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Mass spectrometry for microbial proteomics [[electronic resource] /] / edited by Haroun Shah and Saheer Gharbia
| Mass spectrometry for microbial proteomics [[electronic resource] /] / edited by Haroun Shah and Saheer Gharbia |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley, 2010 |
| Descrizione fisica | 1 online resource (535 p.) |
| Disciplina | 572/.36 |
| Altri autori (Persone) |
ShahHaroun N
GharbiaSaheer |
| Soggetto topico |
Microbial proteins - Spectra
Mass spectrometry Proteomics - Methodology |
| ISBN |
1-119-99192-7
1-282-65384-9 9786612653841 0-470-66549-1 0-470-66547-5 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | pt. 1. Microbial characterisation ; The transition from conventional methods to proteomics -- pt. 2. Proteomics tools and biomarker discovery -- pt. 3. Protein samples : preparation techniques -- pt. 4. Characterisation of microorganisms by pattern matching of mass spectral profiles and biomarker approaches requiring minimal sample preparation -- pt. 5. Targeted molecules and analysis of specific microorganisms -- pt. 6. Statistical analysis of 2D gels and analysis of mass spectral data -- pt. 7. DNA resequencing by MALDI-TOF-mass spectrometry and its application to traditional microbiological problems. |
| Record Nr. | UNINA-9910140555103321 |
| Hoboken, N.J., : Wiley, 2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Mass spectrometry for microbial proteomics / / edited by Haroun Shah and Saheer Gharbia
| Mass spectrometry for microbial proteomics / / edited by Haroun Shah and Saheer Gharbia |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley, 2010 |
| Descrizione fisica | 1 online resource (535 p.) |
| Disciplina | 572/.36 |
| Altri autori (Persone) |
ShahHaroun N
GharbiaSaheer |
| Soggetto topico |
Microbial proteins - Spectra
Mass spectrometry Proteomics - Methodology |
| ISBN |
9786612653841
9781119991922 1119991927 9781282653849 1282653849 9780470665497 0470665491 9780470665473 0470665475 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | pt. 1. Microbial characterisation ; The transition from conventional methods to proteomics -- pt. 2. Proteomics tools and biomarker discovery -- pt. 3. Protein samples : preparation techniques -- pt. 4. Characterisation of microorganisms by pattern matching of mass spectral profiles and biomarker approaches requiring minimal sample preparation -- pt. 5. Targeted molecules and analysis of specific microorganisms -- pt. 6. Statistical analysis of 2D gels and analysis of mass spectral data -- pt. 7. DNA resequencing by MALDI-TOF-mass spectrometry and its application to traditional microbiological problems. |
| Record Nr. | UNINA-9910827526003321 |
| Hoboken, N.J., : Wiley, 2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Methods in animal proteomics / / editors, P. David Eckersall, Phillip D. Whitfield
| Methods in animal proteomics / / editors, P. David Eckersall, Phillip D. Whitfield |
| Pubbl/distr/stampa | Chichester, West Sussex ; ; Ames, Iowa, : Wiley-Blackwell, 2011 |
| Descrizione fisica | 1 online resource (910 p.) |
| Disciplina |
636.089
636.0892398 |
| Altri autori (Persone) |
EckersallP. D
WhitfieldPhillip D |
| Soggetto topico |
Proteomics - Methodology
Veterinary medicine - Methodology |
| Soggetto genere / forma | Electronic books. |
| ISBN |
0-470-96064-7
0-470-96063-9 0-470-96066-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cover; Title page; Copyright page; Contributors; Acknowledgments; Section 1: Exploring Animal Proteomes; 1 An Introduction to Animal Proteomics; 1.1 Proteomics and Animal Systems; 1.2 Exploring Animal Proteomes; 1.3 Applications of Proteomics in Animal Systems; 2 Types of Sample and Experimental Planning; 2.1 Introduction; 2.2 Types of Samples and Their Collection; 2.3 General Preparation of Body Fluids, Cultured Cells in Suspension, and Blood; 2.4 Homogenization, Clarification, and Simplification; 2.5 Determination of Protein Concentration in Proteomic Samples; 2.6 Enzymatic Digestion
2.7 Experimental Design: Strategies for Simplification and Enrichment2.8 Specialized Techniques for Sample Preparation; 2.9 Experimental Planning and Design: General Guidelines; 2.10 Biomarker Discovery: Special Considerations for Experimental Planning; 2.11 Concluding Remarks; 3 Protein Separation Strategies; 3.1 Introduction; 3.2 "Classical" 2DE; 3.3 Other Varieties of 2DE; 3.4 Sample Complexity; 3.5 The Influence of Staining; 3.6 "Post-detection"; 3.7 Future Trends; 4 Methods and Approaches to Mass Spectroscopy-Based Protein Identification; 4.1 Introduction; 4.2 MS 4.3 Sample Preparation4.4 Protein Identification; 4.5 Linking Mass Spectra with Proteins; 4.6 Validation of MS Data; 4.7 Conclusions; 5 Bioinformatics in Animal Proteomics; 5.1 Introduction; 5.2 Methods; 5.3 Future Trends; 5.4 Sources of Further Information; 6 Comparative Proteomic Approaches; 6.1 Global Study of Proteins; 6.2 Approaches to Characterize the Proteome; 6.3 Quantitative Proteomics; 6.4 Bioinformatics and Biostatistics; 6.5 Future Prospects; 7 Advancing Technologies for Spatial and Temporal Proteomics; 7.1 Introduction; 7.2 Investigating the Spatial Proteome 7.3 Temporal Proteomics7.4 Case Study: Spatial and Temporal Profiling in the Chicken; 7.5 Combining Spatial and Temporal Proteomics; 7.6 Recent Developments in Animal Proteomics; 7.7 Future Trends; 7.8 Conclusions; 7.9 Sources of Further Information; Section 2: Applications of Proteomics in Animal Biology; 8 Proteomic Strategies to Investigate Adaptive Processes; 8.1 Introduction; 8.2 Hibernation Physiology and Behavior; 8.3 Sampling Strategy; 8.4 Sample Collection and Storage; 8.5 Design and Execution of a Quantitative Proteomic Experiment; 8.6 Conclusion 9 Investigation of Animal Venoms and Toxins9.1 Introduction; 9.2 Natural Bioresources; 9.3 Pharmaceutical Success Stories; 9.4 The Methods in Toxin Sequencing; 9.5 Reducing Complexity by Chromatography; 9.6 Bioassay Considerations; 9.7 Classical Edman; 9.8 Overview of MS Methods for Chemical Prospecting; 9.9 Soft Ionization Methods; 9.10 Common Mass Analyzers for Peptide Analysis; 9.11 Information Obtained from Different MS Systems; 9.12 Cloning Studies; 9.13 New Developments and Future Trends; 9.14 Conclusions; 9.15 Other Sources of Information; 10 Proteomics in Animal Health and Disease 10.1 Introduction |
| Record Nr. | UNINA-9910133839703321 |
| Chichester, West Sussex ; ; Ames, Iowa, : Wiley-Blackwell, 2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Methods in animal proteomics / / editors, P. David Eckersall, Phillip D. Whitfield
| Methods in animal proteomics / / editors, P. David Eckersall, Phillip D. Whitfield |
| Pubbl/distr/stampa | Chichester, West Sussex ; ; Ames, Iowa, : Wiley-Blackwell, 2011 |
| Descrizione fisica | 1 online resource (910 p.) |
| Disciplina |
636.089
636.0892398 |
| Altri autori (Persone) |
EckersallP. D
WhitfieldPhillip D |
| Soggetto topico |
Proteomics - Methodology
Veterinary medicine - Methodology |
| ISBN |
0-470-96064-7
0-470-96063-9 0-470-96066-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cover; Title page; Copyright page; Contributors; Acknowledgments; Section 1: Exploring Animal Proteomes; 1 An Introduction to Animal Proteomics; 1.1 Proteomics and Animal Systems; 1.2 Exploring Animal Proteomes; 1.3 Applications of Proteomics in Animal Systems; 2 Types of Sample and Experimental Planning; 2.1 Introduction; 2.2 Types of Samples and Their Collection; 2.3 General Preparation of Body Fluids, Cultured Cells in Suspension, and Blood; 2.4 Homogenization, Clarification, and Simplification; 2.5 Determination of Protein Concentration in Proteomic Samples; 2.6 Enzymatic Digestion
2.7 Experimental Design: Strategies for Simplification and Enrichment2.8 Specialized Techniques for Sample Preparation; 2.9 Experimental Planning and Design: General Guidelines; 2.10 Biomarker Discovery: Special Considerations for Experimental Planning; 2.11 Concluding Remarks; 3 Protein Separation Strategies; 3.1 Introduction; 3.2 "Classical" 2DE; 3.3 Other Varieties of 2DE; 3.4 Sample Complexity; 3.5 The Influence of Staining; 3.6 "Post-detection"; 3.7 Future Trends; 4 Methods and Approaches to Mass Spectroscopy-Based Protein Identification; 4.1 Introduction; 4.2 MS 4.3 Sample Preparation4.4 Protein Identification; 4.5 Linking Mass Spectra with Proteins; 4.6 Validation of MS Data; 4.7 Conclusions; 5 Bioinformatics in Animal Proteomics; 5.1 Introduction; 5.2 Methods; 5.3 Future Trends; 5.4 Sources of Further Information; 6 Comparative Proteomic Approaches; 6.1 Global Study of Proteins; 6.2 Approaches to Characterize the Proteome; 6.3 Quantitative Proteomics; 6.4 Bioinformatics and Biostatistics; 6.5 Future Prospects; 7 Advancing Technologies for Spatial and Temporal Proteomics; 7.1 Introduction; 7.2 Investigating the Spatial Proteome 7.3 Temporal Proteomics7.4 Case Study: Spatial and Temporal Profiling in the Chicken; 7.5 Combining Spatial and Temporal Proteomics; 7.6 Recent Developments in Animal Proteomics; 7.7 Future Trends; 7.8 Conclusions; 7.9 Sources of Further Information; Section 2: Applications of Proteomics in Animal Biology; 8 Proteomic Strategies to Investigate Adaptive Processes; 8.1 Introduction; 8.2 Hibernation Physiology and Behavior; 8.3 Sampling Strategy; 8.4 Sample Collection and Storage; 8.5 Design and Execution of a Quantitative Proteomic Experiment; 8.6 Conclusion 9 Investigation of Animal Venoms and Toxins9.1 Introduction; 9.2 Natural Bioresources; 9.3 Pharmaceutical Success Stories; 9.4 The Methods in Toxin Sequencing; 9.5 Reducing Complexity by Chromatography; 9.6 Bioassay Considerations; 9.7 Classical Edman; 9.8 Overview of MS Methods for Chemical Prospecting; 9.9 Soft Ionization Methods; 9.10 Common Mass Analyzers for Peptide Analysis; 9.11 Information Obtained from Different MS Systems; 9.12 Cloning Studies; 9.13 New Developments and Future Trends; 9.14 Conclusions; 9.15 Other Sources of Information; 10 Proteomics in Animal Health and Disease 10.1 Introduction |
| Record Nr. | UNINA-9910830213203321 |
| Chichester, West Sussex ; ; Ames, Iowa, : Wiley-Blackwell, 2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Methods in animal proteomics / / editors, P. David Eckersall, Phillip D. Whitfield
| Methods in animal proteomics / / editors, P. David Eckersall, Phillip D. Whitfield |
| Pubbl/distr/stampa | Chichester, West Sussex ; ; Ames, Iowa, : Wiley-Blackwell, 2011 |
| Descrizione fisica | 1 online resource (910 p.) |
| Disciplina | 636.089/2398 |
| Altri autori (Persone) |
EckersallP. D
WhitfieldPhillip D |
| Soggetto topico |
Proteomics - Methodology
Veterinary medicine - Methodology |
| ISBN |
9780470960646
0470960647 9780470960639 0470960639 9780470960660 0470960663 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cover; Title page; Copyright page; Contributors; Acknowledgments; Section 1: Exploring Animal Proteomes; 1 An Introduction to Animal Proteomics; 1.1 Proteomics and Animal Systems; 1.2 Exploring Animal Proteomes; 1.3 Applications of Proteomics in Animal Systems; 2 Types of Sample and Experimental Planning; 2.1 Introduction; 2.2 Types of Samples and Their Collection; 2.3 General Preparation of Body Fluids, Cultured Cells in Suspension, and Blood; 2.4 Homogenization, Clarification, and Simplification; 2.5 Determination of Protein Concentration in Proteomic Samples; 2.6 Enzymatic Digestion
2.7 Experimental Design: Strategies for Simplification and Enrichment2.8 Specialized Techniques for Sample Preparation; 2.9 Experimental Planning and Design: General Guidelines; 2.10 Biomarker Discovery: Special Considerations for Experimental Planning; 2.11 Concluding Remarks; 3 Protein Separation Strategies; 3.1 Introduction; 3.2 "Classical" 2DE; 3.3 Other Varieties of 2DE; 3.4 Sample Complexity; 3.5 The Influence of Staining; 3.6 "Post-detection"; 3.7 Future Trends; 4 Methods and Approaches to Mass Spectroscopy-Based Protein Identification; 4.1 Introduction; 4.2 MS 4.3 Sample Preparation4.4 Protein Identification; 4.5 Linking Mass Spectra with Proteins; 4.6 Validation of MS Data; 4.7 Conclusions; 5 Bioinformatics in Animal Proteomics; 5.1 Introduction; 5.2 Methods; 5.3 Future Trends; 5.4 Sources of Further Information; 6 Comparative Proteomic Approaches; 6.1 Global Study of Proteins; 6.2 Approaches to Characterize the Proteome; 6.3 Quantitative Proteomics; 6.4 Bioinformatics and Biostatistics; 6.5 Future Prospects; 7 Advancing Technologies for Spatial and Temporal Proteomics; 7.1 Introduction; 7.2 Investigating the Spatial Proteome 7.3 Temporal Proteomics7.4 Case Study: Spatial and Temporal Profiling in the Chicken; 7.5 Combining Spatial and Temporal Proteomics; 7.6 Recent Developments in Animal Proteomics; 7.7 Future Trends; 7.8 Conclusions; 7.9 Sources of Further Information; Section 2: Applications of Proteomics in Animal Biology; 8 Proteomic Strategies to Investigate Adaptive Processes; 8.1 Introduction; 8.2 Hibernation Physiology and Behavior; 8.3 Sampling Strategy; 8.4 Sample Collection and Storage; 8.5 Design and Execution of a Quantitative Proteomic Experiment; 8.6 Conclusion 9 Investigation of Animal Venoms and Toxins9.1 Introduction; 9.2 Natural Bioresources; 9.3 Pharmaceutical Success Stories; 9.4 The Methods in Toxin Sequencing; 9.5 Reducing Complexity by Chromatography; 9.6 Bioassay Considerations; 9.7 Classical Edman; 9.8 Overview of MS Methods for Chemical Prospecting; 9.9 Soft Ionization Methods; 9.10 Common Mass Analyzers for Peptide Analysis; 9.11 Information Obtained from Different MS Systems; 9.12 Cloning Studies; 9.13 New Developments and Future Trends; 9.14 Conclusions; 9.15 Other Sources of Information; 10 Proteomics in Animal Health and Disease 10.1 Introduction |
| Record Nr. | UNINA-9911019414603321 |
| Chichester, West Sussex ; ; Ames, Iowa, : Wiley-Blackwell, 2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Proteomics in cardiovascular disease : clinical considerations / / Christian Delles
| Proteomics in cardiovascular disease : clinical considerations / / Christian Delles |
| Autore | Delles Christian (Institute of Cardiovascular & Medical Sciences, University of Glasgow, UK) |
| Pubbl/distr/stampa | London, : Henry Stewart Talks, 2013 |
| Descrizione fisica | 1 online resource (1 streaming video file (34 min.) : color, sound) |
| Collana | Clinical proteomics |
| Soggetto topico |
Biochemical markers
Cardiovascular system - Diseases Proteomics - Methodology Biomarkers Cardiovascular Diseases - diagnosis Cardiovascular Diseases - physiopathology Proteome - analysis Proteomics - methods |
| Formato | Videoregistrazioni  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Contents: Systems biology -- Definition of proteomics -- Proteomics: rationale and practical aspects -- Proteomics in cardiovascular pathophysiology -- Proteomics to detect biomarkers of cardiovascular disease -- Urinary proteomics using capillary electrophoresis / mass spectrometry -- Proteomics of coronary artery disease -- Proteomics of pre-eclampsia -- Proteomics of other cardiovascular diseases: stroke, heart failure, diabetic nephropathy. |
| Record Nr. | UNINA-9910728789903321 |
Delles Christian (Institute of Cardiovascular & Medical Sciences, University of Glasgow, UK)
|
||
| London, : Henry Stewart Talks, 2013 | ||
| Videoregistrazioni | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Proteomics of biological systems [[electronic resource] ] : protein phosphorylation using mass spectrometry techniques / / Bryan M Ham
| Proteomics of biological systems [[electronic resource] ] : protein phosphorylation using mass spectrometry techniques / / Bryan M Ham |
| Autore | Ham Bryan M |
| Pubbl/distr/stampa | Hoboken, N.J., : John Wiley & Sons, 2012 |
| Descrizione fisica | 1 online resource (376 p.) |
| Disciplina | 572/.62 |
| Soggetto topico |
Proteomics - Methodology
Phosphorylation - Research - Methodology Phosphoproteins - Synthesis Mass spectrometry Biological systems - Research - Methodology |
| ISBN |
1-283-28285-2
9786613282859 1-118-13703-5 1-118-13704-3 1-118-13701-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
PROTEOMICS OF BIOLOGICAL SYSTEMS: Protein Phosphorylation Using Mass Spectrometry Techniques; CONTENTS; PREFACE; ACKNOWLEDGMENTS; ABOUT THE AUTHOR; 1: Posttranslational Modification (PTM) of Proteins; 1.1 OVER 200 FORMS OF PTM OF PROTEINS; 1.2 THREE MAIN TYPES OF PTM STUDIED BY MS; 1.3 OVERVIEW OF NANO-ELECTROSPRAY/NANOFLOW LC-MS; 1.3.1 Definition and Description of MS; 1.3.2 Basic Design of Mass Analyzer Instrumentation; 1.3.3 ESI; 1.3.4 Nano-ESI; 1.4 OVERVIEW OF NUCLEIC ACIDS; 1.5 PROTEINS AND PROTEOMICS; 1.5.1 Introduction to Proteomics; 1.5.2 Protein Structure and Chemistry
1.5.3 Bottom-Up Proteomics: MS of Peptides1.5.3.1 History and Strategy; 1.5.3.2 Protein Identification through Product Ion Spectra; 1.5.3.3 High-Energy Product Ions; 1.5.3.4 De Novo Sequencing; 1.5.3.5 Electron Capture Dissociation (ECD); 1.5.4 Top-Down Proteomics: MS of Intact Proteins; 1.5.4.1 Background; 1.5.4.2 GP Basicity and Protein Charging; 1.5.4.3 Calculation of Charge State and Molecular Weight; 1.5.4.4 Top-Down Protein Sequencing; 1.5.5 Systems Biology and Bioinformatics; 1.5.6 Biomarkers in Cancer; REFERENCES; 2: Glycosylation of Proteins; 2.1 PRODUCTION OF A GLYCOPROTEIN 2.2 BIOLOGICAL PROCESSES OF PROTEIN GLYCOSYLATION2.3 N-LINKED AND O-LINKED GLYCOSYLATION; 2.4 CARBOHYDRATES; 2.4.1 Ionization of Oligosaccharides; 2.4.2 Carbohydrate Fragmentation; 2.4.3 Complex Oligosaccharide Structural Elucidation; 2.5 THREE OBJECTIVES IN STUDYING GLYCOPROTEINS; 2.6 GLYCOSYLATION STUDY APPROACHES; 2.6.1 MS of Glycopeptides; 2.6.2 Mass Pattern Recognition; 2.6.2.1 High Galactose Glycosylation Pattern; 2.6.3 Charge State Determination; 2.6.4 Diagnostic Fragment Ions; 2.6.5 High-Resolution/High-Mass Accuracy Measurement and Identification; 2.6.6 Digested Bovine Fetuin REFERENCES3: Sulfation of Proteins as Posttranslational Modification; 3.1 GLYCOSAMINOGLYCAN SULFATION; 3.2 CELLULAR PROCESSES INVOLVED IN SULFATION; 3.3 BRIEF EXAMPLE OF PHOSPHORYLATION; 3.4 SULFOTRANSFERASE CLASS OF ENZYMES; 3.5 FRAGMENTATION NOMENCLATURE FOR CARBOHYDRATES; 3.6 SULFATED MUCIN OLIGOSACCHARIDES; 3.7 TYROSINE SULFATION; 3.8 TYROSYLPROTEIN SULFOTRANSFERASES TPST1 AND TPST2; 3.9 O-SULFATED HUMAN PROTEINS; 3.10 SULFATED PEPTIDE PRODUCT ION SPECTRA; 3.11 USE OF HIGHER ENERGY COLLISIONS; 3.12 ELECTRON CAPTURE DISSOCIATION (ECD); 3.13 SULFATION VERSUS PHOSPHORYLATION; REFERENCES 4: Eukaryote PTM as Phosphorylation: Normal State Studies4.1 MASS SPECTRAL MEASUREMENT WITH EXAMPLES OF HELA CELL PHOSPHOPROTEOME; 4.1.1 Introduction; 4.1.2 Protein Phosphatase and Kinase; 4.1.3 Hydroxy-Amino Acid Phosphorylation; 4.1.4 Traditional Phosphoproteomic Approaches; 4.1.5 Current Approaches; 4.1.5.1 Phosphoproteomic Enrichment Techniques; 4.1.5.2 IMAC; 4.1.5.3 MOAC; 4.1.5.4 Methylation of Peptides prior to IMAC or MOAC Enrichment; 4.1.6 The Ideal Approach; 4.1.7 One-Dimensional (1-D) Sodium Dodecyl Sulfate (SDS) PAGE; 4.1.8 Tandem MS Approach; 4.1.8.1 pS Loss of Phosphate Group 4.1.8.2 pT Loss of Phosphate Group |
| Record Nr. | UNINA-9910139593603321 |
|
Ham Bryan M
|
||
| Hoboken, N.J., : John Wiley & Sons, 2012 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Proteomics of biological systems : protein phosphorylation using mass spectrometry techniques / / Bryan M Ham
| Proteomics of biological systems : protein phosphorylation using mass spectrometry techniques / / Bryan M Ham |
| Autore | Ham Bryan M |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Hoboken, N.J., : John Wiley & Sons, 2012 |
| Descrizione fisica | 1 online resource (376 p.) |
| Disciplina | 572/.62 |
| Soggetto topico |
Proteomics - Methodology
Phosphorylation - Research - Methodology Phosphoproteins - Synthesis Mass spectrometry Biological systems - Research - Methodology |
| ISBN |
9786613282859
9781283282857 1283282852 9781118137031 1118137035 9781118137048 1118137043 9781118137017 1118137019 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
PROTEOMICS OF BIOLOGICAL SYSTEMS: Protein Phosphorylation Using Mass Spectrometry Techniques; CONTENTS; PREFACE; ACKNOWLEDGMENTS; ABOUT THE AUTHOR; 1: Posttranslational Modification (PTM) of Proteins; 1.1 OVER 200 FORMS OF PTM OF PROTEINS; 1.2 THREE MAIN TYPES OF PTM STUDIED BY MS; 1.3 OVERVIEW OF NANO-ELECTROSPRAY/NANOFLOW LC-MS; 1.3.1 Definition and Description of MS; 1.3.2 Basic Design of Mass Analyzer Instrumentation; 1.3.3 ESI; 1.3.4 Nano-ESI; 1.4 OVERVIEW OF NUCLEIC ACIDS; 1.5 PROTEINS AND PROTEOMICS; 1.5.1 Introduction to Proteomics; 1.5.2 Protein Structure and Chemistry
1.5.3 Bottom-Up Proteomics: MS of Peptides1.5.3.1 History and Strategy; 1.5.3.2 Protein Identification through Product Ion Spectra; 1.5.3.3 High-Energy Product Ions; 1.5.3.4 De Novo Sequencing; 1.5.3.5 Electron Capture Dissociation (ECD); 1.5.4 Top-Down Proteomics: MS of Intact Proteins; 1.5.4.1 Background; 1.5.4.2 GP Basicity and Protein Charging; 1.5.4.3 Calculation of Charge State and Molecular Weight; 1.5.4.4 Top-Down Protein Sequencing; 1.5.5 Systems Biology and Bioinformatics; 1.5.6 Biomarkers in Cancer; REFERENCES; 2: Glycosylation of Proteins; 2.1 PRODUCTION OF A GLYCOPROTEIN 2.2 BIOLOGICAL PROCESSES OF PROTEIN GLYCOSYLATION2.3 N-LINKED AND O-LINKED GLYCOSYLATION; 2.4 CARBOHYDRATES; 2.4.1 Ionization of Oligosaccharides; 2.4.2 Carbohydrate Fragmentation; 2.4.3 Complex Oligosaccharide Structural Elucidation; 2.5 THREE OBJECTIVES IN STUDYING GLYCOPROTEINS; 2.6 GLYCOSYLATION STUDY APPROACHES; 2.6.1 MS of Glycopeptides; 2.6.2 Mass Pattern Recognition; 2.6.2.1 High Galactose Glycosylation Pattern; 2.6.3 Charge State Determination; 2.6.4 Diagnostic Fragment Ions; 2.6.5 High-Resolution/High-Mass Accuracy Measurement and Identification; 2.6.6 Digested Bovine Fetuin REFERENCES3: Sulfation of Proteins as Posttranslational Modification; 3.1 GLYCOSAMINOGLYCAN SULFATION; 3.2 CELLULAR PROCESSES INVOLVED IN SULFATION; 3.3 BRIEF EXAMPLE OF PHOSPHORYLATION; 3.4 SULFOTRANSFERASE CLASS OF ENZYMES; 3.5 FRAGMENTATION NOMENCLATURE FOR CARBOHYDRATES; 3.6 SULFATED MUCIN OLIGOSACCHARIDES; 3.7 TYROSINE SULFATION; 3.8 TYROSYLPROTEIN SULFOTRANSFERASES TPST1 AND TPST2; 3.9 O-SULFATED HUMAN PROTEINS; 3.10 SULFATED PEPTIDE PRODUCT ION SPECTRA; 3.11 USE OF HIGHER ENERGY COLLISIONS; 3.12 ELECTRON CAPTURE DISSOCIATION (ECD); 3.13 SULFATION VERSUS PHOSPHORYLATION; REFERENCES 4: Eukaryote PTM as Phosphorylation: Normal State Studies4.1 MASS SPECTRAL MEASUREMENT WITH EXAMPLES OF HELA CELL PHOSPHOPROTEOME; 4.1.1 Introduction; 4.1.2 Protein Phosphatase and Kinase; 4.1.3 Hydroxy-Amino Acid Phosphorylation; 4.1.4 Traditional Phosphoproteomic Approaches; 4.1.5 Current Approaches; 4.1.5.1 Phosphoproteomic Enrichment Techniques; 4.1.5.2 IMAC; 4.1.5.3 MOAC; 4.1.5.4 Methylation of Peptides prior to IMAC or MOAC Enrichment; 4.1.6 The Ideal Approach; 4.1.7 One-Dimensional (1-D) Sodium Dodecyl Sulfate (SDS) PAGE; 4.1.8 Tandem MS Approach; 4.1.8.1 pS Loss of Phosphate Group 4.1.8.2 pT Loss of Phosphate Group |
| Record Nr. | UNINA-9910821480603321 |
|
Ham Bryan M
|
||
| Hoboken, N.J., : John Wiley & Sons, 2012 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
