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BSL3 and BSL4 agents [[electronic resource] ] : epidemiology, microbiology, and practical guidelines / / edited by Mandy C. Elschner ... [et al.]
| BSL3 and BSL4 agents [[electronic resource] ] : epidemiology, microbiology, and practical guidelines / / edited by Mandy C. Elschner ... [et al.] |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | Weinheim, Germany, : Wiley-Blackwell, c2012 |
| Descrizione fisica | 1 online resource (401 p.) |
| Disciplina |
579.165
599.0292 599/.0292 |
| Altri autori (Persone) | ElschnerMandy C |
| Soggetto topico |
Pathogenic microorganisms - Analysis
Pathogenic microorganisms - Detection Bacterial diseases - Epidemiology Bacterial diseases - Microbiology Molecular epidemiology Biotechnology - Safety measures Biological decontamination |
| ISBN |
3-527-64509-8
1-280-78270-6 9786613693099 3-527-64510-1 3-527-64511-X |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | pt. A. Pathogens -- pt. B. Practical guidelines. |
| Record Nr. | UNINA-9910137629303321 |
| Weinheim, Germany, : Wiley-Blackwell, c2012 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
BSL3 and BSL4 agents : epidemiology, microbiology, and practical guidelines / / edited by Mandy C. Elschner ... [et al.]
| BSL3 and BSL4 agents : epidemiology, microbiology, and practical guidelines / / edited by Mandy C. Elschner ... [et al.] |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | Weinheim, Germany, : Wiley-Blackwell, c2012 |
| Descrizione fisica | 1 online resource (401 p.) |
| Disciplina |
579.165
599.0292 599/.0292 |
| Altri autori (Persone) | ElschnerMandy C |
| Soggetto topico |
Pathogenic microorganisms - Analysis
Pathogenic microorganisms - Detection Bacterial diseases - Epidemiology Bacterial diseases - Microbiology Molecular epidemiology Biotechnology - Safety measures Biological decontamination |
| ISBN |
3-527-64509-8
1-280-78270-6 9786613693099 3-527-64510-1 3-527-64511-X |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | pt. A. Pathogens -- pt. B. Practical guidelines. |
| Record Nr. | UNINA-9910821610303321 |
| Weinheim, Germany, : Wiley-Blackwell, c2012 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Dairy management practices and the transmission of zoonotic pathogens in milk ... annual report
| Dairy management practices and the transmission of zoonotic pathogens in milk ... annual report |
| Pubbl/distr/stampa | Washington, D.C., : U.S. Dept. of Agriculture, Agricultural Research Service |
| Descrizione fisica | : HTML files |
| Disciplina | 363 |
| Soggetto topico |
Cows - Pathogens - United States
Pathogenic microorganisms - Ecology - United States Pathogenic microorganisms - Detection - United States Milk contamination - United States - Prevention Raw milk - United States - Safety measures Pathogenic microorganisms - Detection |
| Soggetto genere / forma | Periodicals. |
| ISSN | 1948-5301 |
| Formato | Materiale a stampa |
| Livello bibliografico | Periodico |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910698685703321 |
| Washington, D.C., : U.S. Dept. of Agriculture, Agricultural Research Service | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Detection of highly dangerous pathogens [[electronic resource] ] : microarray methods for BSL 3 and BSL 4 agents / / edited by Tanja Kostic, Patrick Butaye, and Jacques Schrenzel
| Detection of highly dangerous pathogens [[electronic resource] ] : microarray methods for BSL 3 and BSL 4 agents / / edited by Tanja Kostic, Patrick Butaye, and Jacques Schrenzel |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, 2009 |
| Descrizione fisica | 1 online resource (193 p.) |
| Disciplina | 579.165 |
| Altri autori (Persone) |
KosticTanja
ButayePatrick SchrenzelJacques |
| Soggetto topico |
Pathogenic microorganisms - Detection
DNA microarrays |
| ISBN |
1-282-29205-6
9786612292057 3-527-62668-9 3-527-62669-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Detection of Highly Dangerous Pathogens: Microarray Methods for the Detection of BSL 3 and BSL 4 Agents; Contents; This Publication is Supported by COST; Preface; List of Contributors; 1 Introduction to Microarray-Based Detection Methods; 1.1 Introduction to Microarray Technology; 1.2 Technical Aspects of Microarray Technology; 1.2.1 Probes; 1.2.1.1 Genome Fragments; 1.2.1.2 PCR Products; 1.2.1.3 Oligonucleotide Probes; 1.2.2 Substrates for Printing; 1.2.2.1 Slides with Poly-L-lysine Coating; 1.2.2.2 Slides with Amino Silane Coating; 1.2.2.3 Slides with Aldehyde Coating
1.2.2.4 Slides with Epoxy Coating1.2.2.5 Proprietary Surface Chemistries; 1.2.2.6 Probe Spacers; 1.2.3 Targets for Microarray Analysis; 1.2.3.1 Target Amplifications and Sensitivity Issues; 1.2.3.2 Labeling of the Targets; 1.2.3.3 Hybridization and Wash Conditions; 1.2.4 Classical Commercially Available Microarray Formats; 1.2.4.1 Spotting Approaches; 1.2.4.2 In Situ Synthesis; 1.2.5 Alternative Methods for Improving Microarray-Based Detection Sensitivity; 1.2.5.1 Resonance-Light Scattering (RLS); 1.2.5.2 Planar-Waveguide Technology (PWT); 1.2.5.3 Liquid Arrays 1.2.5.4 Three-Dimensional Microarray Formats1.2.6 Marker Genes Used on MDMs; 1.3 Analysis and Quality Control Aspects; 1.4 Applications of Microarray Technology in Microbial Diagnostics; 1.4.1 Gene Expression Studies; 1.4.2 Comparative Genomic Hybridization (CGH); 1.4.3 Generic or Universal Microarrays; 1.4.4 Microarrays for Sequence Analysis; 1.4.5 Microbial Diagnostic Microarrays (MDMs); 1.5 Further Developments and New Perspectives Regarding Array Sensitivity and Specificity; 1.6 Conclusions; References; Part I: Methods; 2 Long Oligonucleotide Microarray-Based Microbial Detection 2.1 Introduction2.2 Method; 2.2.1 DNA Extraction; 2.2.2 Ф29 Amplification; 2.2.3 Klenow Amplification/Labeling; 2.2.4 Probe and Slide Preparation; 2.2.5 Slide Processing Protocol (for Amino Surfaces); 2.2.6 Hybridization and Slide Washing; 2.2.7 Comments; 2.3 Our Test System and Results; 2.4 Conclusions; References; 3 Sequence-Specific End-Labeling of Oligonucleotides; 3.1 Introduction; 3.2 Probe Design; 3.3 Slide Preparation (Spotting); 3.4 Slide Processing Protocol ( for Aldehyde Surfaces); 3.5 DNA Extraction and PCR Amplification of the Targeted Gene 3.6 Shrimp Alkaline Phosphatase Treatment3.7 Labeling; 3.8 Hybridization and Slide Washing; 3.9 Data Analysis; 3.10 Costs; 3.11 Microarray for Detection of Pathogenic Bacteria; References; 4 Non-Cognate Approaches for Pathogen Detection on Microarrays; 4.1 Introduction; 4.2 Non-Cognate Hybridization System; 4.2.1 Concept; 4.2.2 Definition of the Optimal Probe Length; 4.2.3 Virtual Assessment of Array Performances (in Silico Experiments); 4.2.4 Array Manufacturing and Hybridization (Wet-Lab Experiments); 4.2.5 Analysis; 4.3 Perspectives; References; 5 Patterning Techniques for Array Platforms 5.1 Introduction |
| Record Nr. | UNINA-9910143129503321 |
| Weinheim, : Wiley-VCH, 2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Detection of highly dangerous pathogens [[electronic resource] ] : microarray methods for BSL 3 and BSL 4 agents / / edited by Tanja Kostic, Patrick Butaye, and Jacques Schrenzel
| Detection of highly dangerous pathogens [[electronic resource] ] : microarray methods for BSL 3 and BSL 4 agents / / edited by Tanja Kostic, Patrick Butaye, and Jacques Schrenzel |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, 2009 |
| Descrizione fisica | 1 online resource (193 p.) |
| Disciplina | 579.165 |
| Altri autori (Persone) |
KosticTanja
ButayePatrick SchrenzelJacques |
| Soggetto topico |
Pathogenic microorganisms - Detection
DNA microarrays |
| ISBN |
1-282-29205-6
9786612292057 3-527-62668-9 3-527-62669-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Detection of Highly Dangerous Pathogens: Microarray Methods for the Detection of BSL 3 and BSL 4 Agents; Contents; This Publication is Supported by COST; Preface; List of Contributors; 1 Introduction to Microarray-Based Detection Methods; 1.1 Introduction to Microarray Technology; 1.2 Technical Aspects of Microarray Technology; 1.2.1 Probes; 1.2.1.1 Genome Fragments; 1.2.1.2 PCR Products; 1.2.1.3 Oligonucleotide Probes; 1.2.2 Substrates for Printing; 1.2.2.1 Slides with Poly-L-lysine Coating; 1.2.2.2 Slides with Amino Silane Coating; 1.2.2.3 Slides with Aldehyde Coating
1.2.2.4 Slides with Epoxy Coating1.2.2.5 Proprietary Surface Chemistries; 1.2.2.6 Probe Spacers; 1.2.3 Targets for Microarray Analysis; 1.2.3.1 Target Amplifications and Sensitivity Issues; 1.2.3.2 Labeling of the Targets; 1.2.3.3 Hybridization and Wash Conditions; 1.2.4 Classical Commercially Available Microarray Formats; 1.2.4.1 Spotting Approaches; 1.2.4.2 In Situ Synthesis; 1.2.5 Alternative Methods for Improving Microarray-Based Detection Sensitivity; 1.2.5.1 Resonance-Light Scattering (RLS); 1.2.5.2 Planar-Waveguide Technology (PWT); 1.2.5.3 Liquid Arrays 1.2.5.4 Three-Dimensional Microarray Formats1.2.6 Marker Genes Used on MDMs; 1.3 Analysis and Quality Control Aspects; 1.4 Applications of Microarray Technology in Microbial Diagnostics; 1.4.1 Gene Expression Studies; 1.4.2 Comparative Genomic Hybridization (CGH); 1.4.3 Generic or Universal Microarrays; 1.4.4 Microarrays for Sequence Analysis; 1.4.5 Microbial Diagnostic Microarrays (MDMs); 1.5 Further Developments and New Perspectives Regarding Array Sensitivity and Specificity; 1.6 Conclusions; References; Part I: Methods; 2 Long Oligonucleotide Microarray-Based Microbial Detection 2.1 Introduction2.2 Method; 2.2.1 DNA Extraction; 2.2.2 Ф29 Amplification; 2.2.3 Klenow Amplification/Labeling; 2.2.4 Probe and Slide Preparation; 2.2.5 Slide Processing Protocol (for Amino Surfaces); 2.2.6 Hybridization and Slide Washing; 2.2.7 Comments; 2.3 Our Test System and Results; 2.4 Conclusions; References; 3 Sequence-Specific End-Labeling of Oligonucleotides; 3.1 Introduction; 3.2 Probe Design; 3.3 Slide Preparation (Spotting); 3.4 Slide Processing Protocol ( for Aldehyde Surfaces); 3.5 DNA Extraction and PCR Amplification of the Targeted Gene 3.6 Shrimp Alkaline Phosphatase Treatment3.7 Labeling; 3.8 Hybridization and Slide Washing; 3.9 Data Analysis; 3.10 Costs; 3.11 Microarray for Detection of Pathogenic Bacteria; References; 4 Non-Cognate Approaches for Pathogen Detection on Microarrays; 4.1 Introduction; 4.2 Non-Cognate Hybridization System; 4.2.1 Concept; 4.2.2 Definition of the Optimal Probe Length; 4.2.3 Virtual Assessment of Array Performances (in Silico Experiments); 4.2.4 Array Manufacturing and Hybridization (Wet-Lab Experiments); 4.2.5 Analysis; 4.3 Perspectives; References; 5 Patterning Techniques for Array Platforms 5.1 Introduction |
| Record Nr. | UNINA-9910831037603321 |
| Weinheim, : Wiley-VCH, 2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Detection of pathogens in water using micro and nano-technology [[electronic resource] /] / [editors] Giampaolo Zuccheri and Nikolaos Asproulis
| Detection of pathogens in water using micro and nano-technology [[electronic resource] /] / [editors] Giampaolo Zuccheri and Nikolaos Asproulis |
| Autore | Zuccheri Giampaolo |
| Pubbl/distr/stampa | London ; ; New York, : IWA Publishing, 2012 |
| Descrizione fisica | 1 online resource (316 p.) |
| Disciplina | 628.161 |
| Altri autori (Persone) |
ZuccheriGiampaolo
AsproulisNikolaos |
| Soggetto topico |
Water - Microbiology
Pathogenic microorganisms - Detection Nanotechnology Microtechnology |
| Soggetto non controllato |
Technology & Engineering
Environmental General Science Applied Sciences Environmental Science (see Also Chemistry Environmental) |
| ISBN | 1-78040-109-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Contents -- Preface -- Contributors -- Chapter 1: Overview of European regulation and standards on microbiological water analysis / Sabine Müller and Jonathan Loeffler -- 1.1. Introduction -- 1.2. European Regulation on Microbiological Analysis of Drinking Water -- 1.3. European Regulation on Microbiological Analysis of Recreational Water -- 1.4. European and International Standards for Microbiological Water Analysis -- Chapter 2: Risk analysis of bio-terroristic attacks on drinking water systems / Christian Mittermayr -- 2.1. Introduction -- 2.2. Definitions -- 2.3. Risk Analysis for Terrorism -- 2.4. Risk Analysis of Bio-Terroristic Attacks on Drinking Water Systems -- 2.5. Risk Estimation -- Chapter 3: Sample collection procedures for Online Contaminant Monitoring System / Miloslava Proksˇova´, Marianna Cíchová and Lívia Tóthová-- 3.1. Introduction -- 3.2. Microbial Monitoring of Drinking Water -- 3.3. Sampling Plan -- 3.4. New Security Approaches for Drinking Water -- 3.5. New Approach of Online Contamination Monitoring Device (OCMD) -- 3.5.1. Location of OCMD in water system -- Chapter 4: A device to extract highly diluted specimens out of large volumes of water for analysis in lab-on-a-chip detection systems / Christoph Zeis -- 4.1. The Need for a Macro-to-Micro-Fluidic Interface -- 4.2. Principles of Separation -- 4.3. The Needle in the Haystack -- 4.4. Technical Description of the DINAMICS Concentration Apparatus -- 4.5. Conclusions -- Chapter 5: Sustainable DNA/RNA release methods for in-line waterborne pathogen screening devices / Hunor Sántha -- 5.1. Introduction -- 5.2. Survey on Pathogen-Lysis/Cell-Disruption Methods -- 5.3 Considerations for Water Samples and In-line (Quasi Continuous) operation -- 5.4 The Realised Case in The DINAMICS Project -- Chapter 6: The microsystem based core of the DINAMICS water testing system: Design considerations and realization of the chip units / Theo T. Veenstra -- 6.1 Microfluidics in DINAMICS -- 6.2 Layout of the Fluidic Parts of DINAMICS -- 6.3 Microfluidic Components in DINAMICS -- 6.4 Current Status -- 6.5 Conclusion -- Chapter 7: Electrochemical biosensor strategies for pathogen detection in water security / Daniele Gazzola, Simone Bonetti and Giampaolo Zuccheri -- 7.1 Introduction -- 7.2 Biosensor Strategies for Pathogen Detection in Water Security -- 7.3 Common Electrochemical Detection Systems for DNA Biosensors -- 7.4 The On-Chip Simplified Electrochemical Technique Developed in the DINAMICS Project -- Chapter 8: Biochemical and Nanotechnological Strategies for Signal Enhancement in the Detection of Nucleic Acids with Biosensors / Alessandra Vinelli, Manuele Onofri and Giampaolo Zuccheri8.1 Introduction -- 8.2 Enhancement Methods Based on Enzymatic (or Catalyzed) Reactions -- 8.3 Enhancement Methods Based on Nanoparticles or Nanostructures -- 8.4 Method Based on DNA Nanostructures -- 8.5 Conclusions and Perspectives -- Chapter 9: Computational modelling of aqueous environments in micro and nanochannels / D. Mantzalis, K. Karantonis, N. Asproulis, L. Könözsy and D. Drikakis -- 9.1 Introduction -- 9.2 Effects of Physical Characteristics -- 9.3 Computational Approaches -- 9.4 Liquid Flow in Confined Geometries -- Chapter 10: Computational recipes of transport phenomena in micro and nanofluidics / N. Asproulis10.1 Introduction -- 10.2 Modelling Approaches -- 10.3 Meta-Modelling for Macromolecules -- 10.4 Hybrid Continuum-Molecular Models -- References -- Chapter 11: Multi-detection of waterborne pathogens in raw and treated water samples by using ultrafiltration concentration and DNA array technology / Sophie Courtois, Anne Cajon, Aurore Romey, Fanny Poyet and Claude Mabilat -- 11.1 Introduction -- 11.2 Improved and Simplified Method for Concentrating Viral, Bacterial and Protozoan Pathogens -- 11.3 Integrated Protocol for Nucleic Acid Extraction, Amplification and Sequence Identification Through High Density Microarray -- 11.4 Results -- 11.5 Conclusions -- References -- Chapter 12: Detection and enumeration of waterborne mycobacteria / Joseph O. Falkinham III -- 12.1 Ecology of Waterborne Mycobacteria -- 12.2 Physiological Ecology of Waterborne Mycobacteria -- 12.3 Risk Analysis and Source-Tracking Environmental Mycobacteria -- 12.4 Sampling and Sample Treatment Strategies for Mycobacterial Detection and Enumeration -- 12.5 Mycobacterial Detection or Enumeration -- Chapter 13: New molecular technologies for the rapid detection of Legionella in water / E. Soria, M. A. Yáñez, R. Múrtula and V. Catalán -- 13.1 Introduction -- 13.2 Immunodetection and Legionella Fast Detection -- 13.3 Legionella Detection using Microfluidics -- 13.4 Future Research Directions -- Chapter 14: Detection of virus in the water environment / Johan Nordgren and Lennart Svensson -- 14.1 Introduction -- 14.2 Concentration of Virus from Water Samples -- 14.3 Detection and Quantification Methods -- 14.4 Perspectives -- References -- Chapter 15: Design of PCR primers for the detection of waterborne bacteria / Julien Gardès and Richard Christen -- 15.1 Introduction -- 15.2 The Target Genes -- 15.3 Design of PCR Primers -- 15.4 DNA-Based Detection Technologies -- 15.5 Conclusions -- References -- Chapter 16: Fluid structure and boundary slippage in nanoscale liquid films / Nikolai V. Priezjev -- 16.1 Abstract -- 16.2 Introduction -- 16.3 Molecular Dynamics Simulation Model -- 16.4 Results -- 16.5 Conclusions -- References -- Chapter 17: Understanding slip at the nanoscale in fluid flows using atomistic simulations / T. E. Karakasidis and A. Liakopoulos -- 17.1 Introduction – Definition of Slip -- 17.2 Importance of Slip -- 17.3 Experimental Measurement of Slip -- 17.4 Atomistic Simulations -- 17.5 Atomistic Simulations Results About Slip -- 17.6 Conclusions -- References. |
| Record Nr. | UNINA-9910433257103321 |
Zuccheri Giampaolo
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| London ; ; New York, : IWA Publishing, 2012 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Develop spectroscopy techniques for detection of trace bacteria and biological toxins in foods ... annual report
| Develop spectroscopy techniques for detection of trace bacteria and biological toxins in foods ... annual report |
| Pubbl/distr/stampa | Washington, D.C., : U.S. Dept. of Agriculture, Agricultural Research Service |
| Descrizione fisica | : HTML files |
| Disciplina | 363 |
| Soggetto topico |
Raman effect, Surface enhanced
Spectrum analysis Pathogenic microorganisms - Detection - United States Food adulteration and inspection - United States Nanochemistry Food adulteration and inspection Pathogenic microorganisms - Detection |
| Soggetto genere / forma | Periodicals. |
| ISSN | 2153-7577 |
| Formato | Materiale a stampa |
| Livello bibliografico | Periodico |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910698481003321 |
| Washington, D.C., : U.S. Dept. of Agriculture, Agricultural Research Service | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Development of nanotechnology for rapid detection of food pathogens ... annual report
| Development of nanotechnology for rapid detection of food pathogens ... annual report |
| Pubbl/distr/stampa | [Washington, D.C.], : U.S. Dept. of Agriculture, Agricultural Research Service |
| Descrizione fisica | : HTML files |
| Disciplina | 363.192 |
| Soggetto topico |
Nanotechnology
Pathogenic microorganisms - Detection Food contamination |
| Soggetto genere / forma | Periodicals. |
| ISSN | 2378-2382 |
| Formato | Materiale a stampa |
| Livello bibliografico | Periodico |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910703113403321 |
| [Washington, D.C.], : U.S. Dept. of Agriculture, Agricultural Research Service | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Genomic sequencing of infectious pathogens
| Genomic sequencing of infectious pathogens |
| Pubbl/distr/stampa | [Washington, D.C.] : , : GAO - Science, Technology Assessment, and Analytics, , [2021] |
| Descrizione fisica | 1 online resource (2 unnumbered pages) : color illustrations |
| Collana | Science & tech spotlight |
| Soggetto topico |
Genomics
Pathogenic microorganisms - Detection Coronaviruses - Genetics Coronaviruses - Reproduction Communicable diseases - Transmission - Prevention |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910715980403321 |
| [Washington, D.C.] : , : GAO - Science, Technology Assessment, and Analytics, , [2021] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Improved detection techniques for foodborne pathogens ... annual report
| Improved detection techniques for foodborne pathogens ... annual report |
| Pubbl/distr/stampa | Washington, D.C., : U.S. Dept. of Agriculture, Agricultural Research Service |
| Descrizione fisica | : HTML files |
| Disciplina | 363 |
| Soggetto topico |
Pathogenic microorganisms - Detection - United States
Biosensors - United States Food contamination - United States - Prevention Biosensors Food contamination - Prevention Pathogenic microorganisms - Detection |
| Soggetto genere / forma | Periodicals. |
| ISSN | 2153-7593 |
| Formato | Materiale a stampa |
| Livello bibliografico | Periodico |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910698480803321 |
| Washington, D.C., : U.S. Dept. of Agriculture, Agricultural Research Service | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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