 Injection Method; 3.4.3 DsRNA or Inverted Repeat DNA Preparation; 3.4.4 Embryo Collection; 3.5 Cell Lines; 3.6 Protocols; 3.6.1 Thawing and Maintenance of S2 Cells; 3.6.2 Freezing Protocol; 3.7 RNAi in S2 Cells; 3.7.1 dsRNA Transfection Using the Calcium Phosphate Method; 3.7.2 dsRNA Soaking of S2 Cells; 3.8 High-Throughput Screens; 3.8.1 Drosophila RNAi Library; 3.9 Useful Webpages for Drosophila Research; 3.10 Books and Literature on Drosophila; 3.11 References; 4 RNAi in Mammals; 4.1 Introduction
4.2 Transient RNAi in Cell Culture4.2.1 Chemical Synthesis and Modifications of siRNAs; 4.2.1.1 Advantages; 4.2.1.2 Limitations; 4.2.2 Custom Synthesis of siRNA Oligos; 4.2.3 siRNA Design Rules; 4.2.3.1 siRNA Strand Bias and Off-Gene Targeting; 4.2.3.2 Improvements in siRNA Stability; 4.2.3.3 siRNA Design: Novel Modifications of the "Tuschl Rules"; 4.2.3.4 Homology Search by BLAST, FASTA, or Smith-Waterman Algorithm; 4.2.3.5 Troubleshooting; 4.2.3.6 siRNA Design Programs and Algorithms; 4.2.3.7 Preparation of siRNA Duplexes; 4.2.4 Enzymatic Synthesis of siRNAs
4.2.4.1 Designing DNA Oligonucleotides
Record Nr. UNINA-9910144557603321
Schepers Ute  
Weinheim, : Wiley-VCH, 2005
Materiale a stampa
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