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Medical mycology [[electronic resource] ] : cellular and molecular techniques / / edited by Kevin Kavanagh
| Medical mycology [[electronic resource] ] : cellular and molecular techniques / / edited by Kevin Kavanagh |
| Pubbl/distr/stampa | Chichester, England ; ; Hoboken, NJ, : Wiley, c2007 |
| Descrizione fisica | 1 online resource (350 p.) |
| Disciplina | 616.96901 |
| Altri autori (Persone) | KavanaghKevin |
| Soggetto topico |
Medical mycology
Mycoses - Molecular aspects |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-282-34592-3
9786612345920 0-470-05741-6 0-470-05740-8 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Medical Mycology; Contents; Preface; List of Contributors; 1 Diagnosis of Candida Infection in Tissue by Immunohistochemistry; 1.1 Introduction; 1.2 Specificity of monoclonal antibody 3H8 for C. albicans; Protocol 1.1 Testing of specificity of monoclonal antibody 3H8; 1.3 Evaluation of monoclonal antibody 3H8 for the detection of C. albicans morphological forms; Protocol 1.2 Evaluation of monoclonal antibody 3H8 for the detection of C. albicans morphological forms; 1.4 Application of immunohistochemistry in the diagnosis of Candida periodontal disease
Protocol 1.3 Use of monoclonal antibody 3H8 in the detection of C. albicans in tissue1.5 References; 2 Transmission Electron Microscopy of Pathogenic Fungi; 2.1 Introduction; 2.2 Glutaraldehyde-potassium-permanganate or glutaraldehyde-osmiumtetroxide fixation for ultrastructural analysis; Protocol 2.1 Glutaraldehyde-osmium tetroxide (#) or glutaraldehyde-potassium permanganate (*) fixation for ultrastructural analysis; 2.3 Identification of the different compartments of the secretory pathway in yeasts Protocol 2.2 Identification of the different compartments of the secretory pathway in yeasts2.4 Cytochemical localization of acid phosphatase in yeasts; Protocol 2.3 Cytochemical localization of acid phosphatase in yeasts; 2.5 Detection of anionic sites; Protocol 2.4 Detection of anionic sites; 2.6 Detection of glycoconjugates by the periodic acid-thiocarbohydrazide-silver proteinate technique (PATAg); Protocol 2.5 Detection of glycoconjugates by the periodic acid-thiocarbohydrazide-silver proteinate technique (PATAg) 2.7 Enzyme-gold approach for the detection of polysaccharides in the cell wallProtocol 2.6 Enzyme-gold approach for the detection of polysaccharides in the cell wall; 2.8 Detection of glycoconjugates by the lectin-gold technique; Protocol 2.7 Detection of glycoconjugates by the lectin-gold technique; 2.9 Immunogold detection of antigens on ultrathin sections of acrylic resin; Protocol 2.8 Immunogold detection of antigens on ultrathin sections of acrylic resin; 2.10 Cryofixation and freeze substitution for ultrastructural analysis and immunodetection Protocol 2.9 Cryofixation and freeze substitution for ultrastructural analysis and immunodetection2.11 Overview; 2.12 References; 3 Evaluation of Molecular Responses and Antifungal Activity of Phagocytes to Opportunistic Fungi; 3.1 Introduction; 3.2 Preparation of conidia and hyphae of opportunistic fungi; Protocol 3.1 Preparation of conidia and hyphae of opportunistic fungi; Protocol 3.2 Preparation of hyphal fragments; 3.3 Isolation of human monocytes from whole blood; Protocol 3.3 Isolation of human MNCs from whole blood; 3.4 Analysis of human MNC function in response to fungal infection Protocol 3.4 XTT microassay |
| Record Nr. | UNINA-9910143737903321 |
| Chichester, England ; ; Hoboken, NJ, : Wiley, c2007 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Medical mycology [[electronic resource] ] : cellular and molecular techniques / / edited by Kevin Kavanagh
| Medical mycology [[electronic resource] ] : cellular and molecular techniques / / edited by Kevin Kavanagh |
| Pubbl/distr/stampa | Chichester, England ; ; Hoboken, NJ, : Wiley, c2007 |
| Descrizione fisica | 1 online resource (350 p.) |
| Disciplina | 616.96901 |
| Altri autori (Persone) | KavanaghKevin |
| Soggetto topico |
Medical mycology
Mycoses - Molecular aspects |
| ISBN |
1-282-34592-3
9786612345920 0-470-05741-6 0-470-05740-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Medical Mycology; Contents; Preface; List of Contributors; 1 Diagnosis of Candida Infection in Tissue by Immunohistochemistry; 1.1 Introduction; 1.2 Specificity of monoclonal antibody 3H8 for C. albicans; Protocol 1.1 Testing of specificity of monoclonal antibody 3H8; 1.3 Evaluation of monoclonal antibody 3H8 for the detection of C. albicans morphological forms; Protocol 1.2 Evaluation of monoclonal antibody 3H8 for the detection of C. albicans morphological forms; 1.4 Application of immunohistochemistry in the diagnosis of Candida periodontal disease
Protocol 1.3 Use of monoclonal antibody 3H8 in the detection of C. albicans in tissue1.5 References; 2 Transmission Electron Microscopy of Pathogenic Fungi; 2.1 Introduction; 2.2 Glutaraldehyde-potassium-permanganate or glutaraldehyde-osmiumtetroxide fixation for ultrastructural analysis; Protocol 2.1 Glutaraldehyde-osmium tetroxide (#) or glutaraldehyde-potassium permanganate (*) fixation for ultrastructural analysis; 2.3 Identification of the different compartments of the secretory pathway in yeasts Protocol 2.2 Identification of the different compartments of the secretory pathway in yeasts2.4 Cytochemical localization of acid phosphatase in yeasts; Protocol 2.3 Cytochemical localization of acid phosphatase in yeasts; 2.5 Detection of anionic sites; Protocol 2.4 Detection of anionic sites; 2.6 Detection of glycoconjugates by the periodic acid-thiocarbohydrazide-silver proteinate technique (PATAg); Protocol 2.5 Detection of glycoconjugates by the periodic acid-thiocarbohydrazide-silver proteinate technique (PATAg) 2.7 Enzyme-gold approach for the detection of polysaccharides in the cell wallProtocol 2.6 Enzyme-gold approach for the detection of polysaccharides in the cell wall; 2.8 Detection of glycoconjugates by the lectin-gold technique; Protocol 2.7 Detection of glycoconjugates by the lectin-gold technique; 2.9 Immunogold detection of antigens on ultrathin sections of acrylic resin; Protocol 2.8 Immunogold detection of antigens on ultrathin sections of acrylic resin; 2.10 Cryofixation and freeze substitution for ultrastructural analysis and immunodetection Protocol 2.9 Cryofixation and freeze substitution for ultrastructural analysis and immunodetection2.11 Overview; 2.12 References; 3 Evaluation of Molecular Responses and Antifungal Activity of Phagocytes to Opportunistic Fungi; 3.1 Introduction; 3.2 Preparation of conidia and hyphae of opportunistic fungi; Protocol 3.1 Preparation of conidia and hyphae of opportunistic fungi; Protocol 3.2 Preparation of hyphal fragments; 3.3 Isolation of human monocytes from whole blood; Protocol 3.3 Isolation of human MNCs from whole blood; 3.4 Analysis of human MNC function in response to fungal infection Protocol 3.4 XTT microassay |
| Record Nr. | UNINA-9910830383403321 |
| Chichester, England ; ; Hoboken, NJ, : Wiley, c2007 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Medical mycology [[electronic resource] ] : cellular and molecular techniques / / edited by Kevin Kavanagh
| Medical mycology [[electronic resource] ] : cellular and molecular techniques / / edited by Kevin Kavanagh |
| Pubbl/distr/stampa | Chichester, England ; ; Hoboken, NJ, : Wiley, c2007 |
| Descrizione fisica | 1 online resource (350 p.) |
| Disciplina | 616.96901 |
| Altri autori (Persone) | KavanaghKevin |
| Soggetto topico |
Medical mycology
Mycoses - Molecular aspects |
| ISBN |
1-282-34592-3
9786612345920 0-470-05741-6 0-470-05740-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Medical Mycology; Contents; Preface; List of Contributors; 1 Diagnosis of Candida Infection in Tissue by Immunohistochemistry; 1.1 Introduction; 1.2 Specificity of monoclonal antibody 3H8 for C. albicans; Protocol 1.1 Testing of specificity of monoclonal antibody 3H8; 1.3 Evaluation of monoclonal antibody 3H8 for the detection of C. albicans morphological forms; Protocol 1.2 Evaluation of monoclonal antibody 3H8 for the detection of C. albicans morphological forms; 1.4 Application of immunohistochemistry in the diagnosis of Candida periodontal disease
Protocol 1.3 Use of monoclonal antibody 3H8 in the detection of C. albicans in tissue1.5 References; 2 Transmission Electron Microscopy of Pathogenic Fungi; 2.1 Introduction; 2.2 Glutaraldehyde-potassium-permanganate or glutaraldehyde-osmiumtetroxide fixation for ultrastructural analysis; Protocol 2.1 Glutaraldehyde-osmium tetroxide (#) or glutaraldehyde-potassium permanganate (*) fixation for ultrastructural analysis; 2.3 Identification of the different compartments of the secretory pathway in yeasts Protocol 2.2 Identification of the different compartments of the secretory pathway in yeasts2.4 Cytochemical localization of acid phosphatase in yeasts; Protocol 2.3 Cytochemical localization of acid phosphatase in yeasts; 2.5 Detection of anionic sites; Protocol 2.4 Detection of anionic sites; 2.6 Detection of glycoconjugates by the periodic acid-thiocarbohydrazide-silver proteinate technique (PATAg); Protocol 2.5 Detection of glycoconjugates by the periodic acid-thiocarbohydrazide-silver proteinate technique (PATAg) 2.7 Enzyme-gold approach for the detection of polysaccharides in the cell wallProtocol 2.6 Enzyme-gold approach for the detection of polysaccharides in the cell wall; 2.8 Detection of glycoconjugates by the lectin-gold technique; Protocol 2.7 Detection of glycoconjugates by the lectin-gold technique; 2.9 Immunogold detection of antigens on ultrathin sections of acrylic resin; Protocol 2.8 Immunogold detection of antigens on ultrathin sections of acrylic resin; 2.10 Cryofixation and freeze substitution for ultrastructural analysis and immunodetection Protocol 2.9 Cryofixation and freeze substitution for ultrastructural analysis and immunodetection2.11 Overview; 2.12 References; 3 Evaluation of Molecular Responses and Antifungal Activity of Phagocytes to Opportunistic Fungi; 3.1 Introduction; 3.2 Preparation of conidia and hyphae of opportunistic fungi; Protocol 3.1 Preparation of conidia and hyphae of opportunistic fungi; Protocol 3.2 Preparation of hyphal fragments; 3.3 Isolation of human monocytes from whole blood; Protocol 3.3 Isolation of human MNCs from whole blood; 3.4 Analysis of human MNC function in response to fungal infection Protocol 3.4 XTT microassay |
| Record Nr. | UNINA-9910840502103321 |
| Chichester, England ; ; Hoboken, NJ, : Wiley, c2007 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
