Antisense RNA Design, Delivery, and Analysis / / edited by Virginia Arechavala-Gomeza, Alejandro Garanto |
Autore | Arechavala-Gomeza Virginia |
Edizione | [1st ed. 2022.] |
Pubbl/distr/stampa | New York, : Springer Nature, 2022 |
Descrizione fisica | 1 online resource (XVII, 422 p. 69 illus., 59 illus. in color.) |
Disciplina | 615.5 |
Collana | Methods in Molecular Biology |
Soggetto topico |
Therapeutics
Biomaterials Nucleic acids Nucleic Acid Àcids nuclèics RNA Teràpia genètica |
Soggetto genere / forma | Llibres electrònics |
Soggetto non controllato |
RNA therapeutics
Antisense technology Therapeutic design Oligonucleotides Model systems Oligonucleotide-mediated toxicology Open Access |
ISBN | 1-0716-2010-X |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto | Introduction and History of the Chemistry of Nucleic Acids Therapeutics -- Antisense RNA Therapeutics: A Brief Overview -- Design of Bifunctional Antisense Oligonucleotides for Exon Inclusion -- Design and Delivery of SINEUP: A New Modular Tool to Increase Protein Translation -- How to Design U1 snRNA Molecules for Splicing Rescue -- Conjugation of Nucleic Acids and Drugs to Gold Nanoparticles -- Determination of Optimum Ratio of Cationic Polymers and Small Interfering RNA with Agarose Gel Retardation Assay -- Generation of Protein-Phosphorodiamidate Morpholino Oligomer Conjugates for Efficient Cellular Delivery via Anthrax Protective Antigen -- Development and Use of Cellular Systems to Assess and Correct Splicing Defects -- Modeling Splicing Variants Amenable to Antisense Therapy by Use of CRISPR-Cas9-Based Gene Editing in HepG2 Cells -- In Vitro Models for the Evaluation of Antisense Oligonucleotides in Skin -- In Vitro Delivery of PMOs in Myoblasts by Electroporation -- Rapid Determination of MBNL1 Protein Levels by Quantitative Dot Blot for Evaluation of Antisense Oligonucleotides in Myotonic Dystrophy Myoblasts -- Evaluation of Exon Skipping and Dystrophin Restoration in In Vitro Models of Duchenne Muscular Dystrophy -- Generation of Human iPSC-Derived Myotubes to Investigate RNA-Based Therapies In Vitro -- Eye on a Dish Models to Evaluate Splicing Modulation -- Establishment of In Vitro Brain Models for AON Delivery -- Considerations for Generating Humanized Mouse Models to Test Efficacy of Antisense Oligonucleotides -- Generation of Humanized Zebrafish Models for the In Vivo Assessment of Antisense Oligonucleotide-Based Splice Modulation Therapies -- Use of Small Animal Models for Duchenne and Parameters to Assess Efficiency upon Antisense Treatment -- In Vivo Models for the Evaluation of Antisense Oligonucleotides in Skin -- Delivery of Antisense Oligonucleotides to the Mouse Retina -- Delivery of Antisense Oligonucleotides to the Mouse Brain by Intracerebroventricular Injections -- Intrathecal Delivery of Therapeutic Oligonucleotides for Potent Modulation of Gene Expression in the Central Nervous System -- Preclinical Safety Assessment of Therapeutic Oligonucleotides -- Preclinical Evaluation of the Renal Toxicity of Oligonucleotide Therapeutics in Mice -- Protocol for Isolation and Culture of Mouse Hepatocytes (HCs), Kupffer Cells (KCs), and Liver Sinusoidal Endothelial Cells (LSECs) in Analyses of Hepatic Drug Distribution -- Patent Considerations When Embarking on New Antisense Drug Programs. |
Record Nr. | UNINA-9910552988503321 |
Arechavala-Gomeza Virginia
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New York, : Springer Nature, 2022 | ||
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Lo trovi qui: Univ. Federico II | ||
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Handbook of Chemical Biology of Nucleic Acids [[electronic resource] /] / edited by Naoki Sugimoto |
Autore | Sugimoto Naoki |
Edizione | [1st ed. 2023.] |
Pubbl/distr/stampa | Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2023 |
Descrizione fisica | 1 online resource (2847 pages) |
Disciplina |
620.19
547.7 |
Soggetto topico |
Biomaterials
Nucleic acids Biochemistry Chemistry, Organic Biotechnology Nanochemistry Medicine - Research Biology - Research Nucleic Acid Chemical Biology Organic Chemistry Biomedical Research |
ISBN | 981-19-9776-4 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto | High-Pressure Single-Molecule Studies on Non-canonical Nucleic Acids and Their Interactions -- Stability Prediction of Canonical and Noncanonical Structures of Nucleic Acids -- The Effect of Pressure on the Conformational Stability of DNA -- Quadruplexes are everywhere…on the other strand too: the i-motif -- i-Motif Nucleic Acids -- NMR Study on Nucleic Acids -- Z-DNA -- Structures of DNA G-quadruplexes and Their Drug Interactions -- In cell 19F NMR for G-quadruplex -- Structures and Catalytic Activities of Complexes between Heme and DNA -- Studying nucleic acid-ligand binding by X-ray crystallography -- Predicting the 3D structure of RNA from sequence -- XNA in Synthetic Biology -- The Effects of FANA Modifications on Non-Canonical Nucleic Acid Structures -- Isomorphic Fluorescent Nucleoside Analogs -- Bridged Nucleic Acids for Therapeutic Oligonucleotides -- Mesyl Phosphoramidate Oligonucleotides: A New Promising Type of Antisense Agents -- Chemistry of Cyclic Dinucleotides and Analogs -- Labeling and Detection of Modified Nucleic Acids -- Cross linking Duplex of Nucleic Acids with Modified Oligonucleotides -- Enzymatic Synthesis of Base-Modified Nucleic Acids -- Charge Transfer in Natural and Artificial Nucleic Acids -- Nucleic Acid Aptamers: From Basic Research to Clinical Applications -- LNA in Medical Biology -- Targeting Quadruplex Nucleic Acids: The Bisquinolinium Saga -- Compound Shape and Substituent Effects in DNA Minor Groove Interactions -- Macrocyclic G-Quadruplex Ligands of Telomestatin Analogs -- Cyclic Naphthalene Diimide Derivatives as Novel DNA Ligands -- Imaging study of small molecules to G-quadruplexes in cells -- Bivalent Nucleic Acid Ligands -- DNA/Metal Cluster–Based Nano-Lantern -- Interaction of Poly (ethylene glycol)-b-poly-L-lysine Copolymers with DNA Structures: A Thermodynamic Investigation -- Chemical tools to target non-coding RNAs -- Targeting DNA junctions with small molecules for therapeutic applications in oncology -- DNA Damage and Repair in G-quadruplexes Impact Gene Expression -- DNA Structural Elements as Potential Targets for Regulation of Gene Expression -- Effects of molecular crowding on structures and functions of nucleic acids -- Structure-guided Optimization of siRNA and anti-miRNA Properties -- Tools for understanding the chemical biology of the tRNA epitranscriptome -- Sulfur and Selenium modified bacterial tRNAs -- Chemical-assisted Epigenome Sequencing -- Epitranscriptomics. Telomeres and Telomerase -- Genetic Alphabet Expansion of Nucleic Acids -- Unnatural Base Pairs to Expand the Genetic Alphabet and Code -- OGG1 at the crossroads between repair and transcriptional regulation -- Phosphorothioate Nucleic Acids - Artificial Modification Envisaged by Nature -- Aptamer Molecular Evolution for Liquid Biopsy -- Functions and Applications of Riboswitches -- PNA and Quadruplex -- Peptide Ribonucleic Acid (PRNA)-Programmed Assemblies -- Structure and Function of Aptamer and Catalytic DNA -- Origin and Evolution of RNA -- Targeting RNA with Small Molecules. |
Record Nr. | UNINA-9910736011003321 |
Sugimoto Naoki
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Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2023 | ||
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Lo trovi qui: Univ. Federico II | ||
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Low-Cost Methods for Molecular Characterization of Mutant Plants : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols / / by Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger |
Autore | Till Bradley J |
Edizione | [1st ed. 2015.] |
Pubbl/distr/stampa | Cham : , : Springer International Publishing : , : Imprint : Springer, , 2015 |
Descrizione fisica | 1 online resource (X, 35 p. 9 illus., 3 illus. in color.) |
Disciplina |
631.52
660.6 |
Soggetto topico |
Plant biotechnology
Biology—Technique Biomaterials Nucleic acids Plant Biotechnology Biological Techniques Nucleic Acid |
ISBN | 3-319-16259-4 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto | Introduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions. . |
Record Nr. | UNISA-996207426003316 |
Till Bradley J
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Cham : , : Springer International Publishing : , : Imprint : Springer, , 2015 | ||
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Lo trovi qui: Univ. di Salerno | ||
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Low-Cost Methods for Molecular Characterization of Mutant Plants : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols / / by Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger |
Autore | Till Bradley J |
Edizione | [1st ed. 2015.] |
Pubbl/distr/stampa | Cham : , : Springer International Publishing : , : Imprint : Springer, , 2015 |
Descrizione fisica | 1 online resource (X, 35 p. 9 illus., 3 illus. in color.) |
Disciplina |
631.52
660.6 |
Soggetto topico |
Plant biotechnology
Biology—Technique Biomaterials Nucleic acids Plant Biotechnology Biological Techniques Nucleic Acid |
ISBN | 3-319-16259-4 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto | Introduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions. . |
Record Nr. | UNINA-9910131313103321 |
Till Bradley J
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Cham : , : Springer International Publishing : , : Imprint : Springer, , 2015 | ||
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Lo trovi qui: Univ. Federico II | ||
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Metal-Responsive Base Pair Switching of Ligand-type Uracil Nucleobases [[electronic resource] /] / by Keita Mori |
Autore | Mori Keita |
Edizione | [1st ed. 2024.] |
Pubbl/distr/stampa | Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2024 |
Descrizione fisica | 1 online resource (137 pages) |
Disciplina |
620.19
547.7 |
Collana | Springer Theses, Recognizing Outstanding Ph.D. Research |
Soggetto topico |
Biomaterials
Nucleic acids Coordination compounds Nanotechnology Self-assembly (Chemistry) Biocompatible Materials Nucleic Acids Layer-by-Layer Nanoparticles Nucleic Acid Coordination Chemistry Self-assembly |
ISBN | 981-9994-00-4 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto | 1. General introduction -- 2. Metal-responsive DNA strand displacement reactions driven by base pair switching of 5-hydroxyuracil nucleobases -- 3. Metal-dependent base pair switching of N,N,-dicarboxymethyl-5-aminouracil nucleosides -- 4. Metal-responsive DNA tweezers driven by base pair switching of 5-hydroxyuracil nucleobases -- 5. Conclusion and perspectives. |
Record Nr. | UNINA-9910831014603321 |
Mori Keita
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Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2024 | ||
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Lo trovi qui: Univ. Federico II | ||
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Molecular Techniques for Studying Viruses [[electronic resource] ] : Practical Notes / / by Zubaida Hassan, Gulfaraz Khan |
Autore | Hassan Zubaida |
Edizione | [1st ed. 2024.] |
Pubbl/distr/stampa | Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2024 |
Descrizione fisica | 1 online resource (105 pages) |
Disciplina | 570 |
Altri autori (Persone) | KhanGulfaraz |
Soggetto topico |
Biology
Biomaterials Nucleic acids Biological assay Non-coding RNA Bioinformatics Biological Sciences Nucleic Acid Assay Systems Non-coding RNAs |
ISBN | 981-9980-97-6 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto | Chapter 1. Introduction -- Chapter 2. Isolation of Nucleic Acids -- Chapter 3. Isolation of Proteins -- Chapter 4. PCR-Based Techniques -- Chapter 5. Western Blotting -- Chapter 6. Serological Assays - Chapter 7. Immunoprecipitation -- Chapter 8. Small Interfering RNA -- Chapter 9. Histological Methods -- Chapter 10. Bioinformatics and In Silico Stimulations. Chapter 11. Summary and Conclusions. |
Record Nr. | UNINA-9910838288903321 |
Hassan Zubaida
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Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2024 | ||
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Lo trovi qui: Univ. Federico II | ||
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Nuclear, Chromosomal, and Genomic Architecture in Biology and Medicine [[electronic resource] /] / edited by Malgorzata Kloc, Jacek Z. Kubiak |
Edizione | [1st ed. 2022.] |
Pubbl/distr/stampa | Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022 |
Descrizione fisica | 1 online resource (657 pages) |
Disciplina | 571.835 |
Collana | Results and Problems in Cell Differentiation |
Soggetto topico |
Cell nuclei
Chromosomes Biology - Technique Genomics Biomaterials Nucleic acids Gene expression Nuclear Organization Genomic Analysis Nucleic Acid Gene Expression Analysis Citologia Cromosomes Genòmica Nuclis cel·lulars Diferenciació cel·lular |
Soggetto genere / forma | Llibres electrònics |
ISBN | 3-031-06573-5 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto | Part I. Genome Architecture, Evolution, and Cell Fate -- Chapter 1. Networks and Islands of Genome Nano-Architecture and their Potential Relevance for Radiation Biology (A Hypothesis and Experimental Verification Hints) -- Chapter 2. A Unified Genomic Mechanism of Cell-Fate Change -- Chapter 3. Alterations to Genome Organisation in Stem Cells, Their Differentiation and Associated Diseases -- Chapter 4. How Genomes Emerge, Function, and Evolve: Living Systems Emergence - Genotype-Phenotype-Multilism - Genome/Systems Ecology -- Chapter 5. Integrating Multimorbidity into a Whole-Body Understanding of Disease Using Spatial Genomics -- Part II. Chromosomes and Chromatin Architecture and Dynamics -- Chapter 6. Mitotic Antipairing of Homologous Chromosomes -- Chapter 7. CENP-A, a Histone H3 Variant with Key Roles in Centromere Architecture in Healthy and Diseased States -- Chapter 8. Scaling Relationship in Chromatin as a Polymer -- Chapter 9. Chromatin Dynamics during Entry to Quiescence and Compromised Functionality in Cancer Cells -- Chapter 10. Functional Aspects of Sperm Chromatin Organization -- Part III. Mechanosensitive and Epigenetic Regulators of Gene Expression and Chromatin Organization -- Chapter 11. The LINC Complex Assists the Nuclear Import of Mechanosensitive Transcriptional Regulators -- Chapter 12. Epigenetic-Mediated Regulation of Gene Expression for Biological Control and Cancer: Cell and Tissue Structure, Function, and Phenotype -- Chapter 13. Epigenetic-Mediated Regulation of Gene Expression for Biological Control and Cancer: Fidelity of Mechanisms Governing the Cell Cycle -- Chapter 14. Histone Modifications in Mouse Pronuclei and Consequences for Embryo Development -- Part IV. Nucleus, Nucleolus, and Nucleolar Organizer Architecture -- Chapter 15. Nuclear Architecture in the Nervous System -- Chapter 16. Nuclear Morphological Abnormalities in Cancer: A Search for Unifying Mechanisms -- Chapter 17. Nuclear Organization in Response to Stress: A Special Focus on Nucleoli -- Chapter 18. Simulation of Different Three-Dimensional Models of Whole Interphase Nuclei Compared to Experiment – A Consistent Scale-Bridging Simulation Framework for Genome Organization -- Chapter 19. Nucleolar Organizer Regions as Transcription-Based Scaffolds of Nucleolar Structure and Function -- Chapter 20. A Transient Mystery: Nucleolar Channel Systems -- Part V. Nuclear Actin Role in Polarization, Genome Organization, and Gene Expression -- Chapter 21. Cellular Polarity Transmission to the Nucleus -- Chapter 22. The Role of Nuclear Actin in Genome Organization and Gene Expression Regulation During Differentiation -- Chapter 23. Nuclear Actin Dynamics in Gene Expression, DNA Repair, and Cancer. |
Record Nr. | UNINA-9910629284303321 |
Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022 | ||
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Lo trovi qui: Univ. Federico II | ||
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Progress in the Chemistry of Organic Natural Products 122 [[electronic resource] ] : Botanical Dietary Supplements and Herbal Medicines / / edited by A. Douglas Kinghorn, Heinz Falk, Simon Gibbons, Yoshinori Asakawa, Ji-Kai Liu, Verena M. Dirsch |
Autore | Kinghorn A. Douglas |
Edizione | [1st ed. 2023.] |
Pubbl/distr/stampa | Cham : , : Springer Nature Switzerland : , : Imprint : Springer, , 2023 |
Descrizione fisica | 1 online resource (298 pages) |
Disciplina | 615.321 |
Altri autori (Persone) |
FalkHeinz
GibbonsSimon AsakawaYoshinori LiuJi-Kai DirschVerena M |
Collana | Progress in the Chemistry of Organic Natural Products |
Soggetto topico |
Natural products
Biomaterials Nucleic acids Chemical structure Natural Products Nucleic Acid Structure And Bonding Plant Materials |
ISBN | 3-031-26768-0 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto | Phytochemical Profiles and Biological Studies of Selected Botanical Dietary Supplements Used in the United States -- Quality Consistency of Herbal Products: Chemical Evaluation -- Deoxyribonucleic Acid Barcoding for the Identification of Botanicals -- Botanical Pharmacognosy in Quality Control: A Focus on Raw Material Assessment. |
Record Nr. | UNINA-9910734822103321 |
Kinghorn A. Douglas
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Cham : , : Springer Nature Switzerland : , : Imprint : Springer, , 2023 | ||
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Lo trovi qui: Univ. Federico II | ||
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Treasure Your Exceptions : The Science and Life of William Bateson / / by Alan G. Cock, Donald R. Forsdyke |
Autore | Forsdyke Donald R. |
Edizione | [2nd ed. 2022.] |
Pubbl/distr/stampa | Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022 |
Descrizione fisica | 1 online resource (702 pages) |
Disciplina | 575.109 |
Soggetto topico |
Genetistes
Medical genetics Medicine - History Life sciences Biology - Philosophy Evolution (Biology) Biomaterials Nucleic acids Medical Genetics History of Medicine Life Sciences Philosophy of Biology Evolutionary Biology Nucleic Acid |
Soggetto genere / forma |
Biografies
Llibres electrònics |
ISBN | 3-030-92099-2 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto | Abbreviations -- Prologue -- Part I. Genesis of a Geneticist -- 1 A Cambridge Childhood (1861-1882 -- 2 From Virginia to the Aral Sea (1883-1889) -- 3 Galton -- 4 Variation (1890-1894) -- 5 Romanes -- 6 Reorientation and Controversy (1895-1899) -- 7 What Life May Be -- Part II. Mendelism -- 8 Rediscovery (1900-1901) -- 9 Mendel’s Bulldog (1902-1906) -- 10 Bateson’s Bulldog -- 11 On Course (1907-1908) -- 12 Darwin Centenary (1909) -- 13 Chromosomes -- Part III. The Innes Years -- 14 Passages (1910-1914) -- 15 Eugenics -- 16 War (1915-1919) -- 17 My Respectful Homage (1920-1922) -- 18 Limits Undetermined (1923-1926) -- Part IV. Politics -- 19 Butler -- 20 Pilgrimages -- 21 Kammerer -- 22 Science and Chauvinism -- 23 Degrees for Women -- Part V. Eclipse -- 24 Bashing -- 25 Epilogue -- Part VI. Further Rediscovery -- 26 The Third Base -- 27 Mendel Basics -- 28 Romanes, Bateson, and Darwin's "Weak Point" -- 29 Bateson's Residue: Oligonucleotide Disharmony -- Publications of William Bateson -- References and Notes -- Acknowledgements -- Index. |
Record Nr. | UNINA-9910584598903321 |
Forsdyke Donald R.
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Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022 | ||
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Lo trovi qui: Univ. Federico II | ||
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