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Biological activities of ribosome-inactivating proteins / / Lucía Citores, José M. Ferreras, editors
| Biological activities of ribosome-inactivating proteins / / Lucía Citores, José M. Ferreras, editors |
| Pubbl/distr/stampa | Basel : , : MDPI - Multidisciplinary Digital Publishing Institute, , [2023] |
| Descrizione fisica | 1 online resource (196 pages) |
| Disciplina | 574.8734 |
| Soggetto topico |
Ribosomes - Research
Proteins - Synthesis |
| ISBN | 3-0365-6823-9 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910683381003321 |
| Basel : , : MDPI - Multidisciplinary Digital Publishing Institute, , [2023] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Biophysics for therapeutic protein development / / Linda O. Narhi, editor
| Biophysics for therapeutic protein development / / Linda O. Narhi, editor |
| Edizione | [1st ed. 2013.] |
| Pubbl/distr/stampa | New York, : Springer, 2013 |
| Descrizione fisica | 1 online resource (297 p.) |
| Disciplina | 615.58 |
| Altri autori (Persone) | NarhiLinda O |
| Collana | Biophysics for the life sciences |
| Soggetto topico |
Proteins - Biotechnology
Cell culture Proteins - Synthesis Proteins - Therapeutic use Biophysics |
| ISBN |
1-299-40831-1
1-4614-4316-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Introduction -- High Throughput Biophysical Approaches to Therapeutic Protein Development -- Techniques for Higher Order Structure Determination -- Biophysical Techniques for Protein Size Distribution Analysis -- Qualification of biophysical methods for the analysis of protein therapeutics -- Application of Biophysics to the Early Developability Assessment of Therapeutic Candidates and its Application to Enhance Developability Properties -- Application of Biophysics in Formulation, Process and Product Characterization: Selected Case Studies -- Biophysical Analysis in Support of Development of Protein Pharmaceuticals -- Case Studies Applying Biophysical Techniques to Better Characterize Protein Aggregates and Particulates of Varying Size -- Investigation of Non Conformance During Protein Therapeutic Manufacturing -- Higher order structure and protein aggregate characterization of protein therapeutics: perspectives from good manufacturing practices and regulatory guidance -- Index. |
| Record Nr. | UNINA-9910437842603321 |
| New York, : Springer, 2013 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Cell-Free Protein Synthesis / / edited by Manish Biyani
| Cell-Free Protein Synthesis / / edited by Manish Biyani |
| Pubbl/distr/stampa | Rijeka : , : InTech, , 2012 |
| Descrizione fisica | 1 online resource (xi, 146 pages) : illustrations |
| Disciplina | 574.19296 |
| Soggetto topico | Proteins - Synthesis |
| ISBN | 953-51-5328-5 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910138450503321 |
| Rijeka : , : InTech, , 2012 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Cell-free protein synthesis [[electronic resource] ] : methods and protocols / / edited by Alexander S. Spirin and James R. Swartz
| Cell-free protein synthesis [[electronic resource] ] : methods and protocols / / edited by Alexander S. Spirin and James R. Swartz |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, c2008 |
| Descrizione fisica | 1 online resource (264 p.) |
| Disciplina | 572.6 |
| Altri autori (Persone) |
SpirinA. S (Aleksandr Sergeevich)
SwartzJames R |
| Soggetto topico |
Proteins - Synthesis
Genetic translation |
| Soggetto genere / forma | Electronic books. |
| ISBN |
3-527-69150-2
1-281-94699-0 9786611946999 3-527-62270-5 3-527-62269-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cell-free Protein Synthesis; Contents; Preface; List of Contributors; 1 Cell-free Protein Synthesis Systems: Historical Landmarks, Classification, and General Methods; 1.1 Introduction: Historical Landmarks; 1.1.1 Discovery of Protein Synthesis in Cell Extracts; 1.1.2 Translation of Exogenous Messages; 1.1.3 Coupled Transcription-translation in Bacterial Extracts; 1.1.4 Combined Transcription-translation Systems; 1.1.5 Continuous Flow/Continuous Exchange Principle; 1.2 Prokaryotic and Eukaryotic Types of Cell-free Expression Systems; 1.2.1 Cell Extracts; 1.2.1.1 E. coli extract (ECE)
1.2.1.2 Wheat Germ Extract (WGE)1.2.1.3 Rabbit Reticulocyte Lysate (RRL); 1.2.2 Genetic Constructs (Expression Vectors); 1.2.2.1 Prokaryotic Systems; 1.2.2.2 Eukaryotic Systems; 1.3 Preparing Cell Extracts; 1.3.1 E. coli Extracts; 1.3.1.1 Genetics; 1.3.1.2 Cell Growth; 1.3.1.3 Extract Preparation; 1.3.2 Wheat Germ Extracts; 1.4 Designing Reaction Composition; 1.4.1 Mg(2+) and Phosphate; 1.4.2 Other Salts; 1.4.3 Nucleotides and Amino Acids; 1.4.4 Stabilization Reagents; 1.4.5 Other Factors; 1.5 Providing Energy; 1.5.1 Direct Nucleotide Regeneration; 1.5.2 Indirect Nucleotide Regeneration 1.6 Enhancing Protein Folding1.6.1 Temperature Effects; 1.6.2 Cell Extract Concentration; 1.6.3 Effects of Folding Ligands; 1.6.4 Effects of Chaperones and Foldases; 1.6.5 Effects of Detergents; 2 The Constructive Approach for Cell-free Translation; 2.1 Introduction; 2.2 The Process of Protein Synthesis; 2.2.1 Polypeptide Synthesis; 2.2.2 Protein Maturation; 2.3 A Constructive Approach to Protein Synthesis; 2.3.1 In Vitro Reconstitution of Polypeptide Synthesis; 2.3.2 Protocol of Protein Synthesis using PURE System; 2.3.3 Addition of Protein Folding Machinery to the PURE System 2.3.4 Integration of a Membrane Targeting System with the PURE system2.3.5 Protein Synthesis using the PURE System containing Molecular Chaperones; 2.4 Conclusion; 3 Functional Genomic Analysis using Sequential Cell-free Protein Synthesis; 3.1 Introduction; 3.1.1 The Post-genomic Era; 3.1.2 Cell-free Protein Synthesis (CFPS) as a Functional Proteomic Tool; 3.2 Developing an enabling Technology for Sequential Expression Analysis; 3.2.1 Improving Linear Template Stability; 3.2.2 Improving PCR Reactions for generating Genomic Linear Templates 3.2.3 Optimizing Cofactor Concentrations for Enzyme Activation3.3 Demonstrating Functional Genomic Analysis with CFPS; 3.3.1 Isolation and Expression of Genomic Targets; 3.3.2 Effects of Sample Library on β-Lactamase Expression and Activity; 3.4 Conclusions and Projections; 4 Cell-free Technology for Rapid Production of Patient-specific Fusion Protein Vaccines; 4.1 Introduction; 4.1.1 Lymphoma and Fusion Protein Vaccine Treatments; 4.1.2 Comparing Cell-free and In Vivo Production Systems; 4.2 Developing the Fusion Protein Construct and the Cell-free Production Process 4.2.1 Fusion-protein Production in the Cell-free System |
| Record Nr. | UNINA-9910144101803321 |
| Weinheim, : Wiley-VCH, c2008 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Cell-free protein synthesis [[electronic resource] ] : methods and protocols / / edited by Alexander S. Spirin and James R. Swartz
| Cell-free protein synthesis [[electronic resource] ] : methods and protocols / / edited by Alexander S. Spirin and James R. Swartz |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, c2008 |
| Descrizione fisica | 1 online resource (264 p.) |
| Disciplina | 572.6 |
| Altri autori (Persone) |
SpirinA. S (Aleksandr Sergeevich)
SwartzJames R |
| Soggetto topico |
Proteins - Synthesis
Genetic translation |
| ISBN |
3-527-69150-2
1-281-94699-0 9786611946999 3-527-62270-5 3-527-62269-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cell-free Protein Synthesis; Contents; Preface; List of Contributors; 1 Cell-free Protein Synthesis Systems: Historical Landmarks, Classification, and General Methods; 1.1 Introduction: Historical Landmarks; 1.1.1 Discovery of Protein Synthesis in Cell Extracts; 1.1.2 Translation of Exogenous Messages; 1.1.3 Coupled Transcription-translation in Bacterial Extracts; 1.1.4 Combined Transcription-translation Systems; 1.1.5 Continuous Flow/Continuous Exchange Principle; 1.2 Prokaryotic and Eukaryotic Types of Cell-free Expression Systems; 1.2.1 Cell Extracts; 1.2.1.1 E. coli extract (ECE)
1.2.1.2 Wheat Germ Extract (WGE)1.2.1.3 Rabbit Reticulocyte Lysate (RRL); 1.2.2 Genetic Constructs (Expression Vectors); 1.2.2.1 Prokaryotic Systems; 1.2.2.2 Eukaryotic Systems; 1.3 Preparing Cell Extracts; 1.3.1 E. coli Extracts; 1.3.1.1 Genetics; 1.3.1.2 Cell Growth; 1.3.1.3 Extract Preparation; 1.3.2 Wheat Germ Extracts; 1.4 Designing Reaction Composition; 1.4.1 Mg(2+) and Phosphate; 1.4.2 Other Salts; 1.4.3 Nucleotides and Amino Acids; 1.4.4 Stabilization Reagents; 1.4.5 Other Factors; 1.5 Providing Energy; 1.5.1 Direct Nucleotide Regeneration; 1.5.2 Indirect Nucleotide Regeneration 1.6 Enhancing Protein Folding1.6.1 Temperature Effects; 1.6.2 Cell Extract Concentration; 1.6.3 Effects of Folding Ligands; 1.6.4 Effects of Chaperones and Foldases; 1.6.5 Effects of Detergents; 2 The Constructive Approach for Cell-free Translation; 2.1 Introduction; 2.2 The Process of Protein Synthesis; 2.2.1 Polypeptide Synthesis; 2.2.2 Protein Maturation; 2.3 A Constructive Approach to Protein Synthesis; 2.3.1 In Vitro Reconstitution of Polypeptide Synthesis; 2.3.2 Protocol of Protein Synthesis using PURE System; 2.3.3 Addition of Protein Folding Machinery to the PURE System 2.3.4 Integration of a Membrane Targeting System with the PURE system2.3.5 Protein Synthesis using the PURE System containing Molecular Chaperones; 2.4 Conclusion; 3 Functional Genomic Analysis using Sequential Cell-free Protein Synthesis; 3.1 Introduction; 3.1.1 The Post-genomic Era; 3.1.2 Cell-free Protein Synthesis (CFPS) as a Functional Proteomic Tool; 3.2 Developing an enabling Technology for Sequential Expression Analysis; 3.2.1 Improving Linear Template Stability; 3.2.2 Improving PCR Reactions for generating Genomic Linear Templates 3.2.3 Optimizing Cofactor Concentrations for Enzyme Activation3.3 Demonstrating Functional Genomic Analysis with CFPS; 3.3.1 Isolation and Expression of Genomic Targets; 3.3.2 Effects of Sample Library on β-Lactamase Expression and Activity; 3.4 Conclusions and Projections; 4 Cell-free Technology for Rapid Production of Patient-specific Fusion Protein Vaccines; 4.1 Introduction; 4.1.1 Lymphoma and Fusion Protein Vaccine Treatments; 4.1.2 Comparing Cell-free and In Vivo Production Systems; 4.2 Developing the Fusion Protein Construct and the Cell-free Production Process 4.2.1 Fusion-protein Production in the Cell-free System |
| Record Nr. | UNINA-9910829884203321 |
| Weinheim, : Wiley-VCH, c2008 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Cell-free protein synthesis : methods and protocols / / edited by Alexander S. Spirin and James R. Swartz
| Cell-free protein synthesis : methods and protocols / / edited by Alexander S. Spirin and James R. Swartz |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, c2008 |
| Descrizione fisica | 1 online resource (264 p.) |
| Disciplina | 572.6 |
| Altri autori (Persone) |
SpirinA. S (Aleksandr Sergeevich)
SwartzJames R |
| Soggetto topico |
Proteins - Synthesis
Genetic translation |
| ISBN |
3-527-69150-2
1-281-94699-0 9786611946999 3-527-62270-5 3-527-62269-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cell-free Protein Synthesis; Contents; Preface; List of Contributors; 1 Cell-free Protein Synthesis Systems: Historical Landmarks, Classification, and General Methods; 1.1 Introduction: Historical Landmarks; 1.1.1 Discovery of Protein Synthesis in Cell Extracts; 1.1.2 Translation of Exogenous Messages; 1.1.3 Coupled Transcription-translation in Bacterial Extracts; 1.1.4 Combined Transcription-translation Systems; 1.1.5 Continuous Flow/Continuous Exchange Principle; 1.2 Prokaryotic and Eukaryotic Types of Cell-free Expression Systems; 1.2.1 Cell Extracts; 1.2.1.1 E. coli extract (ECE)
1.2.1.2 Wheat Germ Extract (WGE)1.2.1.3 Rabbit Reticulocyte Lysate (RRL); 1.2.2 Genetic Constructs (Expression Vectors); 1.2.2.1 Prokaryotic Systems; 1.2.2.2 Eukaryotic Systems; 1.3 Preparing Cell Extracts; 1.3.1 E. coli Extracts; 1.3.1.1 Genetics; 1.3.1.2 Cell Growth; 1.3.1.3 Extract Preparation; 1.3.2 Wheat Germ Extracts; 1.4 Designing Reaction Composition; 1.4.1 Mg(2+) and Phosphate; 1.4.2 Other Salts; 1.4.3 Nucleotides and Amino Acids; 1.4.4 Stabilization Reagents; 1.4.5 Other Factors; 1.5 Providing Energy; 1.5.1 Direct Nucleotide Regeneration; 1.5.2 Indirect Nucleotide Regeneration 1.6 Enhancing Protein Folding1.6.1 Temperature Effects; 1.6.2 Cell Extract Concentration; 1.6.3 Effects of Folding Ligands; 1.6.4 Effects of Chaperones and Foldases; 1.6.5 Effects of Detergents; 2 The Constructive Approach for Cell-free Translation; 2.1 Introduction; 2.2 The Process of Protein Synthesis; 2.2.1 Polypeptide Synthesis; 2.2.2 Protein Maturation; 2.3 A Constructive Approach to Protein Synthesis; 2.3.1 In Vitro Reconstitution of Polypeptide Synthesis; 2.3.2 Protocol of Protein Synthesis using PURE System; 2.3.3 Addition of Protein Folding Machinery to the PURE System 2.3.4 Integration of a Membrane Targeting System with the PURE system2.3.5 Protein Synthesis using the PURE System containing Molecular Chaperones; 2.4 Conclusion; 3 Functional Genomic Analysis using Sequential Cell-free Protein Synthesis; 3.1 Introduction; 3.1.1 The Post-genomic Era; 3.1.2 Cell-free Protein Synthesis (CFPS) as a Functional Proteomic Tool; 3.2 Developing an enabling Technology for Sequential Expression Analysis; 3.2.1 Improving Linear Template Stability; 3.2.2 Improving PCR Reactions for generating Genomic Linear Templates 3.2.3 Optimizing Cofactor Concentrations for Enzyme Activation3.3 Demonstrating Functional Genomic Analysis with CFPS; 3.3.1 Isolation and Expression of Genomic Targets; 3.3.2 Effects of Sample Library on β-Lactamase Expression and Activity; 3.4 Conclusions and Projections; 4 Cell-free Technology for Rapid Production of Patient-specific Fusion Protein Vaccines; 4.1 Introduction; 4.1.1 Lymphoma and Fusion Protein Vaccine Treatments; 4.1.2 Comparing Cell-free and In Vivo Production Systems; 4.2 Developing the Fusion Protein Construct and the Cell-free Production Process 4.2.1 Fusion-protein Production in the Cell-free System |
| Record Nr. | UNINA-9910876999303321 |
| Weinheim, : Wiley-VCH, c2008 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Chemical ligation : tools for biomolecule synthesis and modification / / edited by Luca D. D'Andrea and Alessandra Romanelli
| Chemical ligation : tools for biomolecule synthesis and modification / / edited by Luca D. D'Andrea and Alessandra Romanelli |
| Pubbl/distr/stampa | Hoboken, New Jersey : , : Wiley, , 2017 |
| Descrizione fisica | 1 online resource (583 pages) : illustrations (some color) |
| Disciplina | 612/.015756 |
| Soggetto topico |
Biosynthesis
Peptides - Synthesis Proteins - Synthesis Bioorganic chemistry Chemistry, Organic |
| ISBN |
1-119-04413-8
1-119-04409-X 1-119-04411-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910271052903321 |
| Hoboken, New Jersey : , : Wiley, , 2017 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Chemical ligation : tools for biomolecule synthesis and modification / / edited by Luca D. D'Andrea and Alessandra Romanelli
| Chemical ligation : tools for biomolecule synthesis and modification / / edited by Luca D. D'Andrea and Alessandra Romanelli |
| Pubbl/distr/stampa | Hoboken, New Jersey : , : Wiley, , 2017 |
| Descrizione fisica | 1 online resource (583 pages) : illustrations (some color) |
| Disciplina | 612/.015756 |
| Soggetto topico |
Biosynthesis
Peptides - Synthesis Proteins - Synthesis Bioorganic chemistry Chemistry, Organic |
| ISBN |
1-119-04413-8
1-119-04409-X 1-119-04411-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910807725603321 |
| Hoboken, New Jersey : , : Wiley, , 2017 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Homocysteine in protein structure/function and human disease : chemical biology of homocysteine-containing proteins / / Hieronim Jakubowski
| Homocysteine in protein structure/function and human disease : chemical biology of homocysteine-containing proteins / / Hieronim Jakubowski |
| Autore | Jakubowski Hieronim |
| Edizione | [1st ed. 2013.] |
| Pubbl/distr/stampa | Wien, : Springer-Verlag, 2013 |
| Descrizione fisica | 1 online resource (174 p.) |
| Disciplina | 616.1071 |
| Soggetto topico |
Proteins - Synthesis
Homocysteine |
| ISBN | 3-7091-1410-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | 1. Introduction -- 2. Homocysteine. Chemical Synthesis -- Physicochemical Properties -- Quantification Methods -- Metabolic Pathways -- Clinical significance -- 3. Homocysteine Thiolactone -- Chemical Synthesis -- Physicochemical Properties -- Quantification Methods -- Biological Formation and Turnover -- Clinical Significance -- 4. N-Homocysteinylated Proteins -- Functional Consequences -- Albumin -- Fibrinogen -- Cytochrome c -- Other Proteins -- Quantification Methods -- Total N-homocysteinylation assays -- Site-specific N-homocysteinylation assays -- Formation in vivo -- Turnover to Nε-Homocysteinyl-lysine -- Biological Consequences and Clinical Significance -- 5. S-Homocysteinylated Proteins -- Formation in vitro -- Functional Consequences -- Annexin -- Metallothionein -- Fibrilin, Fibronectin, Tropoelastin -- Detection in vivo -- Biological consequences -- Conclusions -- References. |
| Record Nr. | UNINA-9910437613703321 |
Jakubowski Hieronim
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| Wien, : Springer-Verlag, 2013 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Methods in protein biochemistry [[electronic resource] /] / edited by Harald Tschesche
| Methods in protein biochemistry [[electronic resource] /] / edited by Harald Tschesche |
| Pubbl/distr/stampa | Berlin, : De Gruyter, c2012 |
| Descrizione fisica | 1 online resource (378 p.) |
| Disciplina | 572/.67 |
| Altri autori (Persone) | TschescheHarald |
| Soggetto topico |
Proteins - Analysis
Proteins - Synthesis Proteomics |
| Soggetto genere / forma | Electronic books. |
| ISBN |
3-11-048150-2
3-11-025236-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Three-phase partitioning -- Folding and degradation functions of molecular chaperones -- Membrane protein folding in detergents -- Glycoprotein folding quality control in the endoplasmic reticulum -- Protein import into the intermembrane space of mitochondria -- On-membrane identification of gel-resolved proteins by MALDI-MS -- Analysis of protein complexes using chemical cross-linking and mass spectrometry -- Single-crystal spectroscopy correlated with X-ray crystallography provides complementary perspectives on macromolecular function -- Wide-angle X-ray solution scattering (WAXS) -- Where purity matters : recombinant versus synthetic peptides in beta amyloid formation -- Chemical modification of proteins in living cells -- Proteomics of human bronchoalveolar lavage fluid : discovery of biomarkers of chronic obstructive pulmonary disease with difference gel electrophoresis (DIGE) and mass spectrometry -- Proteomic of Duchenne muscular dystrophy -- Target-oriented peptide arrays in a palliative approach to cystic fibrosis -- Probing protein dynamics in vivo using backbone cyclization : bacterial acyl carrier protein as a case study -- The protein epitope mimetic approach to protein-protein interaction inhibitors -- The structural biology of alpha-1 antitrypsin deficiency and the serpinopathies. |
| Record Nr. | UNINA-9910465529303321 |
| Berlin, : De Gruyter, c2012 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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