Biotechnology : a laboratory course / / Jeffrey M. Becker, Guy A. Caldwell, Eve Ann Zachgo
(Visualizza in formato marc)
(Visualizza in BIBFRAME)
| Autore: |
Becker Jeffrey M
|
| Titolo: |
Biotechnology : a laboratory course / / Jeffrey M. Becker, Guy A. Caldwell, Eve Ann Zachgo
|
| Pubblicazione: | San Diego, : Academic Press, c1990 |
| Descrizione fisica: | 1 online resource (251 p.) |
| Disciplina: | 660/.6 |
| Soggetto topico: | Biotechnology |
| Altri autori: |
CaldwellGuy A
ZachgoEve Ann
|
| Note generali: | Description based upon print version of record. |
| Nota di bibliografia: | Includes bibliographical references and index. |
| Nota di contenuto: | Front Cover; Biotechnology: A Laboratory Course; Copyright Page; Dedication; Table of Contents; Preface; Acknowledgments; Suggested Schedule for Exercises; Record Keeping and Safety Rules; Format of Student Laboratory Records; Ten Dos and Don'ts of Record Keeping; Criteria for Grading the Laboratory Notebook; Safety Rules in the Laboratory; Chapter 1. Measurement of pH; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 2. Use of Micropipettors and Spectrophotometers; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions |
| Chapter 3. Aseptic Technique: Transferring a CultureIntroduction; Reagents/Supplies; Equipment; Procedure; Questions; Chapter 4. Establishing a Pure Culture: The Streak Plate; Introduction; Reagents/Supplies; Equipment; Procedure; Questions; Chapter 5. Preparation of Culture Media; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 6. The Growth Curve; Introduction; Reagents/Supplies; Equipment; Procedure; Data Collection and Results; References; Questions; Chapter 7. Isolation of Plasmid DNA from Escherichia coli: The Mini-Prep; Introduction | |
| Reagents/SuppliesEquipment; Procedure; References; Questions; Chapter 8. Purification, Concentration, and Quantitation of DNA; Introduction; PART A PURIFICATION OF PLASMID DNA BY SOLVENT EXTRACTION; Reagents/Supplies; Equipment; Procedure; PART Β CONCENTRATION OF PLASMID DNA; Reagents/Supplies; Equipment; Procedure; PART C QUANTITATION OF DNA; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 9. Isolation of Plasmid DNA: The Maxi-Prep; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions | |
| Chapter 10. Restriction Digestion and Agarose Gel ElectrophoresisIntroduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 11. Southern Transfer; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 12. Preparation, Purification, and Hybridization of Probe; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 13. Transformation of Saccharomyces cerevisiae; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 14. Transformation of Escherichia coli by Plasmid DNA | |
| IntroductionReagents/Supplies; Equipment; Procedure; References; Questions; Chapter 15. Protein Assays; Introduction; PART A LOWRY PROTEIN ASSAY; Reagents/Supplies; Equipment; Procedure; PART Β BRADFORD PROTEIN ASSAY; Reagents/Supplies; Equipment; Procedure; References; Question; Chapter 16. β-Galaetosidase Assay; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 17. Determination of β-Galactosidase in Permeabilized Yeast Cells; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions | |
| Chapter 18. Assay of β-Galactosidase in Cell Extracts | |
| Sommario/riassunto: | Biotechnology |
| Titolo autorizzato: | Biotechnology |
| ISBN: | 0-323-15913-3 |
| 1-299-19350-1 | |
| Formato: | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione: | Inglese |
| Record Nr.: | 9910822601803321 |
| Lo trovi qui: | Univ. Federico II |
| Opac: | Controlla la disponibilità qui |