Antisense RNA Design, Delivery, and Analysis / / edited by Virginia Arechavala-Gomeza, Alejandro Garanto
| Antisense RNA Design, Delivery, and Analysis / / edited by Virginia Arechavala-Gomeza, Alejandro Garanto |
| Autore | Arechavala-Gomeza Virginia |
| Edizione | [1st ed. 2022.] |
| Pubbl/distr/stampa | New York, : Springer Nature, 2022 |
| Descrizione fisica | 1 online resource (XVII, 422 p. 69 illus., 59 illus. in color.) |
| Disciplina | 615.5 |
| Collana | Methods in Molecular Biology |
| Soggetto topico |
Therapeutics
Biomaterials Nucleic acids Nucleic Acid |
| ISBN |
9781071620106
107162010X |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Introduction and History of the Chemistry of Nucleic Acids Therapeutics -- Antisense RNA Therapeutics: A Brief Overview -- Design of Bifunctional Antisense Oligonucleotides for Exon Inclusion -- Design and Delivery of SINEUP: A New Modular Tool to Increase Protein Translation -- How to Design U1 snRNA Molecules for Splicing Rescue -- Conjugation of Nucleic Acids and Drugs to Gold Nanoparticles -- Determination of Optimum Ratio of Cationic Polymers and Small Interfering RNA with Agarose Gel Retardation Assay -- Generation of Protein-Phosphorodiamidate Morpholino Oligomer Conjugates for Efficient Cellular Delivery via Anthrax Protective Antigen -- Development and Use of Cellular Systems to Assess and Correct Splicing Defects -- Modeling Splicing Variants Amenable to Antisense Therapy by Use of CRISPR-Cas9-Based Gene Editing in HepG2 Cells -- In Vitro Models for the Evaluation of Antisense Oligonucleotides in Skin -- In Vitro Delivery of PMOs in Myoblasts by Electroporation -- Rapid Determination of MBNL1 Protein Levels by Quantitative Dot Blot for Evaluation of Antisense Oligonucleotides in Myotonic Dystrophy Myoblasts -- Evaluation of Exon Skipping and Dystrophin Restoration in In Vitro Models of Duchenne Muscular Dystrophy -- Generation of Human iPSC-Derived Myotubes to Investigate RNA-Based Therapies In Vitro -- Eye on a Dish Models to Evaluate Splicing Modulation -- Establishment of In Vitro Brain Models for AON Delivery -- Considerations for Generating Humanized Mouse Models to Test Efficacy of Antisense Oligonucleotides -- Generation of Humanized Zebrafish Models for the In Vivo Assessment of Antisense Oligonucleotide-Based Splice Modulation Therapies -- Use of Small Animal Models for Duchenne and Parameters to Assess Efficiency upon Antisense Treatment -- In Vivo Models for the Evaluation of Antisense Oligonucleotides in Skin -- Delivery of Antisense Oligonucleotides to the Mouse Retina -- Delivery of Antisense Oligonucleotides to the Mouse Brain by Intracerebroventricular Injections -- Intrathecal Delivery of Therapeutic Oligonucleotides for Potent Modulation of Gene Expression in the Central Nervous System -- Preclinical Safety Assessment of Therapeutic Oligonucleotides -- Preclinical Evaluation of the Renal Toxicity of Oligonucleotide Therapeutics in Mice -- Protocol for Isolation and Culture of Mouse Hepatocytes (HCs), Kupffer Cells (KCs), and Liver Sinusoidal Endothelial Cells (LSECs) in Analyses of Hepatic Drug Distribution -- Patent Considerations When Embarking on New Antisense Drug Programs. |
| Record Nr. | UNINA-9910552988503321 |
Arechavala-Gomeza Virginia
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| New York, : Springer Nature, 2022 | ||
| Lo trovi qui: Univ. Federico II | ||
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Chemical Biology of Nucleic Acids : Fundamentals and Clinical Applications / / edited by Volker A. Erdmann, Wojciech T. Markiewicz, Jan Barciszewski
| Chemical Biology of Nucleic Acids : Fundamentals and Clinical Applications / / edited by Volker A. Erdmann, Wojciech T. Markiewicz, Jan Barciszewski |
| Edizione | [1st ed. 2014.] |
| Pubbl/distr/stampa | Berlin, Heidelberg : , : Springer Berlin Heidelberg : , : Imprint : Springer, , 2014 |
| Descrizione fisica | 1 online resource (599 p.) |
| Disciplina | 572.8 |
| Collana | RNA Technologies |
| Soggetto topico |
Biomaterials
Nucleic acids Medicine - Research Biology - Research Medical genetics Nucleic Acid Biomedical Research Medical Genetics |
| ISBN | 3-642-54452-5 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | RNA as Major Components in Chemical Evolvable Systems -- How the Early Genetic Code Was Established? - Inference from the Analysis of Extant Animal Mitochondrial Decoding Systems -- Isomerization of RNA Phosphodiester Linkages -- Effects of Ionic Liquid and Liposomes on the Structure, Stability and Function of Nucleic Acids -- Oxidative Damage on RNA Nucleobases -- Use of FRET to Study Dynamics of DNA Replication -- Design, Characterization and Application of Imidazopyridopyrimidine:Naphthyridine Base-Pairing Motifs Consisting of Four Hydrogen Bonds -- Creation of Unnatural Base Pair Systems Toward New DNA/RNA Biotechnologies -- Flexible Nucleobase Analogues. Novel Tools for Exploring the Nucleic Acids -- Sequence Selective Recognition of Double-Stranded RNA -- Determining Transient Nucleic Acid Structures by NMR -- Diastereomer-Specific Repertoire of 7′R- or 7′S-Me-Carba-Locked Nucleic Acids (cLNAs) in Antisense Oligo/RNA Duplexes and Engineering of Physicochemical and Enzymological Properties -- Challenges and Opportunities for Oligonucleotide-Based Therapeutics by Antisense and RNA Interference Mechanisms -- Progress in Chemically Modified Nucleic Acid Aptamers -- Aptamers as Molecular Smugglers -- Biochemical Aspects of Subcellular RNA Transport and Localization -- Small Size, Big Impact: Bacterial Functional Nucleic Acids and Their Applications -- Towards Defined DNA and RNA Delivery Vehicles Using Nucleic Acid Nanotechnology -- Targeted Editing of Therapeutic Genes Using DNA-Based Transcriptional Activators: Scope and Challenges -- Interaction of DNA Intramolecular Structures with Their Complementary Strands: A Thermodynamic Approach for the Control of Gene Expression -- Site Directed Spin Labelling of RNA for Distance Measurements by EPR -- Chemo-Enzymatic Strategies to Modify RNA in vitro or in Living Cells -- Metal Dependence of Ligand Binding and Heavy-Atom Derivatization of Evolutionarily Distinct PreQ1 Riboswitches -- DNA G-Quadruplexes and I-Motifs in Therapeutics and Diagnostics -- Peptides Targeting G-Quadruplex Structures -- Synthesis of Site-Specifically Modified Long-Mer RNAs -- Synthesis and Exon-Skipping Activity of Chemically-Modified RNAs -- mRNA and snRNA Cap Analogs: Synthesis and Applications -- Innovative Chemistry for Synthesis of Regular RNA, 5’-Triphosphate RNA or 5’-Capped RNA. |
| Record Nr. | UNINA-9910298309903321 |
| Berlin, Heidelberg : , : Springer Berlin Heidelberg : , : Imprint : Springer, , 2014 | ||
| Lo trovi qui: Univ. Federico II | ||
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Circulating Nucleic Acids in Early Diagnosis, Prognosis and Treatment Monitoring : An Introduction / / edited by Peter B. Gahan
| Circulating Nucleic Acids in Early Diagnosis, Prognosis and Treatment Monitoring : An Introduction / / edited by Peter B. Gahan |
| Edizione | [1st ed. 2015.] |
| Pubbl/distr/stampa | Dordrecht : , : Springer Netherlands : , : Imprint : Springer, , 2015 |
| Descrizione fisica | 1 online resource (476 p.) |
| Disciplina | 612.0157 |
| Collana | Advances in Predictive, Preventive and Personalised Medicine |
| Soggetto topico |
Medicine - Research
Biology - Research Medicine, Preventive Health promotion Cancer Biomaterials Nucleic acids Pharmaceutical chemistry Biomedical Research Health Promotion and Disease Prevention Cancer Biology Nucleic Acid Medicinal Chemistry |
| ISBN | 94-017-9168-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Part I. BACKGROUND -- CHAPTER 1 Introduction - Brief History and the present and future status of CNAPS -- CHAPTER 2 The biology of CNAPS -- Part II. METHODOLOGY -- CHAPTER 3 Pre-analytical requirements for analysing nucleic acids from blood -- CHAPTER 4. Circulating DNA and miRNA isolation -- CHAPTER 5 Detection of genetic alterations by nucleic acid analysis: use of pcr and mass spectroscopy-based methods -- CHAPTER 6 Genomic approaches to the analysis of cell free nucleic acids -- Part III APPLICATIONS -- CHAPTER 7 CNAPS and General Medicine -- CHAPTER 8 Fetal CNAPS – DNA/RNA -- CHAPTER 9 Circulating nucleic acids and Diabetes mellitus -- CHAPTER 10 Extracellular nucleic acids and cancer -- CHAPTER 11 Other bodily fluids as non-invasive sources of Cell-free DNA/RNA -- CHAPTER 12 CNAPS in therapy monitoring -- Part IV OTHER ASPECTCS -- CHAPTER 13 Quality Assurance -- CHAPTER 14 Societal Aspects: Ethics. |
| Record Nr. | UNINA-9910300235703321 |
| Dordrecht : , : Springer Netherlands : , : Imprint : Springer, , 2015 | ||
| Lo trovi qui: Univ. Federico II | ||
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DNA and RNA Nanobiotechnologies in Medicine: Diagnosis and Treatment of Diseases / / edited by Volker A. Erdmann, Jan Barciszewski
| DNA and RNA Nanobiotechnologies in Medicine: Diagnosis and Treatment of Diseases / / edited by Volker A. Erdmann, Jan Barciszewski |
| Edizione | [1st ed. 2013.] |
| Pubbl/distr/stampa | Berlin, Heidelberg : , : Springer Berlin Heidelberg : , : Imprint : Springer, , 2013 |
| Descrizione fisica | 1 online resource (464 p.) |
| Disciplina |
575.2/1
612.015 |
| Altri autori (Persone) |
ErdmannV. A <1941-> (Volker A.)
BarciszewskiJan |
| Collana | RNA Technologies |
| Soggetto topico |
Medicine - Research
Biology - Research Biomaterials Nucleic acids Medical genetics Biomedical Research Nucleic Acid Medical Genetics |
| ISBN | 3-642-36853-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Introduction -- RNA Nanotechnology: methods for synthesis, conjugation, assembly and application of RNA nanoparticles -- Adsorption of double-stranded DNA to graphene oxide preventing enzymatic digestion -- Electrogenerated chemiluminescence of conjugated polymer films from patterned electrodes -- Use of RNA structure flexibility data in nanostructure modeling -- Informatic resources for identifying and annotating structural RNA motifs -- A nanostructure made of a bacterial noncoding RNA -- HAPIscreen, a method for high-throughput aptamer identification -- Characterization of photophysical and base-mimicking properties of a novel fluorescent adenine analogue in DNA -- Bifacial nucleoside as a surrogate for both T and A in duplex DNA -- Functionally important structural elements of U12 snRNA -- Self-assembling RNA nanorings based on RNAI/II inverse kissing complexes -- Automatic molecular weaving prototyped by using single-stranded DNA -- Nucleic Acid Aptamers: Clinical Applications and Promising New Horizons -- Nanotextured Substrates With Immobilized Aptamers for Cancer Cell Isolation and Cytology -- Targeting cancer with peptide aptamers -- Synthetic, biofunctional nucleic acid-based molecular devices -- Inducible site-selective bottom-up assembly of virus-derived nanotube arrays on RNA-equipped wafers -- Special delivery: targeted therapy with small RNAs, RNAi nanomedicines: challenges and opportunities within the immune system -- Pyrene-functionalized oligonucleotides and locked nucleic acids (LNAs): Tools for fundamental research, diagnostics, and nanotechnology -- Stabilizing RNA by the sonochemical formation of RNA nanospheres. Sonochemical synthesis of DNA nanospheres -- Nucleic acid based molecular devices -- Nanoparticle therapeutics: FDA approval, clinical trials, regulatory pathways, and case study -- A sol-gel-based microfluidics system enhances the efficiency of RNA aptamer selection -- Hierarchical nanotextured microelectrodes overcome the molecular transport barrier to achieve rapid, direct bacterial detection -- Recent developments in lipid-based pharmaceutical nanocarriers -- Targeted delivery of RNAi therapeutics for cancer therapy -- DNAsomes: Multifunctional DNA-based nanocarriers -- Reversible and Controllable Nanolocomotion of an RNA-Processing Machinery -- Scavenger receptors mediate cellular uptake of polyvalent oligonucleotide-functionalized gold nanoparticles -- A highly sensitive microRNA biosensor based on ruthenium oxide nanoparticle-initiated polymerization of aniline -- Preparation and high-resolution microscopy of gold cluster labeled nucleic acid conjugates and nanodevices -- Kinetically grafting G-quadruplexes onto DNA nanostructures for structure and function encoding via a DNA machine -- Synthesis of glycerol nucleic acid (GNA) phosphoramidite monomers and oligonucleotide polymers -- Cancer immunotherapy and nanomedicine -- RNA nanotechnology: inspired by DNA -- From nanotechnology to nanomedicine: applications to cancer research -- Comparative structural and functional studies of nanoparticle formulations for DNA and siRNA delivery -- Advances in novel drug delivery strategies for breast cancer therapy -- DNA origami: a history and current perspective -- Nanoparticles: a promising modality in the treatment of sarcomas -- Gold nanorod delivery of an ssRNA immune activator inhibits pandemic H1N1 influenza viral replication -- Recent developments in oligonucleotide conjugation -- In vivo delivery of small interfering RNA to tumors and their vasculature by novel dendritic nanocarriers -- DNA and carbon nanotubes as medicine -- LHRH-targeted nanoparticles for cancer therapeutics -- Nanoparticle-aptamer conjugates for cancer cell targeting and detection -- Evaluation of targets for ovarian cancer gene silencing therapy: in vitro and in vivo approaches -- Prediction and design of DNA and RNA structures -- siRNA applications in nanomedicine. |
| Record Nr. | UNINA-9910437613503321 |
| Berlin, Heidelberg : , : Springer Berlin Heidelberg : , : Imprint : Springer, , 2013 | ||
| Lo trovi qui: Univ. Federico II | ||
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Epigenetic Mechanisms of Cell Programming and Reprogramming / / edited by Gang Pei
| Epigenetic Mechanisms of Cell Programming and Reprogramming / / edited by Gang Pei |
| Edizione | [1st ed. 2023.] |
| Pubbl/distr/stampa | Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2023 |
| Descrizione fisica | 1 online resource (68 pages) |
| Disciplina | 572 |
| Altri autori (Persone) | PeiGang |
| Collana | Reports of China’s Basic Research |
| Soggetto topico |
Biochemistry
Cytology Medical genetics Biomaterials Nucleic acids Cell Biology Medical Genetics Nucleic Acid |
| ISBN |
9789811974199
9811974195 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | 1. Project Overview -- 2. Domestic and Foreign Research -- 3. Major Research Achievements -- 4.Outlook. |
| Record Nr. | UNINA-9910736984503321 |
| Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2023 | ||
| Lo trovi qui: Univ. Federico II | ||
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Functional Nucleic Acids Detection in Food Safety : Theories and Applications / / by Wentao Xu
| Functional Nucleic Acids Detection in Food Safety : Theories and Applications / / by Wentao Xu |
| Autore | Xu Wentao |
| Edizione | [1st ed. 2016.] |
| Pubbl/distr/stampa | Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2016 |
| Descrizione fisica | 1 online resource (484 p.) |
| Disciplina | 570 |
| Soggetto topico |
Biomaterials
Nucleic acids Food science Public health Nucleic Acid Food Science Public Health |
| ISBN | 981-10-1618-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | 1 Introduction: a brief guide to targets and strategies of functional nucleic acids detection in food safety -- 2 Adaptable methods to extract nucleic acid targets and evaluate quality -- 3 Basic rules in nucleic acids-mediated amplification and hybridization methods in food safety detection: a review -- 4 Reference materials: a golden criterion in nucleic acids identification -- 5 Reference gene: in-species universality versus between-species uniquity -- 6 PCR-based technologies for identifying unknown gene sequences -- 7 A developed accurate digital PCR detection technology in food safety -- 8 Development of accurate nucleic acids detection technology for target quantification -- 9 Recent progress in high-throughput detection technology for food safety -- 10 Detecting targets without thermal cycling in food: isothermal amplification and hybridization -- 11 Aptamers and their application -- 12 Lateral Flow Nucleic Acid Biosensors -- 13 Nucleic Acid Biosensors for food safety -- 14 Alternative PCR methods for detecting GM crops & food in agriculture and the food chain: a review -- 15 The identification and detection technology of research in microorganism include living or dead bacteria -- 16 Characterization of microbial diversity in food chain: a molecular review -- 17 Identification and assessment of heavy metal pollution using nucleic acid-mediated technologies -- 18 Genotoxicity detection at the molecular level in food safety assessment: conventional methods and developments -- 19 A molecular review of the detection of specific nucleic acids by amplification and hybridization Characterization of microbial diversity in the food chain: a molecular review. |
| Record Nr. | UNINA-9910253899703321 |
Xu Wentao
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| Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2016 | ||
| Lo trovi qui: Univ. Federico II | ||
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Handbook of Chemical Biology of Nucleic Acids / / edited by Naoki Sugimoto
| Handbook of Chemical Biology of Nucleic Acids / / edited by Naoki Sugimoto |
| Autore | Sugimoto Naoki |
| Edizione | [1st ed. 2023.] |
| Pubbl/distr/stampa | Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2023 |
| Descrizione fisica | 1 online resource (2847 pages) |
| Disciplina |
620.19
547.7 |
| Soggetto topico |
Biomaterials
Nucleic acids Biochemistry Chemistry, Organic Biotechnology Nanochemistry Medicine - Research Biology - Research Nucleic Acid Chemical Biology Organic Chemistry Biomedical Research |
| ISBN | 981-19-9776-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | High-Pressure Single-Molecule Studies on Non-canonical Nucleic Acids and Their Interactions -- Stability Prediction of Canonical and Noncanonical Structures of Nucleic Acids -- The Effect of Pressure on the Conformational Stability of DNA -- Quadruplexes are everywhere…on the other strand too: the i-motif -- i-Motif Nucleic Acids -- NMR Study on Nucleic Acids -- Z-DNA -- Structures of DNA G-quadruplexes and Their Drug Interactions -- In cell 19F NMR for G-quadruplex -- Structures and Catalytic Activities of Complexes between Heme and DNA -- Studying nucleic acid-ligand binding by X-ray crystallography -- Predicting the 3D structure of RNA from sequence -- XNA in Synthetic Biology -- The Effects of FANA Modifications on Non-Canonical Nucleic Acid Structures -- Isomorphic Fluorescent Nucleoside Analogs -- Bridged Nucleic Acids for Therapeutic Oligonucleotides -- Mesyl Phosphoramidate Oligonucleotides: A New Promising Type of Antisense Agents -- Chemistry of Cyclic Dinucleotides and Analogs -- Labeling and Detection of Modified Nucleic Acids -- Cross linking Duplex of Nucleic Acids with Modified Oligonucleotides -- Enzymatic Synthesis of Base-Modified Nucleic Acids -- Charge Transfer in Natural and Artificial Nucleic Acids -- Nucleic Acid Aptamers: From Basic Research to Clinical Applications -- LNA in Medical Biology -- Targeting Quadruplex Nucleic Acids: The Bisquinolinium Saga -- Compound Shape and Substituent Effects in DNA Minor Groove Interactions -- Macrocyclic G-Quadruplex Ligands of Telomestatin Analogs -- Cyclic Naphthalene Diimide Derivatives as Novel DNA Ligands -- Imaging study of small molecules to G-quadruplexes in cells -- Bivalent Nucleic Acid Ligands -- DNA/Metal Cluster–Based Nano-Lantern -- Interaction of Poly (ethylene glycol)-b-poly-L-lysine Copolymers with DNA Structures: A Thermodynamic Investigation -- Chemical tools to target non-coding RNAs -- Targeting DNA junctions with small molecules for therapeutic applications in oncology -- DNA Damage and Repair in G-quadruplexes Impact Gene Expression -- DNA Structural Elements as Potential Targets for Regulation of Gene Expression -- Effects of molecular crowding on structures and functions of nucleic acids -- Structure-guided Optimization of siRNA and anti-miRNA Properties -- Tools for understanding the chemical biology of the tRNA epitranscriptome -- Sulfur and Selenium modified bacterial tRNAs -- Chemical-assisted Epigenome Sequencing -- Epitranscriptomics. Telomeres and Telomerase -- Genetic Alphabet Expansion of Nucleic Acids -- Unnatural Base Pairs to Expand the Genetic Alphabet and Code -- OGG1 at the crossroads between repair and transcriptional regulation -- Phosphorothioate Nucleic Acids - Artificial Modification Envisaged by Nature -- Aptamer Molecular Evolution for Liquid Biopsy -- Functions and Applications of Riboswitches -- PNA and Quadruplex -- Peptide Ribonucleic Acid (PRNA)-Programmed Assemblies -- Structure and Function of Aptamer and Catalytic DNA -- Origin and Evolution of RNA -- Targeting RNA with Small Molecules. |
| Record Nr. | UNINA-9910736011003321 |
Sugimoto Naoki
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| Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2023 | ||
| Lo trovi qui: Univ. Federico II | ||
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Life by Chemical Evolution? : A Review and Evaluation of Experiments and Hypotheses / / by Hans R. Kricheldorf
| Life by Chemical Evolution? : A Review and Evaluation of Experiments and Hypotheses / / by Hans R. Kricheldorf |
| Autore | Kricheldorf Hans R |
| Edizione | [1st ed. 2025.] |
| Pubbl/distr/stampa | Berlin, Heidelberg : , : Springer Berlin Heidelberg : , : Imprint : Springer, , 2025 |
| Descrizione fisica | 1 online resource (XI, 229 p. 37 illus.) |
| Disciplina |
620.19
547.7 |
| Soggetto topico |
Biomaterials
Nucleic acids Polymers Proteins Exobiology Evolution (Biology) Nucleic Acid Protein Biochemistry Astrobiology Evolutionary Biology |
| ISBN | 3-662-70752-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Chapter 1 Introduction -- Chapter 2 Hypotheses and birthplaces of the chemical evolution -- Chapter 3 The polymerization processes of the chemical evolution -- Chapter4 Model Syntheses of Amino Acids -- Chapter 5 Model syntheses of saccharides, nucleobases, nucleosides and nucleotides -- Chapter 6 Model syntheses of oligopeptides and polypeptides -- Chapter 7 Model syntheses of oligo- and polynucleotides -- Chapter 8 Copolymerization, Self-reproduction and the genetic code -- Chapter 9 The message from space -- Chapter 10 The problem of homochirality -- Chapter 11 Summary and conclusions. |
| Record Nr. | UNINA-9911010532403321 |
Kricheldorf Hans R
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| Berlin, Heidelberg : , : Springer Berlin Heidelberg : , : Imprint : Springer, , 2025 | ||
| Lo trovi qui: Univ. Federico II | ||
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Low-Cost Methods for Molecular Characterization of Mutant Plants : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols / / by Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger
| Low-Cost Methods for Molecular Characterization of Mutant Plants : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols / / by Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger |
| Autore | Till Bradley J |
| Edizione | [1st ed. 2015.] |
| Pubbl/distr/stampa | Cham : , : Springer International Publishing : , : Imprint : Springer, , 2015 |
| Descrizione fisica | 1 online resource (X, 35 p. 9 illus., 3 illus. in color.) |
| Disciplina |
631.52
660.6 |
| Soggetto topico |
Plant biotechnology
Biology—Technique Biomaterials Nucleic acids Plant Biotechnology Biological Techniques Nucleic Acid |
| ISBN | 3-319-16259-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Introduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions. . |
| Record Nr. | UNISA-996207426003316 |
Till Bradley J
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| Cham : , : Springer International Publishing : , : Imprint : Springer, , 2015 | ||
| Lo trovi qui: Univ. di Salerno | ||
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Low-Cost Methods for Molecular Characterization of Mutant Plants : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols / / by Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger
| Low-Cost Methods for Molecular Characterization of Mutant Plants : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols / / by Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger |
| Autore | Till Bradley J |
| Edizione | [1st ed. 2015.] |
| Pubbl/distr/stampa | 2015 |
| Descrizione fisica | 1 online resource (X, 35 p. 9 illus., 3 illus. in color.) |
| Disciplina |
631.52
660.6 |
| Soggetto topico |
Plant biotechnology
Biology - Technique Biomaterials Nucleic acids Plant Biotechnology Biological Techniques Nucleic Acid |
| ISBN |
9783319162591
3319162594 |
| Classificazione | SCI007000SCI008000SCI011000 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Introduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions. . |
| Record Nr. | UNINA-9910131313103321 |
Till Bradley J
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| 2015 | ||
| Lo trovi qui: Univ. Federico II | ||
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