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Production of recombinant proteins [[electronic resource] ] : novel microbial and eukaryotic expression systems / / edited by Gerd Gellissen
| Production of recombinant proteins [[electronic resource] ] : novel microbial and eukaryotic expression systems / / edited by Gerd Gellissen |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, c2005 |
| Descrizione fisica | 1 online resource (432 p.) |
| Disciplina | 660.63 |
| Altri autori (Persone) | GellissenGerd |
| Soggetto topico |
Recombinant proteins
Recombinant microorganisms Genetic vectors |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-280-51972-X
9786610519729 3-527-60367-0 3-527-60441-3 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Production of Recombinant Proteins; Preface; Foreword; Contents; List of Contributors; 1 Key and Criteria to the Selection of an Expression Platform; 2 Escherichia coli; 2.1 Introduction; 2.2 Strains, Genome, and Cultivation; 2.3 Expression Vectors; 2.3.1 Replication of pMB1-derived Vectors; 2.3.2 Plasmid Partitioning; 2.3.3 Genome Engineering; 2.3.4 E. coli Promoters; 2.4 Regulation of Gene Expression; 2.4.1 Negative Control; 2.4.2 Positive Control; 2.4.2.1 L-Arabinose Operon; 2.4.2.2 L-Rhamnose Operon; 2.5 Transcription and Translation; 2.5.1 Translation Initiation; 2.5.2 Codon Usage
2.5.3 Translation Termination2.5.4 Transcription Termination and mRNA Stability; 2.6 Protein Production; 2.6.1 Inclusion Body Formation; 2.6.1.1 Chaperones as Facilitators of Folding; 2.6.1.2 Fusion Protein Technology; 2.6.2 Methionine Processing; 2.6.3 Secretion into the Periplasm; 2.6.4 Disulfide Bond Formation and Folding; 2.6.5 Twin Arginine Translocation (TAT) of Folded Proteins; 2.6.6 Disulfide Bond Formation in the Cytoplasm; 2.6.7 Cell Surface Display and Secretion across the Outer Membrane; 2.7 Examples of Products and Processes; 2.8 Conclusions and Future Perspectives; Appendix References3 Pseudomonas fluorescens; 3.1 Introduction; 3.2 Biology of Pseudomonas fluorescens; 3.3 History and Taxonomy of Pseudomonas fluorescens Strain Biovar I MB101; 3.4 Cultivation; 3.5 Genomics and Functional Genomics of P. fluorescens Strain MB101; 3.6 Core Expression Platform for Heterologous Proteins; 3.6.1 Antibiotic-free Plasmids using pyrF and proC; 3.6.2 Gene Deletion Strategy and Re-usable Markers; 3.6.3 Periplasmic Secretion and Use of Transposomes; 3.6.4 Alternative Expression Systems: Anthranilate and Benzoate-inducible Promoters 3.7 Production of Heterologous Proteins in P. fluorescens3.7.1 Pharmaceutical Proteins; 3.7.2 Industrial Enzymes; 3.7.3 Agricultural Proteins; 3.8 Conclusions; Appendix; References; 4 Staphylococcus carnosus and other Gram-positive Bacteria; 4.1 Introduction; 4.2 Major Protein Export Routes in Gram-positive Bacteria; 4.2.1 The General Secretion (Sec) Pathway; 4.2.2 The Twin-Arginine Translocation (Tat) Pathway; 4.2.3 Secretion Signals; 4.3 Extracytosolic Protein Folding; 4.4 The Cell Wall as a Barrier for the Secretion of Heterologous Proteins 4.5 Degradation of Exported Proteins by Cell-associated and Secreted Proteases4.6 Staphylococcus carnosus; 4.6.1 General Description; 4.6.2 Microbiological and Molecular Biological Tools; 4.6.3 S. carnosus as Host Organism for the Analysis of Staphylococcal-related Pathogenicity Aspects; 4.6.4 Secretory Production of Heterologous Proteins by S. carnosus; 4.6.4.1 The Staphylococcus hyicus Lipase: Secretory Signals and Heterologous Expression in S. carnosus; 4.6.4.2 Use of the Pre-pro-part of the S. hyicus Lipase for the Secretion of Heterologous Proteins in S. carnosus 4.6.4.3 Process Development for the Secretory Production of a Human Calcitonin Precursor Fusion Protein by S. carnosus |
| Record Nr. | UNINA-9910144563203321 |
| Weinheim, : Wiley-VCH, c2005 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Production of recombinant proteins [[electronic resource] ] : novel microbial and eukaryotic expression systems / / edited by Gerd Gellissen
| Production of recombinant proteins [[electronic resource] ] : novel microbial and eukaryotic expression systems / / edited by Gerd Gellissen |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, c2005 |
| Descrizione fisica | 1 online resource (432 p.) |
| Disciplina | 660.63 |
| Altri autori (Persone) | GellissenGerd |
| Soggetto topico |
Recombinant proteins
Recombinant microorganisms Genetic vectors |
| ISBN |
1-280-51972-X
9786610519729 3-527-60367-0 3-527-60441-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Production of Recombinant Proteins; Preface; Foreword; Contents; List of Contributors; 1 Key and Criteria to the Selection of an Expression Platform; 2 Escherichia coli; 2.1 Introduction; 2.2 Strains, Genome, and Cultivation; 2.3 Expression Vectors; 2.3.1 Replication of pMB1-derived Vectors; 2.3.2 Plasmid Partitioning; 2.3.3 Genome Engineering; 2.3.4 E. coli Promoters; 2.4 Regulation of Gene Expression; 2.4.1 Negative Control; 2.4.2 Positive Control; 2.4.2.1 L-Arabinose Operon; 2.4.2.2 L-Rhamnose Operon; 2.5 Transcription and Translation; 2.5.1 Translation Initiation; 2.5.2 Codon Usage
2.5.3 Translation Termination2.5.4 Transcription Termination and mRNA Stability; 2.6 Protein Production; 2.6.1 Inclusion Body Formation; 2.6.1.1 Chaperones as Facilitators of Folding; 2.6.1.2 Fusion Protein Technology; 2.6.2 Methionine Processing; 2.6.3 Secretion into the Periplasm; 2.6.4 Disulfide Bond Formation and Folding; 2.6.5 Twin Arginine Translocation (TAT) of Folded Proteins; 2.6.6 Disulfide Bond Formation in the Cytoplasm; 2.6.7 Cell Surface Display and Secretion across the Outer Membrane; 2.7 Examples of Products and Processes; 2.8 Conclusions and Future Perspectives; Appendix References3 Pseudomonas fluorescens; 3.1 Introduction; 3.2 Biology of Pseudomonas fluorescens; 3.3 History and Taxonomy of Pseudomonas fluorescens Strain Biovar I MB101; 3.4 Cultivation; 3.5 Genomics and Functional Genomics of P. fluorescens Strain MB101; 3.6 Core Expression Platform for Heterologous Proteins; 3.6.1 Antibiotic-free Plasmids using pyrF and proC; 3.6.2 Gene Deletion Strategy and Re-usable Markers; 3.6.3 Periplasmic Secretion and Use of Transposomes; 3.6.4 Alternative Expression Systems: Anthranilate and Benzoate-inducible Promoters 3.7 Production of Heterologous Proteins in P. fluorescens3.7.1 Pharmaceutical Proteins; 3.7.2 Industrial Enzymes; 3.7.3 Agricultural Proteins; 3.8 Conclusions; Appendix; References; 4 Staphylococcus carnosus and other Gram-positive Bacteria; 4.1 Introduction; 4.2 Major Protein Export Routes in Gram-positive Bacteria; 4.2.1 The General Secretion (Sec) Pathway; 4.2.2 The Twin-Arginine Translocation (Tat) Pathway; 4.2.3 Secretion Signals; 4.3 Extracytosolic Protein Folding; 4.4 The Cell Wall as a Barrier for the Secretion of Heterologous Proteins 4.5 Degradation of Exported Proteins by Cell-associated and Secreted Proteases4.6 Staphylococcus carnosus; 4.6.1 General Description; 4.6.2 Microbiological and Molecular Biological Tools; 4.6.3 S. carnosus as Host Organism for the Analysis of Staphylococcal-related Pathogenicity Aspects; 4.6.4 Secretory Production of Heterologous Proteins by S. carnosus; 4.6.4.1 The Staphylococcus hyicus Lipase: Secretory Signals and Heterologous Expression in S. carnosus; 4.6.4.2 Use of the Pre-pro-part of the S. hyicus Lipase for the Secretion of Heterologous Proteins in S. carnosus 4.6.4.3 Process Development for the Secretory Production of a Human Calcitonin Precursor Fusion Protein by S. carnosus |
| Record Nr. | UNINA-9910831061403321 |
| Weinheim, : Wiley-VCH, c2005 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Production of recombinant proteins : novel microbial and eukaryotic expression systems / / edited by Gerd Gellissen
| Production of recombinant proteins : novel microbial and eukaryotic expression systems / / edited by Gerd Gellissen |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, c2005 |
| Descrizione fisica | 1 online resource (432 p.) |
| Disciplina | 660.63 |
| Altri autori (Persone) | GellissenGerd |
| Soggetto topico |
Recombinant proteins
Recombinant microorganisms Genetic vectors |
| ISBN |
1-280-51972-X
9786610519729 3-527-60367-0 3-527-60441-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Production of Recombinant Proteins; Preface; Foreword; Contents; List of Contributors; 1 Key and Criteria to the Selection of an Expression Platform; 2 Escherichia coli; 2.1 Introduction; 2.2 Strains, Genome, and Cultivation; 2.3 Expression Vectors; 2.3.1 Replication of pMB1-derived Vectors; 2.3.2 Plasmid Partitioning; 2.3.3 Genome Engineering; 2.3.4 E. coli Promoters; 2.4 Regulation of Gene Expression; 2.4.1 Negative Control; 2.4.2 Positive Control; 2.4.2.1 L-Arabinose Operon; 2.4.2.2 L-Rhamnose Operon; 2.5 Transcription and Translation; 2.5.1 Translation Initiation; 2.5.2 Codon Usage
2.5.3 Translation Termination2.5.4 Transcription Termination and mRNA Stability; 2.6 Protein Production; 2.6.1 Inclusion Body Formation; 2.6.1.1 Chaperones as Facilitators of Folding; 2.6.1.2 Fusion Protein Technology; 2.6.2 Methionine Processing; 2.6.3 Secretion into the Periplasm; 2.6.4 Disulfide Bond Formation and Folding; 2.6.5 Twin Arginine Translocation (TAT) of Folded Proteins; 2.6.6 Disulfide Bond Formation in the Cytoplasm; 2.6.7 Cell Surface Display and Secretion across the Outer Membrane; 2.7 Examples of Products and Processes; 2.8 Conclusions and Future Perspectives; Appendix References3 Pseudomonas fluorescens; 3.1 Introduction; 3.2 Biology of Pseudomonas fluorescens; 3.3 History and Taxonomy of Pseudomonas fluorescens Strain Biovar I MB101; 3.4 Cultivation; 3.5 Genomics and Functional Genomics of P. fluorescens Strain MB101; 3.6 Core Expression Platform for Heterologous Proteins; 3.6.1 Antibiotic-free Plasmids using pyrF and proC; 3.6.2 Gene Deletion Strategy and Re-usable Markers; 3.6.3 Periplasmic Secretion and Use of Transposomes; 3.6.4 Alternative Expression Systems: Anthranilate and Benzoate-inducible Promoters 3.7 Production of Heterologous Proteins in P. fluorescens3.7.1 Pharmaceutical Proteins; 3.7.2 Industrial Enzymes; 3.7.3 Agricultural Proteins; 3.8 Conclusions; Appendix; References; 4 Staphylococcus carnosus and other Gram-positive Bacteria; 4.1 Introduction; 4.2 Major Protein Export Routes in Gram-positive Bacteria; 4.2.1 The General Secretion (Sec) Pathway; 4.2.2 The Twin-Arginine Translocation (Tat) Pathway; 4.2.3 Secretion Signals; 4.3 Extracytosolic Protein Folding; 4.4 The Cell Wall as a Barrier for the Secretion of Heterologous Proteins 4.5 Degradation of Exported Proteins by Cell-associated and Secreted Proteases4.6 Staphylococcus carnosus; 4.6.1 General Description; 4.6.2 Microbiological and Molecular Biological Tools; 4.6.3 S. carnosus as Host Organism for the Analysis of Staphylococcal-related Pathogenicity Aspects; 4.6.4 Secretory Production of Heterologous Proteins by S. carnosus; 4.6.4.1 The Staphylococcus hyicus Lipase: Secretory Signals and Heterologous Expression in S. carnosus; 4.6.4.2 Use of the Pre-pro-part of the S. hyicus Lipase for the Secretion of Heterologous Proteins in S. carnosus 4.6.4.3 Process Development for the Secretory Production of a Human Calcitonin Precursor Fusion Protein by S. carnosus |
| Record Nr. | UNINA-9910877812503321 |
| Weinheim, : Wiley-VCH, c2005 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
