Pubbl/distr/stampa	[Larchmont, N.Y.], : Mary Ann Liebert, 1990-
Disciplina	574
Soggetto topico	DNA Cytology Genes Genetics - Technique Nucleic acids Recombinant DNA Genetic Techniques Nucleic Acids ADN recombinant ADN Cytologie Gènes Génétique - Technique Acides nucléiques Histology Zeitschrift Celbiologie DNS
Soggetto genere / forma	Periodical Periodicals.
ISSN	1557-7430
Formato	Materiale a stampa
Livello bibliografico	Periodico
Lingua di pubblicazione	eng
Record Nr.	UNISA-996213086803316

Autore	Gjerde Douglas T
Pubbl/distr/stampa	[Place of publication not identified], : Wiley VCH, 2002
Descrizione fisica	1 online resource (238 pages)
Disciplina	572.8/6
Soggetto topico	DNA - Analysis Chromatographic analysis DNA Chromatography, High Pressure Liquid Genetic Techniques Investigative Techniques Nucleic Acids Chromatography, Liquid Nucleic Acids, Nucleotides, and Nucleosides Analytical, Diagnostic and Therapeutic Techniques and Equipment Chromatography Chemistry Techniques, Analytical Chemicals and Drugs Animal Biochemistry Human Anatomy & Physiology Health & Biological Sciences
Soggetto genere / forma	Electronic books.
ISBN	1-280-55844-X 9786610558445 3-527-60074-4
Formato	Materiale a stampa
Livello bibliografico	Monografia
Lingua di pubblicazione	eng
Record Nr.	UNINA-9910146247903321

Autore	Dale Jeremy (Jeremy W.)
Edizione	[2nd ed.]
Pubbl/distr/stampa	Hoboken, : Wiley, 2008
Descrizione fisica	1 online resource (396 p.)
Disciplina	572.8 572.86 660.65
Altri autori (Persone)	von SchantzMalcolm
Soggetto topico	Cloning, Molecular DNA, Recombinant DNA Genes Genetic Engineering Genomes Genetic engineering Genome Genetic Structures Genetic Techniques Investigative Techniques Genetic Phenomena Nucleic Acids Analytical, Diagnostic and Therapeutic Techniques and Equipment Phenomena and Processes Nucleic Acids, Nucleotides, and Nucleosides Chemicals and Drugs Genetics Biology Health & Biological Sciences
Soggetto genere / forma	Electronic books.
ISBN	0-470-85690-4 1-282-34827-2 0-470-51870-7 0-470-01734-1 9786612348273 1-280-62202-4 9786610622023 0-470-85691-2
Formato	Materiale a stampa
Livello bibliografico	Monografia
Lingua di pubblicazione	eng
Nota di contenuto	""From Genes to Genomes""; ""Contents""; ""Preface""; ""1: Introduction""; ""2: Basic Molecular Biology""; ""2.1 Nucleic acid structure""; ""2.2 What is a gene?""; ""2.3 Information flow: gene expression""; ""2.4 Gene structure and organization""; ""3: How to Clone a Gene""; ""3.1 What is cloning?""; ""3.2 Overview of the procedures""; ""3.3 Gene libraries""; ""3.4 Hybridization""; ""3.5 Polymerase chain reaction""; ""3.6 Extraction and purification of nucleic acids""; ""3.7 Detection and quantitation of nucleic acids""; ""3.8 Gel electrophoresis""; ""4: Cutting and Joining DNA"" ""4.1 Restriction endonucleases""""4.2 Ligation""; ""4.3 Modification of restriction fragment ends""; ""4.4 Other ways of joining DNA molecules""; ""5: Vectors""; ""5.1 Plasmid vectors""; ""5.2 Vectors based on the lambda bacteriophage""; ""5.3 Cosmids""; ""5.4 M13 vectors""; ""5.5 Expression vectors""; ""5.6 Vectors for cloning and expression in eukaryotic cells""; ""5.7 Supervectors: YACs and BACs""; ""5.8 Summary""; ""6: Genomic and cDNA Libraries""; ""6.1 Genomic libraries""; ""6.2 Growing and storing libraries""; ""6.3 cDNA libraries""; ""6.4 Random, arrayed and ordered libraries"" ""7: Finding the Right Clone""""7.1 Screening libraries with gene probes""; ""7.2 Screening expression libraries with antibodies""; ""7.3 Subcloning""; ""7.4 Characterization of plasmid clones""; ""8: Polymerase Chain Reaction""; ""8.1 The PCR reaction""; ""8.2 PCR in practice""; ""8.3 Cloning PCR products""; ""8.4 Long-range PCR""; ""8.5 Reverse-transcription PCR""; ""8.6 Rapid amplification of cDNA ends""; ""8.7 Quantitative PCR""; ""8.8 Applications of PCR""; ""9: Characterization of a Cloned Gene""; ""9.1 DNA sequencing""; ""9.2 Databank entries and annotation""; ""9.3 Sequence analysis"" ""9.4 Sequence comparisons""""9.5 Protein structure""; ""9.6 Confirming gene function""; ""10: Analysis of Gene Expression""; ""10.1 Analysing transcription""; ""10.2 Methods for studying the promoter""; ""10.3 Regulatory elements and DNA-binding proteins""; ""10.4 Translational analysis""; ""11: Products from Native and Manipulated Cloned Genes""; ""11.1 Factors affecting expression of cloned genes""; ""11.2 Expression of cloned genes in bacteria""; ""11.3 Expression in eukaryotic host cells""; ""11.4 Adding tags and signals""; ""11.5 In vitro mutagenesis""; ""11.6 Vaccines"" ""12: Genomic Analysis""""12.1 Genome sequencing""; ""12.2 Analysis and annotation""; ""12.3 Comparing genomes""; ""12.4 Genome browsers""; ""12.5 Relating genes and functions: genetic and physical maps""; ""12.6 Transposon mutagenesis and other screening techniques""; ""12.7 Conclusion""; ""13: Analysis of Genetic Variation""; ""13.1 Single nucleotide polymorphisms""; ""13.2 Larger-scale variations""; ""13.3 Other methods for studying variation""; ""13.4 Human genetic diseases""; ""13.5 Molecular phylogeny""; ""14: Post-genomic Analysis""; ""14.1 Analysing transcription; transcriptomes"" ""14.2 Array-based methods""
Record Nr.	UNINA-9910143510103321

Pubbl/distr/stampa	London, : SAGE-Hindawi Access to Research
Descrizione fisica	1 online resource
Soggetto topico	Nucleic acids Nucleic Acids
Soggetto genere / forma	Periodicals. Periodical
Soggetto non controllato	Animal Biochemistry
ISSN	2090-021X
Formato	Materiale a stampa
Livello bibliografico	Periodico
Lingua di pubblicazione	eng
Record Nr.	UNISA-996199486703316

Autore	Mori Keita
Edizione	[1st ed. 2024.]
Pubbl/distr/stampa	Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2024
Descrizione fisica	1 online resource (137 pages)
Disciplina	620.19 547.7
Collana	Springer Theses, Recognizing Outstanding Ph.D. Research
Soggetto topico	Biomaterials Nucleic acids Coordination compounds Nanotechnology Self-assembly (Chemistry) Biocompatible Materials Nucleic Acids Layer-by-Layer Nanoparticles Nucleic Acid Coordination Chemistry Self-assembly
ISBN	981-9994-00-4
Formato	Materiale a stampa
Livello bibliografico	Monografia
Lingua di pubblicazione	eng
Nota di contenuto	1. General introduction -- 2. Metal-responsive DNA strand displacement reactions driven by base pair switching of 5-hydroxyuracil nucleobases -- 3. Metal-dependent base pair switching of N,N,-dicarboxymethyl-5-aminouracil nucleosides -- 4. Metal-responsive DNA tweezers driven by base pair switching of 5-hydroxyuracil nucleobases -- 5. Conclusion and perspectives.
Record Nr.	UNINA-9910831014603321