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Dissecting Regulatory Interactions of RNA and Protein [[electronic resource] ] : Combining Computation and High-throughput Experiments in Systems Biology / / by Marvin Jens
Dissecting Regulatory Interactions of RNA and Protein [[electronic resource] ] : Combining Computation and High-throughput Experiments in Systems Biology / / by Marvin Jens
Autore Jens Marvin
Edizione [1st ed. 2014.]
Pubbl/distr/stampa Cham : , : Springer International Publishing : , : Imprint : Springer, , 2014
Descrizione fisica 1 online resource (115 p.)
Disciplina 574.873282
Collana Springer Theses, Recognizing Outstanding Ph.D. Research
Soggetto topico Computational intelligence
Systems biology
Biological systems
Gene expression
Biochemistry
Computational Intelligence
Systems Biology
Gene Expression
Biochemistry, general
ISBN 3-319-07082-7
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Introduction -- Computational analysis of PAR-CLIP data -- Transcriptome-wide analysis of regulatory interactions of the RNA-binding protein HuR -- Binding site occupancy with competition interactions in equilibrium -- Circular RNAs are a large class of animal RNAs with regulatory potency -- Discussion -- Methods.
Record Nr. UNINA-9910299748203321
Jens Marvin  
Cham : , : Springer International Publishing : , : Imprint : Springer, , 2014
Materiale a stampa
Lo trovi qui: Univ. Federico II
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DNA Cloning: A Hands-on Approach [[electronic resource] /] / by Seok-Yong Choi, Hyunju Ro, Hankuil Yi
DNA Cloning: A Hands-on Approach [[electronic resource] /] / by Seok-Yong Choi, Hyunju Ro, Hankuil Yi
Autore Choi Seok-Yong
Edizione [1st ed. 2019.]
Pubbl/distr/stampa Dordrecht : , : Springer Netherlands : , : Imprint : Springer, , 2019
Descrizione fisica 1 online resource (139 pages)
Disciplina 574.873282
Soggetto topico Gene expression
Genetic engineering
Plant genetics
Animal genetics
Gene Expression
Genetic Engineering
Plant Genetics and Genomics
Animal Genetics and Genomics
ISBN 94-024-1662-5
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Part I. What is cloning? -- Chapter 1.1 Definition of cloning -- Chapter 1.2 Discovering a new gene -- Chapter 1.3 Cloning in the past -- Chapter 1.4 Cloning in the present -- Part II. A prerequisite for cloning -- Chapter 2.1 Software useful for cloning design -- Chapter 2.2 Vector, plasmid, construct and the Kozak consensus sequence -- Chapter 2.3 Multiple cloning sites (MCS) -- Chapter 2.4 Restriction endonucleases and star activity -- Chapter 2.5 Agarose gel electrophoresis -- Chapter 2.6 Pouring LB agar plates -- Chapter 2.7 Competent cells -- Chapter 2.8 The conversion of DNA mass into molar concentration -- Chapter 2.9 Upon receiving new plasmids -- Chapter 2.10 cDNA library -- Part III. The first step in cloning -- Chapter 3.1 Cut & paste -- Chapter 3.2 DNA sequencing and direct sequencing -- Chapter 3.3 PCR and nested PCR -- Chapter 3.4 Fill-in (Full & Partial) -- Chapter 3.5 Compatible cohesive ends -- Chapter 3.6 Methylation -- Chapter 3.7 Three-piece ligation -- Chapter 3.8 Site-directed mutagenesis -- Chapter 3.9 Structure of plant transformation vectors -- Chapter 3.10 Transformation of R. radiobacter -- Part IV. The next step of cloning -- Chapter 4.1 How to insert a DNA fragment into a gene -- Chapter 4.2 How to delete an internal region of a gene -- Chapter 4.3 How to insert an epitope tag into a gene -- Chapter 4.4 Translational fusion vs. transcriptional fusion -- Part V. The last steps of cloning -- Chapter 5.1 Method for cloning similar genes in different species -- Chapter 5.2 RACE (rapid amplification of cDNA ends) -- Chapter 5.3 BAC recombineering -- Chapter 5.4 Old trick: partial digestion -- Chapter 5.5 Modification of a vector -- Chapter 5.6 When you notice a frame shift mutation upon cloning -- Chapter 5.7 Reality of cloning: an extremely unlucky case -- Part VI. Methods that make your cloning life easier -- Chapter 6.1 TA cloning and production of a T-vector -- Chapter 6.2 TOPO TA cloning -- Chapter 6.3 Gateway cloning -- Chapter 6.4 Golden Gate Assembly for a modular cloning -- Chapter 6.5 In-Fusion Sequence and Ligation-Independent Cloning (In-Fusion SLIC) -- Chapter 6.6 T4 DNA Polymerase Sequence-and Ligation-Independent Cloning (T4 DNA Pol SLIC) -- Chapter 6.7 Non-template PCR cloning -- Part VII. Advice to cloners -- Chapter 7.1 When cloning is not going well -- Chapter 7.2 Keep your cloning data organized -- Appendix 1. Further readings -- Appendix 2. Abbreviations -- Appendix 3. Index.
Record Nr. UNINA-9910337954003321
Choi Seok-Yong  
Dordrecht : , : Springer Netherlands : , : Imprint : Springer, , 2019
Materiale a stampa
Lo trovi qui: Univ. Federico II
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DNA Methyltransferases - Role and Function [[electronic resource] /] / edited by Albert Jeltsch, Renata Z. Jurkowska
DNA Methyltransferases - Role and Function [[electronic resource] /] / edited by Albert Jeltsch, Renata Z. Jurkowska
Edizione [1st ed. 2016.]
Pubbl/distr/stampa Cham : , : Springer International Publishing : , : Imprint : Springer, , 2016
Descrizione fisica 1 online resource (VIII, 535 p. 61 illus., 57 illus. in color.)
Disciplina 572.86
Collana Advances in Experimental Medicine and Biology
Soggetto topico Cell biology
Gene expression
Cytogenetics
Cell Biology
Gene Expression
ISBN 3-319-43624-4
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Mechanisms and biological roles of DNA methylation and DNA methyltransferases – from past achievements to future challenges -- DNA and RNA pyrimidine nucleobase alkylation at the carbon-5 position -- Bacterial DNA methylation and methylomes -- Domain structure of the Dnmt1, Dnmt3a, and Dnmt3b DNA methyltransferases -- Enzymology of mammalian DNA methyltransferases -- Genetic studies on mammalian DNA methyltransferases -- The role of DNA methylation in cancer -- Structure and mechanism of plant DNA methyltransferases -- DNA methylation and gene regulation in honey bees: from genome-wide analyses to obligatory epi-alleles -- N6-methyladenine: a conserved and dynamic DNA mark -- Pathways of DNA demethylation -- Structure and function of TET enzymes -- Proteins that read DNA methylation -- DNA base flipping – a general mechanism for writing, reading, and erasing DNA modifications -- Current and emerging technologies for the analysis of the genome-wide and locus-specific DNA methylation patterns -- DNA methyltransferase inhibitors – development and applications -- Rewriting DNA methylation signatures at will: the curable genome within reach? -- Engineering and directed evolution of DNA methyltransferases -- DNA labeling using DNA methyltransferases.
Record Nr. UNINA-9910150454903321
Cham : , : Springer International Publishing : , : Imprint : Springer, , 2016
Materiale a stampa
Lo trovi qui: Univ. Federico II
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DNA microarrays and gene expression / / Pierre Baldi and G. Wesley Hatfield [[electronic resource]]
DNA microarrays and gene expression / / Pierre Baldi and G. Wesley Hatfield [[electronic resource]]
Autore Baldi Pierre
Pubbl/distr/stampa Cambridge : , : Cambridge University Press, , 2002
Descrizione fisica 1 online resource (xvi, 213 pages) : digital, PDF file(s)
Disciplina 572.8/65
Soggetto topico DNA microarrays
Gene expression
ISBN 1-107-13017-4
1-280-16041-1
9786610160419
0-511-06321-0
0-511-11922-4
1-139-14694-7
0-511-05688-5
0-511-32267-4
0-511-54177-5
0-511-07167-1
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Cover; Half-title; Title; Copyright; Contents; Preface; 1 A brief history of genomics; 2 DNA array formats; 3 DNA array readout methods; 4 Gene expression profiling experiments: Problems, pitfalls, and solutions; 5 Statistical analysis of array data: Inferring changes; 6 Statistical analysis of array data: Dimensionality reduction,clustering,and regulatory regions; 7 The design, analysis,and interpretation of gene expression profiling experiments; 8 Systems biology; Appendix A; Appendix B; Appendix C; Appendix D; Index
Altri titoli varianti DNA Microarrays & Gene Expression
Record Nr. UNINA-9910450497003321
Baldi Pierre  
Cambridge : , : Cambridge University Press, , 2002
Materiale a stampa
Lo trovi qui: Univ. Federico II
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DNA microarrays and gene expression / / Pierre Baldi and G. Wesley Hatfield [[electronic resource]]
DNA microarrays and gene expression / / Pierre Baldi and G. Wesley Hatfield [[electronic resource]]
Autore Baldi Pierre
Pubbl/distr/stampa Cambridge : , : Cambridge University Press, , 2002
Descrizione fisica 1 online resource (xvi, 213 pages) : digital, PDF file(s)
Disciplina 572.8/65
Soggetto topico DNA microarrays
Gene expression
ISBN 1-107-13017-4
1-280-16041-1
9786610160419
0-511-06321-0
0-511-11922-4
1-139-14694-7
0-511-05688-5
0-511-32267-4
0-511-54177-5
0-511-07167-1
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Cover; Half-title; Title; Copyright; Contents; Preface; 1 A brief history of genomics; 2 DNA array formats; 3 DNA array readout methods; 4 Gene expression profiling experiments: Problems, pitfalls, and solutions; 5 Statistical analysis of array data: Inferring changes; 6 Statistical analysis of array data: Dimensionality reduction,clustering,and regulatory regions; 7 The design, analysis,and interpretation of gene expression profiling experiments; 8 Systems biology; Appendix A; Appendix B; Appendix C; Appendix D; Index
Altri titoli varianti DNA Microarrays & Gene Expression
Record Nr. UNINA-9910783118803321
Baldi Pierre  
Cambridge : , : Cambridge University Press, , 2002
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
DNA microarrays and gene expression / / Pierre Baldi and G. Wesley Hatfield [[electronic resource]]
DNA microarrays and gene expression / / Pierre Baldi and G. Wesley Hatfield [[electronic resource]]
Autore Baldi Pierre
Pubbl/distr/stampa Cambridge : , : Cambridge University Press, , 2002
Descrizione fisica 1 online resource (xvi, 213 pages) : digital, PDF file(s)
Disciplina 572.8/65
Soggetto topico DNA microarrays
Gene expression
ISBN 1-107-13017-4
1-280-16041-1
9786610160419
0-511-06321-0
0-511-11922-4
1-139-14694-7
0-511-05688-5
0-511-32267-4
0-511-54177-5
0-511-07167-1
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Cover; Half-title; Title; Copyright; Contents; Preface; 1 A brief history of genomics; 2 DNA array formats; 3 DNA array readout methods; 4 Gene expression profiling experiments: Problems, pitfalls, and solutions; 5 Statistical analysis of array data: Inferring changes; 6 Statistical analysis of array data: Dimensionality reduction,clustering,and regulatory regions; 7 The design, analysis,and interpretation of gene expression profiling experiments; 8 Systems biology; Appendix A; Appendix B; Appendix C; Appendix D; Index
Altri titoli varianti DNA Microarrays & Gene Expression
Record Nr. UNINA-9910829175403321
Baldi Pierre  
Cambridge : , : Cambridge University Press, , 2002
Materiale a stampa
Lo trovi qui: Univ. Federico II
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DNA modifications in the brain : neuroepigenetic regulation of gene expression / / Timothy Bredy
DNA modifications in the brain : neuroepigenetic regulation of gene expression / / Timothy Bredy
Autore Bredy Timothy
Pubbl/distr/stampa Amsterdam, [Netherlands] : , : Academic Press, , 2017
Descrizione fisica 1 online resource (182 pages) : illustrations, tables
Disciplina 574.87322
Soggetto topico Gene expression
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Preface -- History and modern view on DNA modifications in the brain / B.F. Vanyushin and V.V. Ashapkin -- Approaches to detecting DNA base modification in the brain / X. Li and W. Wei -- Active DNA demethylation in neurodevelopment / Y. Kang, Z. Wang and P. Jin -- TET and 5hmC in neurodevelopment and the adult brain / M. Fasolino, S.A. Welsh and Z. Zhou -- Beyond mCG : DNA methylation in noncanonical sequence context / E.A. Mukamel and R. Lister -- DNA modifications and memory / J.J. Day -- Measuring CpG methylation by SMRT sequencing / Y. Suzuki, J. Korlach and S. Morishita -- Epigenetic modifications of DNA and drug addiction / J. Feng and E.J. Nestler -- What does the future hold for the study of nucleic acid modifications in the brain? / P.R. Marshall and T.W. Bredy.
Record Nr. UNINA-9910583314503321
Bredy Timothy  
Amsterdam, [Netherlands] : , : Academic Press, , 2017
Materiale a stampa
Lo trovi qui: Univ. Federico II
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Early Life Origins of Ageing and Longevity [[electronic resource] /] / edited by Alexander Vaiserman
Early Life Origins of Ageing and Longevity [[electronic resource] /] / edited by Alexander Vaiserman
Edizione [1st ed. 2019.]
Pubbl/distr/stampa Cham : , : Springer International Publishing : , : Imprint : Springer, , 2019
Descrizione fisica 1 online resource (313 pages) : illustrations
Disciplina 612.67
Collana Healthy Ageing and Longevity
Soggetto topico Aging
Developmental biology
Gene expression
Geriatrics
Biogerontology
Developmental Biology
Gene Expression
Geriatrics/Gerontology
ISBN 3-030-24958-1
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto SECTION I OVERVIEW -- Introductory chapter -- SECTION II EXPERIMENTAL STUDY OF DOHAD -- Early Life Programming of Aging in Genetically Long-Lived Mice -- Immunological Basis of In Utero Programming of Adult Disease -- Developmental Programming of the GH/IGF Axis and Long Term Health -- Programming of Stress Responses -- Early-life Nutritional Programming of Adult Health Status -- Effects of In-Utero Conditions on Adult Feeding Preferences -- IUGR-Related Programming of Metabolic Syndrome and Type 2 Diabetes -- Early-life Programming of Osteoporosis -- Developmental Epigenetic Programming of Skeletal Muscle Aging -- Effect of fetal and child health on kidney development and long-term risk of hypertension and kidney disease. -- Developmental Programming of Neuroimmune Function -- Epigenetics and neurodegeneration: role of early-life nutrition -- SECTION III EPIDEMIOLOGICAL EVIDENCE -- Prenatal Undernutrition and Ageing and Longevity -- Influence of Maternal Obesity on the Long-Term Health of Offspring -- Early Life Predictors of Women's Health and Ageing -- Early-Life Predictors of Exceptional Longevity -- Prenatal Undernutrition and Effects on Physical and Mental Health -- Ancestral and early origins of adult mortality - a multigenerational approach -- SECTION IV PERSPECTIVES AND IMPLICATIONS -- Social Policy and Public Health Perspectives of DOHaD: Implications for Policy Advocacy, Cross-Sectorial Collaboration and Health Promotion -- Index.
Record Nr. UNINA-9910349451603321
Cham : , : Springer International Publishing : , : Imprint : Springer, , 2019
Materiale a stampa
Lo trovi qui: Univ. Federico II
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Endometrial gene expression : an emerging paradigm for reproductive disorders / / Editor, Joanne Kwak-Kim
Endometrial gene expression : an emerging paradigm for reproductive disorders / / Editor, Joanne Kwak-Kim
Edizione [1st ed. 2020.]
Pubbl/distr/stampa Cham, Switzerland : , : Springer, , [2020]
Descrizione fisica 1 online resource (XIII, 220 p. 15 illus. in color.)
Disciplina 618.1
Soggetto topico Gynecology
Gene expression
Reproductive health
ISBN 3-030-28584-7
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Endometrial Development and Its Fine Structure -- Molecular Biology Techniques for Endometrial Gene Expression: Recent Technological Advances -- T Cell-related Endometrial Gene Expression Abnormalities -- Role of Immunoregulatory Cytokine IL-15 in the Endometrium -- Endometrial Gene Expression in Women with Repeated Implantation Failure -- Endometrial Receptivity by ERA for Infertility -- Endometrium Gene Expression and Epigenetic Regulation in Reproductive Failure -- Endometrial Gene Expression for HHV6 -- Infection and Endometrial Gene Expression: HHV6 and Infertility -- Endometrial Immune Inflammatory Gene Signature in Endometriosis -- Gene Expression in Endometriosis -- MicroRNA and Endometriosis -- Metabolic and Nutritional Impact on Endometrial Gene Expression and Reproductive Disorders.
Record Nr. UNINA-9910369931503321
Cham, Switzerland : , : Springer, , [2020]
Materiale a stampa
Lo trovi qui: Univ. Federico II
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Epigenesis, Genetic [[electronic resource] /] / edited by Elisabetta Baldi, Monica Muratori
Epigenesis, Genetic [[electronic resource] /] / edited by Elisabetta Baldi, Monica Muratori
Edizione [2nd ed. 2019.]
Pubbl/distr/stampa Cham : , : Springer International Publishing : , : Imprint : Springer, , 2019
Descrizione fisica 1 online resource (216 pages)
Disciplina 573.21
Collana Advances in Experimental Medicine and Biology
Soggetto topico Gene expression
Medical genetics
Human physiology
Gene Expression
Gene Function
Human Physiology
ISBN 3-030-21664-0
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Genetic factors affecting sperm chromatin structure -- Mechanisms of origin of chromosome deletions and rearrangements in spermatozoa -- The sperm epigenome: implications for ART outcomes -- Epigenetic transgenerational inheritance -- Environmental genetic and epigenetic sperm alterations -- Sperm DNA fragmentation: mechanisms of origin -- Sperm DNA fragmentation: consequences for reproduction -- Oxidative damage to sperm DNA: attack and defence -- Interventions to prevent sperm DNA damage effects on reproduction.-Cryopreservation of sperm: effects on chromatin and strategies to prevent them -- Effect on sperm DNA quality following sperm selection for ART: new insights -- Sperm DNA damage in cancer patients -- Index.
Record Nr. UNINA-9910349454403321
Cham : , : Springer International Publishing : , : Imprint : Springer, , 2019
Materiale a stampa
Lo trovi qui: Univ. Federico II
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