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Antisense RNA Design, Delivery, and Analysis / / edited by Virginia Arechavala-Gomeza, Alejandro Garanto
Antisense RNA Design, Delivery, and Analysis / / edited by Virginia Arechavala-Gomeza, Alejandro Garanto
Autore Arechavala-Gomeza Virginia
Edizione [1st ed. 2022.]
Pubbl/distr/stampa New York, : Springer Nature, 2022
Descrizione fisica 1 online resource (XVII, 422 p. 69 illus., 59 illus. in color.)
Disciplina 615.5
Collana Methods in Molecular Biology
Soggetto topico Therapeutics
Biomaterials
Nucleic acids
Nucleic Acid
Àcids nuclèics
RNA
Teràpia genètica
Soggetto genere / forma Llibres electrònics
Soggetto non controllato RNA therapeutics
Antisense technology
Therapeutic design
Oligonucleotides
Model systems
Oligonucleotide-mediated toxicology
Open Access
ISBN 1-0716-2010-X
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Introduction and History of the Chemistry of Nucleic Acids Therapeutics -- Antisense RNA Therapeutics: A Brief Overview -- Design of Bifunctional Antisense Oligonucleotides for Exon Inclusion -- Design and Delivery of SINEUP: A New Modular Tool to Increase Protein Translation -- How to Design U1 snRNA Molecules for Splicing Rescue -- Conjugation of Nucleic Acids and Drugs to Gold Nanoparticles -- Determination of Optimum Ratio of Cationic Polymers and Small Interfering RNA with Agarose Gel Retardation Assay -- Generation of Protein-Phosphorodiamidate Morpholino Oligomer Conjugates for Efficient Cellular Delivery via Anthrax Protective Antigen -- Development and Use of Cellular Systems to Assess and Correct Splicing Defects -- Modeling Splicing Variants Amenable to Antisense Therapy by Use of CRISPR-Cas9-Based Gene Editing in HepG2 Cells -- In Vitro Models for the Evaluation of Antisense Oligonucleotides in Skin -- In Vitro Delivery of PMOs in Myoblasts by Electroporation -- Rapid Determination of MBNL1 Protein Levels by Quantitative Dot Blot for Evaluation of Antisense Oligonucleotides in Myotonic Dystrophy Myoblasts -- Evaluation of Exon Skipping and Dystrophin Restoration in In Vitro Models of Duchenne Muscular Dystrophy -- Generation of Human iPSC-Derived Myotubes to Investigate RNA-Based Therapies In Vitro -- Eye on a Dish Models to Evaluate Splicing Modulation -- Establishment of In Vitro Brain Models for AON Delivery -- Considerations for Generating Humanized Mouse Models to Test Efficacy of Antisense Oligonucleotides -- Generation of Humanized Zebrafish Models for the In Vivo Assessment of Antisense Oligonucleotide-Based Splice Modulation Therapies -- Use of Small Animal Models for Duchenne and Parameters to Assess Efficiency upon Antisense Treatment -- In Vivo Models for the Evaluation of Antisense Oligonucleotides in Skin -- Delivery of Antisense Oligonucleotides to the Mouse Retina -- Delivery of Antisense Oligonucleotides to the Mouse Brain by Intracerebroventricular Injections -- Intrathecal Delivery of Therapeutic Oligonucleotides for Potent Modulation of Gene Expression in the Central Nervous System -- Preclinical Safety Assessment of Therapeutic Oligonucleotides -- Preclinical Evaluation of the Renal Toxicity of Oligonucleotide Therapeutics in Mice -- Protocol for Isolation and Culture of Mouse Hepatocytes (HCs), Kupffer Cells (KCs), and Liver Sinusoidal Endothelial Cells (LSECs) in Analyses of Hepatic Drug Distribution -- Patent Considerations When Embarking on New Antisense Drug Programs.
Record Nr. UNINA-9910552988503321
Arechavala-Gomeza Virginia  
New York, : Springer Nature, 2022
Materiale a stampa
Lo trovi qui: Univ. Federico II
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Handbook of Chemical Biology of Nucleic Acids [[electronic resource] /] / edited by Naoki Sugimoto
Handbook of Chemical Biology of Nucleic Acids [[electronic resource] /] / edited by Naoki Sugimoto
Autore Sugimoto Naoki
Edizione [1st ed. 2023.]
Pubbl/distr/stampa Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2023
Descrizione fisica 1 online resource (2847 pages)
Disciplina 620.19
547.7
Soggetto topico Biomaterials
Nucleic acids
Biochemistry
Chemistry, Organic
Biotechnology
Nanochemistry
Medicine - Research
Biology - Research
Nucleic Acid
Chemical Biology
Organic Chemistry
Biomedical Research
ISBN 981-19-9776-4
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto High-Pressure Single-Molecule Studies on Non-canonical Nucleic Acids and Their Interactions -- Stability Prediction of Canonical and Noncanonical Structures of Nucleic Acids -- The Effect of Pressure on the Conformational Stability of DNA -- Quadruplexes are everywhere…on the other strand too: the i-motif -- i-Motif Nucleic Acids -- NMR Study on Nucleic Acids -- Z-DNA -- Structures of DNA G-quadruplexes and Their Drug Interactions -- In cell 19F NMR for G-quadruplex -- Structures and Catalytic Activities of Complexes between Heme and DNA -- Studying nucleic acid-ligand binding by X-ray crystallography -- Predicting the 3D structure of RNA from sequence -- XNA in Synthetic Biology -- The Effects of FANA Modifications on Non-Canonical Nucleic Acid Structures -- Isomorphic Fluorescent Nucleoside Analogs -- Bridged Nucleic Acids for Therapeutic Oligonucleotides -- Mesyl Phosphoramidate Oligonucleotides: A New Promising Type of Antisense Agents -- Chemistry of Cyclic Dinucleotides and Analogs -- Labeling and Detection of Modified Nucleic Acids -- Cross linking Duplex of Nucleic Acids with Modified Oligonucleotides -- Enzymatic Synthesis of Base-Modified Nucleic Acids -- Charge Transfer in Natural and Artificial Nucleic Acids -- Nucleic Acid Aptamers: From Basic Research to Clinical Applications -- LNA in Medical Biology -- Targeting Quadruplex Nucleic Acids: The Bisquinolinium Saga -- Compound Shape and Substituent Effects in DNA Minor Groove Interactions -- Macrocyclic G-Quadruplex Ligands of Telomestatin Analogs -- Cyclic Naphthalene Diimide Derivatives as Novel DNA Ligands -- Imaging study of small molecules to G-quadruplexes in cells -- Bivalent Nucleic Acid Ligands -- DNA/Metal Cluster–Based Nano-Lantern -- Interaction of Poly (ethylene glycol)-b-poly-L-lysine Copolymers with DNA Structures: A Thermodynamic Investigation -- Chemical tools to target non-coding RNAs -- Targeting DNA junctions with small molecules for therapeutic applications in oncology -- DNA Damage and Repair in G-quadruplexes Impact Gene Expression -- DNA Structural Elements as Potential Targets for Regulation of Gene Expression -- Effects of molecular crowding on structures and functions of nucleic acids -- Structure-guided Optimization of siRNA and anti-miRNA Properties -- Tools for understanding the chemical biology of the tRNA epitranscriptome -- Sulfur and Selenium modified bacterial tRNAs -- Chemical-assisted Epigenome Sequencing -- Epitranscriptomics. Telomeres and Telomerase -- Genetic Alphabet Expansion of Nucleic Acids -- Unnatural Base Pairs to Expand the Genetic Alphabet and Code -- OGG1 at the crossroads between repair and transcriptional regulation -- Phosphorothioate Nucleic Acids - Artificial Modification Envisaged by Nature -- Aptamer Molecular Evolution for Liquid Biopsy -- Functions and Applications of Riboswitches -- PNA and Quadruplex -- Peptide Ribonucleic Acid (PRNA)-Programmed Assemblies -- Structure and Function of Aptamer and Catalytic DNA -- Origin and Evolution of RNA -- Targeting RNA with Small Molecules.
Record Nr. UNINA-9910736011003321
Sugimoto Naoki  
Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2023
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Low-Cost Methods for Molecular Characterization of Mutant Plants : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols / / by Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger
Low-Cost Methods for Molecular Characterization of Mutant Plants : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols / / by Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger
Autore Till Bradley J
Edizione [1st ed. 2015.]
Pubbl/distr/stampa Cham : , : Springer International Publishing : , : Imprint : Springer, , 2015
Descrizione fisica 1 online resource (X, 35 p. 9 illus., 3 illus. in color.)
Disciplina 631.52
660.6
Soggetto topico Plant biotechnology
Biology—Technique
Biomaterials
Nucleic acids
Plant Biotechnology
Biological Techniques
Nucleic Acid
ISBN 3-319-16259-4
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Introduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions.  .
Record Nr. UNISA-996207426003316
Till Bradley J  
Cham : , : Springer International Publishing : , : Imprint : Springer, , 2015
Materiale a stampa
Lo trovi qui: Univ. di Salerno
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Low-Cost Methods for Molecular Characterization of Mutant Plants : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols / / by Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger
Low-Cost Methods for Molecular Characterization of Mutant Plants : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols / / by Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger
Autore Till Bradley J
Edizione [1st ed. 2015.]
Pubbl/distr/stampa Cham : , : Springer International Publishing : , : Imprint : Springer, , 2015
Descrizione fisica 1 online resource (X, 35 p. 9 illus., 3 illus. in color.)
Disciplina 631.52
660.6
Soggetto topico Plant biotechnology
Biology—Technique
Biomaterials
Nucleic acids
Plant Biotechnology
Biological Techniques
Nucleic Acid
ISBN 3-319-16259-4
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Introduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions.  .
Record Nr. UNINA-9910131313103321
Till Bradley J  
Cham : , : Springer International Publishing : , : Imprint : Springer, , 2015
Materiale a stampa
Lo trovi qui: Univ. Federico II
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Metal-Responsive Base Pair Switching of Ligand-type Uracil Nucleobases [[electronic resource] /] / by Keita Mori
Metal-Responsive Base Pair Switching of Ligand-type Uracil Nucleobases [[electronic resource] /] / by Keita Mori
Autore Mori Keita
Edizione [1st ed. 2024.]
Pubbl/distr/stampa Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2024
Descrizione fisica 1 online resource (137 pages)
Disciplina 620.19
547.7
Collana Springer Theses, Recognizing Outstanding Ph.D. Research
Soggetto topico Biomaterials
Nucleic acids
Coordination compounds
Nanotechnology
Self-assembly (Chemistry)
Biocompatible Materials
Nucleic Acids
Layer-by-Layer Nanoparticles
Nucleic Acid
Coordination Chemistry
Self-assembly
ISBN 981-9994-00-4
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto 1. General introduction -- 2. Metal-responsive DNA strand displacement reactions driven by base pair switching of 5-hydroxyuracil nucleobases -- 3. Metal-dependent base pair switching of N,N,-dicarboxymethyl-5-aminouracil nucleosides -- 4. Metal-responsive DNA tweezers driven by base pair switching of 5-hydroxyuracil nucleobases -- 5. Conclusion and perspectives.
Record Nr. UNINA-9910831014603321
Mori Keita  
Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2024
Materiale a stampa
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Molecular Techniques for Studying Viruses [[electronic resource] ] : Practical Notes / / by Zubaida Hassan, Gulfaraz Khan
Molecular Techniques for Studying Viruses [[electronic resource] ] : Practical Notes / / by Zubaida Hassan, Gulfaraz Khan
Autore Hassan Zubaida
Edizione [1st ed. 2024.]
Pubbl/distr/stampa Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2024
Descrizione fisica 1 online resource (105 pages)
Disciplina 570
Altri autori (Persone) KhanGulfaraz
Soggetto topico Biology
Biomaterials
Nucleic acids
Biological assay
Non-coding RNA
Bioinformatics
Biological Sciences
Nucleic Acid
Assay Systems
Non-coding RNAs
ISBN 981-9980-97-6
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Chapter 1. Introduction -- Chapter 2. Isolation of Nucleic Acids -- Chapter 3. Isolation of Proteins -- Chapter 4. PCR-Based Techniques -- Chapter 5. Western Blotting -- Chapter 6. Serological Assays - Chapter 7. Immunoprecipitation -- Chapter 8. Small Interfering RNA -- Chapter 9. Histological Methods -- Chapter 10. Bioinformatics and In Silico Stimulations. Chapter 11. Summary and Conclusions.
Record Nr. UNINA-9910838288903321
Hassan Zubaida  
Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2024
Materiale a stampa
Lo trovi qui: Univ. Federico II
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Nuclear, Chromosomal, and Genomic Architecture in Biology and Medicine [[electronic resource] /] / edited by Malgorzata Kloc, Jacek Z. Kubiak
Nuclear, Chromosomal, and Genomic Architecture in Biology and Medicine [[electronic resource] /] / edited by Malgorzata Kloc, Jacek Z. Kubiak
Edizione [1st ed. 2022.]
Pubbl/distr/stampa Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022
Descrizione fisica 1 online resource (657 pages)
Disciplina 571.835
Collana Results and Problems in Cell Differentiation
Soggetto topico Cell nuclei
Chromosomes
Biology - Technique
Genomics
Biomaterials
Nucleic acids
Gene expression
Nuclear Organization
Genomic Analysis
Nucleic Acid
Gene Expression Analysis
Citologia
Cromosomes
Genòmica
Nuclis cel·lulars
Diferenciació cel·lular
Soggetto genere / forma Llibres electrònics
ISBN 3-031-06573-5
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Part I. Genome Architecture, Evolution, and Cell Fate -- Chapter 1. Networks and Islands of Genome Nano-Architecture and their Potential Relevance for Radiation Biology (A Hypothesis and Experimental Verification Hints) -- Chapter 2. A Unified Genomic Mechanism of Cell-Fate Change -- Chapter 3. Alterations to Genome Organisation in Stem Cells, Their Differentiation and Associated Diseases -- Chapter 4. How Genomes Emerge, Function, and Evolve: Living Systems Emergence - Genotype-Phenotype-Multilism - Genome/Systems Ecology -- Chapter 5. Integrating Multimorbidity into a Whole-Body Understanding of Disease Using Spatial Genomics -- Part II. Chromosomes and Chromatin Architecture and Dynamics -- Chapter 6. Mitotic Antipairing of Homologous Chromosomes -- Chapter 7. CENP-A, a Histone H3 Variant with Key Roles in Centromere Architecture in Healthy and Diseased States -- Chapter 8. Scaling Relationship in Chromatin as a Polymer -- Chapter 9. Chromatin Dynamics during Entry to Quiescence and Compromised Functionality in Cancer Cells -- Chapter 10. Functional Aspects of Sperm Chromatin Organization -- Part III. Mechanosensitive and Epigenetic Regulators of Gene Expression and Chromatin Organization -- Chapter 11. The LINC Complex Assists the Nuclear Import of Mechanosensitive Transcriptional Regulators -- Chapter 12. Epigenetic-Mediated Regulation of Gene Expression for Biological Control and Cancer: Cell and Tissue Structure, Function, and Phenotype -- Chapter 13. Epigenetic-Mediated Regulation of Gene Expression for Biological Control and Cancer: Fidelity of Mechanisms Governing the Cell Cycle -- Chapter 14. Histone Modifications in Mouse Pronuclei and Consequences for Embryo Development -- Part IV. Nucleus, Nucleolus, and Nucleolar Organizer Architecture -- Chapter 15. Nuclear Architecture in the Nervous System -- Chapter 16. Nuclear Morphological Abnormalities in Cancer: A Search for Unifying Mechanisms -- Chapter 17. Nuclear Organization in Response to Stress: A Special Focus on Nucleoli -- Chapter 18. Simulation of Different Three-Dimensional Models of Whole Interphase Nuclei Compared to Experiment – A Consistent Scale-Bridging Simulation Framework for Genome Organization -- Chapter 19. Nucleolar Organizer Regions as Transcription-Based Scaffolds of Nucleolar Structure and Function -- Chapter 20. A Transient Mystery: Nucleolar Channel Systems -- Part V. Nuclear Actin Role in Polarization, Genome Organization, and Gene Expression -- Chapter 21. Cellular Polarity Transmission to the Nucleus -- Chapter 22. The Role of Nuclear Actin in Genome Organization and Gene Expression Regulation During Differentiation -- Chapter 23. Nuclear Actin Dynamics in Gene Expression, DNA Repair, and Cancer.
Record Nr. UNINA-9910629284303321
Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022
Materiale a stampa
Lo trovi qui: Univ. Federico II
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Progress in the Chemistry of Organic Natural Products 122 [[electronic resource] ] : Botanical Dietary Supplements and Herbal Medicines / / edited by A. Douglas Kinghorn, Heinz Falk, Simon Gibbons, Yoshinori Asakawa, Ji-Kai Liu, Verena M. Dirsch
Progress in the Chemistry of Organic Natural Products 122 [[electronic resource] ] : Botanical Dietary Supplements and Herbal Medicines / / edited by A. Douglas Kinghorn, Heinz Falk, Simon Gibbons, Yoshinori Asakawa, Ji-Kai Liu, Verena M. Dirsch
Autore Kinghorn A. Douglas
Edizione [1st ed. 2023.]
Pubbl/distr/stampa Cham : , : Springer Nature Switzerland : , : Imprint : Springer, , 2023
Descrizione fisica 1 online resource (298 pages)
Disciplina 615.321
Altri autori (Persone) FalkHeinz
GibbonsSimon
AsakawaYoshinori
LiuJi-Kai
DirschVerena M
Collana Progress in the Chemistry of Organic Natural Products
Soggetto topico Natural products
Biomaterials
Nucleic acids
Chemical structure
Natural Products
Nucleic Acid
Structure And Bonding
Plant Materials
ISBN 3-031-26768-0
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Phytochemical Profiles and Biological Studies of Selected Botanical Dietary Supplements Used in the United States -- Quality Consistency of Herbal Products: Chemical Evaluation -- Deoxyribonucleic Acid Barcoding for the Identification of Botanicals -- Botanical Pharmacognosy in Quality Control: A Focus on Raw Material Assessment.
Record Nr. UNINA-9910734822103321
Kinghorn A. Douglas  
Cham : , : Springer Nature Switzerland : , : Imprint : Springer, , 2023
Materiale a stampa
Lo trovi qui: Univ. Federico II
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Treasure Your Exceptions : The Science and Life of William Bateson / / by Alan G. Cock, Donald R. Forsdyke
Treasure Your Exceptions : The Science and Life of William Bateson / / by Alan G. Cock, Donald R. Forsdyke
Autore Forsdyke Donald R.
Edizione [2nd ed. 2022.]
Pubbl/distr/stampa Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022
Descrizione fisica 1 online resource (702 pages)
Disciplina 575.109
Soggetto topico Genetistes
Medical genetics
Medicine - History
Life sciences
Biology - Philosophy
Evolution (Biology)
Biomaterials
Nucleic acids
Medical Genetics
History of Medicine
Life Sciences
Philosophy of Biology
Evolutionary Biology
Nucleic Acid
Soggetto genere / forma Biografies
Llibres electrònics
ISBN 3-030-92099-2
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Abbreviations -- Prologue -- Part I. Genesis of a Geneticist -- 1 A Cambridge Childhood (1861-1882 -- 2 From Virginia to the Aral Sea (1883-1889) -- 3 Galton -- 4 Variation (1890-1894) -- 5 Romanes -- 6 Reorientation and Controversy (1895-1899) -- 7 What Life May Be -- Part II. Mendelism -- 8 Rediscovery (1900-1901) -- 9 Mendel’s Bulldog (1902-1906) -- 10 Bateson’s Bulldog -- 11 On Course (1907-1908) -- 12 Darwin Centenary (1909) -- 13 Chromosomes -- Part III. The Innes Years -- 14 Passages (1910-1914) -- 15 Eugenics -- 16 War (1915-1919) -- 17 My Respectful Homage (1920-1922) -- 18 Limits Undetermined (1923-1926) -- Part IV. Politics -- 19 Butler -- 20 Pilgrimages -- 21 Kammerer -- 22 Science and Chauvinism -- 23 Degrees for Women -- Part V. Eclipse -- 24 Bashing -- 25 Epilogue -- Part VI. Further Rediscovery -- 26 The Third Base -- 27 Mendel Basics -- 28 Romanes, Bateson, and Darwin's "Weak Point" -- 29 Bateson's Residue: Oligonucleotide Disharmony -- Publications of William Bateson -- References and Notes -- Acknowledgements -- Index.
Record Nr. UNINA-9910584598903321
Forsdyke Donald R.  
Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022
Materiale a stampa
Lo trovi qui: Univ. Federico II
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