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Biophysical Chemistry of Proteins : An Introduction to Laboratory Methods / / by Engelbert Buxbaum
| Biophysical Chemistry of Proteins : An Introduction to Laboratory Methods / / by Engelbert Buxbaum |
| Autore | Buxbaum Engelbert |
| Edizione | [2nd ed. 2025.] |
| Pubbl/distr/stampa | New York, NY : , : Springer US : , : Imprint : Springer, , 2025 |
| Descrizione fisica | 1 online resource (911 pages) |
| Disciplina | 572.6 |
| Soggetto topico |
Proteins
Proteins - Synthesis Biochemistry Chemistry Protein Biochemistry Protein Synthesis and Translation |
| ISBN | 1-0716-4124-7 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Analytical techniques -- Microscopy -- Single molecule techniques -- Preparation of cells and tissues for microscopy -- Principles of optical spectroscopy -- Photometry -- Fluorimetry -- Chemiluminescence -- Electrophoresis -- Immunological methods -- Isotope techniques -- Purification of proteins -- Homogenisation and fractionisation of cells and tissues -- Isolation of organelles -- Precipitation methods -- Chromatography -- Membrane proteins -- Determination of protein concentration -- Cell culture -- Protein modification and inactivation -- General technical remarks -- Amine-reactive reagents -- Thiol- and disulphide reactive reagents -- Reagents for other groups -- Cross-linkers -- Detection methods -- Spontaneous reactions in proteins -- Protein size and shape -- Centrifugation -- Osmotic pressure -- Diffusion -- Viscosity -- Non-resonant interactions with electromagnetic waves -- Protein structure -- Protein sequencing -- Synthesis of peptides -- Protein secondary structure -- Structure of protein-ligand complexes -- 3D-structures -- Folding and unfolding of proteins -- Enzyme kinetics -- Steady-state kinetics -- Leaving the steady state: Analysis of progress curves -- Reaction velocities -- Isotope effects -- Isotope exchange -- Protein-ligand interactions -- General conditions for interpretable results -- Binding equations -- Methods to measure binding equilibria -- Temperature effects on binding equilibrium and reaction rate -- Industrial enzymology -- Industrial enzyme use -- Immobilised enzymes -- Special statistics -- Quality control -- Testing whether or not a model fits the data -- Appendix -- List of symbols -- Greek alphabet -- Properties of electrophoretic buffers -- Bond properties -- Acronyms. |
| Record Nr. | UNINA-9911015636003321 |
Buxbaum Engelbert
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| New York, NY : , : Springer US : , : Imprint : Springer, , 2025 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Histone and Non-Histone Reversible Acetylation in Development, Aging and Disease / / edited by Marta Halasa, Anna Wawruszak
| Histone and Non-Histone Reversible Acetylation in Development, Aging and Disease / / edited by Marta Halasa, Anna Wawruszak |
| Autore | Halasa Marta |
| Edizione | [1st ed. 2025.] |
| Pubbl/distr/stampa | Cham : , : Springer Nature Switzerland : , : Imprint : Springer, , 2025 |
| Descrizione fisica | 1 online resource (646 pages) |
| Disciplina | 572.645 |
| Altri autori (Persone) | WawruszakAnna |
| Collana | Results and Problems in Cell Differentiation |
| Soggetto topico |
Post-translational modification
Epigenetics Proteins Cell differentiation Proteins - Synthesis Tumor suppressor proteins Antioncogenes Post-translational Modifications Protein Biochemistry Cell Differentiation Protein Synthesis and Translation Tumour-suppressor Proteins |
| ISBN | 3-031-91459-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Part I. Introduction to Reversible Acetylation and Its Biological Consequences -- Chapter 1. Histone and Non-Histone Reversible Acetylation in Development, Aging and Disease -- Chapter 2. Acetylation-Mediated Epigenetic Consequences for Biological Control and Cancer -- Part II. Acetylation of Cytoskeleton-Associated Proteins -- Chapter 3. Acetylation and Deacetylation of Cytoskeleton-Associated Proteins -- Chapter 4. Tubulin Acetylation: A Critical Regulator of Microtubule Function -- Chapter 5. Tubulin Acetylation and the Cellular Mechanosensing and Stress Response -- Part III. Acetylation in Development -- Chapter 6. Reversible Histone Acetylation During Preimplantation Embryo Development in Mammals -- Chapter 7. Epigenetic Echoes: Decoding the Acetylation Journey from Neural Crest to Melanocyte -- Part IV. Acetylation in Physiological and Pathological Processes -- Chapter 8. Impact of Histone Acetyltransferases and His-tone Deacetylases on Adult Brain Myelin Plastici-ty -- Chapter 9. Acetylation in Cardiac Aging: Molecular Mechanism and Therapeutic Approaches -- Chapter 10. Regulatory Roles for SIRT1 in Aging and Immunosenescence -- Chapter 11. Repurposing Histone Deacetylase Inhibitors for Management of Solid Organ Transplant Rejection -- Chapter 12. Acetylation in Viral Infection and Disease -- Chapter 13. Reversible Acetylation of Non-Histone Proteins in Human Cancers -- Chapter 14. Lysine Acetyltransferase 6 Complexes in Neurodevelopmental Disorders and Different Types of Cancer -- Chapter 15. Bromodomain and PHD Finger-Containing Protein 1: From Biological Functions to a Developmental Disorder, Cancer and Therapeutics. |
| Record Nr. | UNINA-9911015876103321 |
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Halasa Marta
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| Cham : , : Springer Nature Switzerland : , : Imprint : Springer, , 2025 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Peptide Therapeutics : Fundamentals of Design, Development, and Delivery / / edited by Seetharama D. Jois
| Peptide Therapeutics : Fundamentals of Design, Development, and Delivery / / edited by Seetharama D. Jois |
| Edizione | [1st ed. 2022.] |
| Pubbl/distr/stampa | Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022 |
| Descrizione fisica | 1 online resource (321 pages) |
| Disciplina | 615.19 |
| Collana | AAPS Advances in the Pharmaceutical Sciences Series |
| Soggetto topico |
Pharmaceutical chemistry
Drug delivery systems Drugs - Design Pharmacology Pharmacy Proteins - Synthesis Pharmaceutics Drug Delivery Structure-Based Drug Design Protein Synthesis and Translation Medicaments peptídics Química farmacèutica |
| Soggetto genere / forma | Llibres electrònics |
| ISBN |
9783031045448
9783031045431 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Basic Concepts of Design of Peptide based Therapeutics -- Computational Methods in Peptide Based Drug Design -- Synthesis, Purification, and Characterization of Linear and Cyclic Peptides -- Large Scale Synthesis of Peptides, Manufacturing Peptide Therapeutics -- Stability of Peptides -- Peptide Delivery Systems -- Liposomes and Nanoparticle Formulations for Peptide Delivery -- Delivery of Peptides Across the Blood-brain Barrier (BBB) -- Peptide Therapeutics Regulatory Considerations. |
| Record Nr. | UNINA-9910616399103321 |
| Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Protein Folding Dynamics and Stability : Experimental and Computational Methods / / edited by Prakash Saudagar, Timir Tripathi
| Protein Folding Dynamics and Stability : Experimental and Computational Methods / / edited by Prakash Saudagar, Timir Tripathi |
| Autore | Saudagar Prakash |
| Edizione | [1st ed. 2023.] |
| Pubbl/distr/stampa | Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2023 |
| Descrizione fisica | 1 online resource (287 pages) |
| Disciplina | 572.633 |
| Altri autori (Persone) | TripathiTimir |
| Soggetto topico |
Proteins
Molecular biology Biology Proteins - Synthesis Protein Biochemistry Molecular Biology Biological Sciences Protein Synthesis and Translation |
| ISBN | 981-9920-79-5 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Chapter 1. Applications of Circular Dichroism Spectroscopy in Studying Protein Folding, Stability, and Interaction -- Chapter 2. Fluorescence Spectroscopy-based Methods to Study Protein Folding Dynamics -- Chapter 3. Applications of Differential Scanning Calorimetry in Studying Folding and Stability of Proteins -- Chapter 4. Nuclear Magnetic Resonance Spectroscopy to Analyze Protein Folding and Dynamics -- Chapter 5. Molecular Dynamics Simulation Methods to Study Protein Structural Dynamics. Chapter 6. Molecular Dynamics Simulation to Study Protein Conformation and Ligand Interaction -- Chapter 7. Monte Carlo Approaches to Study Protein Conformation Ensembles. Chapter 8. Markov State Models of Molecular Simulations to Study Protein Folding and Dynamics -- Chapter 9. Enhanced Sampling and Free Energy Methods to Study Protein Folding and Dynamics -- Chapter 10. Investigating Protein Unfolding and Stability using Chaotropic Agents and Molecular Dynamics Simulation -- Chapter 11. pH-basedMolecular Dynamics Simulation for Analyzing Protein Structure and Folding -- Chapter 12. Molecular Dynamics Simulation to Study Thermal Unfolding in Proteins -- Chapter 13. Principles, Methods, and Applications of Protein Folding Inside Cells. . |
| Record Nr. | UNINA-9910728396303321 |
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Saudagar Prakash
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| Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2023 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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