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Biomolecular archaeology [[electronic resource] /] / Terry Brown and Keri Brown
Biomolecular archaeology [[electronic resource] /] / Terry Brown and Keri Brown
Autore Brown T. A (Terence A.)
Pubbl/distr/stampa Chichester, West Sussex ; ; Malden, MA, : Wiley-Blackwell, 2011
Descrizione fisica 1 online resource (398 p.)
Disciplina 569.9
Altri autori (Persone) BrownKeri
Soggetto topico Biomolecular archaeology
Archaeology - Methodology
Soggetto genere / forma Electronic books.
ISBN 1-4443-9244-1
9786613407955
1-283-40795-7
1-4443-9242-5
1-4443-9243-3
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto ""Brief Contents""; ""Contents""; ""Halftitle page""; ""Title page""; ""Copyright""; ""List of Figures""; ""List of Tables""; ""Preface""; ""PART I BIOMOLECULES AND HOW THEY ARE STUDIED""; ""Chapter 1: What is Biomolecular Archaeology?""; ""1.1 The Scope of Biomolecular Archaeology""; ""1.2 Ancient and Modern Biomolecules""; ""1.3 The Challenges of Biomolecular Archaeology""; ""Chapter 2: DNA""; ""2.1 The Importance of DNA in Biomolecular Archaeology""; ""2.2 The Structure of DNA""; ""2.3 Genomes and Genes""; ""2.4 From Genomes to Organisms""; ""2.5 How Ancient DNA is Studied""
""Chapter 3: Proteins""""3.1 The Importance of Proteins in Biomolecular Archaeology""; ""3.2 Protein Structure and Synthesis""; ""3.3 Studying Proteins by Immunological Methods""; ""3.4 Studying Proteins by Proteomic Methods""; ""Chapter 4: Lipids""; ""4.1 The Structures of Lipids""; ""4.2 Methods for Studying Ancient Lipids""; ""Chapter 5: Carbohydrates""; ""5.1 The Structure of Carbohydrates""; ""5.2 Studying Starch Grains""; ""Chapter 6: Stable Isotopes""; ""6.1 Isotopes and Isotopic Fractionation""; ""6.2 Carbon and Nitrogen Isotope Fractionations Enable Past Human Diets to be Studied""
""6.3 Practical Aspects of Stable Isotope Studies""""PART II PRESERVATION AND DECAY OF BIOMOLECULES IN ARCHAEOLOGICAL SPECIMENS""; ""Chapter 7: Sources of Ancient Biomolecules""; ""7.1 Bones and Teeth""; ""7.2 Vertebrate Soft Tissues""; ""7.3 Plant Remains""; ""Chapter 8: Degradation of Ancient Biomolecules""; ""8.1 Complications in the Study of Biomolecular Degradation""; ""8.2 Degradation of Ancient DNA""; ""8.3 Degradation of Ancient Proteins""; ""8.4 Degradation of Ancient Lipids""; ""8.5 Degradation of Ancient Carbohydrates""
""Chapter 9: The Technical Challenges of Biomolecular Archaeology""""9.1 Problems Caused by Modern DNA Contamination""; ""9.2 Problems Caused by Overinterpretation of Data""; ""PART III THE APPLICATIONS OF BIOMOLECULAR ARCHAEOLOGY""; ""Chapter 10: Identifying the Sex of Human Remains""; ""10.1 The Archaeological Context to Human Sex Identification""; ""10.2 Osteological Approaches to Sex Identification""; ""10.3 Using DNA to Identify the Sex of Archaeological Skeletons""; ""10.4 Examples of the Application of Sex Identification in Biomolecular Archaeology""
""Chapter 11: Identifying the Kinship Relationships of Human Remains""""11.1 The Archaeological Context to Kinship Studies""; ""11.2 Using DNA to Study Kinship with Archaeological Skeletons""; ""11.3 Examples of the Application of Kinship Analysis in Biomolecular Archaeology""; ""Chapter 12: Studying the Diets of Past People""; ""12.1 The Archaeological Approach to Diet""; ""12.2 Studying Diet by Organic Residue Analysis and Stable Isotope Measurements""; ""12.3 Examples of the Use of Stable Isotope and Residue Analysis in Studies of Past Diet""; ""12.4 Using Genetics to Study Past Diets""
""Chapter 13: Studying the Origins and Spread of Agriculture""
Record Nr. UNINA-9910208826403321
Brown T. A (Terence A.)  
Chichester, West Sussex ; ; Malden, MA, : Wiley-Blackwell, 2011
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Gene cloning and DNA analysis : an introduction / / T.A. Brown
Gene cloning and DNA analysis : an introduction / / T.A. Brown
Autore Brown T. A (Terence A.)
Edizione [7th edition.]
Pubbl/distr/stampa Chichester : , : Wiley Blackwell, , 2016
Descrizione fisica 1 online resource (526 p.)
Disciplina 572.8/633
Soggetto topico Molecular cloning
Nucleotide sequence
DNA - Analysis
Soggetto genere / forma Electronic books.
ISBN 1-119-07254-9
1-119-07255-7
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910460927603321
Brown T. A (Terence A.)  
Chichester : , : Wiley Blackwell, , 2016
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Gene cloning and DNA analysis [[electronic resource]] : an introduction / / T.A. Brown
Gene cloning and DNA analysis [[electronic resource]] : an introduction / / T.A. Brown
Autore Brown T. A (Terence A.)
Edizione [6th ed.]
Pubbl/distr/stampa Hoboken, : Wiley-Blackwell, 2010
Descrizione fisica 1 online resource (338 p.)
Disciplina 572.8/633
Soggetto topico Molecular cloning
Nucleotide sequence
DNA - Analysis
Soggetto genere / forma Electronic books.
ISBN 1-282-68585-6
9786612685859
1-4443-1861-6
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto GENECLONINGAND DNAANALYSIS; Contents; TO THE SIXTH EDITION Preface to the Sixth Edition; PART IThe Basic Principlesof Gene Cloning andDNA Analysis; Chapter 1Why Gene Cloningand DNA Analysis areImportant; 1.1 The early development of genetics; 1.2 The advent of gene cloning and the polymerasechain reaction; 1.3 What is gene cloning?; 1.4 What is PCR?; 1.5 Why gene cloning and PCR are so important; 1.5.1 Obtaining a pure sample of a gene by cloning; 1.5.2 PCR can also be used to purify a gene; 1.6 How to find your way through this book
Chapter 2Vectors for GeneCloning:Plasmids andBacteriophages2.1 Plasmids; 2.1.1 Size and copy number; 2.1.2 Conjugation and compatibility; 2.1.3 Plasmid classification; 2.1.4 Plasmids in organisms other than bacteria; 2.2 Bacteriophages; 2.2.1 The phage infection cycle; 2.2.2 Lysogenic phages; Gene organization in the λ DNA molecule; The linear and circular forms of λ DNA; M13 - a filamentous phage; 2.2.3 Viruses as cloning vectors for other organisms; Chapter 3Purification of DNAfrom Living Cells; 3.1 Preparation of total cell DNA; 3.1.1 Growing and harvesting a bacterial culture
3.1.2 Preparation of a cell extract3.1.3 Purification of DNA from a cell extract; Removing contaminants by organic extraction and enzyme digestion; Using ion-exchange chromatography to purify DNA from a cell extract; 3.1.4 Concentration of DNA samples; 3.1.5 Measurement of DNA concentration; 3.1.6 Other methods for the preparation of total cell DNA; 3.2 Preparation of plasmid DNA; 3.2.1 Separation on the basis of size; 3.2.2 Separation on the basis of conformation; Alkaline denaturation; Ethidium bromide-caesium chloride density gradient centrifugation; 3.2.3 Plasmid amplification
3.3 Preparation of bacteriophage DNA3.3.1 Growth of cultures to obtain a high λ titer; 3.3.2 Preparation of non-lysogenic λ phages; 3.3.3 Collection of phages from an infected culture; 3.3.4 Purification of DNA from λ phage particles; 3.3.5 Purification of M13 DNA causes few problems; Chapter 4Manipulation ofPurified DNA; 4.1 The range of DNA manipulative enzymes; 4.1.1 Nucleases; 4.1.2 Ligases; 4.1.3 Polymerases; 4.1.4 DNA modifying enzymes; 4.2 Enzymes for cutting DNA - restriction endonucleases; 4.2.1 The discovery and function of restriction endonucleases
4.2.2 Type II restriction endonucleases cut DNA at specificnucleotide sequences4.2.3 Blunt ends and sticky ends; 4.2.4 The frequency of recognition sequences in a DNAmolecule; 4.2.5 Performing a restriction digest in the laboratory; 4.2.6 Analyzing the result of restriction endonucleasecleavage; Separation of molecules by gel electrophoresis; Visualizing DNA molecules in an agarose gel; 4.2.7 Estimation of the sizes of DNA molecules; 4.2.8 Mapping the positions of different restriction sites in aDNA molecule; 4.2.9 Special gel electrophoresis methods for separatinglarger molecules
4.3 Ligation - joining DNA molecules together
Record Nr. UNINA-9910458899903321
Brown T. A (Terence A.)  
Hoboken, : Wiley-Blackwell, 2010
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui