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Isolation of RNA from peripheral blood cells [[electronic resource] ] : a validation study for molecular diagnostics by microarray and kinetic RT-PCR assays -- application in aerospace medicine / / Nicole T. Vu ... [and others]
| Isolation of RNA from peripheral blood cells [[electronic resource] ] : a validation study for molecular diagnostics by microarray and kinetic RT-PCR assays -- application in aerospace medicine / / Nicole T. Vu ... [and others] |
| Pubbl/distr/stampa | Washington, DC : , : U.S. Dept. of Transportation, Federal Aviation Administration, Office of Aerospace Medicine, , [2004] |
| Altri autori (Persone) | VuNicole T |
| Soggetto topico |
RNA - Purification
Molecular diagnosis Protein microarrays Polymerase chain reaction Reverse transcriptase Aviation medicine - Technological innovations - United States |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Altri titoli varianti | Isolation of RNA from peripheral blood cells |
| Record Nr. | UNINA-9910693276203321 |
| Washington, DC : , : U.S. Dept. of Transportation, Federal Aviation Administration, Office of Aerospace Medicine, , [2004] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
RNA purification and analysis [[electronic resource] ] : sample preparation, extraction, chromatography / / by Douglas T. Gjerde, Lee Hoang and David Hornby
| RNA purification and analysis [[electronic resource] ] : sample preparation, extraction, chromatography / / by Douglas T. Gjerde, Lee Hoang and David Hornby |
| Autore | Gjerde Douglas T |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH |
| Descrizione fisica | 1 online resource (209 p.) |
| Disciplina |
572.88
572.8836 574.87/3283 |
| Altri autori (Persone) |
HornbyDavid, Dr.
HoangLee |
| Soggetto topico |
RNA - Analysis
RNA - Purification |
| ISBN |
1-282-68979-7
9786612689796 3-527-62719-7 3-527-62720-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
RNA Purification and Analysis: Sample Preparation, Extraction, Chromatography; Contents; Preface; Acknowledgments; 1: RNA Extraction, Separation, and Analysis; 1.1 The Need to Be Able to Extract, Manipulate, and Analyze RNA; 1.2 Using Chemical Tools to Solve the Problem of Analysis of Biological Processes; 1.3 The Principle of Chromatography and Solid-Phase Extraction; 1.3.1 Principle of Chromatography; 1.3.2 Mobile Phase Gradient Controls Elution; 1.3.3 Different Types of Column and Eluent Chemistries; 1.3.4 The Principle of Solid-Phase Extraction; 1.4 RNA Chromatography
1.5 Enzymatic Treatment of RNA and Analysis1.5.1 Polyacrylamide Gel Electrophoresis; 1.5.2 RNA Structure Probing with Ribonuclease Enzymes; 1.6 Content and Organization of This Book; References; 2: Biological and Chemical RNA; 2.1 Why Classify RNA with Biology and Chemistry?; 2.1.1 Chemical Classification of RNA; 2.1.2 Biological Classification of RNA; 2.2 Prokaryotic Cellular RNA; 2.3 Prokaryote Sample Type; 2.3.1 Escherichia coli; 2.3.2 Other Bacteria; 2.4 Eukaryotic Cellular RNA; 2.5 Eukaryote Sample Type; 2.5.1 Yeast; 2.5.2 Other Fungi; 2.5.3 Simple Multicellular Organism 2.5.4 Soft Animal2.5.5 Hard Animal; 2.5.6 Plant; 2.5.7 Cell Culture; 2.6 Other Samples; 2.6.1 Virus; 2.6.2 Soil and Rock; 2.7 Synthetic RNA; 2.7.1 Aptamers; 2.7.2 SELEX; 2.7.3 Short Hairpin RNAs; References; 3: RNA Separation: Substrates, Functional Groups, Mechanisms, and Control; 3.1 Solid-Phase Interaction; 3.1.1 Adsorption of Sample Compounds and Sample Matrix Compounds; 3.1.2 Roles of Solid-Phase Substrate and Functional Group; 3.1.3 Correlation of Interaction Type, Functional Group, and Substrate; 3.1.4 RNA Structure and Solid Surface Interaction 3.2 The Solid-Phase Substrate and Attachment of Functional Groups3.2.1 Polymeric Resin Substrates; 3.2.2 Porous and Nonporous Polymeric Resins; 3.2.3 Monolith Polymeric Columns; 3.2.4 Functionalization of the Polymer; 3.2.5 Silica-Glass-Based Substrates; 3.2.6 Functionalization of Silica; 3.2.7 Agarose and Cellulose Affinity Substrates; 3.2.8 Dextran and Polyacrylamide Gel Filtration Substrates; 3.3 Reverse-Phase Ion-Pairing Separation Mechanism; 3.4 Ion-Exchange Separation Mechanism; 3.5 Chaotropic Denaturing Interaction Mechanism; 3.6 Hybridization; 3.6.1 SELEX; 3.7 Gel Filtration References4: RNA Extraction and Analysis; 4.1 Transcription; 4.1.1 RNA Catalysis; 4.1.2 RNA-Protein Complex Interactions; 4.1.3 Pre-mRNA Splicing; 4.2 Translation; 4.2.1 Post-Transcriptional Control of Eukaryotic Gene Expression; 4.3 Gene Regulation; 4.3.1 RNA Interference Pathway; 4.3.2 Micro RNAs and Their Role in Gene Regulation; 4.4 Use of siRNA to Investigate Gene Function; References; 5: RNA Chromatography; 5.1 Development of RNA Chromatography; 5.2 RNA Chromatography Instrumentation; 5.2.1 The Column Oven; 5.2.2 Ultraviolet (UV) and Fluorescence Detection; 5.2.3 Fragment Collection 5.3 RNA Chromatography Conditions |
| Record Nr. | UNINA-9910139923703321 |
Gjerde Douglas T
|
||
| Weinheim, : Wiley-VCH | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
RNA purification and analysis [[electronic resource] ] : sample preparation, extraction, chromatography / / by Douglas T. Gjerde, Lee Hoang and David Hornby
| RNA purification and analysis [[electronic resource] ] : sample preparation, extraction, chromatography / / by Douglas T. Gjerde, Lee Hoang and David Hornby |
| Autore | Gjerde Douglas T |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH |
| Descrizione fisica | 1 online resource (209 p.) |
| Disciplina |
572.88
572.8836 574.87/3283 |
| Altri autori (Persone) |
HornbyDavid, Dr.
HoangLee |
| Soggetto topico |
RNA - Analysis
RNA - Purification |
| ISBN |
1-282-68979-7
9786612689796 3-527-62719-7 3-527-62720-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
RNA Purification and Analysis: Sample Preparation, Extraction, Chromatography; Contents; Preface; Acknowledgments; 1: RNA Extraction, Separation, and Analysis; 1.1 The Need to Be Able to Extract, Manipulate, and Analyze RNA; 1.2 Using Chemical Tools to Solve the Problem of Analysis of Biological Processes; 1.3 The Principle of Chromatography and Solid-Phase Extraction; 1.3.1 Principle of Chromatography; 1.3.2 Mobile Phase Gradient Controls Elution; 1.3.3 Different Types of Column and Eluent Chemistries; 1.3.4 The Principle of Solid-Phase Extraction; 1.4 RNA Chromatography
1.5 Enzymatic Treatment of RNA and Analysis1.5.1 Polyacrylamide Gel Electrophoresis; 1.5.2 RNA Structure Probing with Ribonuclease Enzymes; 1.6 Content and Organization of This Book; References; 2: Biological and Chemical RNA; 2.1 Why Classify RNA with Biology and Chemistry?; 2.1.1 Chemical Classification of RNA; 2.1.2 Biological Classification of RNA; 2.2 Prokaryotic Cellular RNA; 2.3 Prokaryote Sample Type; 2.3.1 Escherichia coli; 2.3.2 Other Bacteria; 2.4 Eukaryotic Cellular RNA; 2.5 Eukaryote Sample Type; 2.5.1 Yeast; 2.5.2 Other Fungi; 2.5.3 Simple Multicellular Organism 2.5.4 Soft Animal2.5.5 Hard Animal; 2.5.6 Plant; 2.5.7 Cell Culture; 2.6 Other Samples; 2.6.1 Virus; 2.6.2 Soil and Rock; 2.7 Synthetic RNA; 2.7.1 Aptamers; 2.7.2 SELEX; 2.7.3 Short Hairpin RNAs; References; 3: RNA Separation: Substrates, Functional Groups, Mechanisms, and Control; 3.1 Solid-Phase Interaction; 3.1.1 Adsorption of Sample Compounds and Sample Matrix Compounds; 3.1.2 Roles of Solid-Phase Substrate and Functional Group; 3.1.3 Correlation of Interaction Type, Functional Group, and Substrate; 3.1.4 RNA Structure and Solid Surface Interaction 3.2 The Solid-Phase Substrate and Attachment of Functional Groups3.2.1 Polymeric Resin Substrates; 3.2.2 Porous and Nonporous Polymeric Resins; 3.2.3 Monolith Polymeric Columns; 3.2.4 Functionalization of the Polymer; 3.2.5 Silica-Glass-Based Substrates; 3.2.6 Functionalization of Silica; 3.2.7 Agarose and Cellulose Affinity Substrates; 3.2.8 Dextran and Polyacrylamide Gel Filtration Substrates; 3.3 Reverse-Phase Ion-Pairing Separation Mechanism; 3.4 Ion-Exchange Separation Mechanism; 3.5 Chaotropic Denaturing Interaction Mechanism; 3.6 Hybridization; 3.6.1 SELEX; 3.7 Gel Filtration References4: RNA Extraction and Analysis; 4.1 Transcription; 4.1.1 RNA Catalysis; 4.1.2 RNA-Protein Complex Interactions; 4.1.3 Pre-mRNA Splicing; 4.2 Translation; 4.2.1 Post-Transcriptional Control of Eukaryotic Gene Expression; 4.3 Gene Regulation; 4.3.1 RNA Interference Pathway; 4.3.2 Micro RNAs and Their Role in Gene Regulation; 4.4 Use of siRNA to Investigate Gene Function; References; 5: RNA Chromatography; 5.1 Development of RNA Chromatography; 5.2 RNA Chromatography Instrumentation; 5.2.1 The Column Oven; 5.2.2 Ultraviolet (UV) and Fluorescence Detection; 5.2.3 Fragment Collection 5.3 RNA Chromatography Conditions |
| Record Nr. | UNINA-9910829855103321 |
|
Gjerde Douglas T
|
||
| Weinheim, : Wiley-VCH | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
RNA purification and analysis : sample preparation, extraction, chromatography / / by Douglas T. Gjerde, Lee Hoang and David Hornby
| RNA purification and analysis : sample preparation, extraction, chromatography / / by Douglas T. Gjerde, Lee Hoang and David Hornby |
| Autore | Gjerde Douglas T |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH |
| Descrizione fisica | 1 online resource (209 p.) |
| Disciplina |
572.88
572.8836 574.87/3283 |
| Altri autori (Persone) |
HornbyDavid, Dr.
HoangLee |
| Soggetto topico |
RNA - Analysis
RNA - Purification |
| ISBN |
1-282-68979-7
9786612689796 3-527-62719-7 3-527-62720-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
RNA Purification and Analysis: Sample Preparation, Extraction, Chromatography; Contents; Preface; Acknowledgments; 1: RNA Extraction, Separation, and Analysis; 1.1 The Need to Be Able to Extract, Manipulate, and Analyze RNA; 1.2 Using Chemical Tools to Solve the Problem of Analysis of Biological Processes; 1.3 The Principle of Chromatography and Solid-Phase Extraction; 1.3.1 Principle of Chromatography; 1.3.2 Mobile Phase Gradient Controls Elution; 1.3.3 Different Types of Column and Eluent Chemistries; 1.3.4 The Principle of Solid-Phase Extraction; 1.4 RNA Chromatography
1.5 Enzymatic Treatment of RNA and Analysis1.5.1 Polyacrylamide Gel Electrophoresis; 1.5.2 RNA Structure Probing with Ribonuclease Enzymes; 1.6 Content and Organization of This Book; References; 2: Biological and Chemical RNA; 2.1 Why Classify RNA with Biology and Chemistry?; 2.1.1 Chemical Classification of RNA; 2.1.2 Biological Classification of RNA; 2.2 Prokaryotic Cellular RNA; 2.3 Prokaryote Sample Type; 2.3.1 Escherichia coli; 2.3.2 Other Bacteria; 2.4 Eukaryotic Cellular RNA; 2.5 Eukaryote Sample Type; 2.5.1 Yeast; 2.5.2 Other Fungi; 2.5.3 Simple Multicellular Organism 2.5.4 Soft Animal2.5.5 Hard Animal; 2.5.6 Plant; 2.5.7 Cell Culture; 2.6 Other Samples; 2.6.1 Virus; 2.6.2 Soil and Rock; 2.7 Synthetic RNA; 2.7.1 Aptamers; 2.7.2 SELEX; 2.7.3 Short Hairpin RNAs; References; 3: RNA Separation: Substrates, Functional Groups, Mechanisms, and Control; 3.1 Solid-Phase Interaction; 3.1.1 Adsorption of Sample Compounds and Sample Matrix Compounds; 3.1.2 Roles of Solid-Phase Substrate and Functional Group; 3.1.3 Correlation of Interaction Type, Functional Group, and Substrate; 3.1.4 RNA Structure and Solid Surface Interaction 3.2 The Solid-Phase Substrate and Attachment of Functional Groups3.2.1 Polymeric Resin Substrates; 3.2.2 Porous and Nonporous Polymeric Resins; 3.2.3 Monolith Polymeric Columns; 3.2.4 Functionalization of the Polymer; 3.2.5 Silica-Glass-Based Substrates; 3.2.6 Functionalization of Silica; 3.2.7 Agarose and Cellulose Affinity Substrates; 3.2.8 Dextran and Polyacrylamide Gel Filtration Substrates; 3.3 Reverse-Phase Ion-Pairing Separation Mechanism; 3.4 Ion-Exchange Separation Mechanism; 3.5 Chaotropic Denaturing Interaction Mechanism; 3.6 Hybridization; 3.6.1 SELEX; 3.7 Gel Filtration References4: RNA Extraction and Analysis; 4.1 Transcription; 4.1.1 RNA Catalysis; 4.1.2 RNA-Protein Complex Interactions; 4.1.3 Pre-mRNA Splicing; 4.2 Translation; 4.2.1 Post-Transcriptional Control of Eukaryotic Gene Expression; 4.3 Gene Regulation; 4.3.1 RNA Interference Pathway; 4.3.2 Micro RNAs and Their Role in Gene Regulation; 4.4 Use of siRNA to Investigate Gene Function; References; 5: RNA Chromatography; 5.1 Development of RNA Chromatography; 5.2 RNA Chromatography Instrumentation; 5.2.1 The Column Oven; 5.2.2 Ultraviolet (UV) and Fluorescence Detection; 5.2.3 Fragment Collection 5.3 RNA Chromatography Conditions |
| Record Nr. | UNINA-9910876677603321 |
|
Gjerde Douglas T
|
||
| Weinheim, : Wiley-VCH | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
