Genetics [[electronic resource] /] / Karvita B. Ahluwalia |
Autore | Ahluwalia Karvita B |
Edizione | [2nd ed.] |
Pubbl/distr/stampa | New Delhi, : New Age International (P) Ltd., Publishers, c2009 |
Descrizione fisica | 1 online resource (468 p.) |
Disciplina | 576.5 |
Soggetto topico |
Genetics
Genetic transcription Genetic regulation Human genetics |
ISBN |
1-282-12904-X
9786612129049 81-224-2880-0 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Cover; Dedicated to My Parents; Preface to the Second Edition; Preface to the First Edition; Contents; Chapter 1. The Pre-Mendelian Era and Mendelism; Chapter 2. Interaction of Genes; Chapter 3. Environment and Gene Expression; Chapter 4. Quantitative or Polygenic Inheritance; Chapter 5. Multiple Alleles; Chapter 6. Cell Division and Chromosome Theory of Inheritance; Chapter 7. Chi-Square Test and Probability; Chapter 8. Linkage, Recombination and Gene Mapping; Chapter 9. Extranuclear Transmission of Traits; Chapter 10. The Genetic Control of Sex; Chapter 11. Variations in Chromosome Number
Chapter 12. Variations in Chromosome structureChapter 13. Nature of the Genetic Material; Chapter 14. The Structure and Replication of DNA; Chapter 15. Gene Transcription and Translation into Proteins; Chapter 16. Genetic Regulation in Prokaryotes and Eukaryotes; Chapter 17. Genetics of Bacteria; Chapter 18. Genetics of Viruses; Chapter 19. The Eukaryotic Chromosome; Chapter 20. Mutations; Chapter 21. Human Genetics; Chapter 22. Fine Structure of The Gene; Chapter 23. Recombinant DNA Technology; Chapter 24. Applications of Recombinant DNA Technology; Chapter 25. Genomics Evolutionary GeneticsChapter 26. Populations and Gene Frequencies; Chapter 27. Origin of Life-Evolution of Man; Index |
Record Nr. | UNINA-9910827708903321 |
Ahluwalia Karvita B | ||
New Delhi, : New Age International (P) Ltd., Publishers, c2009 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
IPS Cells for Modelling and Treatment of Human Diseases / / edited by Michael J. Edel |
Pubbl/distr/stampa | Basel : , : MDPI - Multidisciplinary Digital Publishing Institute, , 2015 |
Descrizione fisica | 1 online resource (422 pages) : illustrations |
Disciplina | 572.8845 |
Soggetto topico | Genetic transcription |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto | List of Contributors -- About the Guest Editor -- Preface -- Chapter 1: Neuronal -- Opportunities and Limitations of Modelling Alzheimer's Disease with Induced Pluripotent Stem Cells -- Induced Pluripotent Stem Cells Derived from Alzheimer's Disease Patients: The Promise, the Hope and the Path Ahead -- iPSC-based Models to Unravel Key Pathogenetic Processes underlying Motor Neuron Diseases Development -- Chapter 2: Cardiac -- Bioengineering and Stem Cell Technology in the Treatment of Congenital Heart Disease -- Scalable Electrophysiological Investigation of iPS Cell Derived Cardiomyocytes Obtained by a Lentiviral Purification Strategy -- Clinical Potentials of Cardiomyocytes Derived from Patient-Specific Induced Pluripotent Stem Cells -- Chapter 3: Eye -- iPS Cells for Modelling and Treatment of Retinal Diseases -- Patient-Specific iPSC-Derived RPE for Modeling of Retinal Diseases -- Potential role of Induced Pluripotent Stem Cells (IPSCs) for Cell-Based Therapy of the Ocular Surface -- Chapter 4: Spinal Cord Injury -- The Potential for iPS-Derived Stem Cells as a Therapeutic Strategy for Spinal Cord Injury: Opportunities and Challenges -- The State of Play with iPSCs and Spinal Cord Injury Models -- Chapter 5: Liver -- Potential and Challenges of Induced Pluripotent Stem Cells in Liver Diseases Treatment -- Chapter 6: Muscle -- Myogenic Precursors from iPS Cells for Skeletal Muscle Cell Replacement Therapy -- Chapter 7: Bone -- The Use of Patient-Specific Induced Pluripotent Stem Cells (iPSCs) to Identify Osteoclast Defects in Rare Genetic Bone Disorders -- Chapter 8: Germ Cells -- Human iPS Cell-derived Germ Cells: Current Status and Clinical Potential -- Chapter 9: Genetic Disorders -- Comparing ESC and iPSC-Based Models for Human Genetic Disorders -- Design of a Tumorigenicity Test for Induced Pluripotent Stem Cell (iPSC)-Derived Cell Products -- Concise Review: Methods and Cell Types Used to Generate Down Syndrome Induced Pluripotent Stem Cells -- Chapter 10: Immune Response -- The Possible Future Roles for iPSC-Derived Therapy for Autoimmune Diseases. |
Record Nr. | UNINA-9910598175403321 |
Basel : , : MDPI - Multidisciplinary Digital Publishing Institute, , 2015 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
IPS cells for modelling and treatment of human diseases / / edited by Michael J. Edel |
Pubbl/distr/stampa | Basel, Switzerland : , : MDPI - Multidisciplinary Digital Publishing Institute, , [2015] |
Descrizione fisica | 1 online resource (422 pages) : illustrations |
Disciplina | 572.8845 |
Soggetto topico | Genetic transcription |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto | List of Contributors -- About the Guest Editor -- Preface -- Chapter 1: Neuronal -- Opportunities and Limitations of Modelling Alzheimer's Disease with Induced Pluripotent Stem Cells -- Induced Pluripotent Stem Cells Derived from Alzheimer's Disease Patients: The Promise, the Hope and the Path Ahead -- iPSC-based Models to Unravel Key Pathogenetic Processes underlying Motor Neuron Diseases Development -- Chapter 2: Cardiac -- Bioengineering and Stem Cell Technology in the Treatment of Congenital Heart Disease -- Scalable Electrophysiological Investigation of iPS Cell Derived Cardiomyocytes Obtained by a Lentiviral Purification Strategy -- Clinical Potentials of Cardiomyocytes Derived from Patient-Specific Induced Pluripotent Stem Cells -- Chapter 3: Eye -- iPS Cells for Modelling and Treatment of Retinal Diseases -- Patient-Specific iPSC-Derived RPE for Modeling of Retinal Diseases -- Potential role of Induced Pluripotent Stem Cells (IPSCs) for Cell-Based Therapy of the Ocular Surface -- Chapter 4: Spinal Cord Injury -- The Potential for iPS-Derived Stem Cells as a Therapeutic Strategy for Spinal Cord Injury: Opportunities and Challenges -- The State of Play with iPSCs and Spinal Cord Injury Models -- Chapter 5: Liver -- Potential and Challenges of Induced Pluripotent Stem Cells in Liver Diseases Treatment -- Chapter 6: Muscle -- Myogenic Precursors from iPS Cells for Skeletal Muscle Cell Replacement Therapy -- Chapter 7: Bone -- The Use of Patient-Specific Induced Pluripotent Stem Cells (iPSCs) to Identify Osteoclast Defects in Rare Genetic Bone Disorders -- Chapter 8: Germ Cells -- Human iPS Cell-derived Germ Cells: Current Status and Clinical Potential -- Chapter 9: Genetic Disorders -- Comparing ESC and iPSC-Based Models for Human Genetic Disorders -- Design of a Tumorigenicity Test for Induced Pluripotent Stem Cell (iPSC)-Derived Cell Products -- Concise Review: Methods and Cell Types Used to Generate Down Syndrome Induced Pluripotent Stem Cells -- Chapter 10: Immune Response -- The Possible Future Roles for iPSC-Derived Therapy for Autoimmune Diseases. |
Record Nr. | UNINA-9910688474503321 |
Basel, Switzerland : , : MDPI - Multidisciplinary Digital Publishing Institute, , [2015] | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
Key transitions in animal evolution / editors, Rob Desalle, Bernd Schierwater |
Pubbl/distr/stampa | Enfield, N. H. : Science Publishers |
Descrizione fisica | xii, 434 p. : ill. (some col.) ; 24 cm |
Disciplina | 591.38 |
Altri autori (Persone) |
DeSalle, Rob
Schierwater, Bernd |
Soggetto topico |
Phylogeny
Evolution (Biology) Genetic transcription |
ISBN | 9781578086955 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Record Nr. | UNISALENTO-991001221619707536 |
Enfield, N. H. : Science Publishers | ||
Materiale a stampa | ||
Lo trovi qui: Univ. del Salento | ||
|
The Palgrave Handbook of Chinese Language Studies [[electronic resource] /] / edited by Zhengdao Ye |
Edizione | [1st ed. 2022.] |
Pubbl/distr/stampa | Singapore : , : Springer Nature Singapore : , : Imprint : Palgrave Macmillan, , 2022 |
Descrizione fisica | 1 online resource (1032 pages) |
Disciplina | 495.1 |
Soggetto topico |
Applied linguistics
Communication Linguistics Genetic transcription Intercultural communication Applied Linguistics Media and Communication Gene Transcription Intercultural Communication Xinès Manlleus (Lingüística) Anglès Lingüística |
Soggetto genere / forma | Llibres electrònics |
ISBN |
9789811609244
9789811609237 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto | 1. Research on Language Contact -- 2. Typology Studies in China -- 3. Cognitive Linguistics in China -- 4. Pragmatic Studies in China -- 5. Forensic Linguistics on Chinese -- 6. Sinitic Kinship Semantics -- 7. The Study of Classical Chinese in Europe -- 8. Language situations in contemporary China -- 9. Cantonese Diaspora -- 10. Language Contact: Shanghai Wu -- 11. Sinitic Languages along the Belt and Road -- 12. Hokkien in Diaspora -- 13. ‘Migrating concepts’ in Chinese -- 14. Chinese-English Bilingual Dictionaries -- 15. Chinese and the Search for a Universal Language: from Enlightenment to Contemporary times -- 16. Machine Translation -- 17. Translating Chinese Law -- 18. Chinese children literature in English translation -- 19. Translating films on ethnic minorities into English -- 20. Subtitling in English as cross-cultural mediation -- 21. Subtitling in Chinese -- 22. Technology in Chinese Language Teaching -- 23. Cultural Performance Approach in Chinese language teaching -- 24. Cultural Key Words in Mandarin Chinese -- 25. Mandarin-Speaking Children in Britain -- 26. Chinese as a mother tongue in the context of Global Business English Communication -- 27. Chinese and Singaporean English -- 28. From Kowtow to Gaokao: New Chinese Terms in English language media. |
Record Nr. | UNINA-9910585971203321 |
Singapore : , : Springer Nature Singapore : , : Imprint : Palgrave Macmillan, , 2022 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
Plant transcription factors : evolutionary, structural and functional aspects / / edited by Daniel H. Gonzalez |
Pubbl/distr/stampa | Amsterdam, Netherlands : , : Elsevier, , 2016 |
Descrizione fisica | 1 online resource (435 p.) |
Disciplina | 572.8652 |
Soggetto topico |
Plant genetic regulation
Plant cell differentiation Genetic transcription |
ISBN |
0-12-801127-0
0-12-800854-7 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Cover; Title page; Copyright Page; Contents; List of Contributors; Preface; Section A - General Aspects of Plant Transcription Factors; Chapter 1 - Introduction to Transcription Factor Structure and Function; 1.1 - Introduction: Transcription in eukaryotes; 1.2 - Structure of transcription factors; 1.3 - DNA recognition by transcription factors; 1.4 - DNA-binding domains; 1.5 - Protein-protein interactions; 1.6 - Regulation of transcription factor action; 1.7 - Plant transcription factors; Acknowledgments; References; Chapter 2 - Methods to Study Transcription Factor Structure and Function
2.5.1 - Methods for Protein-Protein Complex Identification2.5.1.1 - Yeast Two-Hybrid Assay (Y2H); 2.5.1.2 - Tandem and One-step Tag-Based Affinity Purification; 2.5.2 - Methods for Verification of Protein-Protein Interactions; 2.5.2.1 - Coimmunoprecipitation; 2.5.2.2 - In Vivo Split Methods; 2.5.2.3 - Resonance Energy Transfer Methods; Acknowledgments; References; Chapter 3 - General Aspects of Plant Transcription Factor Families; 3.1 - Introduction; 3.2 - Overview of the transcription cycle in eukaryotes; 3.2.1 - Core Promoter Elements and General Transcription Factors 3.6 - Plant-specific TF families |
Record Nr. | UNINA-9910797500603321 |
Amsterdam, Netherlands : , : Elsevier, , 2016 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
Plant transcription factors : evolutionary, structural and functional aspects / / edited by Daniel H. Gonzalez |
Pubbl/distr/stampa | Amsterdam, Netherlands : , : Elsevier, , 2016 |
Descrizione fisica | 1 online resource (435 p.) |
Disciplina | 572.8652 |
Soggetto topico |
Plant genetic regulation
Plant cell differentiation Genetic transcription |
ISBN |
0-12-801127-0
0-12-800854-7 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Cover; Title page; Copyright Page; Contents; List of Contributors; Preface; Section A - General Aspects of Plant Transcription Factors; Chapter 1 - Introduction to Transcription Factor Structure and Function; 1.1 - Introduction: Transcription in eukaryotes; 1.2 - Structure of transcription factors; 1.3 - DNA recognition by transcription factors; 1.4 - DNA-binding domains; 1.5 - Protein-protein interactions; 1.6 - Regulation of transcription factor action; 1.7 - Plant transcription factors; Acknowledgments; References; Chapter 2 - Methods to Study Transcription Factor Structure and Function
2.5.1 - Methods for Protein-Protein Complex Identification2.5.1.1 - Yeast Two-Hybrid Assay (Y2H); 2.5.1.2 - Tandem and One-step Tag-Based Affinity Purification; 2.5.2 - Methods for Verification of Protein-Protein Interactions; 2.5.2.1 - Coimmunoprecipitation; 2.5.2.2 - In Vivo Split Methods; 2.5.2.3 - Resonance Energy Transfer Methods; Acknowledgments; References; Chapter 3 - General Aspects of Plant Transcription Factor Families; 3.1 - Introduction; 3.2 - Overview of the transcription cycle in eukaryotes; 3.2.1 - Core Promoter Elements and General Transcription Factors 3.6 - Plant-specific TF families |
Record Nr. | UNINA-9910811445403321 |
Amsterdam, Netherlands : , : Elsevier, , 2016 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
RNA and DNA editing [[electronic resource] ] : molecular mechanisms and their integration into biological systems / / edited by Harold C. Smith |
Pubbl/distr/stampa | Hoboken, N.J., : Wiley-Interscience, c2008 |
Descrizione fisica | 1 online resource (464 p.) |
Disciplina | 572.8/8 |
Altri autori (Persone) | SmithHarold C |
Soggetto topico |
RNA editing
DNA Nucleotide sequence Genetic transcription |
ISBN |
1-281-23753-1
9786611237530 0-470-26226-5 0-470-26225-7 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
RNA AND DNA EDITING; CONTENTS; PREFACE; ACKNOWLEDGMENTS; CONTRIBUTORS; PART I DIVERSIFICATION OF THE PROTEOME THROUGH RNA AND DNA EDITING; CHAPTER 1 DIVERSIFYING EXON CODE THROUGH A-TO-I RNA EDITING; 1.1 Introduction and Background; 1.1.1 Initial Discovery and Context of A-to-I RNA Editing and ADARs; 1.1.2 Important Cases of Recoding by A-to-I Modification in Pre-mRNA; 1.1.3 Cis-Acting Features for A-to-I Editing; 1.1.4 Properties of the A-to-I Editing Machinery; 1.2 Main Questions in the Field and Approaches; 1.2.1 Biochemical Versus Computational Approaches; 1.2.2 Editing of miRNA Sequences
1.3 Future Directions: Evolution of Editing Sites and MachineryReferences; CHAPTER 2 ANTIBODY GENE DIVERSIFICATION BY AID-CATALYZED DNA EDITING; 2.1 Introduction; 2.2 Before AID; 2.2.1 Without DNA (Darkness) and with DNA (Light); 2.2.2 Prominent Early Models for Antibody Diversification; 2.2.3 How Protein Sequencing Technology Enabled an Understanding of Antibody Diversity; 2.2.4 Somatic DNA Rearrangements Underpin V(D)J Joining and Create the Primary Antibody Repertoire; 2.2.5 Additional Antibody Diversity by Somatic Hypermutation (and Gene Conversion in Some Animals) 2.2.6 Altering Antibody Function by Class Switch Recombination (Isotype Switching)2.3 After AID; 2.3.1 A Novel Deaminase Is Required for CSR, SHM, and IGC; 2.3.2 AID Is a DNA Cytosine Deaminase that Directly Triggers Antibody Diversification; 2.3.3 The Importance of Uracil Bases in DNA In Vivo; 2.3.4 Processing of AID-induced Lesions: The Molecular Mechanism of Somatic-Hypermutation; 2.3.5 Processing of AID-induced Lesions: The Molecular Mechanism of Immunoglobulin Gene Conversion; 2.3.6 Processing of AID-induced Lesions: The Molecular Mechanism of Class Switch Recombination 2.4 Hot Areas and Speculations2.4.1 Immunodeficiency Syndromes Caused by Defects in AID-Mediated Ig Gene Diversification; 2.4.2 Regulating the DNA Mutator Activity of AID; 2.4.3 Misregulation of AID and Cancer; 2.4.4 AID Is But One Member of a Much Larger Family of Polynucleotide Deaminases; 2.5 Conclusions; Acknowledgments; References; CHAPTER 3 PROTEIN-PROTEIN AND RNA-PROTEIN INTERACTIONS IN U-INSERTION/DELETION RNA EDITING COMPLEXES; 3.1 A Bizarre Phenomenon and its Raison D'être; 3.2 The Catalytic Mechanism and Machinery 3.3 Extent of U-Insertion/Deletion RNA Editing in Trypanosoma and Leishmania Species3.4 Functional Studies of Editing Complex Subunits; 3.4.1 REN1, REN2, and MP67. Endonuclease Homologs; 3.4.2 REX1 and REX2. Exonuclease Homologs; 3.4.3 RET2. TUTase; 3.4.4 REL1 and REL2. Ligase Homologs; 3.4.5 MP81, MP63, MP42, MP46, MP44, MP24, MP18. Structural Components; 3.5 RNA-Protein Interactions: Isolated Subunits and Assembled Editing Complexes; 3.5.1 MP42; 3.5.2 MP24; 3.5.3 RNA-Protein Interactions in Assembled Editing Complexes; 3.6 Concluding Remarks; Acknowledgments; References CHAPTER 4 MACHINERY OF RNA EDITING IN PLANT ORGANELLES |
Record Nr. | UNINA-9910145699603321 |
Hoboken, N.J., : Wiley-Interscience, c2008 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
RNA and DNA editing [[electronic resource] ] : molecular mechanisms and their integration into biological systems / / edited by Harold C. Smith |
Pubbl/distr/stampa | Hoboken, N.J., : Wiley-Interscience, c2008 |
Descrizione fisica | 1 online resource (464 p.) |
Disciplina | 572.8/8 |
Altri autori (Persone) | SmithHarold C |
Soggetto topico |
RNA editing
DNA Nucleotide sequence Genetic transcription |
ISBN |
1-281-23753-1
9786611237530 0-470-26226-5 0-470-26225-7 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
RNA AND DNA EDITING; CONTENTS; PREFACE; ACKNOWLEDGMENTS; CONTRIBUTORS; PART I DIVERSIFICATION OF THE PROTEOME THROUGH RNA AND DNA EDITING; CHAPTER 1 DIVERSIFYING EXON CODE THROUGH A-TO-I RNA EDITING; 1.1 Introduction and Background; 1.1.1 Initial Discovery and Context of A-to-I RNA Editing and ADARs; 1.1.2 Important Cases of Recoding by A-to-I Modification in Pre-mRNA; 1.1.3 Cis-Acting Features for A-to-I Editing; 1.1.4 Properties of the A-to-I Editing Machinery; 1.2 Main Questions in the Field and Approaches; 1.2.1 Biochemical Versus Computational Approaches; 1.2.2 Editing of miRNA Sequences
1.3 Future Directions: Evolution of Editing Sites and MachineryReferences; CHAPTER 2 ANTIBODY GENE DIVERSIFICATION BY AID-CATALYZED DNA EDITING; 2.1 Introduction; 2.2 Before AID; 2.2.1 Without DNA (Darkness) and with DNA (Light); 2.2.2 Prominent Early Models for Antibody Diversification; 2.2.3 How Protein Sequencing Technology Enabled an Understanding of Antibody Diversity; 2.2.4 Somatic DNA Rearrangements Underpin V(D)J Joining and Create the Primary Antibody Repertoire; 2.2.5 Additional Antibody Diversity by Somatic Hypermutation (and Gene Conversion in Some Animals) 2.2.6 Altering Antibody Function by Class Switch Recombination (Isotype Switching)2.3 After AID; 2.3.1 A Novel Deaminase Is Required for CSR, SHM, and IGC; 2.3.2 AID Is a DNA Cytosine Deaminase that Directly Triggers Antibody Diversification; 2.3.3 The Importance of Uracil Bases in DNA In Vivo; 2.3.4 Processing of AID-induced Lesions: The Molecular Mechanism of Somatic-Hypermutation; 2.3.5 Processing of AID-induced Lesions: The Molecular Mechanism of Immunoglobulin Gene Conversion; 2.3.6 Processing of AID-induced Lesions: The Molecular Mechanism of Class Switch Recombination 2.4 Hot Areas and Speculations2.4.1 Immunodeficiency Syndromes Caused by Defects in AID-Mediated Ig Gene Diversification; 2.4.2 Regulating the DNA Mutator Activity of AID; 2.4.3 Misregulation of AID and Cancer; 2.4.4 AID Is But One Member of a Much Larger Family of Polynucleotide Deaminases; 2.5 Conclusions; Acknowledgments; References; CHAPTER 3 PROTEIN-PROTEIN AND RNA-PROTEIN INTERACTIONS IN U-INSERTION/DELETION RNA EDITING COMPLEXES; 3.1 A Bizarre Phenomenon and its Raison D'être; 3.2 The Catalytic Mechanism and Machinery 3.3 Extent of U-Insertion/Deletion RNA Editing in Trypanosoma and Leishmania Species3.4 Functional Studies of Editing Complex Subunits; 3.4.1 REN1, REN2, and MP67. Endonuclease Homologs; 3.4.2 REX1 and REX2. Exonuclease Homologs; 3.4.3 RET2. TUTase; 3.4.4 REL1 and REL2. Ligase Homologs; 3.4.5 MP81, MP63, MP42, MP46, MP44, MP24, MP18. Structural Components; 3.5 RNA-Protein Interactions: Isolated Subunits and Assembled Editing Complexes; 3.5.1 MP42; 3.5.2 MP24; 3.5.3 RNA-Protein Interactions in Assembled Editing Complexes; 3.6 Concluding Remarks; Acknowledgments; References CHAPTER 4 MACHINERY OF RNA EDITING IN PLANT ORGANELLES |
Record Nr. | UNINA-9910807054603321 |
Hoboken, N.J., : Wiley-Interscience, c2008 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
RNA interference in practice [[electronic resource] /] / Ute Schepers |
Autore | Schepers Ute |
Pubbl/distr/stampa | Weinheim, : Wiley-VCH, 2005 |
Descrizione fisica | 1 online resource (338 p.) |
Disciplina | 572.8845 |
Soggetto topico |
RNA editing
Genetic transcription |
Soggetto genere / forma | Electronic books. |
ISBN |
1-280-51971-1
9786610519712 3-527-60439-1 3-527-60437-5 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
RNA Interference in Practice; Preface; Contents; 1 Introduction: RNA interference, the ""Breakthrough of the Year 2002""; 1.1 RNAi as a Tool for Functional Genomics; 1.2 Mechanism of RNAi; 1.3 Dicer - the Initiator to "Dice" the dsRNA?; 1.4 miRNAs versus siRNAs: Two Classes of Small RNAs Using the RNAi Pathway?; 1.5 RISC - the Effector to "Slice" the mRNA?; 1.6 Are RNA-Dependent RNA Polymerases (RdRps) Responsible for the Catalytic Nature of RNAi?; 1.7 Is RNAi Involved in the Regulation of Gene Expression?; 1.8 RNAi in Mammals; 1.9 Practical Approaches; 1.10 References
2 RNAi in Caenorhabditis elegans2.1 Introduction; 2.2 Application of RNAi in C. elegans; 2.3 Target Sequence Evaluation; 2.4 dsRNA Synthesis; 2.4.2 Generation of the DNA Template; 2.4.2.1 Plasmid Templates; 2.4.2.2 DNA Templates Derived by PCR/RT-PCR; 2.4.2 In-vitro dsRNA Transcription; 2.5 Delivery of dsRNA; 2.5.1 General Information on the C. elegans Anatomy; 2.5.2 C. elegans Strains for Silencing; 2.5.3 Culturing the Worms; 2.5.4 Microinjection Protocol; 2.5.5 Soaking Protocol; 2.5.6 RNAi Feeding Protocol; 2.5.7 DNA Templates for dsRNA Expression in Feeding E. coli 2.5.8 DNA Templates for Hairpin RNA Expression2.5.8.1 C. elegans Promoters; 2.5.8.2 Inverted Repeat Constructs; 2.6 Mounting Animals for Microscopy; 2.7 Genome Wide Screens; 2.7.1 C. elegans RNAi Library; 2.8 Selected Literature on C. elegans Research; 2.9 Useful C. elegans webpages; 2.10 References; 3 RNAi in Drosophila; 3.1 Introduction; 3.2 Application of RNAi in Drosophila; 3.2.1 dsRNA from Linear DNA Templates; 3.2.2 dsRNA from Inverted Repeat DNA; 3.2.3 Inducible Expression in Drosophila Cell Lines; 3.2.4 Limitations; 3.3 dsRNA Synthesis; 3.3.1 In-vitro dsRNA Transcription 3.3.2 Inverted Repeat DNA3.4 Injections; 3.4.1 Injection Services; 3.4.2 Injection Method; 3.4.3 DsRNA or Inverted Repeat DNA Preparation; 3.4.4 Embryo Collection; 3.5 Cell Lines; 3.6 Protocols; 3.6.1 Thawing and Maintenance of S2 Cells; 3.6.2 Freezing Protocol; 3.7 RNAi in S2 Cells; 3.7.1 dsRNA Transfection Using the Calcium Phosphate Method; 3.7.2 dsRNA Soaking of S2 Cells; 3.8 High-Throughput Screens; 3.8.1 Drosophila RNAi Library; 3.9 Useful Webpages for Drosophila Research; 3.10 Books and Literature on Drosophila; 3.11 References; 4 RNAi in Mammals; 4.1 Introduction 4.2 Transient RNAi in Cell Culture4.2.1 Chemical Synthesis and Modifications of siRNAs; 4.2.1.1 Advantages; 4.2.1.2 Limitations; 4.2.2 Custom Synthesis of siRNA Oligos; 4.2.3 siRNA Design Rules; 4.2.3.1 siRNA Strand Bias and Off-Gene Targeting; 4.2.3.2 Improvements in siRNA Stability; 4.2.3.3 siRNA Design: Novel Modifications of the "Tuschl Rules"; 4.2.3.4 Homology Search by BLAST, FASTA, or Smith-Waterman Algorithm; 4.2.3.5 Troubleshooting; 4.2.3.6 siRNA Design Programs and Algorithms; 4.2.3.7 Preparation of siRNA Duplexes; 4.2.4 Enzymatic Synthesis of siRNAs 4.2.4.1 Designing DNA Oligonucleotides |
Record Nr. | UNINA-9910144557603321 |
Schepers Ute | ||
Weinheim, : Wiley-VCH, 2005 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|