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Modification and Editing of RNA / / Henri Grosjean, Rob Benne
| Modification and Editing of RNA / / Henri Grosjean, Rob Benne |
| Autore | Grosjean Henri |
| Pubbl/distr/stampa | Hoboken, New Jersey : , : John Wiley & Sons, Inc., , 2014 |
| Descrizione fisica | 1 online resource (xiii, 596 pages) : illustrations |
| Disciplina | 572.88 |
| Soggetto topico | RNA editing |
| ISBN | 1-68367-268-2 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910830591603321 |
Grosjean Henri
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| Hoboken, New Jersey : , : John Wiley & Sons, Inc., , 2014 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Plant mitochondria : with emphasis on RNA editing and cytoplasmic male sterility / edited by A. Brennicke and U. Kück
| Plant mitochondria : with emphasis on RNA editing and cytoplasmic male sterility / edited by A. Brennicke and U. Kück |
| Pubbl/distr/stampa | Weinheim ; New York : VCH, c1993 |
| Descrizione fisica | 434 p. : ill. ; 25 cm |
| Disciplina | 581.83 |
| Altri autori (Persone) |
Brennicke, A.
Kück, H. U. |
| Soggetto topico |
Cytoplasmic male sterility
Plant mitochondria RNA editing |
| ISBN | 3527300333 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNISALENTO-991003542299707536 |
| Weinheim ; New York : VCH, c1993 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. del Salento | ||
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RNA and DNA editing [[electronic resource] ] : molecular mechanisms and their integration into biological systems / / edited by Harold C. Smith
| RNA and DNA editing [[electronic resource] ] : molecular mechanisms and their integration into biological systems / / edited by Harold C. Smith |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley-Interscience, c2008 |
| Descrizione fisica | 1 online resource (464 p.) |
| Disciplina | 572.8/8 |
| Altri autori (Persone) | SmithHarold C |
| Soggetto topico |
RNA editing
DNA Nucleotide sequence Genetic transcription |
| ISBN |
1-281-23753-1
9786611237530 0-470-26226-5 0-470-26225-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
RNA AND DNA EDITING; CONTENTS; PREFACE; ACKNOWLEDGMENTS; CONTRIBUTORS; PART I DIVERSIFICATION OF THE PROTEOME THROUGH RNA AND DNA EDITING; CHAPTER 1 DIVERSIFYING EXON CODE THROUGH A-TO-I RNA EDITING; 1.1 Introduction and Background; 1.1.1 Initial Discovery and Context of A-to-I RNA Editing and ADARs; 1.1.2 Important Cases of Recoding by A-to-I Modification in Pre-mRNA; 1.1.3 Cis-Acting Features for A-to-I Editing; 1.1.4 Properties of the A-to-I Editing Machinery; 1.2 Main Questions in the Field and Approaches; 1.2.1 Biochemical Versus Computational Approaches; 1.2.2 Editing of miRNA Sequences
1.3 Future Directions: Evolution of Editing Sites and MachineryReferences; CHAPTER 2 ANTIBODY GENE DIVERSIFICATION BY AID-CATALYZED DNA EDITING; 2.1 Introduction; 2.2 Before AID; 2.2.1 Without DNA (Darkness) and with DNA (Light); 2.2.2 Prominent Early Models for Antibody Diversification; 2.2.3 How Protein Sequencing Technology Enabled an Understanding of Antibody Diversity; 2.2.4 Somatic DNA Rearrangements Underpin V(D)J Joining and Create the Primary Antibody Repertoire; 2.2.5 Additional Antibody Diversity by Somatic Hypermutation (and Gene Conversion in Some Animals) 2.2.6 Altering Antibody Function by Class Switch Recombination (Isotype Switching)2.3 After AID; 2.3.1 A Novel Deaminase Is Required for CSR, SHM, and IGC; 2.3.2 AID Is a DNA Cytosine Deaminase that Directly Triggers Antibody Diversification; 2.3.3 The Importance of Uracil Bases in DNA In Vivo; 2.3.4 Processing of AID-induced Lesions: The Molecular Mechanism of Somatic-Hypermutation; 2.3.5 Processing of AID-induced Lesions: The Molecular Mechanism of Immunoglobulin Gene Conversion; 2.3.6 Processing of AID-induced Lesions: The Molecular Mechanism of Class Switch Recombination 2.4 Hot Areas and Speculations2.4.1 Immunodeficiency Syndromes Caused by Defects in AID-Mediated Ig Gene Diversification; 2.4.2 Regulating the DNA Mutator Activity of AID; 2.4.3 Misregulation of AID and Cancer; 2.4.4 AID Is But One Member of a Much Larger Family of Polynucleotide Deaminases; 2.5 Conclusions; Acknowledgments; References; CHAPTER 3 PROTEIN-PROTEIN AND RNA-PROTEIN INTERACTIONS IN U-INSERTION/DELETION RNA EDITING COMPLEXES; 3.1 A Bizarre Phenomenon and its Raison D'être; 3.2 The Catalytic Mechanism and Machinery 3.3 Extent of U-Insertion/Deletion RNA Editing in Trypanosoma and Leishmania Species3.4 Functional Studies of Editing Complex Subunits; 3.4.1 REN1, REN2, and MP67. Endonuclease Homologs; 3.4.2 REX1 and REX2. Exonuclease Homologs; 3.4.3 RET2. TUTase; 3.4.4 REL1 and REL2. Ligase Homologs; 3.4.5 MP81, MP63, MP42, MP46, MP44, MP24, MP18. Structural Components; 3.5 RNA-Protein Interactions: Isolated Subunits and Assembled Editing Complexes; 3.5.1 MP42; 3.5.2 MP24; 3.5.3 RNA-Protein Interactions in Assembled Editing Complexes; 3.6 Concluding Remarks; Acknowledgments; References CHAPTER 4 MACHINERY OF RNA EDITING IN PLANT ORGANELLES |
| Record Nr. | UNINA-9910145699603321 |
| Hoboken, N.J., : Wiley-Interscience, c2008 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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RNA and DNA editing : molecular mechanisms and their integration into biological systems / / edited by Harold C. Smith
| RNA and DNA editing : molecular mechanisms and their integration into biological systems / / edited by Harold C. Smith |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley-Interscience, c2008 |
| Descrizione fisica | 1 online resource (464 p.) |
| Disciplina | 572.8/8 |
| Altri autori (Persone) | SmithHarold C |
| Soggetto topico |
RNA editing
DNA Nucleotide sequence Genetic transcription |
| ISBN |
1-281-23753-1
9786611237530 0-470-26226-5 0-470-26225-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
RNA AND DNA EDITING; CONTENTS; PREFACE; ACKNOWLEDGMENTS; CONTRIBUTORS; PART I DIVERSIFICATION OF THE PROTEOME THROUGH RNA AND DNA EDITING; CHAPTER 1 DIVERSIFYING EXON CODE THROUGH A-TO-I RNA EDITING; 1.1 Introduction and Background; 1.1.1 Initial Discovery and Context of A-to-I RNA Editing and ADARs; 1.1.2 Important Cases of Recoding by A-to-I Modification in Pre-mRNA; 1.1.3 Cis-Acting Features for A-to-I Editing; 1.1.4 Properties of the A-to-I Editing Machinery; 1.2 Main Questions in the Field and Approaches; 1.2.1 Biochemical Versus Computational Approaches; 1.2.2 Editing of miRNA Sequences
1.3 Future Directions: Evolution of Editing Sites and MachineryReferences; CHAPTER 2 ANTIBODY GENE DIVERSIFICATION BY AID-CATALYZED DNA EDITING; 2.1 Introduction; 2.2 Before AID; 2.2.1 Without DNA (Darkness) and with DNA (Light); 2.2.2 Prominent Early Models for Antibody Diversification; 2.2.3 How Protein Sequencing Technology Enabled an Understanding of Antibody Diversity; 2.2.4 Somatic DNA Rearrangements Underpin V(D)J Joining and Create the Primary Antibody Repertoire; 2.2.5 Additional Antibody Diversity by Somatic Hypermutation (and Gene Conversion in Some Animals) 2.2.6 Altering Antibody Function by Class Switch Recombination (Isotype Switching)2.3 After AID; 2.3.1 A Novel Deaminase Is Required for CSR, SHM, and IGC; 2.3.2 AID Is a DNA Cytosine Deaminase that Directly Triggers Antibody Diversification; 2.3.3 The Importance of Uracil Bases in DNA In Vivo; 2.3.4 Processing of AID-induced Lesions: The Molecular Mechanism of Somatic-Hypermutation; 2.3.5 Processing of AID-induced Lesions: The Molecular Mechanism of Immunoglobulin Gene Conversion; 2.3.6 Processing of AID-induced Lesions: The Molecular Mechanism of Class Switch Recombination 2.4 Hot Areas and Speculations2.4.1 Immunodeficiency Syndromes Caused by Defects in AID-Mediated Ig Gene Diversification; 2.4.2 Regulating the DNA Mutator Activity of AID; 2.4.3 Misregulation of AID and Cancer; 2.4.4 AID Is But One Member of a Much Larger Family of Polynucleotide Deaminases; 2.5 Conclusions; Acknowledgments; References; CHAPTER 3 PROTEIN-PROTEIN AND RNA-PROTEIN INTERACTIONS IN U-INSERTION/DELETION RNA EDITING COMPLEXES; 3.1 A Bizarre Phenomenon and its Raison D'être; 3.2 The Catalytic Mechanism and Machinery 3.3 Extent of U-Insertion/Deletion RNA Editing in Trypanosoma and Leishmania Species3.4 Functional Studies of Editing Complex Subunits; 3.4.1 REN1, REN2, and MP67. Endonuclease Homologs; 3.4.2 REX1 and REX2. Exonuclease Homologs; 3.4.3 RET2. TUTase; 3.4.4 REL1 and REL2. Ligase Homologs; 3.4.5 MP81, MP63, MP42, MP46, MP44, MP24, MP18. Structural Components; 3.5 RNA-Protein Interactions: Isolated Subunits and Assembled Editing Complexes; 3.5.1 MP42; 3.5.2 MP24; 3.5.3 RNA-Protein Interactions in Assembled Editing Complexes; 3.6 Concluding Remarks; Acknowledgments; References CHAPTER 4 MACHINERY OF RNA EDITING IN PLANT ORGANELLES |
| Record Nr. | UNINA-9910807054603321 |
| Hoboken, N.J., : Wiley-Interscience, c2008 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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RNA editing : current research and future trends / / edited by Stefan Maas
| RNA editing : current research and future trends / / edited by Stefan Maas |
| Pubbl/distr/stampa | Norfolk, England : , : Caister Academic Press, , [2013] |
| Descrizione fisica | 1 online resource (249 p.) |
| Disciplina | 572.88 |
| Soggetto topico | RNA editing |
| Soggetto genere / forma | Electronic books. |
| ISBN | 1-908230-88-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Contents; Contributors; Preface; Ch 01: Regulation of Ion Channel and Transporter Function Through RNA Editing; Ch 02: Mechanisms and Functions of RNA Editing in Physarum polycephalum; Ch 03: Transfer RNA Modification and Editing; Ch 04: Coordination of RNA Editing with Other RNA Processes in Kinetoplastid Mitochondria; Ch 05: Structural Studies of U-Insertion/ Deletion RNA Editing in Trypanosomes; Ch 06: RNA Editing and Small Regulatory RNAs; Ch 07: Deaminase-dependent and Deaminase-independent Functionsof APOBEC1 and APOBEC1 Complementation Factor in the Context of the APOBEC Family
Ch 08: Identification of RNA Editing Sites: a Survey of the Past, Present and FutureCh 09: Regulation of Gene Expression Through Inosine-containing Untranslated Regions; ADAR and the Balance Game Between Virus Infection and InnateImmune Cell Response; Index |
| Record Nr. | UNINA-9910464348303321 |
| Norfolk, England : , : Caister Academic Press, , [2013] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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RNA editing : current research and future trends / / edited by Stefan Maas
| RNA editing : current research and future trends / / edited by Stefan Maas |
| Pubbl/distr/stampa | Norfolk, England : , : Caister Academic Press, , [2013] |
| Descrizione fisica | 1 online resource (249 p.) |
| Disciplina | 572.88 |
| Soggetto topico | RNA editing |
| ISBN | 1-908230-88-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Contents; Contributors; Preface; Ch 01: Regulation of Ion Channel and Transporter Function Through RNA Editing; Ch 02: Mechanisms and Functions of RNA Editing in Physarum polycephalum; Ch 03: Transfer RNA Modification and Editing; Ch 04: Coordination of RNA Editing with Other RNA Processes in Kinetoplastid Mitochondria; Ch 05: Structural Studies of U-Insertion/ Deletion RNA Editing in Trypanosomes; Ch 06: RNA Editing and Small Regulatory RNAs; Ch 07: Deaminase-dependent and Deaminase-independent Functionsof APOBEC1 and APOBEC1 Complementation Factor in the Context of the APOBEC Family
Ch 08: Identification of RNA Editing Sites: a Survey of the Past, Present and FutureCh 09: Regulation of Gene Expression Through Inosine-containing Untranslated Regions; ADAR and the Balance Game Between Virus Infection and InnateImmune Cell Response; Index |
| Record Nr. | UNINA-9910789573703321 |
| Norfolk, England : , : Caister Academic Press, , [2013] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
RNA editing : current research and future trends / / edited by Stefan Maas
| RNA editing : current research and future trends / / edited by Stefan Maas |
| Pubbl/distr/stampa | Norfolk, England : , : Caister Academic Press, , [2013] |
| Descrizione fisica | 1 online resource (249 p.) |
| Disciplina | 572.88 |
| Soggetto topico | RNA editing |
| ISBN | 1-908230-88-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Contents; Contributors; Preface; Ch 01: Regulation of Ion Channel and Transporter Function Through RNA Editing; Ch 02: Mechanisms and Functions of RNA Editing in Physarum polycephalum; Ch 03: Transfer RNA Modification and Editing; Ch 04: Coordination of RNA Editing with Other RNA Processes in Kinetoplastid Mitochondria; Ch 05: Structural Studies of U-Insertion/ Deletion RNA Editing in Trypanosomes; Ch 06: RNA Editing and Small Regulatory RNAs; Ch 07: Deaminase-dependent and Deaminase-independent Functionsof APOBEC1 and APOBEC1 Complementation Factor in the Context of the APOBEC Family
Ch 08: Identification of RNA Editing Sites: a Survey of the Past, Present and FutureCh 09: Regulation of Gene Expression Through Inosine-containing Untranslated Regions; ADAR and the Balance Game Between Virus Infection and InnateImmune Cell Response; Index |
| Record Nr. | UNINA-9910819225603321 |
| Norfolk, England : , : Caister Academic Press, , [2013] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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RNA interference from biology to therapeutics / / Kenneth A. Howard, editor
| RNA interference from biology to therapeutics / / Kenneth A. Howard, editor |
| Edizione | [1st ed. 2013.] |
| Pubbl/distr/stampa | New York, : Springer, 2013 |
| Descrizione fisica | 1 online resource (347 p.) |
| Disciplina | 572.88 |
| Altri autori (Persone) | HowardKenneth A |
| Collana | Advances in delivery science and technology |
| Soggetto topico |
RNA editing
Gene silencing |
| ISBN | 1-4614-4744-5 |
| Classificazione | WC 4460 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | RNA Interference Pathways and Therapeutic Exploitation RNA Interference -- Synthetic Dicer-Substrate siRNAs as Triggers of RNA Interference -- The Role of MicroRNAs in Natural Tissue Development and Application in Regenerative Medicine -- Intracellular Delivery Considerations for RNAi Therapeutics -- Mucosal Delivery of RNAi Therapeutics -- Nanomedicines for Systemic Delivery of RNAi Therapeutics -- Lipidoids: A Combinatorial Approach to siRNA Delivery -- Polymeric Micelles for siRNA Delivery -- RNAi Therapeutic Delivery by Exosomes -- Aptamer-Mediated siRNA Targeting -- RNAi as Anti-Viral Therapy: The HIV-1 Case -- Genome-Wide RNAi Screen for Identification of Human Host Factors Crucial for Influenza Virus Replication -- The Application of microRNAs in Cancer Diagnostics -- Therapeutic Application of miRNAs in Cancer -- Clinical Development of an Inhalable Anti-RSV siRNA Treatment. |
| Record Nr. | UNINA-9910437857203321 |
| New York, : Springer, 2013 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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RNA interference in practice [[electronic resource] /] / Ute Schepers
| RNA interference in practice [[electronic resource] /] / Ute Schepers |
| Autore | Schepers Ute |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, 2005 |
| Descrizione fisica | 1 online resource (338 p.) |
| Disciplina | 572.8845 |
| Soggetto topico |
RNA editing
Genetic transcription |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-280-51971-1
9786610519712 3-527-60439-1 3-527-60437-5 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
RNA Interference in Practice; Preface; Contents; 1 Introduction: RNA interference, the ""Breakthrough of the Year 2002""; 1.1 RNAi as a Tool for Functional Genomics; 1.2 Mechanism of RNAi; 1.3 Dicer - the Initiator to "Dice" the dsRNA?; 1.4 miRNAs versus siRNAs: Two Classes of Small RNAs Using the RNAi Pathway?; 1.5 RISC - the Effector to "Slice" the mRNA?; 1.6 Are RNA-Dependent RNA Polymerases (RdRps) Responsible for the Catalytic Nature of RNAi?; 1.7 Is RNAi Involved in the Regulation of Gene Expression?; 1.8 RNAi in Mammals; 1.9 Practical Approaches; 1.10 References
2 RNAi in Caenorhabditis elegans2.1 Introduction; 2.2 Application of RNAi in C. elegans; 2.3 Target Sequence Evaluation; 2.4 dsRNA Synthesis; 2.4.2 Generation of the DNA Template; 2.4.2.1 Plasmid Templates; 2.4.2.2 DNA Templates Derived by PCR/RT-PCR; 2.4.2 In-vitro dsRNA Transcription; 2.5 Delivery of dsRNA; 2.5.1 General Information on the C. elegans Anatomy; 2.5.2 C. elegans Strains for Silencing; 2.5.3 Culturing the Worms; 2.5.4 Microinjection Protocol; 2.5.5 Soaking Protocol; 2.5.6 RNAi Feeding Protocol; 2.5.7 DNA Templates for dsRNA Expression in Feeding E. coli 2.5.8 DNA Templates for Hairpin RNA Expression2.5.8.1 C. elegans Promoters; 2.5.8.2 Inverted Repeat Constructs; 2.6 Mounting Animals for Microscopy; 2.7 Genome Wide Screens; 2.7.1 C. elegans RNAi Library; 2.8 Selected Literature on C. elegans Research; 2.9 Useful C. elegans webpages; 2.10 References; 3 RNAi in Drosophila; 3.1 Introduction; 3.2 Application of RNAi in Drosophila; 3.2.1 dsRNA from Linear DNA Templates; 3.2.2 dsRNA from Inverted Repeat DNA; 3.2.3 Inducible Expression in Drosophila Cell Lines; 3.2.4 Limitations; 3.3 dsRNA Synthesis; 3.3.1 In-vitro dsRNA Transcription 3.3.2 Inverted Repeat DNA3.4 Injections; 3.4.1 Injection Services; 3.4.2 Injection Method; 3.4.3 DsRNA or Inverted Repeat DNA Preparation; 3.4.4 Embryo Collection; 3.5 Cell Lines; 3.6 Protocols; 3.6.1 Thawing and Maintenance of S2 Cells; 3.6.2 Freezing Protocol; 3.7 RNAi in S2 Cells; 3.7.1 dsRNA Transfection Using the Calcium Phosphate Method; 3.7.2 dsRNA Soaking of S2 Cells; 3.8 High-Throughput Screens; 3.8.1 Drosophila RNAi Library; 3.9 Useful Webpages for Drosophila Research; 3.10 Books and Literature on Drosophila; 3.11 References; 4 RNAi in Mammals; 4.1 Introduction 4.2 Transient RNAi in Cell Culture4.2.1 Chemical Synthesis and Modifications of siRNAs; 4.2.1.1 Advantages; 4.2.1.2 Limitations; 4.2.2 Custom Synthesis of siRNA Oligos; 4.2.3 siRNA Design Rules; 4.2.3.1 siRNA Strand Bias and Off-Gene Targeting; 4.2.3.2 Improvements in siRNA Stability; 4.2.3.3 siRNA Design: Novel Modifications of the "Tuschl Rules"; 4.2.3.4 Homology Search by BLAST, FASTA, or Smith-Waterman Algorithm; 4.2.3.5 Troubleshooting; 4.2.3.6 siRNA Design Programs and Algorithms; 4.2.3.7 Preparation of siRNA Duplexes; 4.2.4 Enzymatic Synthesis of siRNAs 4.2.4.1 Designing DNA Oligonucleotides |
| Record Nr. | UNINA-9910144557603321 |
|
Schepers Ute
|
||
| Weinheim, : Wiley-VCH, 2005 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
RNA interference in practice [[electronic resource] /] / Ute Schepers
| RNA interference in practice [[electronic resource] /] / Ute Schepers |
| Autore | Schepers Ute |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, 2005 |
| Descrizione fisica | 1 online resource (338 p.) |
| Disciplina | 572.8845 |
| Soggetto topico |
RNA editing
Genetic transcription |
| ISBN |
1-280-51971-1
9786610519712 3-527-60439-1 3-527-60437-5 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
RNA Interference in Practice; Preface; Contents; 1 Introduction: RNA interference, the ""Breakthrough of the Year 2002""; 1.1 RNAi as a Tool for Functional Genomics; 1.2 Mechanism of RNAi; 1.3 Dicer - the Initiator to "Dice" the dsRNA?; 1.4 miRNAs versus siRNAs: Two Classes of Small RNAs Using the RNAi Pathway?; 1.5 RISC - the Effector to "Slice" the mRNA?; 1.6 Are RNA-Dependent RNA Polymerases (RdRps) Responsible for the Catalytic Nature of RNAi?; 1.7 Is RNAi Involved in the Regulation of Gene Expression?; 1.8 RNAi in Mammals; 1.9 Practical Approaches; 1.10 References
2 RNAi in Caenorhabditis elegans2.1 Introduction; 2.2 Application of RNAi in C. elegans; 2.3 Target Sequence Evaluation; 2.4 dsRNA Synthesis; 2.4.2 Generation of the DNA Template; 2.4.2.1 Plasmid Templates; 2.4.2.2 DNA Templates Derived by PCR/RT-PCR; 2.4.2 In-vitro dsRNA Transcription; 2.5 Delivery of dsRNA; 2.5.1 General Information on the C. elegans Anatomy; 2.5.2 C. elegans Strains for Silencing; 2.5.3 Culturing the Worms; 2.5.4 Microinjection Protocol; 2.5.5 Soaking Protocol; 2.5.6 RNAi Feeding Protocol; 2.5.7 DNA Templates for dsRNA Expression in Feeding E. coli 2.5.8 DNA Templates for Hairpin RNA Expression2.5.8.1 C. elegans Promoters; 2.5.8.2 Inverted Repeat Constructs; 2.6 Mounting Animals for Microscopy; 2.7 Genome Wide Screens; 2.7.1 C. elegans RNAi Library; 2.8 Selected Literature on C. elegans Research; 2.9 Useful C. elegans webpages; 2.10 References; 3 RNAi in Drosophila; 3.1 Introduction; 3.2 Application of RNAi in Drosophila; 3.2.1 dsRNA from Linear DNA Templates; 3.2.2 dsRNA from Inverted Repeat DNA; 3.2.3 Inducible Expression in Drosophila Cell Lines; 3.2.4 Limitations; 3.3 dsRNA Synthesis; 3.3.1 In-vitro dsRNA Transcription 3.3.2 Inverted Repeat DNA3.4 Injections; 3.4.1 Injection Services; 3.4.2 Injection Method; 3.4.3 DsRNA or Inverted Repeat DNA Preparation; 3.4.4 Embryo Collection; 3.5 Cell Lines; 3.6 Protocols; 3.6.1 Thawing and Maintenance of S2 Cells; 3.6.2 Freezing Protocol; 3.7 RNAi in S2 Cells; 3.7.1 dsRNA Transfection Using the Calcium Phosphate Method; 3.7.2 dsRNA Soaking of S2 Cells; 3.8 High-Throughput Screens; 3.8.1 Drosophila RNAi Library; 3.9 Useful Webpages for Drosophila Research; 3.10 Books and Literature on Drosophila; 3.11 References; 4 RNAi in Mammals; 4.1 Introduction 4.2 Transient RNAi in Cell Culture4.2.1 Chemical Synthesis and Modifications of siRNAs; 4.2.1.1 Advantages; 4.2.1.2 Limitations; 4.2.2 Custom Synthesis of siRNA Oligos; 4.2.3 siRNA Design Rules; 4.2.3.1 siRNA Strand Bias and Off-Gene Targeting; 4.2.3.2 Improvements in siRNA Stability; 4.2.3.3 siRNA Design: Novel Modifications of the "Tuschl Rules"; 4.2.3.4 Homology Search by BLAST, FASTA, or Smith-Waterman Algorithm; 4.2.3.5 Troubleshooting; 4.2.3.6 siRNA Design Programs and Algorithms; 4.2.3.7 Preparation of siRNA Duplexes; 4.2.4 Enzymatic Synthesis of siRNAs 4.2.4.1 Designing DNA Oligonucleotides |
| Record Nr. | UNINA-9910830571903321 |
|
Schepers Ute
|
||
| Weinheim, : Wiley-VCH, 2005 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Soggetto topico
-
RNA editing
(13)
- Genetic transcription (5)
- Calcium channels (2)
- DNA (2)
- Neurosciences (2)