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Molecular biology and genetic engineering [[electronic resource] /] / P.K. Gupta
| Molecular biology and genetic engineering [[electronic resource] /] / P.K. Gupta |
| Autore | Gupta P. K |
| Pubbl/distr/stampa | Meerut, India, : Rastogi Publications, 2008 |
| Descrizione fisica | 1 online resource (613 p.) |
| Disciplina | 572.8 |
| Soggetto topico |
Molecular biology
Genetic engineering |
| ISBN |
1-64287-613-5
1-283-22699-5 9786613226990 1-4416-7214-1 600-00-4429-1 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | pt.1. Molecular biology -- pt. 2. Genetic engineering. |
| Record Nr. | UNINA-9910785136703321 |
Gupta P. K
|
||
| Meerut, India, : Rastogi Publications, 2008 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Molecular biology and genetic engineering [[electronic resource] /] / P.K. Gupta
| Molecular biology and genetic engineering [[electronic resource] /] / P.K. Gupta |
| Autore | Gupta P. K |
| Pubbl/distr/stampa | Meerut, India, : Rastogi Publications, 2008 |
| Descrizione fisica | 1 online resource (613 p.) |
| Disciplina | 572.8 |
| Soggetto topico |
Molecular biology
Genetic engineering |
| ISBN |
1-64287-613-5
1-283-22699-5 9786613226990 1-4416-7214-1 600-00-4429-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | pt.1. Molecular biology -- pt. 2. Genetic engineering. |
| Record Nr. | UNINA-9910822754703321 |
|
Gupta P. K
|
||
| Meerut, India, : Rastogi Publications, 2008 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Molecular biology and genomics [[electronic resource] /] / Cornel Mulhardt ; translated by E.W. Beese
| Molecular biology and genomics [[electronic resource] /] / Cornel Mulhardt ; translated by E.W. Beese |
| Autore | Mülhardt Cornel |
| Pubbl/distr/stampa | Burlington, MA, : Academic Press, c2007 |
| Descrizione fisica | 1 online resource (273 p.) |
| Disciplina | 572.8 |
| Collana | The experimenter series |
| Soggetto topico |
Molecular biology - Research - Methodology
Genomics - Research - Methodology |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-280-74685-8
9786610746859 0-08-046766-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Molecular Biology and Genomics; Copyright Page; Table of Contents; Foreword; Foreword to the First Edition; Acknowledgments; Chapter 1 What Is Molecular Biology?; 1.1 The Substrate of Molecular Biology: The Molecular World for Beginners; 1.2 What Is Required for This Work?; 1.3 Safety in the Laboratory; Chapter 2 Fundamental Methods; 2.1 Differences in Nucleic Acids; 2.2 Precipitation and Concentration of Nucleic Acids; 2.2.1 Alcohol Precipitation; 2.2.2 Concentrators; 2.2.3 Savant Speed Vac; 2.2.4 Salting Out; 2.3 Purification of Nucleic Acids; 2.3.1 Phenol-Chloroform Extraction
2.3.2 Precipitation Using Polyethylene Glycol2.3.3 Protein-Binding Filter Membranes; 2.3.4 Anion-Exchange Columns; 2.3.5 Glass Milk; 2.3.6 Cesium Chloride Density Gradient; 2.3.7 Dialysis; 2.4 Determining the Concentration of Nucleic Acid Solutions; 2.4.1 Optical Density Measurements with the Aid of Absorption Spectrometry; 2.4.2 Determination of Concentration by Means of Agarose Gels; 2.4.3 Dot Quantification; 2.4.4 Fluorometric Determination; 2.4.5 Nucleic Acid Dipsticks; 2.4.6 Enzymatic Evidence; 2.5 Methods of DNA Preparation; 2.5.1 Preparation of Plasmid DNA on a Small Scale 2.5.2 Preparation of Plasmid DNA on a Large Scale2.5.3 Bacterial Media; 2.5.4 Preparation of Phage DNA; 2.5.5 Preparation of Single-Stranded DNA with the Aid of Helper Phages; 2.5.6 Preparation of Genomic DNA; Chapter 3 The Tools; 3.1 Restriction Enzymes; 3.1.1 Nomenclature; 3.1.2 The Activity Test; 3.1.3 Making a Restriction Digestion; 3.1.4 Difficulties Associated with Restriction Digestion; 3.1.5 Works of Reference for Restriction Digestion; 3.2 Gels; 3.2.1 Agarose Gels; 3.2.2 Isolating DNA Fragments from Agarose Gels; 3.2.3 Polyacrylamide Gels 3.2.4 Isolating DNA Fragments from Polyacrylamide Gels3.2.5 Pulse-Field Gel Electrophoresis; 3.2.6 Capillary Electrophoresis; 3.3 Blotting; 3.3.1 Southern Blots; 3.3.2 Northern Blots; 3.3.3 Dot Blots and Slot Blots; Chapter 4 The Polymerase Chain Reaction; 4.1 Standard Polymerase Chain Reaction; 4.2 Suggestions for Improving the Polymerase Chain Reaction; 4.2.1 Nested Polymerase Chain Reaction; 4.2.2 Multiplex Polymerase Chain Reaction; 4.2.3 Amplification of Longer DNA Fragments; 4.3 PCR Applications; 4.3.1 Reverse Transcription-Polymerase Chain Reaction 4.3.2 Rapid Amplification of cDNA Ends4.3.3 Amplification of Coincidental Products; 4.3.4 Classic Quantitative Polymerase Chain Reaction; 4.3.5 Real-Time Quantitative Polymerase Chain Reaction; 4.3.6 Inverse Polymerase Chain Reaction; 4.3.7 Biotin-RAGE Method and Supported Polymerase Chain Reaction; 4.3.8 Mutagenesis with Modified Primers; 4.3.9 Amplification Refractory Mutation System; 4.3.10 In Situ Polymerase Chain Reaction; 4.3.11 Cycle Sequencing; 4.3.12 cDNA Synthesis; 4.3.13 Single-Cell Polymerase Chain Reaction; Chapter 5 RNA; 5.1 Inactivating RNases; 5.2 Methods of RNA Isolation 5.2.1 Single-Step Method |
| Record Nr. | UNINA-9910458487003321 |
|
Mülhardt Cornel
|
||
| Burlington, MA, : Academic Press, c2007 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Molecular biology and genomics [[electronic resource] /] / Cornel Mulhardt ; translated by E.W. Beese
| Molecular biology and genomics [[electronic resource] /] / Cornel Mulhardt ; translated by E.W. Beese |
| Autore | Mülhardt Cornel |
| Pubbl/distr/stampa | Burlington, MA, : Academic Press, c2007 |
| Descrizione fisica | 1 online resource (273 p.) |
| Disciplina | 572.8 |
| Collana | The experimenter series |
| Soggetto topico |
Molecular biology - Research - Methodology
Genomics - Research - Methodology |
| ISBN |
1-280-74685-8
9786610746859 0-08-046766-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Molecular Biology and Genomics; Copyright Page; Table of Contents; Foreword; Foreword to the First Edition; Acknowledgments; Chapter 1 What Is Molecular Biology?; 1.1 The Substrate of Molecular Biology: The Molecular World for Beginners; 1.2 What Is Required for This Work?; 1.3 Safety in the Laboratory; Chapter 2 Fundamental Methods; 2.1 Differences in Nucleic Acids; 2.2 Precipitation and Concentration of Nucleic Acids; 2.2.1 Alcohol Precipitation; 2.2.2 Concentrators; 2.2.3 Savant Speed Vac; 2.2.4 Salting Out; 2.3 Purification of Nucleic Acids; 2.3.1 Phenol-Chloroform Extraction
2.3.2 Precipitation Using Polyethylene Glycol2.3.3 Protein-Binding Filter Membranes; 2.3.4 Anion-Exchange Columns; 2.3.5 Glass Milk; 2.3.6 Cesium Chloride Density Gradient; 2.3.7 Dialysis; 2.4 Determining the Concentration of Nucleic Acid Solutions; 2.4.1 Optical Density Measurements with the Aid of Absorption Spectrometry; 2.4.2 Determination of Concentration by Means of Agarose Gels; 2.4.3 Dot Quantification; 2.4.4 Fluorometric Determination; 2.4.5 Nucleic Acid Dipsticks; 2.4.6 Enzymatic Evidence; 2.5 Methods of DNA Preparation; 2.5.1 Preparation of Plasmid DNA on a Small Scale 2.5.2 Preparation of Plasmid DNA on a Large Scale2.5.3 Bacterial Media; 2.5.4 Preparation of Phage DNA; 2.5.5 Preparation of Single-Stranded DNA with the Aid of Helper Phages; 2.5.6 Preparation of Genomic DNA; Chapter 3 The Tools; 3.1 Restriction Enzymes; 3.1.1 Nomenclature; 3.1.2 The Activity Test; 3.1.3 Making a Restriction Digestion; 3.1.4 Difficulties Associated with Restriction Digestion; 3.1.5 Works of Reference for Restriction Digestion; 3.2 Gels; 3.2.1 Agarose Gels; 3.2.2 Isolating DNA Fragments from Agarose Gels; 3.2.3 Polyacrylamide Gels 3.2.4 Isolating DNA Fragments from Polyacrylamide Gels3.2.5 Pulse-Field Gel Electrophoresis; 3.2.6 Capillary Electrophoresis; 3.3 Blotting; 3.3.1 Southern Blots; 3.3.2 Northern Blots; 3.3.3 Dot Blots and Slot Blots; Chapter 4 The Polymerase Chain Reaction; 4.1 Standard Polymerase Chain Reaction; 4.2 Suggestions for Improving the Polymerase Chain Reaction; 4.2.1 Nested Polymerase Chain Reaction; 4.2.2 Multiplex Polymerase Chain Reaction; 4.2.3 Amplification of Longer DNA Fragments; 4.3 PCR Applications; 4.3.1 Reverse Transcription-Polymerase Chain Reaction 4.3.2 Rapid Amplification of cDNA Ends4.3.3 Amplification of Coincidental Products; 4.3.4 Classic Quantitative Polymerase Chain Reaction; 4.3.5 Real-Time Quantitative Polymerase Chain Reaction; 4.3.6 Inverse Polymerase Chain Reaction; 4.3.7 Biotin-RAGE Method and Supported Polymerase Chain Reaction; 4.3.8 Mutagenesis with Modified Primers; 4.3.9 Amplification Refractory Mutation System; 4.3.10 In Situ Polymerase Chain Reaction; 4.3.11 Cycle Sequencing; 4.3.12 cDNA Synthesis; 4.3.13 Single-Cell Polymerase Chain Reaction; Chapter 5 RNA; 5.1 Inactivating RNases; 5.2 Methods of RNA Isolation 5.2.1 Single-Step Method |
| Record Nr. | UNINA-9910784550003321 |
|
Mülhardt Cornel
|
||
| Burlington, MA, : Academic Press, c2007 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Molecular biology and genomics [[electronic resource] /] / Cornel Mulhardt ; translated by E.W. Beese
| Molecular biology and genomics [[electronic resource] /] / Cornel Mulhardt ; translated by E.W. Beese |
| Autore | Mülhardt Cornel |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Burlington, MA, : Academic Press, c2007 |
| Descrizione fisica | 1 online resource (273 p.) |
| Disciplina | 572.8 |
| Collana | The experimenter series |
| Soggetto topico |
Molecular biology - Research - Methodology
Genomics - Research - Methodology |
| ISBN |
1-280-74685-8
9786610746859 0-08-046766-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Molecular Biology and Genomics; Copyright Page; Table of Contents; Foreword; Foreword to the First Edition; Acknowledgments; Chapter 1 What Is Molecular Biology?; 1.1 The Substrate of Molecular Biology: The Molecular World for Beginners; 1.2 What Is Required for This Work?; 1.3 Safety in the Laboratory; Chapter 2 Fundamental Methods; 2.1 Differences in Nucleic Acids; 2.2 Precipitation and Concentration of Nucleic Acids; 2.2.1 Alcohol Precipitation; 2.2.2 Concentrators; 2.2.3 Savant Speed Vac; 2.2.4 Salting Out; 2.3 Purification of Nucleic Acids; 2.3.1 Phenol-Chloroform Extraction
2.3.2 Precipitation Using Polyethylene Glycol2.3.3 Protein-Binding Filter Membranes; 2.3.4 Anion-Exchange Columns; 2.3.5 Glass Milk; 2.3.6 Cesium Chloride Density Gradient; 2.3.7 Dialysis; 2.4 Determining the Concentration of Nucleic Acid Solutions; 2.4.1 Optical Density Measurements with the Aid of Absorption Spectrometry; 2.4.2 Determination of Concentration by Means of Agarose Gels; 2.4.3 Dot Quantification; 2.4.4 Fluorometric Determination; 2.4.5 Nucleic Acid Dipsticks; 2.4.6 Enzymatic Evidence; 2.5 Methods of DNA Preparation; 2.5.1 Preparation of Plasmid DNA on a Small Scale 2.5.2 Preparation of Plasmid DNA on a Large Scale2.5.3 Bacterial Media; 2.5.4 Preparation of Phage DNA; 2.5.5 Preparation of Single-Stranded DNA with the Aid of Helper Phages; 2.5.6 Preparation of Genomic DNA; Chapter 3 The Tools; 3.1 Restriction Enzymes; 3.1.1 Nomenclature; 3.1.2 The Activity Test; 3.1.3 Making a Restriction Digestion; 3.1.4 Difficulties Associated with Restriction Digestion; 3.1.5 Works of Reference for Restriction Digestion; 3.2 Gels; 3.2.1 Agarose Gels; 3.2.2 Isolating DNA Fragments from Agarose Gels; 3.2.3 Polyacrylamide Gels 3.2.4 Isolating DNA Fragments from Polyacrylamide Gels3.2.5 Pulse-Field Gel Electrophoresis; 3.2.6 Capillary Electrophoresis; 3.3 Blotting; 3.3.1 Southern Blots; 3.3.2 Northern Blots; 3.3.3 Dot Blots and Slot Blots; Chapter 4 The Polymerase Chain Reaction; 4.1 Standard Polymerase Chain Reaction; 4.2 Suggestions for Improving the Polymerase Chain Reaction; 4.2.1 Nested Polymerase Chain Reaction; 4.2.2 Multiplex Polymerase Chain Reaction; 4.2.3 Amplification of Longer DNA Fragments; 4.3 PCR Applications; 4.3.1 Reverse Transcription-Polymerase Chain Reaction 4.3.2 Rapid Amplification of cDNA Ends4.3.3 Amplification of Coincidental Products; 4.3.4 Classic Quantitative Polymerase Chain Reaction; 4.3.5 Real-Time Quantitative Polymerase Chain Reaction; 4.3.6 Inverse Polymerase Chain Reaction; 4.3.7 Biotin-RAGE Method and Supported Polymerase Chain Reaction; 4.3.8 Mutagenesis with Modified Primers; 4.3.9 Amplification Refractory Mutation System; 4.3.10 In Situ Polymerase Chain Reaction; 4.3.11 Cycle Sequencing; 4.3.12 cDNA Synthesis; 4.3.13 Single-Cell Polymerase Chain Reaction; Chapter 5 RNA; 5.1 Inactivating RNases; 5.2 Methods of RNA Isolation 5.2.1 Single-Step Method |
| Record Nr. | UNINA-9910826866503321 |
|
Mülhardt Cornel
|
||
| Burlington, MA, : Academic Press, c2007 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Molecular biology of the cell : the problems book / John Wilson and Tim Hunt
| Molecular biology of the cell : the problems book / John Wilson and Tim Hunt |
| Autore | Wilson, John |
| Edizione | [6. ed] |
| Pubbl/distr/stampa | New York : Garland Science, c2015 |
| Descrizione fisica | XVI, 966 p. : ill. ; 28 cm. |
| Disciplina | 572.8(Biologia molecolare. Genetica molecolare. Genetica fisiologica. Acidi nucleici) |
| Altri autori (Persone) | Hunt, Tim |
| ISBN | 978-08-15-34453-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNICAMPANIA-SUN0118795 |
|
Wilson, John
|
||
| New York : Garland Science, c2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Vanvitelli | ||
| ||
Molecular biology of the cell : the problems book / John Wilson and Tim Hunt
| Molecular biology of the cell : the problems book / John Wilson and Tim Hunt |
| Autore | Wilson, John |
| Edizione | [6. ed] |
| Pubbl/distr/stampa | New York, : Garland Science, c2015 |
| Descrizione fisica | XVI, 966 p. : ill. ; 28 cm |
| Disciplina | 572.8(Biologia molecolare. Genetica molecolare. Genetica fisiologica. Acidi nucleici) |
| Altri autori (Persone) | Hunt, Tim |
| ISBN | 978-08-15-34453-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNICAMPANIA-VAN0118795 |
|
Wilson, John
|
||
| New York, : Garland Science, c2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Vanvitelli | ||
| ||
Molecular biology of the cell / Bruce Alberts ... [et al.] ; with problems by John Wilson, Tim Hunt
| Molecular biology of the cell / Bruce Alberts ... [et al.] ; with problems by John Wilson, Tim Hunt |
| Edizione | [6th ed.] |
| Pubbl/distr/stampa | New York : Garland Science, 2015 |
| Descrizione fisica | xxxiv, 1342 p., [88 p.] : ill. ; 28 cm |
| Disciplina | 572.8 |
| ISBN |
9780815344322
9780815344643 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910293958703321 |
| New York : Garland Science, 2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Molecular biology of the cell / Bruce Alberts ... [et al.]
| Molecular biology of the cell / Bruce Alberts ... [et al.] |
| Edizione | [6. ed] |
| Pubbl/distr/stampa | New York, : Garland Science, 2015 |
| Descrizione fisica | XXXIV, 1342 p. : ill. ; 28 cm. |
| Disciplina | 572.8(Biologia molecolare. Genetica molecolare. Genetica fisiologica. Acidi nucleici) |
| ISBN | 978-08-15-34464-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNICAMPANIA-SUN0118794 |
| New York, : Garland Science, 2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Vanvitelli | ||
| ||
Molecular biology of the cell / Bruce Alberts ... [et al.]
| Molecular biology of the cell / Bruce Alberts ... [et al.] |
| Edizione | [6. ed] |
| Pubbl/distr/stampa | New York, : Garland Science, 2015 |
| Descrizione fisica | XXXIV, 1342 p. : ill. ; 28 cm |
| Disciplina | 572.8(Biologia molecolare. Genetica molecolare. Genetica fisiologica. Acidi nucleici) |
| ISBN | 978-08-15-34464-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNICAMPANIA-VAN0118794 |
| New York, : Garland Science, 2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Vanvitelli | ||
| ||
