Mass spectrometry for lipidomics : methods and applications / / edited by Michal Holcapek and Kim Ekroos

• Pubblicazione: Weinheim, Germany : , : Wiley-VCH, , [2023]; ©2023
• Descrizione fisica: 1 online resource (750 pages)
• Disciplina: 574.19247
• Soggetto topico: Lipids - Analysis; Mass spectrometry
• Persona (resp. second.): HolčapekMichal <1971->; EkroosKim
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Cover -- Volume 1 -- Title Page -- Copyright Page -- Contents -- Preface -- Chapter 1 Introduction to Lipidomics -- 1.1 Preface -- 1.2 Historical Perspective -- 1.3 Sampling and Preanalytics -- 1.4 Reference Materials and Biological Reference Ranges -- 1.5 Clinical Lipidomics -- 1.6 Identification and Annotation -- 1.7 Quantitation -- 1.8 Lipid Ontology -- References -- Part I Analytical Methodologies in Lipidomics -- Chapter 2 Preanalytics for Lipidomics Analysis -- 2.1 Safety -- 2.2 Introduction -- 2.3 Sample Origin -- 2.4 Sample Collection -- 2.5 Tissue Homogenization -- 2.5.1 Mortar and Pestle -- 2.5.2 Rotor-Stator -- 2.5.3 Blender -- 2.5.4 Potter-Elvehjem -- 2.5.5 Bead Mill -- 2.6 Liquid-Liquid Extraction (LLE) -- 2.6.1 Folch Method -- 2.6.2 Bligh and Dyer (BD) Method -- 2.6.3 Modified Folch and Bligh/Dyer (BD) Methods -- 2.6.4 Rose and Oaklander (RO) Method -- 2.6.5 Matyash or Methyl-tert-Butyl Ether (MTBE) Method -- 2.6.6 BUME Method -- 2.6.7 Alshehry Method -- 2.6.8 Three-Phase Lipid Extraction (3PLE) -- 2.7 Resuspension and Solubilization -- 2.8 Automation -- 2.9 Tips and Tricks -- Acknowledgments -- References -- Chapter 3 Direct Infusion (Shotgun) Electrospray Mass Spectrometry -- 3.1 Introduction -- 3.2 Complexity of Crude Lipid Extracts -- 3.2.1 Main Lipid Classes in Mammalian Samples -- 3.2.2 Bond Types as Structural Features -- 3.2.3 Fatty Acids as the Major Building Blocks -- 3.2.4 Lipid Species and Double-Bond Series -- 3.3 Introduction to Mass Spectrometry of Lipids -- 3.3.1 Annotation of Lipid Structures Analyzed by MS -- 3.3.2 Isomers -- 3.3.3 Isobars and the Type-II Isotopic Overlap -- 3.4 Overview of Direct Infusion MS Workflows -- 3.5 Sample Preparation -- 3.5.1 Preanalytics - Sample Stability -- 3.5.2 Lipid Extraction -- 3.5.3 Solvents, Additives, and Lipid Concentration -- 3.5.4 Sample Derivatization. ; 3.6 Direct Infusion -- 3.7 Mass Spectrometry Analysis -- 3.7.1 Electrospray Ionization of Lipids -- 3.7.2 Tandem Mass Spectrometry -- 3.7.3 Multidimensional MS Shotgun Lipidomics -- 3.7.4 High-Resolution Mass Spectrometry -- 3.8 Lipid Identification -- 3.8.1 Identification by MS/MS -- 3.8.2 Identification by HRMS -- 3.8.3 Consideration of Type-II Overlap -- 3.8.4 Identification Hierarchy -- 3.8.5 Caveats/Pitfalls -- 3.9 Lipid Quantification -- 3.9.1 Internal Standards -- 3.9.2 Type-I Isotopic Effect -- 3.9.3 Evaluation and Correction of Isotopic Overlap -- 3.9.4 Species Response -- 3.9.5 Calculation of Concentration -- 3.10 Data Analysis/Software -- 3.11 Limitations -- 3.12 Selected Applications -- 3.12.1 Analysis of Plasma -- 3.12.2 Analysis of Tissues and Cells -- 3.12.3 Analysis of Lipid Metabolism -- 3.13 Outlook -- References -- Chapter 4 Liquid Chromatography - and Supercritical Fluid Chromatography - Mass Spectrometry -- 4.1 Introduction -- 4.2 Lipid Class Separation -- 4.2.1 Normal-Phase Liquid Chromatography -- 4.2.2 Hydrophilic Interaction Liquid Chromatography -- 4.2.3 Supercritical Fluid Chromatography -- 4.3 Lipid Species Separation -- 4.3.1 Reversed-Phase Liquid Chromatography -- 4.3.2 Nonaqueous Reversed-Phase Liquid Chromatography -- 4.4 Other Separation Approaches -- 4.4.1 Silver Ion Chromatography -- 4.4.2 Chiral Chromatography -- 4.4.3 Multidimensional Approaches -- References -- Chapter 5 Mass Spectrometry Imaging of Lipids -- 5.1 Introduction -- 5.2 SamplePreparation for Mass Spectrometry Imaging of Lipids -- 5.2.1 Tissue Samples -- 5.2.2 Sectioning and Mounting -- 5.2.3 Cell Culture -- 5.2.4 Pre-processing -- 5.2.5 Handling and Storage -- 5.2.6 Formalin-Fixed Paraffin-Embedded Tissue -- 5.3 Desorption/Ionization Techniques used for MSI of Lipids -- 5.3.1 Matrix-Assisted Laser Desorption/Ionization (MALDI). ; 5.3.2 Secondary Ion Mass Spectrometry SIMS -- 5.3.3 MSI Methods Using Electrospray Ionization -- 5.3.3.1 Desorption Electrospray Ionization -- 5.3.3.2 Laser Ablation Electrospray Ionization and IR-Matrix-Assisted Laser Desorption-Electrospray Ionization -- 5.3.3.3 Nanospray Desorption Electrospray Ionization -- 5.4 CombiningIon Mobility of Lipids with MSI -- 5.5 OnTissue Chemical Derivatization for MSI -- 5.6 Quantificationin MSI -- 5.7 LipidIdentification for MSI -- 5.7.1 Types of Ions Generated by MSI -- 5.7.2 In-source Fragmentation Considerations -- 5.7.3 MSI Lipid Identification Using Accurate Mass -- 5.7.4 Deploying MS/MS for Lipid Identification in MSI -- 5.7.5 Isomer-Resolved MSI -- 5.8 Conclusions -- References -- Chapter 6 Ion Mobility Spectrometry -- 6.1 Ion Mobility Spectrometry -- 6.1.1 Introduction -- 6.1.2 Ion Mobility Spectrometry Techniques and Platforms -- 6.1.2.1 Drift Tube Ion Mobility Spectrometry (DTIMS) -- 6.1.2.2 Traveling-WaveIon Mobility Spectrometry (TWIMS) -- 6.1.2.3 Trapped Ion Mobility Spectrometry (TIMS) -- 6.1.2.4 Field Asymmetric Ion Mobility Spectrometry (FAIMS) -- 6.1.3 Ion Mobility Resolving Power (Rp) Advancements -- 6.1.3.1 Cyclic IMS (cIM) -- 6.1.3.2 Standard Lossless Ion Manipulation (SLIM) -- 6.1.3.3 Tandem IMS -- 6.1.3.4 IMS Data Deconvolution Software Strategies -- 6.1.3.5 Drift Gas Dopants and Modifiers -- 6.1.4 Benefits of IMS for Lipidomics -- 6.1.4.1 Chemical Space Separation with IMS -- 6.1.4.2 Lipid Identification and Characterization with CCS -- 6.1.4.3 CCS for Lipid Structural Analysis -- 6.1.5 Lipidomic Applications with IMS -- 6.1.5.1 IMS in Imaging and Shotgun Lipidomics -- 6.1.5.2 IMS-MS/MS and Novel Speciation Approaches -- 6.1.6 Conclusions and Outlook of IMS for Lipidomics -- References -- Chapter 7 Structural Characterization of Lipids Using Advanced Mass Spectrometry Approaches. ; 7.1 Introduction -- 7.2 Structure and Position of Aliphatic Chains in Lipids -- 7.2.1 Double and Triple Bonds -- 7.2.1.1 Charge-Switch Derivatization of Fatty Acids -- 7.2.1.2 Ozone-Induced Dissociation -- 7.2.1.3 Paternò-Büchi Reaction -- 7.2.1.4 Epoxidation of Double Bonds -- 7.2.1.5 Acetonitrile-Related Adducts in APCI -- 7.2.1.6 Photodissociation of Unsaturated Lipids -- 7.2.1.7 Electron-Induced Dissociation of Unsaturated Lipids -- 7.2.2 Methyl Branching of Aliphatic Chains -- 7.2.3 Oxygen-Containing Functional Groups and Carbocyclic Structures -- 7.2.4 Stereospecific Position of Acyl Chain on Glycerol -- 7.3 Conclusions and Outlook -- References -- Chapter 8 Lipidomic Identification -- 8.1 Overview -- 8.2 Chromatography -- 8.3 Mass Spectrometry -- 8.3.1 Exact Mass -- 8.3.2 Fragment Spectra -- 8.3.2.1 General Considerations -- 8.3.2.2 Fatty Acids -- 8.3.2.3 Oxylipins -- 8.3.2.4 Phospholipids -- 8.3.2.5 Sphingolipids -- 8.3.2.6 Glycerolipids -- 8.3.2.7 Sterols -- 8.3.3 Deep Structure Determination -- 8.4 Ion Mobility Spectrometry -- 8.5 Identification Workflows -- References -- Chapter 9 Lipidomics Quantitation -- 9.1 Introduction to Lipidomics Quantitation -- 9.2 Principle of Quantitation -- 9.3 Internal Standards -- 9.4 Isotopic Correction -- 9.4.1 Isotopic Correction Type I -- 9.4.2 Isotopic Correction Type II -- 9.5 Common Approaches for Lipidomics Quantitation -- 9.5.1 Shotgun MS -- 9.5.2 Chromatography - MS -- 9.6 Validation -- 9.7 Quality Control (QC) -- References -- Chapter 10 The Past and Future of Lipidomics Bioinformatics -- 10.1 Introduction -- 10.2 A Modular Lipidomics Workflow -- 10.2.1 Data Formats -- 10.3 Targeted Lipidomics: Assay Design and Raw Data Analysis with LipidCreator and Skyline -- 10.4 Untargeted Lipidomics: Assay Design and Raw Data Analysis with LipidXplorer. ; 10.5 Standardization of Lipidomics Data with Goslin and lxPostman -- 10.6 Visualization and Lipidome Comparison with LUX Score and Beyond -- 10.7 Storage in Lipid Databases: What Is Currently There and What Should Be There -- 10.8 Outlook -- 10.8.1 Compatible Interfaces Between Modules -- 10.8.2 Quality Control -- 10.8.3 Reusability -- References -- Part II Lipidomic Analysis According to Lipid Categories and Classes -- Chapter 11 Fatty Acids: Structural and Quantitative Analysis -- Chapter 12 Quantitation of Oxylipins in Biological Samples, Focusing on Plasma, and Urine -- Chapter 13 Mass Spectrometry for Analysis of Glycerolipids -- Chapter 14 Lipidomic Analysis of Glycerophospholipid Molecular Species in Biological Samples -- Chapter 15 Sphingolipids -- Chapter 16 Sterol Lipids -- Chapter 17 Bile Acids -- Part III Lipidomic Applications -- Chapter 18 Lipidomic Profiling in a Large-Scale Cohort -- Chapter 19 Cancer Lipidomics - From the Perspective of Analytical Chemists -- Chapter 20 Lipidomics in Clinical Diagnostics -- Chapter 21 Lipidomics in Food Industry and Nutrition -- Chapter 22 Lipidomics in Plant Science -- Chapter 23 Lipidomics in Multi-Omics Studies -- Chapter 24 Tracer Lipidomics -- Chapter 25 Mass Spectrometry for Lipidomics: Methods and Applications - Aging and Alzheimer's Disease -- Chapter 26 Lipidomics in Cell Biology -- Chapter 27 Microbial Lipidomics -- Index -- Volume 2 -- Title Page -- Copyright Page -- Contents -- Preface -- Chapter 1 Introduction to Lipidomics -- Part I Analytical Methodologies in Lipidomics -- Chapter 2 Preanalytics for Lipidomics Analysis -- Chapter 3 Direct Infusion (Shotgun) Electrospray Mass Spectrometry -- Chapter 4 Liquid Chromatography - and Supercritical Fluid Chromatography - Mass Spectrometry -- Chapter 5 Mass Spectrometry Imaging of Lipids -- Chapter 6 Ion Mobility Spectrometry. ; Chapter 7 Structural Characterization of Lipids Using Advanced Mass Spectrometry Approaches.
• Titolo autorizzato: Mass spectrometry for lipidomics
• ISBN: 3-527-83651-9; 3-527-83649-7; 3-527-83650-0
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Lingua di pubblicazione: Inglese
• Record Nr.: 9910829831403321
• Lo trovi qui: Univ. Federico II