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Biological activities of ribosome-inactivating proteins / / Lucía Citores, José M. Ferreras, editors
Biological activities of ribosome-inactivating proteins / / Lucía Citores, José M. Ferreras, editors
Pubbl/distr/stampa Basel : , : MDPI - Multidisciplinary Digital Publishing Institute, , [2023]
Descrizione fisica 1 online resource (196 pages)
Disciplina 574.8734
Soggetto topico Ribosomes - Research
Proteins - Synthesis
ISBN 3-0365-6823-9
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910683381003321
Basel : , : MDPI - Multidisciplinary Digital Publishing Institute, , [2023]
Materiale a stampa
Lo trovi qui: Univ. Federico II
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Biophysical Chemistry of Proteins : An Introduction to Laboratory Methods / / by Engelbert Buxbaum
Biophysical Chemistry of Proteins : An Introduction to Laboratory Methods / / by Engelbert Buxbaum
Autore Buxbaum Engelbert
Edizione [2nd ed. 2025.]
Pubbl/distr/stampa New York, NY : , : Springer US : , : Imprint : Springer, , 2025
Descrizione fisica 1 online resource (911 pages)
Disciplina 572.6
Soggetto topico Proteins
Proteins - Synthesis
Biochemistry
Chemistry
Protein Biochemistry
Protein Synthesis and Translation
ISBN 1-0716-4124-7
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Analytical techniques -- Microscopy -- Single molecule techniques -- Preparation of cells and tissues for microscopy -- Principles of optical spectroscopy -- Photometry -- Fluorimetry -- Chemiluminescence -- Electrophoresis -- Immunological methods -- Isotope techniques -- Purification of proteins -- Homogenisation and fractionisation of cells and tissues -- Isolation of organelles -- Precipitation methods -- Chromatography -- Membrane proteins -- Determination of protein concentration -- Cell culture -- Protein modification and inactivation -- General technical remarks -- Amine-reactive reagents -- Thiol- and disulphide reactive reagents -- Reagents for other groups -- Cross-linkers -- Detection methods -- Spontaneous reactions in proteins -- Protein size and shape -- Centrifugation -- Osmotic pressure -- Diffusion -- Viscosity -- Non-resonant interactions with electromagnetic waves -- Protein structure -- Protein sequencing -- Synthesis of peptides -- Protein secondary structure -- Structure of protein-ligand complexes -- 3D-structures -- Folding and unfolding of proteins -- Enzyme kinetics -- Steady-state kinetics -- Leaving the steady state: Analysis of progress curves -- Reaction velocities -- Isotope effects -- Isotope exchange -- Protein-ligand interactions -- General conditions for interpretable results -- Binding equations -- Methods to measure binding equilibria -- Temperature effects on binding equilibrium and reaction rate -- Industrial enzymology -- Industrial enzyme use -- Immobilised enzymes -- Special statistics -- Quality control -- Testing whether or not a model fits the data -- Appendix -- List of symbols -- Greek alphabet -- Properties of electrophoretic buffers -- Bond properties -- Acronyms.
Record Nr. UNINA-9911015636003321
Buxbaum Engelbert  
New York, NY : , : Springer US : , : Imprint : Springer, , 2025
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Biophysics for therapeutic protein development / / Linda O. Narhi, editor
Biophysics for therapeutic protein development / / Linda O. Narhi, editor
Edizione [1st ed. 2013.]
Pubbl/distr/stampa New York, : Springer, 2013
Descrizione fisica 1 online resource (297 p.)
Disciplina 615.58
Altri autori (Persone) NarhiLinda O
Collana Biophysics for the life sciences
Soggetto topico Proteins - Biotechnology
Cell culture
Proteins - Synthesis
Proteins - Therapeutic use
Biophysics
ISBN 1-299-40831-1
1-4614-4316-4
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Introduction -- High Throughput Biophysical Approaches to Therapeutic Protein Development -- Techniques for Higher Order Structure Determination -- Biophysical Techniques for Protein Size Distribution Analysis -- Qualification of biophysical methods for the analysis of protein therapeutics -- Application of Biophysics to the Early Developability Assessment of Therapeutic Candidates and its Application to Enhance Developability Properties -- Application of Biophysics in Formulation, Process and Product Characterization: Selected Case Studies -- Biophysical Analysis in Support of Development of Protein Pharmaceuticals -- Case Studies Applying Biophysical Techniques to Better Characterize Protein Aggregates and Particulates of Varying Size -- Investigation of Non Conformance During Protein Therapeutic Manufacturing -- Higher order structure and protein aggregate characterization of protein therapeutics: perspectives from good manufacturing practices and regulatory guidance -- Index.
Record Nr. UNINA-9910437842603321
New York, : Springer, 2013
Materiale a stampa
Lo trovi qui: Univ. Federico II
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Cell-Free Protein Synthesis / / edited by Manish Biyani
Cell-Free Protein Synthesis / / edited by Manish Biyani
Pubbl/distr/stampa Rijeka : , : InTech, , 2012
Descrizione fisica 1 online resource (xi, 146 pages) : illustrations
Disciplina 574.19296
Soggetto topico Proteins - Synthesis
ISBN 953-51-5328-5
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910138450503321
Rijeka : , : InTech, , 2012
Materiale a stampa
Lo trovi qui: Univ. Federico II
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Cell-free protein synthesis [[electronic resource] ] : methods and protocols / / edited by Alexander S. Spirin and James R. Swartz
Cell-free protein synthesis [[electronic resource] ] : methods and protocols / / edited by Alexander S. Spirin and James R. Swartz
Pubbl/distr/stampa Weinheim, : Wiley-VCH, c2008
Descrizione fisica 1 online resource (264 p.)
Disciplina 572.6
Altri autori (Persone) SpirinA. S (Aleksandr Sergeevich)
SwartzJames R
Soggetto topico Proteins - Synthesis
Genetic translation
Soggetto genere / forma Electronic books.
ISBN 3-527-69150-2
1-281-94699-0
9786611946999
3-527-62270-5
3-527-62269-1
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Cell-free Protein Synthesis; Contents; Preface; List of Contributors; 1 Cell-free Protein Synthesis Systems: Historical Landmarks, Classification, and General Methods; 1.1 Introduction: Historical Landmarks; 1.1.1 Discovery of Protein Synthesis in Cell Extracts; 1.1.2 Translation of Exogenous Messages; 1.1.3 Coupled Transcription-translation in Bacterial Extracts; 1.1.4 Combined Transcription-translation Systems; 1.1.5 Continuous Flow/Continuous Exchange Principle; 1.2 Prokaryotic and Eukaryotic Types of Cell-free Expression Systems; 1.2.1 Cell Extracts; 1.2.1.1 E. coli extract (ECE)
1.2.1.2 Wheat Germ Extract (WGE)1.2.1.3 Rabbit Reticulocyte Lysate (RRL); 1.2.2 Genetic Constructs (Expression Vectors); 1.2.2.1 Prokaryotic Systems; 1.2.2.2 Eukaryotic Systems; 1.3 Preparing Cell Extracts; 1.3.1 E. coli Extracts; 1.3.1.1 Genetics; 1.3.1.2 Cell Growth; 1.3.1.3 Extract Preparation; 1.3.2 Wheat Germ Extracts; 1.4 Designing Reaction Composition; 1.4.1 Mg(2+) and Phosphate; 1.4.2 Other Salts; 1.4.3 Nucleotides and Amino Acids; 1.4.4 Stabilization Reagents; 1.4.5 Other Factors; 1.5 Providing Energy; 1.5.1 Direct Nucleotide Regeneration; 1.5.2 Indirect Nucleotide Regeneration
1.6 Enhancing Protein Folding1.6.1 Temperature Effects; 1.6.2 Cell Extract Concentration; 1.6.3 Effects of Folding Ligands; 1.6.4 Effects of Chaperones and Foldases; 1.6.5 Effects of Detergents; 2 The Constructive Approach for Cell-free Translation; 2.1 Introduction; 2.2 The Process of Protein Synthesis; 2.2.1 Polypeptide Synthesis; 2.2.2 Protein Maturation; 2.3 A Constructive Approach to Protein Synthesis; 2.3.1 In Vitro Reconstitution of Polypeptide Synthesis; 2.3.2 Protocol of Protein Synthesis using PURE System; 2.3.3 Addition of Protein Folding Machinery to the PURE System
2.3.4 Integration of a Membrane Targeting System with the PURE system2.3.5 Protein Synthesis using the PURE System containing Molecular Chaperones; 2.4 Conclusion; 3 Functional Genomic Analysis using Sequential Cell-free Protein Synthesis; 3.1 Introduction; 3.1.1 The Post-genomic Era; 3.1.2 Cell-free Protein Synthesis (CFPS) as a Functional Proteomic Tool; 3.2 Developing an enabling Technology for Sequential Expression Analysis; 3.2.1 Improving Linear Template Stability; 3.2.2 Improving PCR Reactions for generating Genomic Linear Templates
3.2.3 Optimizing Cofactor Concentrations for Enzyme Activation3.3 Demonstrating Functional Genomic Analysis with CFPS; 3.3.1 Isolation and Expression of Genomic Targets; 3.3.2 Effects of Sample Library on β-Lactamase Expression and Activity; 3.4 Conclusions and Projections; 4 Cell-free Technology for Rapid Production of Patient-specific Fusion Protein Vaccines; 4.1 Introduction; 4.1.1 Lymphoma and Fusion Protein Vaccine Treatments; 4.1.2 Comparing Cell-free and In Vivo Production Systems; 4.2 Developing the Fusion Protein Construct and the Cell-free Production Process
4.2.1 Fusion-protein Production in the Cell-free System
Record Nr. UNINA-9910144101803321
Weinheim, : Wiley-VCH, c2008
Materiale a stampa
Lo trovi qui: Univ. Federico II
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Cell-free protein synthesis [[electronic resource] ] : methods and protocols / / edited by Alexander S. Spirin and James R. Swartz
Cell-free protein synthesis [[electronic resource] ] : methods and protocols / / edited by Alexander S. Spirin and James R. Swartz
Pubbl/distr/stampa Weinheim, : Wiley-VCH, c2008
Descrizione fisica 1 online resource (264 p.)
Disciplina 572.6
Altri autori (Persone) SpirinA. S (Aleksandr Sergeevich)
SwartzJames R
Soggetto topico Proteins - Synthesis
Genetic translation
ISBN 3-527-69150-2
1-281-94699-0
9786611946999
3-527-62270-5
3-527-62269-1
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Cell-free Protein Synthesis; Contents; Preface; List of Contributors; 1 Cell-free Protein Synthesis Systems: Historical Landmarks, Classification, and General Methods; 1.1 Introduction: Historical Landmarks; 1.1.1 Discovery of Protein Synthesis in Cell Extracts; 1.1.2 Translation of Exogenous Messages; 1.1.3 Coupled Transcription-translation in Bacterial Extracts; 1.1.4 Combined Transcription-translation Systems; 1.1.5 Continuous Flow/Continuous Exchange Principle; 1.2 Prokaryotic and Eukaryotic Types of Cell-free Expression Systems; 1.2.1 Cell Extracts; 1.2.1.1 E. coli extract (ECE)
1.2.1.2 Wheat Germ Extract (WGE)1.2.1.3 Rabbit Reticulocyte Lysate (RRL); 1.2.2 Genetic Constructs (Expression Vectors); 1.2.2.1 Prokaryotic Systems; 1.2.2.2 Eukaryotic Systems; 1.3 Preparing Cell Extracts; 1.3.1 E. coli Extracts; 1.3.1.1 Genetics; 1.3.1.2 Cell Growth; 1.3.1.3 Extract Preparation; 1.3.2 Wheat Germ Extracts; 1.4 Designing Reaction Composition; 1.4.1 Mg(2+) and Phosphate; 1.4.2 Other Salts; 1.4.3 Nucleotides and Amino Acids; 1.4.4 Stabilization Reagents; 1.4.5 Other Factors; 1.5 Providing Energy; 1.5.1 Direct Nucleotide Regeneration; 1.5.2 Indirect Nucleotide Regeneration
1.6 Enhancing Protein Folding1.6.1 Temperature Effects; 1.6.2 Cell Extract Concentration; 1.6.3 Effects of Folding Ligands; 1.6.4 Effects of Chaperones and Foldases; 1.6.5 Effects of Detergents; 2 The Constructive Approach for Cell-free Translation; 2.1 Introduction; 2.2 The Process of Protein Synthesis; 2.2.1 Polypeptide Synthesis; 2.2.2 Protein Maturation; 2.3 A Constructive Approach to Protein Synthesis; 2.3.1 In Vitro Reconstitution of Polypeptide Synthesis; 2.3.2 Protocol of Protein Synthesis using PURE System; 2.3.3 Addition of Protein Folding Machinery to the PURE System
2.3.4 Integration of a Membrane Targeting System with the PURE system2.3.5 Protein Synthesis using the PURE System containing Molecular Chaperones; 2.4 Conclusion; 3 Functional Genomic Analysis using Sequential Cell-free Protein Synthesis; 3.1 Introduction; 3.1.1 The Post-genomic Era; 3.1.2 Cell-free Protein Synthesis (CFPS) as a Functional Proteomic Tool; 3.2 Developing an enabling Technology for Sequential Expression Analysis; 3.2.1 Improving Linear Template Stability; 3.2.2 Improving PCR Reactions for generating Genomic Linear Templates
3.2.3 Optimizing Cofactor Concentrations for Enzyme Activation3.3 Demonstrating Functional Genomic Analysis with CFPS; 3.3.1 Isolation and Expression of Genomic Targets; 3.3.2 Effects of Sample Library on β-Lactamase Expression and Activity; 3.4 Conclusions and Projections; 4 Cell-free Technology for Rapid Production of Patient-specific Fusion Protein Vaccines; 4.1 Introduction; 4.1.1 Lymphoma and Fusion Protein Vaccine Treatments; 4.1.2 Comparing Cell-free and In Vivo Production Systems; 4.2 Developing the Fusion Protein Construct and the Cell-free Production Process
4.2.1 Fusion-protein Production in the Cell-free System
Record Nr. UNINA-9910829884203321
Weinheim, : Wiley-VCH, c2008
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Cell-free protein synthesis : methods and protocols / / edited by Alexander S. Spirin and James R. Swartz
Cell-free protein synthesis : methods and protocols / / edited by Alexander S. Spirin and James R. Swartz
Pubbl/distr/stampa Weinheim, : Wiley-VCH, c2008
Descrizione fisica 1 online resource (264 p.)
Disciplina 572.6
Altri autori (Persone) SpirinA. S (Aleksandr Sergeevich)
SwartzJames R
Soggetto topico Proteins - Synthesis
Genetic translation
ISBN 9786611946999
9783527691500
3527691502
9781281946997
1281946990
9783527622702
3527622705
9783527622696
3527622691
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Cell-free Protein Synthesis; Contents; Preface; List of Contributors; 1 Cell-free Protein Synthesis Systems: Historical Landmarks, Classification, and General Methods; 1.1 Introduction: Historical Landmarks; 1.1.1 Discovery of Protein Synthesis in Cell Extracts; 1.1.2 Translation of Exogenous Messages; 1.1.3 Coupled Transcription-translation in Bacterial Extracts; 1.1.4 Combined Transcription-translation Systems; 1.1.5 Continuous Flow/Continuous Exchange Principle; 1.2 Prokaryotic and Eukaryotic Types of Cell-free Expression Systems; 1.2.1 Cell Extracts; 1.2.1.1 E. coli extract (ECE)
1.2.1.2 Wheat Germ Extract (WGE)1.2.1.3 Rabbit Reticulocyte Lysate (RRL); 1.2.2 Genetic Constructs (Expression Vectors); 1.2.2.1 Prokaryotic Systems; 1.2.2.2 Eukaryotic Systems; 1.3 Preparing Cell Extracts; 1.3.1 E. coli Extracts; 1.3.1.1 Genetics; 1.3.1.2 Cell Growth; 1.3.1.3 Extract Preparation; 1.3.2 Wheat Germ Extracts; 1.4 Designing Reaction Composition; 1.4.1 Mg(2+) and Phosphate; 1.4.2 Other Salts; 1.4.3 Nucleotides and Amino Acids; 1.4.4 Stabilization Reagents; 1.4.5 Other Factors; 1.5 Providing Energy; 1.5.1 Direct Nucleotide Regeneration; 1.5.2 Indirect Nucleotide Regeneration
1.6 Enhancing Protein Folding1.6.1 Temperature Effects; 1.6.2 Cell Extract Concentration; 1.6.3 Effects of Folding Ligands; 1.6.4 Effects of Chaperones and Foldases; 1.6.5 Effects of Detergents; 2 The Constructive Approach for Cell-free Translation; 2.1 Introduction; 2.2 The Process of Protein Synthesis; 2.2.1 Polypeptide Synthesis; 2.2.2 Protein Maturation; 2.3 A Constructive Approach to Protein Synthesis; 2.3.1 In Vitro Reconstitution of Polypeptide Synthesis; 2.3.2 Protocol of Protein Synthesis using PURE System; 2.3.3 Addition of Protein Folding Machinery to the PURE System
2.3.4 Integration of a Membrane Targeting System with the PURE system2.3.5 Protein Synthesis using the PURE System containing Molecular Chaperones; 2.4 Conclusion; 3 Functional Genomic Analysis using Sequential Cell-free Protein Synthesis; 3.1 Introduction; 3.1.1 The Post-genomic Era; 3.1.2 Cell-free Protein Synthesis (CFPS) as a Functional Proteomic Tool; 3.2 Developing an enabling Technology for Sequential Expression Analysis; 3.2.1 Improving Linear Template Stability; 3.2.2 Improving PCR Reactions for generating Genomic Linear Templates
3.2.3 Optimizing Cofactor Concentrations for Enzyme Activation3.3 Demonstrating Functional Genomic Analysis with CFPS; 3.3.1 Isolation and Expression of Genomic Targets; 3.3.2 Effects of Sample Library on β-Lactamase Expression and Activity; 3.4 Conclusions and Projections; 4 Cell-free Technology for Rapid Production of Patient-specific Fusion Protein Vaccines; 4.1 Introduction; 4.1.1 Lymphoma and Fusion Protein Vaccine Treatments; 4.1.2 Comparing Cell-free and In Vivo Production Systems; 4.2 Developing the Fusion Protein Construct and the Cell-free Production Process
4.2.1 Fusion-protein Production in the Cell-free System
Record Nr. UNINA-9911019098903321
Weinheim, : Wiley-VCH, c2008
Materiale a stampa
Lo trovi qui: Univ. Federico II
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Chemical ligation : tools for biomolecule synthesis and modification / / edited by Luca D. D'Andrea and Alessandra Romanelli
Chemical ligation : tools for biomolecule synthesis and modification / / edited by Luca D. D'Andrea and Alessandra Romanelli
Pubbl/distr/stampa Hoboken, New Jersey : , : Wiley, , 2017
Descrizione fisica 1 online resource (583 pages) : illustrations (some color)
Disciplina 612/.015756
Soggetto topico Biosynthesis
Peptides - Synthesis
Proteins - Synthesis
Bioorganic chemistry
Chemistry, Organic
ISBN 1-119-04413-8
1-119-04409-X
1-119-04411-1
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910271052903321
Hoboken, New Jersey : , : Wiley, , 2017
Materiale a stampa
Lo trovi qui: Univ. Federico II
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Chemical ligation : tools for biomolecule synthesis and modification / / edited by Luca D. D'Andrea and Alessandra Romanelli
Chemical ligation : tools for biomolecule synthesis and modification / / edited by Luca D. D'Andrea and Alessandra Romanelli
Pubbl/distr/stampa Hoboken, New Jersey : , : Wiley, , 2017
Descrizione fisica 1 online resource (583 pages) : illustrations (some color)
Disciplina 612/.015756
Soggetto topico Biosynthesis
Peptides - Synthesis
Proteins - Synthesis
Bioorganic chemistry
Chemistry, Organic
ISBN 1-119-04413-8
1-119-04409-X
1-119-04411-1
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910807725603321
Hoboken, New Jersey : , : Wiley, , 2017
Materiale a stampa
Lo trovi qui: Univ. Federico II
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Histone and Non-Histone Reversible Acetylation in Development, Aging and Disease / / edited by Marta Halasa, Anna Wawruszak
Histone and Non-Histone Reversible Acetylation in Development, Aging and Disease / / edited by Marta Halasa, Anna Wawruszak
Autore Halasa Marta
Edizione [1st ed. 2025.]
Pubbl/distr/stampa Cham : , : Springer Nature Switzerland : , : Imprint : Springer, , 2025
Descrizione fisica 1 online resource (646 pages)
Disciplina 572.645
Altri autori (Persone) WawruszakAnna
Collana Results and Problems in Cell Differentiation
Soggetto topico Post-translational modification
Epigenetics
Proteins
Cell differentiation
Proteins - Synthesis
Tumor suppressor proteins
Antioncogenes
Post-translational Modifications
Protein Biochemistry
Cell Differentiation
Protein Synthesis and Translation
Tumour-suppressor Proteins
ISBN 3-031-91459-7
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Part I. Introduction to Reversible Acetylation and Its Biological Consequences -- Chapter 1. Histone and Non-Histone Reversible Acetylation in Development, Aging and Disease -- Chapter 2. Acetylation-Mediated Epigenetic Consequences for Biological Control and Cancer -- Part II. Acetylation of Cytoskeleton-Associated Proteins -- Chapter 3. Acetylation and Deacetylation of Cytoskeleton-Associated Proteins -- Chapter 4. Tubulin Acetylation: A Critical Regulator of Microtubule Function -- Chapter 5. Tubulin Acetylation and the Cellular Mechanosensing and Stress Response -- Part III. Acetylation in Development -- Chapter 6. Reversible Histone Acetylation During Preimplantation Embryo Development in Mammals -- Chapter 7. Epigenetic Echoes: Decoding the Acetylation Journey from Neural Crest to Melanocyte -- Part IV. Acetylation in Physiological and Pathological Processes -- Chapter 8. Impact of Histone Acetyltransferases and His-tone Deacetylases on Adult Brain Myelin Plastici-ty -- Chapter 9. Acetylation in Cardiac Aging: Molecular Mechanism and Therapeutic Approaches -- Chapter 10. Regulatory Roles for SIRT1 in Aging and Immunosenescence -- Chapter 11. Repurposing Histone Deacetylase Inhibitors for Management of Solid Organ Transplant Rejection -- Chapter 12. Acetylation in Viral Infection and Disease -- Chapter 13. Reversible Acetylation of Non-Histone Proteins in Human Cancers -- Chapter 14. Lysine Acetyltransferase 6 Complexes in Neurodevelopmental Disorders and Different Types of Cancer -- Chapter 15. Bromodomain and PHD Finger-Containing Protein 1: From Biological Functions to a Developmental Disorder, Cancer and Therapeutics.
Record Nr. UNINA-9911015876103321
Halasa Marta  
Cham : , : Springer Nature Switzerland : , : Imprint : Springer, , 2025
Materiale a stampa
Lo trovi qui: Univ. Federico II
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