Pubbl/distr/stampa |
Hoboken, N.J., : John Wiley & Sons Inc., 2011
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Descrizione fisica |
1 online resource (380 p.)
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Disciplina |
571.6/381
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Altri autori (Persone) |
DavisJohn <1952->
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Collana |
Essential Methods
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Soggetto topico |
Cell culture - Technique
Tissue culture - Technique
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ISBN |
1-283-23936-1
9786613239365
0-470-66982-9
0-470-66981-0
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Formato |
Materiale a stampa ![](img/format/mas.png) |
Livello bibliografico |
Monografia |
Lingua di pubblicazione |
eng
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Nota di contenuto |
CONTENTS; Contributors; Preface; Abbreviations; Protocols; 1 The Cell Culture Laboratory; 1.1 Introduction; 1.2 Methods and approaches; 1.3 Troubleshooting; Acknowledgements; References; 2 Sterilization; 2.1 Introduction; 2.2 Methods and approaches; 2.3 Troubleshooting; References; 3 Microscopy of Living Cells; 3.1 Introduction; 3.2 Methods and approaches; 3.3 Troubleshooting; Acknowledgements; References; 4 Basic Techniques and Media, the Maintenance of Cell Lines,and Safety; 4.1 Introduction; 4.2 Methods and approaches; 4.3 Troubleshooting; Acknowledgements; References
5 Development and Optimization of Serum- and Protein-freeCulture Media5.1 Introduction; 5.2 Methods and approaches; 5.3 Troubleshooting; 5.4 Conclusion; Acknowledgements; References; 6 Cryopreservation and Banking of Cell Lines; 6.1 Introduction; 6.2 Methods and approaches; 6.3 Troubleshooting; Acknowledgements; References; 7 Primary Culture of Specific Cell Types and the Establishmentof Cell Lines; 7.1 Introduction; 7.2 Methods and approaches; 7.3 Troubleshooting; References; 8 Cloning; 8.1 Introduction; 8.2 Methods and approaches; 8.3 Troubleshooting; References
9 The Quality Control of Animal Cell Lines and the Prevention,Detection and Cure of Contamination9.1 Introduction; 9.2 Methods and approaches; 9.3 Troubleshooting; References; 10 Systems for Cell Culture Scale-up; 10.1 Introduction; 10.2 Methods and approaches; 10.3 Troubleshooting; References; 11 Good Laboratory Practice in the Cell Culture Laboratory; 11.1 Introduction; 11.2 Background to GLP; 11.3 General GLP principles; 11.4 Study performance; 11.5 Good Manufacturing Practice; 11.6 Summary; References; Index
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Record Nr. | UNINA-9910130871003321 |